I am starting some experiments with cultured neurons (from embryonic chick
brain) and thought it would be neat to test the effects of a brain extract
prepared from embryonic chick. I tried preparing some with a simple
protocol i got out of a published paper but it seemed to have a
detrimental effect on my cultures. I was hoping for some sort of trophic
effect. Any suggestions or tips or detailed protocols on preparing such a
trophic cocktail from embryonic brain would be greatly appreciated, via
the newsgroup or e-mail. i will summarize the replies here if there are
enough of them. Thanks in advance,
--
Dave Pataky
Dept of Zoology, UBC
pataky at bcu.ubc.ca
