BRUGIA EST COUNT TOPS 4000
thanks mostly to the herculean efforts of the Williams lab, aided and
abetted by those of Ramzy, Slatko, Jayaraman, Blaxter, Scott and Egwang,
the total number of Brugia malayi ESTs in dbest has this morning (4/10/96)
passed the 4000 mark (actually 4023).
In early 1995, there were 94 genbank DNA entries for Brugia, corresponding
to about 60 different genes from both B. pahangi and B. malayi. Since then
the WHO sponsored genome project has been performing single-pass sequencing
on cDNA clones in a concerted gene discovery effort. The 4000 dbest entries
correspond to something in the region of 2,500 different genes: this is a
4100% increase in only 18 months of global effort. 2,500 genes corresponds
to perhaps one fifth of the total number of protein coding genes expected
in the Brugia genome.
In the dbest dataset are genes expressed in microfilariae, infective L3s,
L4s and adult males. These will soon be joined by cDNA sequences from L2
and adult female libraries.
The Williams lab has also been submitting Onchocerca volvulus ESTs to
dbest, and there are a total of 306 currently deposited.
All the data is publically available through dbest and genbank, and a guide
to searching the dbest dataset for the "clone of your dreams" has been
posted on the world wide web at URL:
You can also use the parasite-genome BLAST server to perform searches
specifically of the Brugia DNA dataset. Go to URL:
The Brugia genome labs and the Parasite-Genome centre at the EBI will
continue to develop tools for access and exploration of this data. If you
have any suggestions for this please contact me.
Secretary to the Filarial Genome Project