I'm looking to measure concentrations of glucose in the
0.1 - 3 mM range, with a sample size (before any dilution) of
~10ul. Other than setting up a ferrocene electrochemical
detection assay (Fillenz, Boutelle et al. type), do folks have
recommendations for quantification methods to look at? I
guess that it will have to be enzymatic to ensure the
specificity given that the samples will be taken in vivo.
[One recommendation I have received is to use an NADPH
fluorometric method - if you have a reference/details on this,
they would also be useful. The original assay I tried, based
on the Sigma oxidase/peroxidase kit, won't go below 3mM.]
Thanks all.
--
Ewan McNay - ewan at virginia.edu / (804) 296-2089 or 982-4753 (lab)
Gamer/socialist/neuroscientist/cook (not necessarily in that order)
"For the triumph of [the right], it is necessary only that
good men do nothing"
