IUBio

Seasonal Xenopus oocyte expression?

GS Hamilton hamilton at umbc.edu
Tue Feb 28 21:30:20 EST 1995


In article <3ivkks$csk at news.service.uci.edu>,
 <qnguyen at darwin.bio.uci.edu> wrote:
>	Dear Netters,
>
>It seems that there is some kind of seasonal variation in the expression potency of Xenopus oocytes
>expressed with brain mRNA. Since my oocytes haven't been expressing well over the past several
>weeks, I wonder if I can blame the cells (instead of me)... The expression of GABAA receptors is
>particularly poor.
>
>I'd like to know if there are people currently facing the same problem. If not, could you mention the
>name of your Xenopus supplier?

     It's not just you. Seasonal variation in growth/expression potential 
of Xenopus oocytes is well-known to regular users.
gs Hamilton





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