Hi everyone!
I am a portuguese Ph.D student and I'm trying
to stain big and medium-sized DRG neurons with
NF200 (N52) antibody using the ABC technique.
The problem is that the antibody is made in
mouse and I'm using mouse tissue. I have already
bought the Mouse on Mouse Kit from Vector but
I am still having problems. I first tryed diluting
the antibody 1/500 and 1/1000. Since I got
a lot of background, I tryed diluting it in
PBST and incubating at 4ºC. Still bad. I've
tryed blocking with glycine and normal goat
serum. Worse!
I'm really running out of ideas. Can anyone
give me a hint (as soon as possible, please)on
how to proceed?
Many thanks to all,
Célia
