NB: use email address in .sig ONLY
G'Day Folks
XZHENG at GAMMA.IS.TCU.EDU writes:
>I am a graduate student working on 3H-NA uptake by synaptosomes. I was told
>that 3H on the phenyl ring of NA can be converted into 3H-water so the
>counting may not reflect the total NA taken up by synaptosomes. Any reference
>on this issue? Or should I use [14C]-NA instead?
>Alan Zheng
No, this is not correct. Ring 2,5,6 [3H]-NA is probably the best labled
compound for uptake and release studies, and our group use it whenever cost
allows. Ring H has very low exchange rates with H in the medium, and loss of
radioactivity by that route is low. Many groups, including our own, have
repeatedly show that disposition and release of radiolabeled NA parallels
that of endogenous NA. There are many references, but most are in the late
70's to mid 80's, easy to miss on a medline search. You may want to look up
Trendelenberg, U for lots of work on NA uptake in various tissues, and good
pointers to other work. Early work by Starke, Langer and Majewski will also
be handy (I can't give more precise references as I don't have my reference
list handy, I'm currently in a different lab).)
Cheers! Ian
