Bill wrote:
> Hi Guys,
>> I thought I would post the result of a little experiment I tried
> today. After reading:
>http://www.precisionary.com/products_faq.html#q9> I tried making some cortical slices (250um thick) in a cutting chamber
> heated with a 35 degree water bath (in sucrose aCSF) from 20 day old
> tissue. While the brain was so much more fluid than at 4 degrees, but
> the cells were wonderfully visible and healthy looking.
> Just a suggestion that I thought someone might be interested in.
Hi Bill,
I though already about cutting with a warm bath... but I did not want to
risk... on the other hand P20 are quite easy to get, what about older
animals? (ie. 1/2 months?)
