Stephen Black (sblack at HERA.UBISHOPS.CA) wrote:
: As a graduate student many years ago in a former life, I had occasion to
: to slice rabbit brains, and I found their large size difficult to handle
: for transfer to slides. I solved the problem by first embedding the brains
: in gelatin and then doing frozen sectioning. There's probably a recipe
: somewhere for doing this, but it's not difficult to make one up on your
: own.
Sorry but that's irrelevant. So-called gelatin-embedding for frozen
sections is really gelatin-encasing which helps support the tissue during
cutting and handling. Egg-yolk works even better. Did both of these for
years.
OTOH, paraffin embedding is something else as paraffing becomes quite
difficult to cut when frozen: It shatters. Some have added rubber cement
to the paraffin but that only helps a bit.
So, the question remains: why would one want to paraffin embed for frozen
sections?
Kal
