IAN-MU at med.monash.edu.au (Ian Musgrave) wrote:
> I have been trying to dissociate hippocampal
>neurons for FURA2 intracellular calcium studies, and I can obtain NO viable
>cells at all. Similar methods used on pituitaries results in about 90% viable
>cells. I am at my wits end, any suggestions?
>>Cheers! Ian
>>-------------------------------------------------------------
>Ian Musgrave PhD. Prince Henry's Institute of Medical Research
>Mail: PO Box 5152 Clayton, Vic 3168, Australia. FAX: +61 3 550 6125
>E-mail: Ian.Musgrave at med.monash.edu.au Phone: +61 3 550 4286
You might get some ideas from this paper:
Mechanical and enzymatic isolation of mammalian CNS neurons.
Kaneda-M; Nakamura-H; Akaike-N
Neurosci-Res. 1988 Apr; 5(4): 299-315
---------------------------------
Mark Farrant
Department of Pharmacology
University College London
---------------------------------
