In article <D2MBy3.M2q at freenet.carleton.ca>, bm189 at FreeNet.Carleton.CA (Dan Small) says:
>>I am trying to decide on a glucose concentration during an in vitro
>ischemic insult to rat brain hippocampal slices. Should I go from
>10 mM to 2 mM, 10mM to 0 mM or 5 mM to 2 mM or 5 mM to 0 mM or
>or just keep 4 mM right through and not drop the glucose at all?
>Does anyone know the concentration of glucose in the brain in vivo
>during a global ischemic insult? Any help would be appreciated. Thanks.
>Cheers,
>Dan Small
>Small at biologym54.lan.nrc.ca
The brain is very rapidly depleted of its stores of glucose and glycogen
during ischemia. In our own experiments with brain slices from human brains
(obtained at neurosurgery for tumors) we have therefore chosen to go from
10 mM to 0 mM in one step. Pure anoxia will probably induce the same damage
as combined anoxia an glucose deprivation though, and for the same reason:
Glycolysis alone rapidly fails to support energy demands. Of course there
may be fine differences between anoxia and ischemia that are not covered
here. Especially when working with cultured cells one should be aware that
these may depend more on glycolysis relative to oxidative phosphorylation
than brain slices or intact brain.
Jon Henrik Laake, MD
---------------------------------------------------------------
Anatomical Institute, University of Oslo,
POBox 1105 Blindern,
0317 OSLO, NORWAY
Tel +47 22851176/51150, FAX +47 22851278,
EMail: jon.laake at basalmed.uio.no
