From owner-structural-nmr@net.bio.net Fri Sep 01 23:00:00 1995 Path: biosci!PIS.BEKKOAME.OR.JP!ishidak From: ishidak@PIS.BEKKOAME.OR.JP (keisuke ishida) Newsgroups: bionet.structural-nmr Subject: help Date: 1 Sep 1995 20:15:12 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: <199509020312.MAA00514@bekkoame.bekkoame.or.jp> NNTP-Posting-Host: net.bio.net help From owner-structural-nmr@net.bio.net Fri Sep 01 23:00:00 1995 Path: biosci!PIS.BEKKOAME.OR.JP!ishidak From: ishidak@PIS.BEKKOAME.OR.JP (keisuke ishida) Newsgroups: bionet.structural-nmr Subject: help Date: 1 Sep 1995 20:15:06 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: <199509020312.MAA00520@bekkoame.bekkoame.or.jp> NNTP-Posting-Host: net.bio.net help From owner-structural-nmr@net.bio.net Sun Sep 03 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!usc!howland.reston.ans.net!news.sprintlink.net!in2.uu.net!news.tele.fi!news.funet.fi!luotsi.uku.fi!usenet From: niemitz@skanssi.uku.fi (Matthias Niemitz) Newsgroups: bionet.structural-nmr Subject: Re: spin simulation Date: 4 Sep 1995 13:17:51 GMT Organization: University of Kuopio, Finland Lines: 43 Distribution: world Message-ID: <42eu9v$nun@luotsi.uku.fi> References: <423ub7$9a3@mercury.hgmp.mrc.ac.uk> NNTP-Posting-Host: s3038.uku.fi Mime-Version: 1.0 X-Newsreader: WinVN 0.99.3 In article <423ub7$9a3@mercury.hgmp.mrc.ac.uk>, m-gradwe@nimr.mrc.ac.uk says... > >We are looking for a recent spin simulation program. Does anyone have any releva >nt >details? >Thanking you in advance >Mike Gradwell >National Institute for Medical Research Review Peter Lundberg's excellent NMR-software compilation: http://www.york.ac.uk/depts/chem/nmr/edusoft.html However most of the programs are limited to 8 spins or less. More powerful systems on IBM/PC are WinDAISY and PERCH. I don't count Acron's "Nuts" into this category because it's rather slow (see below) and can't iterate. If you are looking for speed, PERCH has by far the fastest simulator (on PC). Here is some comparision made on an 486/66: no. of indiv. Nuts WinDaisy(2.0) PERCH(1/95) spins 8 3 min 1 min 8 s 10 >3 hours 1 hour <4 min (15 min with (1 min with C2 symmetry) X-aproximation) Notice that a comparision is rather difficult because computation times are very much depending on program settings. I've calculated similar cases and tried to keep run time parameters as similar as possible too. For larger systems you should switch to UNIX based programs like NUMARIT by Kirk Marat (ftp://bloch.cchem.berkeley.edu/pub/nmr/umansim/) or wait for the PERCH simulator/iterator to be ported to UNIX (a test version is running on IBM/RS6000, additionally SGI and SUN are to be supported). PERCH also provides a very fast and powerful iteration engine which enables spectral analysis also for larger systems where an iteration algorithm based on assignments is not practicable anymore. For more information about PERCH visit http://www.uku.fi/perch.html. From owner-structural-nmr@net.bio.net Mon Sep 04 23:00:00 1995 Path: biosci!HEARTLAND.BRADLEY.EDU!dweisled From: dweisled@HEARTLAND.BRADLEY.EDU (David Weisleder) Newsgroups: bionet.structural-nmr Subject: Re: spin simulation Date: 5 Sep 1995 13:03:05 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 17 Sender: daemon@net.bio.net Distribution: world Message-ID: <9509051739.AA28480@heartland.bradley.edu> Reply-To: dweisled@heartland.bradley.edu NNTP-Posting-Host: net.bio.net I am reviewing a spin simulation program for Macintosh computers called gNMR (available from SoftShell - www.softshell.com). The manual claims simulation of up to 12 nuclei. My computer is a Quadra 840AV 32/500 (40 MHz) and the software can simulate a 10 spin system in less than 8 seconds, 12 in less than 14 secs., and the program doesn't stop there. A 14 spin system takes less than a minute (54 secs). I stopped at 16 different nuclei which exactly reproduced a spectrum for a compound - simmondsin in a few minutes (sorry I didn't check my watch because I expected a crash). The program can iterate if you have a spectrum handy. -- USDA, ARS, National Center for Agricultural Utilization Research 1815 N. University St., Peoria, IL 61604-3902 (309) 681-6304 (human) (309) 681-6303 (FAX and machine) From owner-structural-nmr@net.bio.net Mon Sep 04 23:00:00 1995 Path: biosci!agate!newsxfer.itd.umich.edu!newsfeed.internetmci.com!news.sprintlink.net!ns1.win.net!numare!lbyrnes Newsgroups: bionet.structural-nmr Message-ID: <182@numare.win.net> Reply-To: lbyrnes@numare.win.net (Lawrence Byrnes) From: lbyrnes@numare.win.net (Lawrence Byrnes) Date: Tue, 05 Sep 1995 14:45:24 GMT Subject: RF amp. needed, JEOL FX60Q magnet/parts free Lines: 27 We are looking for an RF amplifier for an ENDOR system to replace an ENI 3200L. 0-200 or 300 watts, 1 MHz or lower to at least 75 MHz. We would appreciate any "less than new price" sources or anything from a junked machines. We also have a JEOL FX60Q working magnet and some miscellaneous parts from the console (broadband with Li /D lock) we are junking for any serious Lego builders free! Thank you... Kathy iouiouiouiouiouiouiouiouiouiouuomeuomeuomeuomeuomeuomeuome Kathleen S. Gallagher | Opinions are mine, not University of New Hampshire | my employer's. Parsons Hall/UIC | Durham, NH 03824 | Go out on a limb, that's 603-862-2790 | where the fruit is... kathy.gallagher@unh.edu | Will Rogers meowmeowmeowmeowmeowmeowmeowmeowmeowmeowmeowmeowmeowmeow!! From owner-structural-nmr@net.bio.net Mon Sep 04 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!newsfeed.internetmci.com!news.sprintlink.net!in2.uu.net!newsfeed.pitt.edu!xumac.anes.pitt.edu!user From: y_xu@poplar.anes.upmc.edu (Y_Xu) Newsgroups: bionet.structural-nmr Subject: !!!postdoc position available now!!! Date: 5 Sep 1995 18:17:50 GMT Organization: University of Pittsburgh Lines: 4 Message-ID: NNTP-Posting-Host: xumac.anes.pitt.edu Postdoctoral position available immediately. Knowledge in neurobiochemistry essential, background in NMR preferred. Send CV to Prof. Yan Xu, W-1358 Biomedical Science Tower, University of Pittsburgh, Pittsburgh, PA 15261; or E-mail: y_xu@poplar.anes.upmc.edu. From owner-structural-nmr@net.bio.net Wed Sep 06 23:00:00 1995 Path: biosci!JUSTINE.UMontreal.CA!tyx From: tyx@JUSTINE.UMontreal.CA Newsgroups: bionet.structural-nmr Subject: unsubscribe Date: 7 Sep 1995 11:37:57 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 3 Sender: daemon@net.bio.net Distribution: world Message-ID: <9509071734.AA13341@gen.JUSTINE.UMontreal.CA> NNTP-Posting-Host: net.bio.net unsubscribe str-nmr tyx@JUSTINE.UMontreal.CA From owner-structural-nmr@net.bio.net Wed Sep 06 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!tank.news.pipex.net!pipex!oleane!jussieu.fr!univ-lyon1.fr!swidir.switch.ch!serra.unipi.it!giuditta!vassallo From: vassallo@cli.di.unipi.it (Fabrizio Vassallo) Newsgroups: bionet.structural-nmr Subject: music & DNA Date: 7 Sep 1995 16:41:55 GMT Organization: Dipartimento di Informatica, Universita' di Pisa Lines: 6 Distribution: world Message-ID: <42n7cj$1d6g@serra.unipi.it> NNTP-Posting-Host: giuditta.cli.di.unipi.it X-Newsreader: TIN [version 1.2 PL2] Hallo! Have anyone heard of relations betw. music & DNA? please post me Fabrizio From owner-structural-nmr@net.bio.net Wed Sep 06 23:00:00 1995 Newsgroups: bionet.molbio.proteins,bionet.cellbiol,bionet.molec-model,bionet.structural-nmr,bionet.xtallography,bionet.general,sci.bio Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!Germany.EU.net!news.dfn.de!gs.dfn.de!news.uni-bielefeld.de!techfak.uni-bielefeld.de!fuellen From: fuellen@techfak.uni-bielefeld.de (Georg Fuellen) Subject: Ribosomal Proteins <-> rRNA. How much is known ? Message-ID: Sender: fuellen@TechFak.Uni-Bielefeld.DE (Georg Fuellen) Date: Thu, 7 Sep 1995 13:50:07 GMT Nntp-Posting-Host: salbei.techfak.uni-bielefeld.de Organization: Universitaet Bielefeld, Technische Fakultaet. Followup-To: bionet.molbio.proteins Lines: 23 Xref: biosci bionet.molbio.proteins:5570 bionet.cellbiol:2859 bionet.molec-model:578 bionet.structural-nmr:746 bionet.xtallography:2010 bionet.general:16874 Hi there, How much is known about the interaction between Ribosomal Proteins and the ribosomal RNA ? Have points of contact between specific nucleotides and specific amino acids been identified ? In other words, how much detail does one know about the structure of the ribosome, beyond the standard textbook picture displaying the spatial arrangement of e.g. S1-S21, represented as spheres of different size ? [M.S.Capel et al, 1988, J.M.B. 200:65; Fig. 3-20 in Darnell, Lodish, Baltimore, Molecular Cell Biology, 2nd Ed., p.99] I eagerly await the education I'm about to get :) (Follow-Up set to bionet.molbio.proteins (so I hope)) best wishes, georg fuellen@dali.Mathematik.Uni-Bielefeld.DE, fuellen@MIT.EDU http://www.techfak.uni-bielefeld.de/techfak/persons/fuellen/georgF.html From owner-structural-nmr@net.bio.net Wed Sep 06 23:00:00 1995 Path: biosci!daresbury!nntp-trd.UNINETT.no!Norway.EU.net!EU.net!howland.reston.ans.net!tank.news.pipex.net!pipex!in2.uu.net!news.tele.fi!news.funet.fi!luotsi.uku.fi!usenet From: niemitz@skanssi.uku.fi (Matthias Niemitz) Newsgroups: bionet.structural-nmr Subject: Re: spin simulation Date: 7 Sep 1995 10:10:07 GMT Organization: University of Kuopio, Finland Lines: 37 Distribution: world Message-ID: <42mgdv$fj6@luotsi.uku.fi> References: <9509051739.AA28480@heartland.bradley.edu> NNTP-Posting-Host: s3038.uku.fi Mime-Version: 1.0 X-Newsreader: WinVN 0.99.3 In article <9509051739.AA28480@heartland.bradley.edu>, dweisled@HEARTLAND.BRADLEY.EDU says... > > > >I am reviewing a spin simulation program for Macintosh computers >called gNMR (available from SoftShell - www.softshell.com). The >manual claims simulation of up to 12 nuclei. My computer is a >Quadra 840AV 32/500 (40 MHz) and the software can simulate a >10 spin system in less than 8 seconds, 12 in less than 14 secs., >and the program doesn't stop there. A 14 spin system takes less >than a minute (54 secs). I stopped at 16 different nuclei which >exactly reproduced a spectrum for a compound - simmondsin in a >few minutes (sorry I didn't check my watch because I expected >a crash). The program can iterate if you have a spectrum handy. Obviously this program is using extensive X-approximation. The computation time then very much depends on how many different species are defined in the system. The time mentioned in my last post for the 10 spin system with X-approximation was for two different species only. If you increase this number you can reduce the computation time to your values but usually you don't have more than two or three different species. Also the number of couplings and even their size have influence. One cannot compare computation times for simulation on the basis of the number of nuclei only. regards, Matthias Niemitz This time with signature (sorry for missing it in my last post). Department of Chemistry phone: + 358 71 163241 University of Kuopio fax: + 358 71 163259 P.O.B. 1627 e-mail: Matthias.Niemitz@uku.fi FIN-70211 Kuopio, FINLAND WWW: http://www.uku.fi/perch.html From owner-structural-nmr@net.bio.net Sat Sep 09 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!math.ohio-state.edu!magnus.acs.ohio-state.edu!lerc.nasa.gov!purdue!mozo.cc.purdue.edu!not-for-mail From: eog@mace.cc.purdue.edu (Vicki Finkenstadt) Newsgroups: bionet.jobs,bionet.xtallography,sci.techniques.xtallography,misc.jobs.offered,bionet.structural-nmr Subject: POSTDOCTORAL POSITION Computational Crystallography Purdue University Date: 10 Sep 1995 11:28:21 -0500 Organization: Purdue University Lines: 33 Message-ID: <42v3n5$k08@mace.cc.purdue.edu> NNTP-Posting-Host: mace.cc.purdue.edu Xref: biosci bionet.xtallography:2016 sci.techniques.xtallography:1883 misc.jobs.offered:338921 bionet.structural-nmr:751 POSTDOCTORAL POSITION Computational Crystallography A postdoctoral position is available at Purdue University on projects concerned with the development of new computational algorithms for macromolecular crystallography. Projects include new density modification algorithms for protein crystallography, analysis of disordered polymer structures, and other theoretical and computational aspects of x-ray crystallography. Applicants should have a Ph.D. and experience in computational methods in x-ray crystallography, or in a related field. Experience with C programming and familiarity with Unix is essential. Experience with Motif or Open/GL is desirable. Send cv, a summary of research experience and interests, and the names of three referees to Dr. Rick Millane, Whistler Center for Carbohydrate Research, Computational Science and Engineering Program, Purdue University, West Lafayette, IN 47907-1160, tel. 317-494-9272, fax. 317-494-7953, ojg@mace.cc.purdue.edu. Purdue University is an equal opportunity/affirmative action employer. -- Vicki Finkenstadt eog@mace.cc.purdue.edu Carbohydrate Center vicki@kiwi.foodsci.purdue.edu Purdue University http://www.foodsci.purdue.edu/~vicki "There is much that is true, wise and beautiful in this world." From owner-structural-nmr@net.bio.net Sat Sep 09 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!vixen.cso.uiuc.edu!prairienet.org!glturner From: glturner@prairienet.org (Gary L. Turner) Newsgroups: bionet.structural-nmr Subject: Used MR Equipment Newsletter Date: 10 Sep 1995 15:37:32 GMT Organization: University of Illinois at Urbana Lines: 35 Message-ID: <42v0ns$kag@vixen.cso.uiuc.edu> NNTP-Posting-Host: firefly.prairienet.org Announcing: "The Larmor Letter", a free on-line newsletter for announcements of used NMR, MRI, and EPR equipment for sale, wanted, or for trade. This newsletter is distributed freely to all concerned, and there is no charge to list your announcement. There are no restrictions as to whom may submit announcements. Types of equipment can be spectrometers, probes, consoles, amplifiers, "spare parts", or any other equipment used in MR. To submit an announcement for MR equipment available or wanted, send a brief description of the equipment to sdsnmr@aol.com. Include the name of a contact person, and their email address and phone/fax number. Please note that The Larmor Letter will neither buy, sell, or trade equipment, or make recommendations. The Larmor Letter will not assume any liability for non-performance of any equipment listed herein. The Larmor Letter will not act as an agent in any transaction. Anyone wishing to receive this free newsletter should send a request to sdsnmr@aol.com. Comments, flames, etc. to same. ================================================================= Gary Turner Spectral Data Services, Inc. Contract NMR Data Acquisition phone : (217)352-7084 fax : (217)352-9748 email :sdsnmr@aol.com http://www.prairienet.org/~glturner/sdsnmr.html -- From owner-structural-nmr@net.bio.net Sun Sep 10 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!tank.news.pipex.net!pipex!sunsite.doc.ic.ac.uk!hgmp.mrc.ac.uk!nimr.mrc.ac.uk!m-gradwe From: m-gradwe@nimr.mrc.ac.uk (Mike Gradwell) Newsgroups: bionet.structural-nmr Subject: 3D plots from Felix Date: 11 Sep 1995 16:22:49 GMT Organization: National-Institute-for-Medical-Research Lines: 7 Distribution: world Message-ID: <431nop$hsu@mercury.hgmp.mrc.ac.uk> NNTP-Posting-Host: nimsb42.nimr.mrc.ac.uk Hello, Is there anyone out there who has written a macro for plotting Felix 3D matrices (rather than plotting slice by slice!). Failing that, is it possible to create a plotfile in XEASY containing multiple slices from a 3D? Mike Gradwell National Institute For Medical Research From owner-structural-nmr@net.bio.net Tue Sep 12 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!news.nic.surfnet.nl!ruu.nl!news From: Gerard Robijn Newsgroups: bionet.structural-nmr Subject: Bruker pulse sequence HMBC experiment Date: 13 Sep 1995 15:54:07 GMT Organization: Academic Compter Centre Utrecht Lines: 8 Message-ID: <436uqv$8qa@nic.cc.ruu.nl> NNTP-Posting-Host: ruucj5.chem.ruu.nl Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (X11; I; IRIX 5.2 IP20) X-URL: news:bionet.structural-nmr Who could help me with an HMBC experiment. Since I have never recorded an HMBC experiment before, and since I am not a real NMR expert, it would be nice if someone could give me an "almost-ready-to-go" pulse program for a Bruker AMX-500/600 machine. I am aware of the original literature on this type of experiment (Bax and Summers, J. Am. Chem. Soc., 108 (1986) 2093), but if someone knows a more clever, and more sensitive version of the experiment, I would be happy to hear about it, of course. From owner-structural-nmr@net.bio.net Tue Sep 12 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.sprintlink.net!in2.uu.net!EU.net!sun4nl!news.nic.surfnet.nl!ruu.nl!news From: Gerard Robijn Newsgroups: bionet.structural-nmr Subject: Bruker pulse sequence HMBC experiment-2 Date: 13 Sep 1995 16:00:30 GMT Organization: Academic Compter Centre Utrecht Lines: 18 Message-ID: <436v6u$9e1@nic.cc.ruu.nl> NNTP-Posting-Host: ruucj5.chem.ruu.nl Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (X11; I; IRIX 5.2 IP20) To: gerard@boc.chem.ruu.nl X-URL: news:bionet.structural-nmr Who could help me with an HMBC experiment. Since I have never recorded an HMBC experiment before, and since I am not a real NMR expert, it would be nice if someone could give me an "almost-ready-to-go" pulse program for a Bruker AMX-500/600 machine. I am aware of the original literature on this type of experiment (Bax and Summers, J. Am. Chem. Soc., 108 (1986) 2093), but if someone knows a more clever, and more sensitive version of the experiment, I would be happy to hear about it, of course. Gerard W. Robijn Bijvoet Center for biomolecular research Department of Bio-Organic Chemistry P.O. Box 80.075 Visiting address: 3508 TB Utrecht H.R. Kruyt Building, O703 The Netherlands (Transitorium III) Phone: +31 30 53 3752 Padualaan 8 Fax : +31 30 54 0980 3584 CH Utrecht e-mail: gerard@boc.chem.ruu.nl From owner-structural-nmr@net.bio.net Thu Sep 14 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!tank.news.pipex.net!pipex!dispatch.news.demon.net!demon!sunsite.doc.ic.ac.uk!daresbury!not-for-mail From: "Lennart Nilsson, KI/CSB" Newsgroups: bionet.structural-nmr Subject: ANNOUNCEMENT: Molecular Recognition and Signalling. Date: 15 Sep 1995 08:52:28 +0100 Lines: 30 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <43bbbs$km4@mserv1.dl.ac.uk> X-Mts: smtp Original-To: pdb-l@pdb.pdb.bnl.gov, bioforum@dl.ac.uk, bionews@dl.ac.uk, biophys@dl.ac.uk, bio-soft@dl.ac.uk, comp-bio@dl.ac.uk, methods@dl.ac.uk, molmodel@dl.ac.uk, proteins@dl.ac.uk, xtal-log@dl.ac.uk, str-nmr@dl.ac.uk Posted-Date: Fri, 15 Sep 95 09:51:06 +0200 MOLCULAR RECOGNITION AND SIGNALLING Joint Meeting of the British and Swedish Biophysical Societies University of Bath, 19-21 December 1995 Sessions: Specific and non-specific recognition Molecular docking and design Recognition during signalling Recognition in the immune system Signalling across membranes More information is available at http://www.csb.ki.se/events/bbs-bath95.html To receive a registration form please contact Prof. A. R. Rees School of Biology and Biochemistry University of Bath Bath BA2 7AY UK FAX: Int+44 1225 826779 e-mail: bssarr@bath.ac.uk Organising committe: Professor A. R. Rees, University of Bath Professor J. Thornton, University College, London Dr. L. Nilsson, Karolinska institutet, Stockholm From owner-structural-nmr@net.bio.net Thu Sep 14 23:00:00 1995 Path: biosci!NARCISSUS.CHEM.SUNYSB.EDU!smita From: smita@NARCISSUS.CHEM.SUNYSB.EDU (smita mohanty) Newsgroups: bionet.structural-nmr Subject: I want to be a subscriber of STRUCTURAL-NMR Date: 15 Sep 1995 11:36:12 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 17 Sender: daemon@net.bio.net Distribution: world Message-ID: <9509151838.AA01065@narcissus.chem.sunysb.edu> NNTP-Posting-Host: net.bio.net Dear Sir/ Madam, Please include me in your mailing list. I am a solution NMR chemist working at the chemistry department, State Univ of New York at Stony Brook. Please let me know if I need to anything else to be a subsciber of Str-nmr. Thank you very much. Smita Mohanty, Ph.D Chemistry Department State University Of New York Stony Brook Stony Brook, Ny -11720-3400 From owner-structural-nmr@net.bio.net Thu Sep 14 23:00:00 1995 Path: biosci!PASTEUR.FR!murield From: murield@PASTEUR.FR (Muriel Delepierre) Newsgroups: bionet.structural-nmr Subject: Pst-doc wanted Date: 14 Sep 1995 17:50:25 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 22 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net We are seeking an NMR spectroscopist to join the Pasteur Institute NMR laboratory. We are looking for an individual with expertise in methodology and particularly 3D, hetero, homo, shaped pulse, pulse field gradients.... as applied to biological molecules. Our laboratory is equipped with a three channels 500 MHz (Varian Unity) with PFG and several probes. The Pasteur Institute is located in the center of Paris and is a very dynamic place to work. Further informations can be obtained through the net or by mail Dr Muriel DELEPIERRE Muriel DELEPIERRE Tel (1) 45 68 88 71; 45 68 88 70 Laboratoire RMN Fax (1) 45 68 88 85 Institut Pasteur 28 rue du Dr Roux 75724 PARIS cedex 15 FRANCE From owner-structural-nmr@net.bio.net Sun Sep 17 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!inet-nntp-gw-1.us.oracle.com!nntp-hub.barrnet.net!varian.com !news.nmr.varian.com!xmac.al.nmr.varian.com!user From: slp@nmr.varian.com (Steve Patt) Newsgroups: bionet.structural-nmr,sci.techniques.mag-resonance,ba.jobs.offered,comp.jobs,us.jobs.offered Subject: RESEARCH INTERN-ADVANCED INSTRUMENTATION DEVELOPMENT, Palo Alto, California Date: Mon, 18 Sep 1995 16:09:06 -0800 Organization: Varian NMR Instruments Lines: 27 Message-ID: NNTP-Posting-Host: xmac.al.nmr.varian.com Xref: biosci bionet.structural-nmr:758 sci.techniques.mag-resonance:1200 ba.jobs.offered:119261 Varian Associates, Inc. has an opportunity for a Research Intern in its E. L. Ginzton Research Center, Measurements Laboratory, for work on advanced instrumentation development in support of NMR products. This is a full-time research appointment within the Research Center for a period of two years. We are looking for an individual to participate in research and development on analytical NMR systems in the areas of digital signal processing implementation, and application or adaptation of advanced software programming (e.g., fuzzy logic or neural network approaches.) Project activities will address instrument system control, real-time sample handling, and data acquisition and processing. The successful candidate will work closely with business unit development scientists and engineers in the conception and realization of designs. Prerequisites include a Ph.D. in Physical Chemistry, Physics or Electronic Engineering and a record of experience with advanced instrumentation system development relevant to this area. Excellent verbal and written communications skills, and strong interpersonal relations skills are essential. The ability to function productively and efficiently in a fast-paced team-oriented environment is a key feature of this R&D position. For immediate consideration forward your resume to: Varian Associates, Inc., M/S E154-PD, 3050 Hansen Way, Palo Alto, CA 94304 or E-mail to britt.sellin@whq.varian.com. Varian is an equal opportunity employer and provides a drug-and-smoke-free environment. From owner-structural-nmr@net.bio.net Tue Sep 19 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!vixen.cso.uiuc.edu!newsrelay.iastate.edu!newsxfer.itd.umich.edu!nntp.cs.ubc.ca!unixg.ubc.ca!news.bc.net!news.sfu.ca!ian From: gay@sfu.ca (Ian Gay) Newsgroups: bionet.structural-nmr Subject: Re: NMR TUBES Date: Wed, 20 Sep 95 17:44:12 GMT Organization: Simon Fraser University Lines: 16 Message-ID: <43pgdt$t5h@morgoth.sfu.ca> References: NNTP-Posting-Host: ian.chem.sfu.ca X-Newsreader: News Xpress Version 1.0 Beta #4 In article , mjh2@MOLE.BIO.CAM.AC.UK ("Mark J Howard ", Biochemistry) wrote: > >Dear Netters, > >I have just been stumped by a question from a friend in the UK. Perhaps >some of you may know the answer: > >Does anyone know where you can purchase NMR tubes which do not contain >boron in the glass. Apparently both GOSS and Aldrich only have tubes >with boron in. > >Thanks > Wilmad sell fused quartz tubes. (At about 5x the price of glass ones) From owner-structural-nmr@net.bio.net Tue Sep 19 23:00:00 1995 Path: biosci!MAILBOX.SYR.EDU!ipelczer From: ipelczer@MAILBOX.SYR.EDU (Istvan Pelczer) Newsgroups: bionet.structural-nmr Subject: Bruker model/INAPT Date: 20 Sep 1995 04:06:08 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 20 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I had no intention to invent a new Bruker model in my previous message about the INAPT sequence: the DMS should be understood as DMX... (sorry for the typho) Cheers, Istvan =========================================================================== Istvan Pelczer, Ph.D. (ipelczer@mailbox.syr.edu) Res. Assist. Professor Visiting Assist. Professor Chemistry Department, CST Bldg. SUNY ESF, Chemistry Department Syracuse University One Forestry Drive Syracuse, NY 13244-4100 Syracuse, NY 13210-2778 ph: (315) 443 1023 or x-5932 ph# (315) 470 6596 or x-6855(Dept.) fax: x-1022 or x-4070(Dept.) From owner-structural-nmr@net.bio.net Tue Sep 19 23:00:00 1995 Path: biosci!MOLE.BIO.CAM.AC.UK!mjh2 From: mjh2@MOLE.BIO.CAM.AC.UK ("Mark J Howard ", Biochemistry) Newsgroups: bionet.structural-nmr Subject: NMR TUBES Date: 20 Sep 1995 03:49:06 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 18 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear Netters, I have just been stumped by a question from a friend in the UK. Perhaps some of you may know the answer: Does anyone know where you can purchase NMR tubes which do not contain boron in the glass. Apparently both GOSS and Aldrich only have tubes with boron in. Thanks Mark Howard mjh2@mole.bio.cam.ac.uk From owner-structural-nmr@net.bio.net Tue Sep 19 23:00:00 1995 Path: biosci!MAILBOX.SYR.EDU!ipelczer From: ipelczer@MAILBOX.SYR.EDU (Istvan Pelczer) Newsgroups: bionet.structural-nmr Subject: Re: Bruker pulse sequence HMBC experiment-2 Date: 19 Sep 1995 07:22:38 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 102 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <436v6u$9e1@nic.cc.ruu.nl> I guess the pulse program library of your NMR does have something for such an experiment. Please, be advised that there is an advanced data processing option which retains higher resolution (the same as that for absorption lineshape) in one dimension from Bax & Marion [JMR 78(1988)186-191]. Let me insert here a pulse sequence for selective-INEPT, which can be a sensitive alternative to HMBC and works very nicely even with moderate dispersion of the signals in the 1H dimension. It is a Bruker DMS version, but I guess it will not be difficult to convert to AMX if necessary. Good luck, Istvan =========================================================================== Istvan Pelczer, Ph.D. (ipelczer@mailbox.syr.edu) Res. Assist. Professor Visiting Assist. Professor Chemistry Department, CST Bldg. SUNY ESF, Chemistry Department Syracuse University One Forestry Drive Syracuse, NY 13244-4100 Syracuse, NY 13210-2778 ph: (315) 443 1023 or x-5932 ph# (315) 470 6596 or x-6855(Dept.) fax: x-1022 or x-4070(Dept.) On 13 Sep 1995, Gerard Robijn wrote: > Who could help me with an HMBC experiment. Since I have never recorded an HMBC > experiment before, and since I am not a real NMR expert, it would be nice if > someone could give me an "almost-ready-to-go" pulse program for a Bruker > AMX-500/600 machine. I am aware of the original literature on this type of > experiment (Bax and Summers, J. Am. Chem. Soc., 108 (1986) 2093), but if > someone knows a more clever, and more sensitive version of the experiment, I > would be happy to hear about it, of course. > > Gerard W. Robijn > Bijvoet Center for biomolecular research > Department of Bio-Organic Chemistry > P.O. Box 80.075 Visiting address: > 3508 TB Utrecht H.R. Kruyt Building, O703 > The Netherlands (Transitorium III) > Phone: +31 30 53 3752 Padualaan 8 > Fax : +31 30 54 0980 3584 CH Utrecht > e-mail: gerard@boc.chem.ruu.nl > ********************************** the pulse sequence: ; ; inapt_i ; ; selective INEPT, according to: ; Bax, A.; JMR 57(1984)314-318 ; ; Istvan Pelczer 8/10/95 (ipelczer@mailbox.syr.edu) ; d5=(p1-p10)/2 d6=(p1-p10) 1 ze 20u pl1:f1 ; set power for carbon channel 2 d10 ; additional relax time 3 d11 ; for the loop only [30 ms] d12 pl2:f2 ; set low power for 1H pulsing (p1 ph1):f2 ; soft 90 1H [ca. 10 ms] d1 ; ca. 1/(4*J[lr]) -- ca. 25 ms (d5 p10*2 ph10 d5) (p1*2 ph2):f2 ; simultaneous 180 pulses d1 (d6 p10 ph11) (p1 ph3):f2 ; simultaneous 90 pulses d2 ; ca. 1/(4*J[lr]) -- ca. 25 ms (d5 p10*2 ph12 d5) (p1*2 ph4):f2 ; simultaneous 180 pulses d2 pl3:f2 ; set power for decoupling go=2 ph5 cpd:f2 ; acquisition d10 wr #0 ; write file on disk lo to 3 times l1 ; acquire NS*l1 scans exit ph1 = 0 ph2 = 1 ph3 = 1 3 ph4 = 0 ph10 = 0 0 2 2 1 1 3 3 ph11 = 0 0 2 2 1 1 3 3 ph12 = 0 0 2 2 1 1 3 3 ph5 = 0 2 2 0 1 3 3 1 ; proton soft pulse should cover ca. 25 Hz ; d1, d2 = delays for the INAPT transfer -- other than CH may need ; different values (see paper) ; d5, d6 = compensation delays for different pulse widths ; p1, p10 = 90 deg pulses for 1H, 13C, respectively ; NS = 2 * n ; f2 should be on top of the 1H peak selected From owner-structural-nmr@net.bio.net Tue Sep 19 23:00:00 1995 Path: biosci!MAILBOX.SYR.EDU!ipelczer From: ipelczer@MAILBOX.SYR.EDU (Istvan Pelczer) Newsgroups: bionet.structural-nmr Subject: Re: tar problem (Bruker) Date: 20 Sep 1995 13:12:37 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 56 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net Have you tried the "dd" command? In our machines (Sun, SGI) it used to be a help sometimes. Take a look at the manpages, too, but probably the following will work: dd if=/dev/rmt0.1 | tar xvfo - Maybe someone else with more experience can give some better advise if this would not work. Good luck, Istvan =========================================================================== Istvan Pelczer, Ph.D. (ipelczer@mailbox.syr.edu) Res. Assist. Professor Visiting Assist. Professor Chemistry Department, CST Bldg. SUNY ESF, Chemistry Department Syracuse University One Forestry Drive Syracuse, NY 13244-4100 Syracuse, NY 13210-2778 ph: (315) 443 1023 or x-5932 ph# (315) 470 6596 or x-6855(Dept.) fax: x-1022 or x-4070(Dept.) On Wed, 20 Sep 1995, M.A. Cremonini wrote: > Dear collegues, > I have recorded on tape (by using the mxtar command on amx600) several > NMR experimental data (FIDs and Spectra). Unfortunately when back to my > risc rs6000 station I was not able to recover the files (using the > normal tar xvf /dev/rmt0.1 command). Apparently my > streamer does not detect where the last file on tape ends and so it > cannot end the session. This means that if another session is present on > tape it is simply ignored. > Has anybody had a similar problem while reading tapes recorded with the > mxtar command? Can anybody help me? > > Thank you very much. > Mauro > > P.S. > Of course, the amx600 streamer reads the same tape very well!! > ^^^^ > > ============================================ > Mauro A. Cremonini, Ph.D. > Institute of Agricultural Chemistry > University - Bologna - Italy > e-mail: m.a.cremonini@nmrlab.ciam.unibo.it > ============================================ > > > > From owner-structural-nmr@net.bio.net Tue Sep 19 23:00:00 1995 Path: biosci!HELIX.NIH.GOV!barchi From: barchi@HELIX.NIH.GOV (Joe Barchi) Newsgroups: bionet.structural-nmr Subject: mxtar problems Date: 20 Sep 1995 14:25:32 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 37 Sender: daemon@net.bio.net Distribution: world Message-ID: <199509202123.RAA29646@helix.nih.gov> NNTP-Posting-Host: net.bio.net Mauro A. Cremonini wrote: Dear collegues, I have recorded on tape (by using the mxtar command on amx600) several NMR experimental data (FIDs and Spectra). Unfortunately when back to my risc rs6000 station I was not able to recover the files (using the normal tar xvf /dev/rmt0.1 command). Apparently my streamer does not detect where the last file on tape ends and so it cannot end the session. This means that if another session is present on tape it is simply ignored. Has anybody had a similar problem while reading tapes recorded with the mxtar command? Can anybody help me? Thank you very much. Mauro P.S. Of course, the amx600 streamer reads the same tape very well!! I think many have had the same problem in trying to transfer data stored with mxtar. I believe you must use tar if you want to port data from the AMX to the workstation since mxtar is used for appending data at the end of a tape which already contains tar-ed files. This also requires that you store data in one step, hence the datasets on the AMX must have similar names or extensions where wild cards may be used. If you use "tar" on both tape drives you should be able to recover the data on the rs6000. Buona Fortuna, Joe Barchi NIH/NCI Bldg 37, Rm 5C02 Bethesda, MD 20892 barchi@helix.nih.gov From owner-structural-nmr@net.bio.net Tue Sep 19 23:00:00 1995 Path: biosci!rutgers!gatech!news.mathworks.com!tank.news.pipex.net!pipex!usenet.eel.ufl.edu!noc.netcom.net!news.sprintlink.net!simtel!swidir.switch.ch!in2p3.fr!univ-lyon1.fr!dsi.unimi.it!sirio.cineca.it!nmrlab.ciam.unibo.it!mauro From: "M.A. Cremonini" Newsgroups: bionet.structural-nmr,sci.techniques.mag-resonance Subject: tar problem (Bruker) Date: Wed, 20 Sep 1995 19:58:01 +0100 Organization: Cineca Lines: 26 Message-ID: NNTP-Posting-Host: nmrlab.ciam.unibo.it Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Xref: biosci bionet.structural-nmr:763 sci.techniques.mag-resonance:1204 Dear collegues, I have recorded on tape (by using the mxtar command on amx600) several NMR experimental data (FIDs and Spectra). Unfortunately when back to my risc rs6000 station I was not able to recover the files (using the normal tar xvf /dev/rmt0.1 command). Apparently my streamer does not detect where the last file on tape ends and so it cannot end the session. This means that if another session is present on tape it is simply ignored. Has anybody had a similar problem while reading tapes recorded with the mxtar command? Can anybody help me? Thank you very much. Mauro P.S. Of course, the amx600 streamer reads the same tape very well!! ^^^^ ============================================ Mauro A. Cremonini, Ph.D. Institute of Agricultural Chemistry University - Bologna - Italy e-mail: m.a.cremonini@nmrlab.ciam.unibo.it ============================================ From owner-structural-nmr@net.bio.net Wed Sep 20 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!uunet!in2.uu.net!news.iij.ad.jp!sumitomo-chem.co.jp!news From: tuokada@ohsun01.sumitomo-chem.co.jp (Akihiko Okada( =?ISO-2022-JP?B?GyRCMixFRCEhTEBJJxsoQg==?= )) Newsgroups: bionet.structural-nmr Subject: Re: Bruker pulse sequence HMBC experiment-2 Date: 21 Sep 1995 00:51:51 GMT Organization: Sumitomo Chemical Co. Lines: 8 Message-ID: <43qcv7$s8b@ohsun01.sumitomo-chem.co.jp> References: <436v6u$9e1@nic.cc.ruu.nl> NNTP-Posting-Host: tupc23.sumitomo-chem.co.jp Mime-Version: 1.0 X-Newsreader: WinVN 0.99.3J You'll find the pulse-program named "inv4lplrnd" in the /u/exp/stan/nmr/lists/pp/ directory. That's it. If you want to have an absorption-mode line shape in F1(13C?) dimension, you have to modify it. - Akihiko Okada tuokada@ohsun01.sumitomo-chem.co.jp From owner-structural-nmr@net.bio.net Wed Sep 20 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!news.sprintlink.net!in2.uu.net!EU.net!uknet!bhamcs!news.ox.ac.uk!news From: Isabelle Phan Newsgroups: bionet.molbio.proteins,bionet.structural-nmr Subject: ANNOUNCE: Protein structures WEB Date: 21 Sep 1995 11:42:04 GMT Organization: Oxford University Lines: 22 Message-ID: <43rj2c$60g@news.ox.ac.uk> References: <43mcha$799@news.ox.ac.uk> NNTP-Posting-Host: nmrx.ocms.ox.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (X11; I; SunOS 4.1.3 sun4c) X-URL: news:43mcha$799@news.ox.ac.uk Xref: biosci bionet.molbio.proteins:5694 bionet.structural-nmr:768 Dear All, Protein NMR structures determined by the ID Campbell group are accessible at http://www.ocms.ox.ac.uk/idc/ Coordinates, reference, abstract and pretty pictures are available for over 20 proteins. Includes links to the CYTOKINES pages and much more at the Oxford Centre for Molecular Sciences. Regards Isabelle ---------------------------------------------------------- Isabelle Phan Dept. of Biochemistry voice: 44-1865-275773 South Parks Road fax: 44-1865-275253 OXFORD OX1 3QU, U.K. email: phan@bioch.ox.ac.uk ---------------------------------------------------------- From owner-structural-nmr@net.bio.net Wed Sep 20 23:00:00 1995 Path: biosci!CHEM.PURDUE.EDU!santini From: santini@CHEM.PURDUE.EDU (Robert Santini) Newsgroups: bionet.structural-nmr Subject: Low Boron Background NMR Date: 21 Sep 1995 14:37:20 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 50 Sender: daemon@net.bio.net Distribution: world Message-ID: <199509212137.OAA15886@net.bio.net> NNTP-Posting-Host: net.bio.net >Mark Howard wrote to the net as follows: >Does anyone know where you can purchase NMR tubes which do not contain boron in the glass. Apparently both GOSS and Aldrich only have tubes with boron in. >Gary Turner replied: >Wilmad Glass sells quartz tubes, which are excellent for boron NMR. I am answering to the net as the problem may affect other NMR labs. I am curious why this is a problem to Bio-NMR people however | - ). We encountered this phenomenon many years ago when H. C. Brown was setting up an FT-80 for B-11 work. At that time, the use of quartz NMR tubes from Wilmad were a good solution. However, a few years ago we replaced the FT-80 with a 300 MHz system. The B-11 background effects reappeared with a vengeance, even with quartz tubes. The source of the signal was the Pyrex glass dewar structure in the NMR probe. Pyrex is about 14% borate so this is not surprising. The signal was strong enough to clip the receiver, but its relaxation time was very short. Most users did not notice the signal directly, but complained of "phasing difficulties" in their high resolution data. The original vendor supplied a rebuilt probe with a quartz dewar. Later we purchased a boron free Quad-Nucleus probe from an after-market vendor. The complete removal of the Pyrex was the only practical solution. Digital games with the raw data were only partially successful in the presence of the background signal. It was possible to demonstrate that the probe background signal was much stronger than that from the NMR tube due to the close coupling of the dewar to the receiver coil. There was one other side-effect of using quartz. Its AL-27 background is intense. This was not a factor in our case, but it might be important to another lab. The original FT-80 probe was fabricated with a quartz insert structure, thus the quartz tube was sufficient. --Bob Santini Robert E. Santini Department of Chemistry Purdue University W. Lafayette, IN 47907-1393 santini@chem.purdue.edu Voice: (317) 494-5227 Facsimile: (317) 494-0239 From owner-structural-nmr@net.bio.net Wed Sep 20 23:00:00 1995 Path: biosci!daresbury!nntp-trd.UNINETT.no!Norway.EU.net!EU.net!howland.reston.ans.net!swrinde!sdd.hp.com!vixen.cso.uiuc.edu!prairienet.org!glturner From: glturner@prairienet.org (Gary L. Turner) Newsgroups: bionet.structural-nmr Subject: Re: NMR TUBES Date: 21 Sep 1995 03:49:02 GMT Organization: University of Illinois at Urbana Lines: 28 Message-ID: <43qnbe$f7s@vixen.cso.uiuc.edu> NNTP-Posting-Host: firefly.prairienet.org >Dear Netters, > >I have just been stumped by a question from a friend in the UK. Perhaps >some of you may know the answer: > >Does anyone know where you can purchase NMR tubes which do not contain >boron in the glass. Apparently both GOSS and Aldrich only have tubes >with boron in. > >Thanks > > >Mark Howard > >mjh2@mole.bio.cam.ac.uk Wilmad Glass sells quartz tubes, which are excellent for boron NMR. Gary ************************************************************************ Gary Turner Spectral Data Services, Inc. Contract NMR Data Acquisition phone : (217)352-7084 fax : (217)352-9748 email :sdsnmr@aol.com http://www.prairienet.org/~glturner/sdsnmr.html -- From owner-structural-nmr@net.bio.net Thu Sep 21 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!gatech!news.mathworks.com!news.kei.com!simtel!swidir.switch.ch!in2p3.fr!univ-lyon1.fr!dsi.unimi.it!sirio.cineca.it!nmrlab.ciam.unibo.it!mauro From: "M.A. Cremonini" Newsgroups: bionet.structural-nmr,sci.techniques.mag-resonance Subject: my mxtar (bruker) problem fixed! Date: Fri, 22 Sep 1995 09:42:52 +0100 Organization: Cineca Lines: 47 Message-ID: NNTP-Posting-Host: nmrlab.ciam.unibo.it Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Xref: biosci bionet.structural-nmr:770 sci.techniques.mag-resonance:1211 Dear Collegues, since I placed a question here on the news I received several answers. My problem concerned the reading of a tape recorded with the mxtar command on the amx600 bruker nmr. I forward here the answer coming from Bruker and hope it can help other folks having the same problem. Thank you all again. Mauro ============================================ Mauro A. Cremonini, Ph.D. Institute of Agricultural Chemistry University - Bologna - Italy e-mail: m.a.cremonini@nmrlab.ciam.unibo.it ============================================ ------------------ forwarded message ------------------------- The first advice is to read the man-pages (yes, they exist) for tar + mxtar on the ASPECT X32 system. The basic concept is that 'mxtar' simulates a combination of 'mt' and 'tar' commands which you may reproduce, although less conveniently, on other computers. To retrieve session 2 of an 'mxtar' tape, you issue the following commands on your machine: mt -f rewind # rewind mt -f fsf 2 # skip 2 file marks, forwards tar vxf # extract session 2 with describing the non-rewind variant of your tape device driver. Using the 'rewind' variant defeats any effect of the mt program; you would always see session 0. ============================================ ur@spectrospin.ch Ulrich Roos SPECTROSPIN AG Industriestrasse 26 CH-8117 Faellanden ============================================ ------------------------- end of forwarded message ------------------- From owner-structural-nmr@net.bio.net Fri Sep 22 23:00:00 1995 Path: biosci!BIOC01.UTHSCSA.EDU!raman From: raman@BIOC01.UTHSCSA.EDU (C.S.RAMAN) Newsgroups: bionet.structural-nmr Subject: problem posting Date: 23 Sep 1995 13:15:49 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 39 Sender: daemon@net.bio.net Distribution: world Message-ID: <9509232013.AA02985@bioc01.uthscsa.edu> NNTP-Posting-Host: net.bio.net To all readers of the STR-NMR newsgroup: I have sent email to both the person who sent this posting and the internet provider who has issued an account to him regarding invasion of privacy and contribution of irrelevant material. I hope this doesn't happen again, but, trying to prevent such posts is quite difficult. Anyone with access to the newsgroup's email address can post without having to be a subscriber. The best thing to do, under these circumstances is to use the DELETE key to our advantage and not waste our precious time in dealing with these morons. If you have other suggestions, please send them directly to me, rather than posting it to the entire net and wasting bandwidth. Thank you very much for your cooperation in this matter. Cheers -raman -- _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/ _/ _/ _/ C.S.RAMAN _/ _/ Department of Biochemistry _/ _/ University of Texas Health Science Center _/ _/ 7703 Floyd Curl Drive _/ _/ San Antonio, TX 78284-7760 _/ _/ USA _/ _/ _/ _/ Tel: (210) 567-6623 _/ _/ Fax: (210) 567-6595 _/ _/ E-mail: raman@bioc01.uthscsa.edu _/ _/ _/ _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/ _/ _/ _/ How can it be that mathematics, a product of human thought _/ _/ independent of experience, is so admirably adapted to the _/ _/ objects of reality? -Albert Einstein _/ _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/ From owner-structural-nmr@net.bio.net Fri Sep 22 23:00:00 1995 Path: biosci!MAGNET.FSU.EDU!vincent From: vincent@MAGNET.FSU.EDU (Sebastien Vincent) Newsgroups: bionet.structural-nmr Subject: Re: WINDOWS "95 VIDEO TRAINING Date: 23 Sep 1995 12:20:03 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 4 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net >Why are you advertising on this newgroup. Es ist verboten!! Do we spam? From owner-structural-nmr@net.bio.net Fri Sep 22 23:00:00 1995 Path: biosci!CVX12.INET.DKFZ-HEIDELBERG.DE!dok730 From: dok730@CVX12.INET.DKFZ-HEIDELBERG.DE (Karl Jalkanen) Newsgroups: bionet.structural-nmr Subject: WINDOWS "95 VIDEO TRAINING Date: 23 Sep 1995 11:30:45 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <199509231515.RAA23732@cvx12.inet.dkfz-heidelberg.de> References: <43vsqu$mjc@newsie.wis.com> NNTP-Posting-Host: net.bio.net Why are you advertising on this newgroup. Es ist verboten!! From owner-structural-nmr@net.bio.net Fri Sep 22 23:00:00 1995 Path: biosci!CVX12.INET.DKFZ-HEIDELBERG.DE!dok730 From: dok730@CVX12.INET.DKFZ-HEIDELBERG.DE (Karl Jalkanen) Newsgroups: bionet.structural-nmr Subject: [MAILER-DAEMON@ns.DKFZ-Heidelberg.de: Returned mail: Host unknown (Name server: videotraining.com: host not found)] Date: 23 Sep 1995 08:45:12 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 22 Sender: daemon@net.bio.net Distribution: world Message-ID: <199509231543.RAA23928@cvx12.inet.dkfz-heidelberg.de> NNTP-Posting-Host: net.bio.net The post WINDOWS 95 Video training seems to have been posted to our group and the return address seems to be wrong. We should add this host to our system kill file. Is that possible so we do not get any more junk e-mail from this address. Date: Sat, 23 Sep 1995 17:34:24 +0200 Subject: Returned mail: Host unknown (Name server: videotraining.com: host not found) MIME-Version: 1.0 This is a MIME-encapsulated message ----- The following addresses had delivery problems ----- (unrecoverable error) ----- Transcript of session follows ----- 550 ... Host unknown (Name server: videotraining.com: host not found) ----- Original message follows ----- CC: str-nmr@net.bio.net In-reply-to: Win'95@videotraining.com's message of 23 Sep 1995 02:53:18 GMT <43vsqu$mjc@newsie.wis.com> Subject: WINDOWS "95 VIDEO TRAINING Why are you advertising on this newgroup. Es ist verboten!! From owner-structural-nmr@net.bio.net Sat Sep 23 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!torn!nott!nrcnet0.nrc.ca!BRI.NRC.CA!Feng.Ni From: Feng.Ni@BRI.NRC.CA (Feng Ni) Newsgroups: bionet.structural-nmr Subject: Positions in Protein NMR and Molecular Design Date: 24 Sep 1995 18:26:19 GMT Organization: Institut de recherche en biotechnologie, Montréal Lines: 49 Distribution: world Message-ID: <4447sb$5hl@nrcnet0.nrc.ca> NNTP-Posting-Host: indy.bri.nrc.ca Keywords: protein NMR, protein-peptide interactions, molecular design POST-DOCTORAL AND RESEARCHER POSITIONS IN PROTEIN NMR SPECTROSCOPY AND MOLECULAR DESIGN The NMR laboratory of the Biotechnology Research Institute (BRI) anticipates the opening of one or two postdoctoral and/or researcher positions starting January, 1996 to characterize the solution structures of protein-peptide complexes. This research is part of a multi-disciplinary effort for the design of inhibitors of blood coagulation, protease regulation and signal transduction. Specific protein targets include coagulation factors (thrombin or factor Xa), cysteine protease cathepsins, epidermal growth factor receptors and protein tyrosine phosphotases (PTPases). The binding proteins for NMR work include EGF-like domains, kringle proteins, protease activation fragments and SH2 domains. Candidates for postdoctoral fellows must have a Ph.D. degree in Chemistry or Biochemistry and have demonstrated experience in NMR spectroscopy and its use in protein structure determination. Experience in protein purification is also an important asset. The researcher (RA - Research Associate) positions require postdoctoral experience in protein NMR spectroscopy or in biophysical chemistry of proteins. A successful candidate for a research associate must be able to carry out research independently and must also have good interpersonal, commu- nication and writing skills. Postdoctoral positions carry a salary of CND $23- 28K/annum and are available for upto two years. Research associates are for an initial period of two years with a salary of CND $38-45K/annum. The Biotechnology Research Institute (BRI) is a Federal research organization interfacing academia and industry. Your challenge at BRI will be to succeed in high-impact scientific research while generating interests from pharmaceu- tical industry. Qualified individuals motivated by these challenges are in- vited to forward your resume to: Feng Ni, Ph.D. Head of Biomolecular NMR Biotechnology Research Institute 6100 Royalmount Avenue Montreal, Quebec, H4P 2R2 Canada phone: (514)-496-6729 fax: (514)-496-5143 e_mail: fengni@bri.nrc.ca Applications will be accepted until the positions are filled. We thank all applicants for their interests, however, only those being considered will be contacted. From owner-structural-nmr@net.bio.net Sun Sep 24 23:00:00 1995 Path: biosci!MOLE.BIO.CAM.AC.UK!mjh2 From: mjh2@MOLE.BIO.CAM.AC.UK ("Mark J Howard ", Biochemistry) Newsgroups: bionet.structural-nmr Subject: NMR TUBES Date: 25 Sep 1995 07:30:46 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net To all who responded, Thanks very much for your information, I have passed it on to my friend, who was particularly interested in the boron background signals that can be experienced from the probe. Thanks once again. Mark Howard mjh2@mole.bio.cam.ac.uk From owner-structural-nmr@net.bio.net Sun Sep 24 23:00:00 1995 Path: biosci!daresbury!not-for-mail From: thep@risc1.lrm.fi.cnr.it (Pornthep Sompornpisut) Newsgroups: bionet.structural-nmr Subject: asking for e-mail address Date: 25 Sep 1995 12:32:45 +0100 Lines: 16 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <44640t$o7q@mserv1.dl.ac.uk> Original-To: str-nmr@dl.ac.uk Dear Colleagues, A friend of mine has been working on 3D NMR experiment. He follows an article. But it is not clear (because it was sent by FAX). He would like to contact quickly one of the following authors: Phoebe Xiurong Qi, Jeffrey L. Urbauer, E.J.Fuentes, M.F.Leopold and A.Joshua Wand. I will be appreciate if you could send us the e-mail address or fax number of one of these persons. Thank you. Pornthep From owner-structural-nmr@net.bio.net Sun Sep 24 23:00:00 1995 Path: biosci!MAILBOX.SYR.EDU!ipelczer From: ipelczer@MAILBOX.SYR.EDU (Istvan Pelczer) Newsgroups: bionet.structural-nmr Subject: Re: asking for e-mail address Date: 25 Sep 1995 05:35:09 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 42 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <44640t$o7q@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net Joshua Wand has joined SUNY at Buffalo recently; you can find the fax# of the Chemistry Department in the ACS Graduate Catalog, for example, or on the net. Regards, Istvan =========================================================================== Istvan Pelczer, Ph.D. (ipelczer@mailbox.syr.edu) Res. Assist. Professor Visiting Assist. Professor Chemistry Department, CST Bldg. SUNY ESF, Chemistry Department Syracuse University One Forestry Drive Syracuse, NY 13244-4100 Syracuse, NY 13210-2778 ph: (315) 443 1023 or x-5932 ph# (315) 470 6596 or x-6855(Dept.) fax: x-1022 or x-4070(Dept.) On 25 Sep 1995, Pornthep Sompornpisut wrote: > > Dear Colleagues, > > A friend of mine has been working on 3D NMR experiment. He follows > an article. But it is not clear (because it was sent by FAX). > He would like to contact quickly one of the following authors: > > Phoebe Xiurong Qi, Jeffrey L. Urbauer, E.J.Fuentes, > M.F.Leopold and A.Joshua Wand. > > I will be appreciate if you could send us the e-mail address or > fax number of one of these persons. > > Thank you. > > Pornthep > > From owner-structural-nmr@net.bio.net Sun Sep 24 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!swrinde!sdd.hp.com!night.primate.wisc.edu!newsspool.doit.wisc.edu!news.doit.wisc.edu!gir!elu From: elu@gir (Eldon Ulrich) Newsgroups: bionet.structural-nmr Subject: BioMagResBank Announcement Date: 25 Sep 1995 15:34:04 GMT Organization: University of Wisconsin, Madison Lines: 34 Message-ID: <446i5c$ip0@news.doit.wisc.edu> NNTP-Posting-Host: gir.nmrfam.wisc.edu X-Newsreader: TIN [version 1.2 PL2] Keywords: Pulse sequences, Software macros BioMagResBank ANNOUNCEMENT Libraries for NMR pulse sequences and software macros have been established at BioMagResBank (BMRB). The libraries can be accessed from the BMRB WWW home page, URL: http://www.bmrb.wisc.edu Each library is being built from submissions by users. If you have a favorite pulse sequence or software macro, on any topic, you are encouraged to submit it to BMRB for inclusion in the libraries. A library submission requires little more than a few details about the pulse sequence or macro as a comment in a header to an ASCII file containing the submission. The full instructions for submitting files are available from the BMRB WWW home page. A completed submission, as an ASCII file, can be sent to BMRB by anonymous ftp and placed in the appropriate directory ('incoming/pulse_sequence' or 'incoming/macro_definition'). The BMRB anonymous ftp address is: ftp.bmrb.wisc.edu We would appreciate receiving an Email message confirming that a submission has been sent (elu@nmrfam.wisc.edu). The BMRB database is undergoing rapid change. Your input and suggestions are welcomed and appreciated. Please watch the home page for further developments. From owner-structural-nmr@net.bio.net Mon Sep 25 23:00:00 1995 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.structural-nmr Subject: UNSUBSCRIBING, BIOSCI ARCHIVES, ADDRESS DATABASE & BIOSCI FAQ Date: 26 Sep 1995 02:01:00 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 309 Sender: daemon@net.bio.net Distribution: world Message-ID: <199509260900.CAA25337@net.bio.net> NNTP-Posting-Host: net.bio.net Four important items follow: How to cancel e-mail subscriptions to BIOSCI newsgroups, BIOSCI archive searching, the BIOSCI FAQ, and the BIOSCI User Address Directory form. If you have not yet listed yourself in our BIOSCI user directory, please take a few minutes to complete and return the form below. If your personal information has changed since you listed yourself, please send us a complete new updated form. We can not make manual revisions to existing entries. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net **** How to cancel a BIOSCI e-mail subscription **** If you want to cancel your e-mail subscription to this group, PLEASE DO NOT POST YOUR UNSUBSCRIBE REQUEST TO THE NEWSGROUP ADDRESS (NOR REPLY TO A MESSAGE POSTED TO THE NEWSGROUP)!!! This would send your request to all of the readers of the newsgroup, but it might still not be seen by the BIOSCI staff - thus you would annoy many people and possibly not accomplish your goal anyway. BIOSCI newsgroup instructions are available on the World Wide Web at URL http://www.bio.net/BIOSCI/docs.html. If you need personal assistance, a BIOSCI staff member can be contacted at either of the following addresses. Please contact the address designated for your location. Support Address Location --------------- -------- biosci@daresbury.ac.uk Europe, Africa, and Central Asia biosci-help@net.bio.net Americas and the Pacific Rim **** SEARCHING BIOSCI ARCHIVES **** The easiest way to search the BIOSCI archives is to use Mosaic or another World Wide Web browser and connect to the BIOSCI WWW home page at URL http://www.bio.net/. Gopher also works (to computer host net.bio.net). We maintain three indexes which are searchable: (1) an index of all BIOSCI postings; (2) an index of individual journal article references from the Table of Contents postings on the BIO-JOURNALS newsgroup; and (3) an index of BIOSCI users including regular mail and e-mail addresses, phone/FAX numbers, research interests, and newsgroup participation. E-mail users can search the BIOSCI archives by using our waismail e-mail server. For instructions send the message help to waismail@net.bio.net. Leave the Subject: line blank (anything entered on the Subject: line is ignored). **** BIOSCI FREQUENTLY ASKED QUESTIONS (FAQ) SHEET **** New users of BIOSCI/bionet may want to read the "Frequently Asked Questions" or "FAQ" sheet for BIOSCI. The FAQ provides details on how to participate in these forums and fix problems that you might encounter in using the newsgroups. The FAQ and other BIOSCI documentation is available through our WWW home page at URL http://www.bio.net/. The FAQ is also posted on the first of each month to the newsgroup BIONEWS/bionet.announce immediately following the posting of the BIOSCI information sheet. **** BIOSCI USER ADDRESS DIRECTORY **** Please take this opportunity to add your name and address information to the BIOSCI User Address Database if you have not already done so. Below is the address form that we would like each reader of the BIOSCI/bionet newsgroups to complete and return if you would like to be listed in our database. The database serves as a directory that enables biologists, who are currently using (or even just reading) the BIOSCI newsgroups, to look up e-mail addresses and other information about our users. The address database is reindexed nightly although it may sometimes take several days for new entries to appear since we need to process them prior to placing them in the indexing queue. If you are not on the Internet, please use our waismail server (send the word "help" to waismail@net.bio.net to get instructions; any text on the Subject: line of your message will be ignored, so put the help command in the body of the mail message.). Please carefully follow the instructions for completing the form below and return it to either of the following two addresses (whichever is more convenient for you). Thanks in advance for taking the time to complete and return the form. Addresses for returning forms Location Network ----------------------------- -------- ------- biovote@net.bio.net U.S.A. Internet/BITNET biovote@daresbury.ac.uk U.K. JANET MAKING SURE THAT YOUR INFORMATION IS CURRENT This notice will be mailed bimonthly to each newsgroup. You should check your database entry from time-to-time to see if your address information is still up-to-date. Using Gopher to complete the form --------------------------------- If you don't want to use a text editor, you can also use Dan Jacobson's gopher site to fill out the address database form as follows. Otherwise skip this section on gopher and proceed to the instructions for filling out the form below. > To add yourself to the database just point your > gopher client at merlot.gdb.org and select the following: > > --> 14. Searching For Biologists/ > > --> 9. E-mail Addresses of Biosci-Bionet Users/ > > --> 1. 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For example: comment: ARABIDOPSIS PLANT-BIOLOGY BIONEWS On the comment: lines use these names below ---- NOT the USENET names below MAILING LIST NAME USENET Newsgroup Name ----------------- --------------------- ACEDB-SOFT bionet.software.acedb AGEING bionet.molbio.ageing AGROFORESTRY bionet.agroforestry ARABIDOPSIS bionet.genome.arabidopsis ASCB bionet.prof-society.ascb BIOCAN bionet.prof-society.cfbs BIOFORUM bionet.general BIO-INFORMATION-THEORY bionet.info-theory BIONAUTS bionet.users.addresses BIONEWS bionet.announce BIO-JOURNALS bionet.journals.contents BIO-MATRIX bionet.molbio.bio-matrix BIOPHYSICAL-SOCIETY bionet.prof-society.biophysics BIOPHYSICS bionet.biophysics BIO-SOFTWARE bionet.software BIOTHERMOKINETICS bionet.metabolic-reg BIO-WWW bionet.software.www CARDIOVASCULAR-RESEARCH bionet.biology.cardiovascular CELEGANS bionet.celegans CELL-BIOLOGY bionet.cellbiol CHLAMYDOMONAS bionet.chlamydomonas CHROMOSOMES bionet.genome.chromosomes COMPUTATIONAL-BIOLOGY bionet.biology.computational CSM bionet.prof-society.csm CYTONET bionet.cellbiol.cytonet DROSOPHILA bionet.drosophila EMBL-DATABANK bionet.molbio.embldatabank EMF-BIO bionet.emf-bio EMPLOYMENT bionet.jobs EMPLOYMENT-WANTED bionet.jobs.wanted FASEB bionet.prof-society.faseb GDB bionet.molbio.gdb GENBANK-BB bionet.molbio.genbank GENETIC-LINKAGE bionet.molbio.gene-linkage GRASSES-SCIENCE bionet.biology.grasses HIV-MOLECULAR-BIOLOGY bionet.molbio.hiv HUMAN-GENOME-PROGRAM bionet.molbio.genome-program IMMUNOLOGY bionet.immunology INFO-GCG bionet.software.gcg JOURNAL-NOTES bionet.journals.note METHODS-AND-REAGENTS bionet.molbio.methds-reagnts MICROBIOLOGY bionet.microbiology MOLECULAR-EVOLUTION bionet.molbio.evolution MOLECULAR-MODELLING bionet.molec-model MOLLUSC-MOLECULAR-NEWS bionet.molbio.molluscs MYCOLOGY bionet.mycology NEUROSCIENCE bionet.neuroscience N2-FIXATION bionet.biology.n2-fixation PARASITOLOGY bionet.parasitology PHOTOSYNTHESIS bionet.photosynthesis PLANT-BIOLOGY bionet.plants POPULATION-BIOLOGY bionet.population-bio PROTEIN-ANALYSIS bionet.molbio.proteins PROTEIN-CRYSTALLOGRAPHY bionet.xtallography PROTISTA bionet.protista RAPD bionet.molbio.rapd SCIENCE-RESOURCES bionet.sci-resources STADEN bionet.software.staden STRUCTURAL-NMR bionet.structural-nmr TROPICAL-BIOLOGY bionet.biology.tropical URODELES bionet.organisms.urodeles VIROLOGY bionet.virology WOMEN-IN-BIOLOGY bionet.women-in-bio YEAST bionet.molbio.yeast ZBRAFISH bionet.organisms.zebrafish Listing newsgroups on the comment: line is optional, of course. Thanks again for your cooperation! --------------- please cut here and return portion below --------------- New information or Update to old record (enter N or U): date (DD-MM-YY): first name: middle initial: family name: job title: e-mail address: e-mail network: phone number: FAX number: institution: address1: address2: address3: city: state/province: country: postal code: research interest: research interest: comment: comment: comment: comment: comment: From owner-structural-nmr@net.bio.net Tue Sep 26 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!plug.news.pipex.net!pipex!tank.news.pipex.net!pipex!warwick!lyra.csx.cam.ac.uk!swhbmac16.bioc.cam.ac.uk!user From: Trumpington (LAGER) Newsgroups: bionet.structural-nmr Subject: CHAPS in NMR Date: Wed, 27 Sep 1995 15:27:34 -0200 Organization: shrubbery Lines: 5 Message-ID: NNTP-Posting-Host: swhbmac16.bioc.cam.ac.uk Does anyone know what percentage of CHAPs one can get away with in a protein sample before the linewidth become too broad due to viscosity? thanks in advance, Laurence C. Tisi lct11@uk.ac.cam.bio.mole From owner-structural-nmr@net.bio.net Sat Sep 30 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!plug.news.pipex.net!pipex!dish.news.pipex.net!pipex!tank.news.pipex.net!pipex!soap.news.pipex.net!pipex!usenet From: M J Geisow Newsgroups: bionet.structural-nmr Subject: PROTEIN STRUCTURE: EU Workprogramme and Conference Date: 1 Oct 1995 11:01:34 GMT Organization: UnipalmPIPEX server (post doesn't reflect views of UnipalmPIPEX) Lines: 26 Message-ID: <44lsee$bbp@soap.news> NNTP-Posting-Host: am079.du.pipex.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1 (Windows; I; 16bit) If you are thinking of applying for a grant in the EU Fourth Framework (Structural Biology Area) You might like to read the World Wide Web document: http://www.cryst.bbk.ac.uk/CEC/cec.html This was prepared as a concise summary for UK applicants and was checked by the European Commission. Please also note that the scientific programme for the major international Protein Engineering meeting in Montpellier, France 2-6 March 1996 is now finalised. A CEC GRANT has been applied for to support attendance by all 151 Framework 3 Structural Biology grant holders. Information and registration at: http://www.cryst.bbk.ac.uk/CEC/pope5.html Finally, note that there is now a CALENDAR of World Structural Biology Events at: http://www.cryst.bbk.ac.uk/CEC/calendar.html NOTE: PLEASE ENTER THE ADDRESSES PRESERVING THE LETTER CASE AS SHOWN. M J Geisow Sept 1995 UK LINK Protein Engineering Programme Co-ordinator From owner-structural-nmr@net.bio.net Sat Sep 30 23:00:00 1995 Path: biosci!agate!spool.mu.edu!howland.reston.ans.net!news.sprintlink.net!hermes.is.co.za!p099 From: bruce@neptune.infolink.co.za (Bruce Rae) Newsgroups: alt.sci.nmr,bionet.structural-nmr Subject: NMR Spectroscopist seeks position Date: Mon, 02 Oct 95 00:47:27 GMT Organization: No organisation supplied Lines: 30 Message-ID: <44ncqv$bmc_001@infolink.co.za> NNTP-Posting-Host: p099.infolink.co.za X-Newsreader: News Xpress Version 1.0 Beta #3 A colleague of mine in the United Kingdom is seeking a position as an NMR Spectroscopist. His name is: Dr Brian George Carter and can be contacted at home in Welwyn Garden City, Hertfordshire, United Kingdom on 1707-325497 or by e-mail: bgc0829@ggr.co.uk. He has been working as an NMR Spectroscopist since 1986 in the UK. I am prepared to post a full CV to anyone interested or you can contact him directly. ************************************************ Dr Bruce Rae +27 331 411165 (Work) Manager: Analytical Services Department Scientific Services Division Umgeni Water P O Box 9 Pietermaritzburg Natal, South Africa ************************************************ ************************************************ Dr Bruce Rae +27 331 411165 (Work) Manager: Analytical Services Department Scientific Services Division Umgeni Water P O Box 9 Pietermaritzburg Natal, South Africa ************************************************