[Neuroscience] Problems with transfection: eGFP - mCherry interactions?

[Tom]

Hi!

Did anybody ever tried co-expression of eGFP and mCherry in cultured
hippocampal neurons?

I tried this now repeatedly using CaPhos- and lipofectamine protocols
but i never found a nice red fluroescence.
No problems occured when i co-transfected eGFP and mRFP or with singel
transfection of just mCherry.

Now why won't the eGFP/mCherry co-transfection work?

Thanks for comments!

Regards,
Thomas