In article <pataky-2911941510090001 at steevlab.generes.ca>,
pataky at bcu.ubc.ca (Dave Pataky) wrote:
> Hey neuron culturists,
>> I have started to culture primary neurons from embryonic chick brain, and
> would like to coat the wells with polylysine, then laminin. I'm looking
> for a recipe for borate buffer for making up the polylysine, as i've heard
> this is the buffer of choice. Anyone have such a thing, maybe even know
> why use borate buffer? Any tips or alternatives would be greatly
> appreciated. Thanks in advance,
>
We grow sympathetic and hippocampal neurons on polylysine and laminin, and
we just dissolve the polylysine in phosphate-buffered saline (e.g.
Dulbecco's PBS) at 1 mg/mL.
> --
> Dave Pataky
> Dept of Zoology, UBC
>pataky at bcu.ubc.ca
--
Hannah Dvorak |
DvorakH at starbase1.caltech.edu |
Division of Biology 156-29 | Ceci n'est pas un .sig.
California Institute of Technology |
Pasadena, CA 91125 |
