Dilute even more? use eg. 1/3000 & 1/10,000?
This does seems to be the toughest step in geting these kits running.
Goood luck
Justus
On 3 Sep 2001 19:51:45 GMT, <celiacruz at netc.pt> wrote:
>>Hi everyone!
>>I am a portuguese Ph.D student and I'm trying
>to stain big and medium-sized DRG neurons with
>NF200 (N52) antibody using the ABC technique.
>The problem is that the antibody is made in
>mouse and I'm using mouse tissue. I have already
>bought the Mouse on Mouse Kit from Vector but
>I am still having problems. I first tryed diluting
>the antibody 1/500 and 1/1000. Since I got
>a lot of background, I tryed diluting it in
>PBST and incubating at 4ºC. Still bad. I've
>tryed blocking with glycine and normal goat
>serum. Worse!
>>I'm really running out of ideas. Can anyone
>give me a hint (as soon as possible, please)on
>how to proceed?
>>Many thanks to all,
>Célia
