In article <54420.caudle at irp.nidr.nih.gov> "caudle at irp.nidr.nih.gov" <caudle at irp.nidr.nih.gov> writes:
>On 19 Dec 1994 11:31:00 -0800,
>Fiberman <william at neuro.usc.edu> wrote:
>>>Hello Netters,
>>>>I wish to detect a receptor at the synapses of the mammalian brain. I want to
>>separate receptors at the synapse from those inside the cell (ie. those in the
>>ER, Golgi, or microsomes). If you know of a reference that talks about
>>purifying synaptic proteins, please let me know. Thanks!
>>>>-fm
>>Any general biochemistry cookbook has a recipe for isolating synaptosomes.
>However, hear is a couple of references to a reasonable procedure. Neuron
>9:929-942, 1992. PNAS 91:3954-3958, 1994.
>~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
This is the right answer to the wrong question I think - unless the intent
is to isolate presynaptic receptors. As I understand the post the author
is after postsynaptic receptors that are inserted into the surface membrane. I
do not have any answers but can we try again?
Elis Stanley
