In article <Pine.3.05.1.9501271652.A17328-a100000 at pauline.sdsc.edu>,
brande at SDSC.EDU (Marc Brande) wrote:
> Type 1 astros are GFAP+/A2B5-.GFAP=glial acidic fibrillary protein.Both
> Abs are available try Sigma, or Boeringer Mannheim
>
Not necessarily. A very small proportion of type-1 astrocytes isolated
from rat optic nerve express A2B5 in culture, and many type-1's can
up-regulate this marker with extended culture. A2B5 doesn't work properly
on sections (unless you inject A2B5 hybridomas into the animal before
sacrifice), and GFAP can be expressed at very low levels in vivo.
Andy
--
Andy Groves
Division of Biology, 216-76
California Institute of Technology
