Patrick Martin (patrick at anatomy.ucl.ac.uk) wrote:
: Does anyone have a nice recipe for a solution that could be used to stick brain
: slices to slides? I am currently using gelatin coated slides but when I dehydrate
: the sliced brains prior to staining they float off the slides with disconcerting
: regularity.
: The brains are from perfused rats, soaked in 30 % sucrose formalin to force out
: water, blocked, bonded to a post with tissue tek, and cut coronally at -10 degrees
: on a cryostat.
: Thanks in advance.
: Patrick
I've found that cryostat -generated slides stick nicely to Superfrost
slides (from Fisher), untraeted (use straight out of the box).
Jason Dugas
