I'm using an animal model of CNS ischemia in which the MCA is occluded and
then reperfused. What I want is a simple method to verify that he the
artery was actually occluded. I've thought about eeg, doppler, and
hydrogen clearance, but they seem quite complicated to start up. I'm
thinking about 2-DG with some sort of label, preferably non-radioactive,
that I can inject into a live animal, and locate histologically. The
problem with a simple dye is that the substance must not move around when
circulation is restored through the MCA.
Any suggestions would be appreciated.
Thanks!
Mark_Bowes at SOM-LRC.UCSD.EDU
