IUBio

Tissue embedding/sectioning

Kalman Rubinson rubinsnk at is2.nyu.edu
Sat Jun 22 09:57:49 EST 1996


meeks joshua james (j-meeks at students.uiuc.edu) wrote:
: Is it possible to parrifin embed tissue and still do frozen sections? 
: What kind of fix would you suggest?

1. If you freeze paraffin, it gets very brittle.
2. Why would you want to?  Paraffin embedding followed by frozen
sectioning?  One could do frozen sections first,
for histochemistry that would not work after dehydration and paraffin
embedding, followed by re-embedding in paraffin for re-sectioning.  I
still don't see why.

Kal




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