Does anyone have experience with transient transfection of neurons
in primary culture? I am interested in introducing a series of
b-gal reporter constructs into cortical neurons in established cultures
(i.e., from day E15-17 gestation rats, maintained for 3-5 weeks).
Specific questions:
What methods seem to work?
What is a good normalization control?
What control promoters work well?
Any advice will be appreciated.
Thanks,
Matthew Frosch
frosch at dsg.harvard.edu
