XZHENG at GAMMA.IS.TCU.EDU wrote:
>> I am a graduate student working on 3H-NA uptake by synaptosomes. I was told
> that 3H on the phenyl ring of NA can be converted into 3H-water so the
> counting may not reflect the total NA taken up by synaptosomes. Any reference
> on this issue? Or should I use [14C]-NA instead?
> Alan Zheng
While it is true that some exchange may take place between the tritium
on the ring and the aqueous environment, this should not cause a significant
problem in your uptake experiments if you employ the appropriate blanks.
If the exchange has taken place prior to adding it to your samples, you
can evaporate a sample of the tritiated NA before using it. You could, infact,
use 14C-NA as well, but depending on specific radioactivities and the
concentration that you desire, you may be limited to tritium.
Good luck.
J.R. Simon
