On Thu, 22 Dec 1994 11:02:08 GMT,
elis f. stanley <elis at helix.nih.gov> wrote:
>In article <54420.caudle at irp.nidr.nih.gov> "caudle at irp.nidr.nih.gov"
<caudle at irp.nidr.nih.gov> writes:
>>On 19 Dec 1994 11:31:00 -0800,
>>Fiberman <william at neuro.usc.edu> wrote:
>>>>>Hello Netters,
>>>>>>I wish to detect a receptor at the synapses of the mammalian brain. I
want to
>>>separate receptors at the synapse from those inside the cell (ie. those
in
the
>>>ER, Golgi, or microsomes). If you know of a reference that talks about
>>>purifying synaptic proteins, please let me know. Thanks!
>>>>>>-fm
>>>>Any general biochemistry cookbook has a recipe for isolating synaptosomes.
>>However, hear is a couple of references to a reasonable procedure. Neuron
>>9:929-942, 1992. PNAS 91:3954-3958, 1994.
>>~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
~~
>>This is the right answer to the wrong question I think - unless the intent
>is to isolate presynaptic receptors. As I understand the post the author
>is after postsynaptic receptors that are inserted into the surface
membrane.
I
>do not have any answers but can we try again?
>>Elis Stanley
The Neuron paper describes isolating postsynaptic NMDA receptors from
synaptosomes.
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Robert M. Caudle "If I had my life to
NAB, NIDR, NIH live over, I'd be a
Bldg. 49, Rm 1A-11 plumber."
9000 Rockville Pike A. Einstein
Bethesda, MD 20892
Caudle at yoda.nidr.nih.gov
or
Caudle at irp.nidr.nih.gov
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
