Jonathan Jacobs (jxj24 at po.cwru.edu) wrote:
: I am looking to correspond with any- and everyone who uses
: Lucifer Yellow to fill neurons (and perhaps other cells).
: In my lab I am using LY to identify and inactivate cells in the buccal
: ganglion of the sea mollusc _Aplysia californica_, and have been having
: some... inconstancy... in the results.
: No tidbit is too trivial.
Are you aware that some LY forms precipitate with K+ ions?
Have you bought the type that doesn't? We had a difficult
time filling cells until we saw the orange LY precipitate
at the tip of the electrode. Reversing the current (to pos)
iontophoresed the K out of the electrode, but of course this
pulled LY back in.
Rob Smith
