>> Dear Colleagues,
>> If there any risk of amplifying a fragment DNA with primer which are 50 to 60 pb. Actually, I want to introduce a enzyme restriction site in these primers so that I can easily detected them in agore or Acrylamide gel. Therefore I do need longer primers. But I wandering about doing a PCR such long oligonucleotides.
> My regards,
Wellcome Trust Research Labs,
PO Box 43640,
Tel 254 (2) 725390, Fax 254 (2) 711673
E-mail: wellcome at users.africaonline.co.ke