In article <DGJtJu.HJs at psych.toronto.edu> scott at psych.toronto.edu (Brian Scott) writes:
>From: scott at psych.toronto.edu (Brian Scott)
>Subject: Ethanol fixation in BrdU immunohistochemistry?
>Date: Mon, 16 Oct 1995 15:51:09 GMT
>Hello all,
>I've been looking into bromodeoxyuridine immunohistochemistry for labeling
>neuronal precursors and I've noticed that many of the papers mention using
>ethanol to fix the tissue and not something like paraformaldehyde. I've been
>given a method to follow which uses Lana's fixative which includes
>paraformaldehyde and picric acid (yikes!) and I've been wondering if ethanol
>would do just as well. I've never heard of just using ethanol before. Does
>anyone know if it's ok? Thanks.
I don't know about straight ethanol, but we've used 1 part glacial acetic acid
to 3 parts absolute ethanol, which works fine.
