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post-doc positions in auxin signaling

Jason Reed jreed at email.unc.edu
Wed Feb 6 19:54:14 EST 2002

Post-docs wanted to study light and hormone regulation of plant development
The plant hormone auxin regulates growth, differentiation, and
patterning at multiple developmental stages. A family of proteins called
Aux/IAA proteins are key mediators of auxin signaling and response. Auxin
induces expression of Aux/IAA genes, and can also regulate turnover of
Aux/IAA proteins. Aux/IAA proteins in turn regulate gene expression by
interacting with transcription factors of the ARF (Auxin Response Factor)
family. Mutations in several Arabidopsis Aux/IAA and ARF genes have
revealed that they have distinct developmental roles reflecting the
diversity of activities that auxin has. Gain-of-function mutations in any
of several Aux/IAA genes stabilize the corresponding proteins, decrease
auxin response, and also cause ectopic light responses, suggesting that
both auxin and light signal through Aux/IAA proteins. Positions are
available to study Aux/IAA and ARF functions and auxin signal transduction
pathways. Depending on expertise and interests, individuals may work on
any of several projects, including:
1) Biochemical assays of auxin- or light-regulated modification of Aux/IAA
proteins; and interactions among Aux/IAA proteins and photoreceptors,
ubiquitin ligases, or ARF transcription factors. These studies will reveal
mechanisms of auxin signal transduction, and provide tools to investigate
how auxin is perceived.
2) Characterization of extragenic modifiers of gain-of-function Aux/IAA
gene mutations, and cloning the corresponding gene(s). Such genes may
encode novel components of auxin or light signaling.
3) Gene chip studies to identify regulatory targets of Aux/IAA and ARF
proteins, and to discern regulatory interactions between auxin and light.
4) Reverse genetic strategies to determine functions of additional Aux/IAA
and ARF genes, and of genes that are regulatory targets of Aux/IAA and ARF
5) Studies of embryonic shoot patterning, focusing on a gain-of-function
mutation in IAA18 that causes aberrant cotyledon number and cotyledon
6) Comparative evolutionary studies of Aux/IAA and ARF gene function in
different plant species.
For further information, please contact Jason Reed at the address
below. Individuals with experience in molecular biology, genetics, or
biochemistry are especially encouraged to apply. Please send letter
describing research interests, curriculum vitae and names of references to:

Jason W. Reed (jreed at email.unc.edu)
University of North Carolina at Chapel Hill
Department of Biology
CB #3280, Coker Hall
Chapel Hill, NC 27599-3280
Relevant recent publications:
Reed, J. W., Elumalai, R. P., and Chory, J. 1998. Suppressors of
an Arabidopsis thaliana phyB mutation identify genes that control light
signaling and hypocotyl elongation. Genetics 148: 1295-1310.
Tian, Q., and Reed, J. W. 1999. Control of auxin-regulated root
development by the Arabidopsis thaliana SHY2/IAA3 gene. Development 126:
Reed, J. W. 1999. Phytochromes are Pr-ipatetic kinases. Current
Opinion in Plant Biology 2: 393-397.
Nagpal, P., Walker, L. M., Young, J. C., Sonawala, A., Timpte, C.,
Estelle, M., and Reed., J. W. 2000. AXR2 encodes a member of the Aux/IAA
protein family. Plant Physiology 123: 563-573.
Krall, L. and Reed, J. W. 2000. The histidine kinase-related
domain participates in phytochrome B function but is dispensable.
Proceedings of the National Academy of Sciences (USA) 97: 8169-8174.
Reed, J. W. 2001. Roles and activities of Aux/IAA proteins in
Arabidopsis. Trends in Plant Science 6: 420-425.
Tian, Q. and Reed, J. W. 2001. Molecular links between light and
auxin signaling. Journal of Plant Growth Regulation 20: 274-280.
Tian, Q. Uhlir, N. J., and Reed, J. W. 2002. SHY2/IAA3 inhibits
auxin-regulated gene expression. Plant Cell, in press (February 2002).
Liscum, E. and Reed, J. W. 2002. Genetics of ARF and Aux/IAA
proteins in plant growth and development. Plant Molecular Biology, in
Jason W. Reed
University of North Carolina at Chapel Hill
Department of Biology
CB #3280, Coker Hall
Chapel Hill, NC 27599-3280
telephone: 919-962-5699 (office)
919-962-2161 (lab)
FAX: 919-962-1625
email: jreed at email.unc.edu


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