The ITS2 sequence of Nematodes has been used to show the
relatedness among a few nematode species in the past. At present, I do
not have access to those articles and was wondering if someone out there
could tell me what primers were used. Were the Vrain's primers
successful? At present, I am trying to use the Vrain's primers to amplify
the ITS2 sequence in Camallanus and Spirocamallanus (Nematoda: Spirurida:
Camallanidae) that I collected from French Polynesia.
Please send replies to m.rigby at ualberta.ca or the address on this
post (mrigby at gpu.srv.ualberta.ca).
Thanks in advance.
Department of Biological Sciences
University of Alberta
Canada T6G 2E9