I am currently involved in a study as an undergraduate concerning the
immunomodulating effects of pleasure center stimulation. I am familiar
with studies showing increased antibody response to antigen when rats
were allowed to electrically self stimulate or were
electrically stimulated in various pleasure centers. I am not;however,
familar with studies showing increased antibody response when pleasure
centers were chemically stimulated. We have set up a procedure which
would use miniosmotic pumps to continually pump dopamine into the brain
of rats. I have run across a few questions which I hope can be
answered on this post.
1. Is anyone familiar with similar research?
2. We intend to insert the cannula into the nucleus accumbens- are there
any preferred stereotaxic coordinates?
3. We intend to run the pumps for 3 weeks at a rate of .25 micro L /hr.-
are there any suggested dopamine concentrations- my only references on
this are Costall et al. in which locomotor response was studied.
4. How likely is dopamine to cross the blood/brain barrier?... and effect
peripheral immune responses?
5. Would dopamine/CSF- 0.1% sodium metabisulphite aliquots be stable
frozen over a long period?
Thanks in advance,
Todd
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- Todd D. Gould e-mail: tgould at falcon.lhup.edu -
- student, Lock Haven University tel : (717) 748-0933 -
- Lock Haven, PA 17745 -
- correspondence: 500 West Church street -
- Apartment 125 -
- Lock Haven, PA 17745 -
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"What is mind?"
"No matter."
"What is matter?"
"Never mind."
C.S. Sherrington
