In article <DFH2r4.8p8 at psych.toronto.edu>, scott at psych.toronto.edu
says...
>>Hello,
>>can anyone suggest an agent which can selectively halt mitosis in neural
>precursor (stem cells) in vivo, leaving differentiated neurons ok?
>Temporarily or permanently; both are fine. I hope there exists such a
>beast, which will have few side effects. Thanks.
>>Brian
When culturing neurons, it is common to use anti-mitotic agents to remove
the glia and fibroblasts. I would guess this would work in your situation as
well.
Typically, ARA-C (ctyosine arabinoside) and 5-FU (5-fluorouracil) are used.
See if you can find the text on culturing neurons edited by Banker.
Jody K. Hirsh
Northwestern University, Chicago, IL. USA
jkh141 at nwu.edu
