Dear sirs,
I am a Biologist who will start a PhD in Molecular Neuropharmacology
this fall. I am very interested in the use of transgenic mice (specially the
use of embryonic stem cells as a vehicle to mutate specific genes). I do
have some protocols but I have one question that seems to remain
unanswered. After pro nuclear microinjection how does the DNA template
integrate the host genome. Is it by homologous recombination? I would
like to know what the exact mechanism is. Could please shed some light in
this subject for me?
Thank you in advance for your replies
