>> To whom it may concern,
>> Can anybody help me out with a protocol on how to prepare a culture of
> Trichomanas vaginalis for storage in liquid N2? I assume, the methods
> are different from those used in bacteria and mammalian cells. Any
> suggestions are greatly appreciated!
>> thanks in advance, sincerely,
Check this reference:
Diamond LS, Bartgis IL, and Reardon LV. 1965. Virulence of Trichomonas
vaginalis after freeze-preservation for two years in liquid nitrogen
vapor. Cryobiology 1(4):295-297.