mantei at neuro.biol.ethz.ch (Ned Mantei) wrote:
>I want to measure testosterone and corticosterone levels in small amounts
>(20-30 ul) of plasma taken from mice tails. Does anyone have experience
>with such measurements in mice and can recommend a RIA kit or other ?
>>Oliver Stork
>Dep. of Biology
>ETH Zürich, Switzerland
>>--
>Ned Mantei
>Neurobiology, Swiss Federal Institute of Technology
>CH-8093 Zurich, Switzerland
>mantei at neuro.biol.ethz.ch; fax: +41-1-633-1046
i used the method from the enclosed reference to measure steroid levels
from small volumes of plasma. i modified the method for single steroid
determinations by eliminating the column chromatography, and the results
were fine.
TY - JOUR
ID - 000831
AU - Wingfield,J.C.
AU - Farner,D.S.
PY -
1975
SP - 311
EP - 327
KW - ria
KW - steroid
KW - androgen
KW -
estrogen
KW - progesterone
KW - methods
KW - bird
RP - In File
TI -
The determination of five steroids in avian plasma by radioimmunoassay
and competitive protein-binding
JO - Steroids
VL - 26
ER -