See paper by Stoppini ?. He used interface culture using Costar membrane
inserts to keep slices alive days to weeks. Paper about 1991.
Marc C. Brande, M.S. SD3D Email List:3D Imaging
San Diego 3D Imaging Group To subscribe/unsubscribe:listserv at bobcat.etsu.edu
3840 Camino Lindo To post a message:sd3d at bobcat.etsu.edu
San Diego, CA 92122 Email:BRANDE at SDSC.EDU Voice:619-587-4830
On 29 Nov 1994, Heinz Beck wrote:
>> I once heard from a friend, who had heard from a friend etc. that there is
> a method for keeping brain slices of hippocampus or cortex alive for a long
> time i.e. about 24 hours. My understanding was this was achieved by substi-
> tuting sachharose for sodium in the extracellular solution during the pre-
> paration but I could not locate an exact source of information or a protocol
> up to now. Anyone have information or protocols about long-term maintenance
> of brain tissue slices in vitro?
>> We have been doing our preparations with a vibratome and cold high Magnesium -
> Low Calcium ringer. Storage was submerged in standard ACSF at room temparature.
> Measurements were done using a Haas-type interface chamber. This has kept our
> slices viable for about 12 hours.
>> As we are beginning to work on human tissue now, I have been thinking about
> adding some sort of free radical scavenger enzyme to the preparation ringer
> to possibly lighten the effects of ischemia during explantation of the tissue.
> Has anyone tried doing this in animal preparations?
>> Thanks
> Heinz
>
