A year or so ago I evaluated a couple two-electrode oocyte clamps for our lab,
and gained some interesting insights into the performance of TEV clamps on
Xenopus oocytes. I created a couple documents for our lab's use.
Looking at the zillions of papers and abstracts using the technique, I thought
my observations might be useful to others, hoping to get the best data they
can. The documents (which I call 'The TEV Report' and 'The TEV Report: part
II') describe some serious limitations of TEV clamping on oocytes, and how to
get around some of them.
I have installed the information on the web:
http://cc.usu.edu/~davef/tev/tev.html
If you do two-electrode voltage clamping on Xenopus oocytes (or are just
interested in the technique), you might want to take a look.
I hope the information is useful.
I welcome suggestions/comments.
-Dave Featherstone
davef at cc.usu.edu
