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postdoc announcements

Steve Huber steve_huber at ncsu.edu
Tue Sep 12 14:20:10 EST 2000


Postdoctoral Position
USDA-ARS, NC State University, Raleigh, NC
A position is available for 3 years to study the regulation of binding of
14-3-3 proteins to target polypeptides, such as phosphorylated nitrate
reductase. Divalent cations, protons and polyamines activate binding of
14-3-3s to polypeptide ligands. We will use directed mutagenesis and
C-terminal truncations to identity the cation-binding site(s), and to
determine whether specific histidine residues mediate the proton activation.
We will also characterize UDPglucose pyrophosphorylase as a new binding
partner for 14-3-3s.
Minimum requirements include a Ph.D. in a plant biology related field and a
working knowledge of biochemistry, plant physiology and molecular biology.
Experience with manipulation of cDNAs and expression of recombinant proteins
is essential. Experience with protein purification, enzyme assays and
protein phosphorylation is highly desirable. Information about our program
is available at <http://www.cropsci.ncsu.edu/faculty/huber/default.htm>.
Send a CV and names (with e-mail addresses) of 3 references to: Dr. Steven
C. Huber, USDA Plant Science Research, NC State University, Raleigh, NC
27695-7631; telephone 919-515-3906; fax 919-856-4598, email
steve_huber at ncsu.edu

***************************

Postdoctoral Position
USDA-ARS, NC State University, Raleigh, NC
A position is available for 2 years to study the control of storage product
accumulation during soybean seed development. The overall objective is to
identify the transcriptional and post-translational mechanisms that allow a
developing seed to adjust oil and storage protein biosynthesis to cope with
changes in the absolute and relative supplies of sucrose and amino acids
provided by the maternal plant for growth and development. Approaches will
include determining the effect of assimilate supply (sucrose versus amino
acids) on the expression of genes encoding lipid biosynthetic enzymes and
storage proteins to test the hypothesis that nitrogenous signals (possibly
glutamine) regulate the capacity for oil biosynthesis. In addition, the
role of reversible protein phosphorylation as a post-translational mechanism
will be investigated in developing seeds. Minimum requirements include a
Ph.D. in a plant biology related field and a working knowledge of
biochemistry, plant physiology and molecular biology. Experience with
extraction of nucleic acids, quantitation of transcripts (by Northern
analysis), and manipulation of cDNAs is essential. Experience with protein
purification and phosphorylation is highly desirable. Send a CV and names
(with e-mail addresses) of 3 references to: Dr. Steven C. Huber, USDA Plant
Science Research, NC State University, Raleigh, NC 27695-7631; telephone
919-515-3906; fax 919-856-4598, email steve_huber at ncsu.edu.








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