I am trying to amplify the 5HT-2A mRNA from total rat platelet RNA with the
RT-PCR method. I have just once obtained a faint band on EtBr agarose gel.
Even though I used the same protocol for other samples, my labor has been
in vain.
Can anyone who succeeded more than once in a similar experiment contact me
and give me a few tips. I'd be very grateful
