>It seems that there is some kind of seasonal variation in the expression potency of Xenopus oocytes
>expressed with brain mRNA. Since my oocytes haven't been expressing well over the past several
>weeks, I wonder if I can blame the cells (instead of me)... The expression of GABAA receptors is
>particularly poor.
>>I'd like to know if there are people currently facing the same problem. If not, could you mention the
>name of your Xenopus supplier?
>
Although seasonal variation is to be expected, you also rule out
other factors such as environment and disease.
Our lab had expression problems for many weeks last year. Turned out
to be the light/dark cycle. The light timer in the frog room broke
and nobody noticed 'cause nobody ever went up there at night.
Things came back to normal quickly once the timer was fixed.
Hope you get it resolved,
Dan.
