Chou Hung <cph at cco.caltech.edu> writes:
Hello everyone,
I've written a LabVIEW program for single- and two-electrode
extracellular, intracellular, and whole-cell recording. The program is
currently set up for episodic recording from CA1 hippocampal neurons, but it
should work for most neurons, especially slice preparations. If you're
interested, let me know. Cheers.
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Temporary name: Cornu Ammonis (this is too specific, and I welcome any
suggestions)
Function: Record and analyze two-electrode episodic extracellular,
intracellular, and whole-cell recordings
Suggested hardware & software: Pentium with 16 Mb RAM, math coprocessor (req),
LabVIEW 3.1 for Windows (National Insturments),
National Instruments' A/D board AT-MIO-16DH,
National Instruments' BNC board BNC-2080,
Axon Instruments' Axoclamp 2B
Axon Instruments' Isolator 11
Microcal's Origin (for nicer graphs)
CA can be easily reconfigured for Macs & Sparcs
Features: record from up to two recording electrodes
extracellular, intracellular, and whole-cell recording combinations
software-controlled stimulus output (2 stimulating electrodes)
tetanus trains, Stoppini tetanus, 1-Hz stimulation
test for independent pathways, paired-pulse facilitation (PPF)
analyze initial slope, amplitude, baseline shifts, mEPSC freq &
amplitudes, series & membrane resistance,
average over experiments
average and/or display multiple sweeps
adjust for baseline, discard values over a range, change timebase
find average, SD, and Standard Error of Measurement (SEM) over range
create histogram, plot cumulative probability, apply K-S test
export data to Origin or Excel
pop-up graph for recording where stim & recording electrodes are
placed
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Chou Hung cph at cco.caltech.edu
Schuman Lab 818-395-1426
California Instute of Technology
