I am looking to correspond with any- and everyone who uses Lucifer Yellow to
fill neurons (and perhaps other cells).
In my lab I am using LY to identify and inactivate cells in the buccal
ganglion of the sea mollusc _Aplysia californica_, and have been having
some... inconstancy... in the results.
No tidbit is too trivial. Any of _your_ rules of thumb will certainly be
appreciated. For the record, we use 1.0mm thin-wall capillary tubing from AM
Systems, pulled on a Flaming-Brown pipette puller manufactured by Sutter
Instruments. Our LY is from Molecular Probes and the intracelular amp is also
from AM Systems. We inactivate the dye (and the cell) by exposure to 500nm
blue light from a Zeiss fluorescent scope.
If there is any interest, I will post a summary of responses.
jon
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Jonathan Jacobs jxj24 at po.cwru.edu
<--
jacobs at cwbiol2.biol.cwru.edu
Department of Biomedical Engineering
Case Western Reserve University Rm. 300, Biology Building
Cleveland, OH 44106-7080 216.368.3574 (lab)
