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PST Course Announcement

Bratislav Stankovic stankovic.1 at osu.edu
Sat Sep 21 19:57:24 EST 1996


MAY 3-10, 1997

  The Course
'Single cell techniques in signal transduction research' comprises a rapidly
expanding field with an increasing number of possibilities and technical
aspects. The course provides insight in a variety of powerful single cell
techniques and attempts to give an overview of new developments in the
field. In doing so, the course is suitable for both specialist 'single cell
researchers' and for researchers from outside the 'single cell field'. In
signal transduction research, combination of e.g. biochemistry/molecular
biology with single cell techniques provides a powerful way to study
mechanisms and explore new directions.
The course consists of  lectures and practical/theoretical workshops in
small groups:
        1. Lectures, explaining how a technique works, what the problems and
limitations are etc., illustrated with research examples.
        2. Theoretical workshops discussing aspects of  different
techniques in detail.
        3. Practical workshops with hands on experiments.

       Looking forward to seeing you in the Netherlands!

The organizing committee

  Topics and Lecturers

W.F. Boron (USA):       Regulation and measurement of cytoplasmic pH.
P.H.G.M. Willems (NL):  Regulation of cytoplasmic calcium.
M. Fricker (UK):                Introduction to ion imaging. Confocal
imaging: from cells to tissue.
R.W. Horobin (UK):        Fluorescent dyes: where do they go and why?

E.L. Boulpaep (USA):            Introduction to ion transport and patch-clamp.
A.M.J. van Dongen (USA):        Ion channels and their regulation.
D. Colquhoun (UK):              Analysis of ion channel recordings.
M. Mazzanti (IT):               Ion channels in the nucleus and
special voltage clamp protocols.
S.M. Assmann (USA):              Regulation of ion channels in plant cells.

Other Techniques
W.J. Wadman (NL):                          Introduction to single cell research
C.J. Weijer (UK):                          Cell tracking.
S. Damjanovich (HUN):   Atomic force microscopy.
K.O. Greulich (GER):                       Application of lasers in single
cell research.
H.C. Gerritsen (NL):    Fluorescence life-time imaging measurements.
A.J. Trewavas (UK):     Molecular biology and calcium measurements.
R.Y. Tsien (USA):       Looking into the future of single cell research.

Theoretical Workshops

Recording of electrophysiological signals
Advanced patch-clamp
Analysis of single channel data/stochastic channel behaviour
Molecular biology of ion channels
Patch clamp on plant cell protoplasts
Photomultipliers and cameras for ion imaging measurements
Calibration and measurement of pH signals
Energy transfer measurements
Application of lasers

Practical Workshops

Patch-clamp circuitry, recording of signals
Patch clamp measurements
Patch-clamp analysis
Fluorescence measurements/imaging
Confocal microscopy
Analysis of imaging data/ calibration
Fluorescent dyes; where do they go
Cell tracking
Atomic force microscopy
Laser micro surgery

  Location  and Time Schedule

The course will be held from May 3 - May 10, 1997 in the magnificent
seventeenth century estate "Oud Poelgeest" near Leiden. Some of the
practical workshops will be held on different locations in Leiden and

Course Fee and Grants

The course fee is DM 1150,-. The fee includes registration, all meals, tea
and coffee, excursions, accommodation on basis of a double room, course book.
A limited number of FEBS Youth Travel Grants covering fee and (part of )
travel expenses are available for members of FEBS constituent societies
under the age of 31.
        Some other grants may be available through the Single Cell Research
Foundation (see application form).

Abstracts and Posters

Participants are asked to submit an abstract on their work and to present a
poster during the course. Some of the abstracts will be selected for short
oral presentations.

 Social program

An attractive social program including visits to the flower fields and Dutch
lakes will be organized.


        64 participants will be admitted.
        The organisers select participants on basis of their application.
        For application please send as soon as possible to the address below:
                 The completed application form
                A short curriculum vitae, current research interest
description and motivation to join this course (maximal 1 page)
                A letter of recommendation by supervisor or head of
department for students

The deadline for application is February 15, 1997
Applicants will be notified about their admission before March 1, 1997.

 Organising committee
        A. Wiltink, M.T. Flikweert, A.H. de Boer, S. Damjanovich,
        S. Heimovaara-Dijkstra, B. Van Duijn

 Further information and application
        Dr. Bert Van Duijn,
        Center for Phytotechnology RUL/TNO, Wassenaarseweg 64,
        2333 AL Leiden,  The Netherlands.
        Tel. +31-71-5274923,  Fax +31-71-5274863
        e-mail: FEBS at AMC.UVA.NL
        Webpage http://wwwbio.leidenuniv.nl/~imp/febs1.html

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