IUBio

testosterone

Kent Nastiuk, Ph.D. nastiuk at rockvax.rockefeller.edu
Wed Oct 4 16:51:53 EST 1995


mantei at neuro.biol.ethz.ch (Ned Mantei) wrote:
>I want to measure testosterone and corticosterone levels in small amounts
>(20-30 ul) of plasma taken from mice tails. Does anyone have experience
>with such measurements in mice and can recommend a RIA kit or other ?
>
>Oliver Stork
>Dep. of Biology
>ETH Zürich, Switzerland
>
>-- 
>Ned Mantei
>Neurobiology, Swiss Federal Institute of Technology
>CH-8093 Zurich, Switzerland
>mantei at neuro.biol.ethz.ch; fax: +41-1-633-1046

i used the method from the enclosed reference to measure steroid levels 
from small volumes of plasma.  i modified the method for single steroid 
determinations by eliminating the column chromatography, and the results 
were fine.


TY  - JOUR
ID  - 000831
AU  - Wingfield,J.C.
AU  - Farner,D.S.
PY  - 
1975
SP  - 311
EP  - 327
KW  - ria
KW  - steroid
KW  - androgen
KW  - 
estrogen
KW  - progesterone
KW  - methods
KW  - bird
RP  - In File
TI  - 
The determination of five steroids in avian plasma by radioimmunoassay 
and competitive protein-binding
JO  - Steroids
VL  - 26
ER  - 




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