Dear Pam,
There has been a small amount of literature on the effects of temperature
gradients across an embryo on its development. That's a much more
complicated system, but your proposed work might provide some insights
for it, or vice versa:
Dean, J.L., M.E. Shaw & M.A. Tazelaar (1928). The effect of a temperature
gradient on the early development of the frog. Br. J. Exp. Biol. 5,
309-336.
Gilchrist, F.G. (1928). The effect of a horizontal temperature gradient
on the development of the egg of the urodele, Triturus torosus. Physiol.
Zool. 1(2), 231-268.
Glade, R.W., E.M. Burrill & R.J. Falk (1967). The influence of a
temperature gradient on bilateral symmetry in Rana pipiens. Growth 31,
231-249.
Huxley, J.S. (1927). The modification of development by means of
temperature gradients. Wilhelm Roux' Arch. Entwicklungsmechanik 112,
480-516.
I've collected these old references, but haven't obtained the papers. In
exchange for the information, if you dig them out, how about sending me a
copy?
Best regards,
-Dick Gordon[Nov24,94]
Dr. Richard Gordon
Professor, Department Of Radiology, University of Manitoba
Room ON104, Health Sciences Centre, 820 Sherbrook Street
Winnipeg, MB R3A 1R9 Canada
Phone: (204) 787-1076, Fax: (204) 783-8565, E-mail: GordonR at cc.UManitoba.ca
On 23 Nov 1994 davidson at umbc.edu wrote:
> Hello, I am a graduate student at the University of Maryland. Currently,
> I am trying to develop a method to separate axonally transported vesicles
> based on transit rates. I would like to separate fast axonal transport
> vesicles that translocate in the lower range (ie ~200 mm/day) from those
> that translocate in the upper range (ie 400mm/day). I have been thinking
> about using a temperature step gradient. My initial thinking was that
> maybe by cooling the axons in a gradient fashion I could stop the slower
> moving vesicles at a higher temp than the faster moveing ones. However I
> have not found any literature examining the effect of temperature (ie
> cold block) on differentially slowing of translocating vesicles. So I am
> back to square one. Does anyone have any suggestions as to which
> direction I should go in order to accomplish this? Any help would be
> greatly appreciated. My e-mail address is davidson at umbc.edu.> Thank you
> Pam Davidson
>>
