I hope to do a bio. project on how NCAMs act as guidance
cues for growth cones. Does anyone have ideas on what
methods I could use to determine the NCAM/growth cone
connection? So far I've thought of immunofluorescence
imaging, to show that NCAMs are there; Maybe clipping off
the NCAMs to see what effect it has on growth cone guidance
(what enzyme would do this?); Adjusting the sialic acid
content on the NCAM and see what this would effect (again,
how could I get rid of the sialic acids on the NCAM?).
Any ideas/suggestions/comments would be greatly appreciated.
Thanks
Dave
