G'Day Folks
(Ren-Shiang Chen) writes:
>From: crs at ntumc1.mc.ntu.edu.tw (Ren-Shiang Chen)
>Subject: Fresh dissociated neurons --problems!
>Date: 3 Oct 1995 14:17:12 GMT
>First time post here, any inappropriateness please forgive me.
>I wonder if anyone can give me some suggestion about preparing fresh
>dissociated rat hippocampal neurons. My lab is proforming whole-cell
>recording on them for study drug kinetics.
[questions snipped]
I too would like some pointers, I have been trying to dissociate hippocampal
neurons for FURA2 intracellular calcium studies, and I can obtain NO viable
cells at all. Similar methods used on pituitaries results in about 90% viable
cells. I am at my wits end, any suggestions?
Cheers! Ian
-------------------------------------------------------------
Ian Musgrave PhD. Prince Henry's Institute of Medical Research
Mail: PO Box 5152 Clayton, Vic 3168, Australia. FAX: +61 3 550 6125
E-mail: Ian.Musgrave at med.monash.edu.au Phone: +61 3 550 4286
