In article <3i9s7o$dtl at dux.dundee.ac.uk>, (k.c.breen at dundee.ac.uk) wrote:
> One method to differentiate neuroblastoma cells is to treat the cells with
> retinoic acid (RA). I have seen two protocols; one uses RA in ethanol, the
> other RA in DMSO. Both are made up at a stock of 10-2M and then diluted 1
> in 1000 when treating the cells. Which method is preferable, or is there
> any particular difference? Furthermore, I believe that RA is
> light-sensitive and the solution is unstable. Would anybody have
> suggestions for storage conditions, and is is sensitive to repeated
> freeze-thaw cycles?
Try sending e-mail to my father at CHLA. About 20 years ago, he did his
dissertation on the use of RA to alter the pace of the cell cycle; this has
started some ferocious arguments between us, notably when I was on a round
of Accutane during my senior year of high school. Anyhow, if you e-mail me
at tjt3 at cornell.edu, I will send you his e-mail address. I'm not going to
post it where Molecule One or any of the other clowns that sometimes pop up
on this newsgroup can see it, but if any other researchers need info on RA
vehicles/storage and related topics, I will gladly give the address to them
as well. I just can't go posting his address (electronic that is) on the
Internet; he'd probably tear me a new...well, you know.
I can tell you myself that solutions of RA are definitely unstable, and I'm
not sure if the DMSO protocol sounds appropriate. But my father will know
better. Oh, and by the way, he is still very active in oncogene/cancer
research. It's not like he suddenly gave up after doing his thesis! So he
should be caught up on whatever the accepted methods, drawbacks, etc. are
for either protocol.
I tried to e-mail this to you, but my FTP server did not recognize your
address. Sorry!
X+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+oX
Xo Timothy J. Triche, Jr. <tjt3 at cornell.edu> aka Jabbo the Blind Pimp +X
X+ Rumors are Treason and make The Computer unHappy...have a Nice Day oX
Xo+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+o+X
