From owner-structural-nmr@net.bio.net Tue Jul 02 23:00:00 1996 Newsgroups: bionet.structural-nmr Path: biosci!rutgers!uwm.edu!news.cse.psu.edu!news.ecn.bgu.edu!vixen.cso.uiuc.edu!howland.reston.ans.net!newsfeed.internetmci.com!in2.uu.net!fox.almaden.ibm.com!garlic.com!news.scruz.net!cruzio.com!news From: Karen Subject: (no subject) Organization: Rolf Institute Date: Tue, 2 Jul 1996 23:27:47 GMT Message-ID: X-Mailer: Mozilla 1.12APPLE (Macintosh; U; PPC) Mime-Version: 1.0 X-Url: news:bionet.structural-nmr X-Nntp-Posting-Host: wave177.cruzio.com Sender: news@cruzio.com (The News User) Lines: 0 From owner-structural-nmr@net.bio.net Thu Jul 04 23:00:00 1996 Path: biosci!biosci!not-for-mail From: ketchemr@scripps.edu (Randal R. Ketchem) Newsgroups: bionet.molec-model,bionet.molecules.peptides,bionet.molbio.proteins,sci.chem,bionet.structural-nmr Subject: Research Fellow In NMR Spectroscopy Date: 4 Jul 1996 23:16:33 -0700 Organization: University of Melbourne Lines: 46 Sender: daemon@net.bio.net Distribution: world Message-ID: <4rd3gv$kd6@riscsm.scripps.edu> NNTP-Posting-Host: net.bio.net Keywords: job, NMR Xref: biosci bionet.molec-model:1018 bionet.molecules.peptides:356 bionet.molbio.proteins:8263 sci.chem:59381 bionet.structural-nmr:1362 Date: 7/2/96 7:03 PM From: Frances Separovic Research Fellow In NMR Spectroscopy A research fellowship in NMR spectroscopy is available with the School of Chemistry, University of Melbourne, Australia. The School has a wide range of research interests in structural and solution NMR studies in organic, inorganic and biological chemistry. You, with the help of a Professional Officer, will be responsible for the daily function of the NMR Centre, technique development and training. You will be encouraged to undertake collaborative research. The NMR centre at the School of Chemistry is equipped with 4 Varian NMR spectrometers: an Inova 400, Unity Plus 400, Unity 300 and an Inova 300 for solid-state work plus 2 Sparc workstations for data processing and 20% access to a Bruker DRX 500 NMR spectrometer. Planning to gain access to higher field instruments has a high priority. You will have a relevant post graduate qualification and some experience in most solution state NMR techniques with emphasis on the structural studies of organic molecules and/or biomolecules. Experience with molecular dynamics calculations and computational methods in solution structural refinement of biological macromolecules would be particularly desirable. This appointment will be for three years (Limited Tenure). Salary: $30130 - $40889 pa (Research Fellow Grade 1). For further information, position description and the School Research Booklet contact: Associate Professor Frances Separovic (61-3) 9344 6464; fax (61-3) 9347 5180 e-mail: Frances_Separovic@muwayf.unimelb.edu.au Applications close: 27 July 1996 Reference number: Y0003776 Applications (including the names and facsimile numbers of three referees) should quote the reference number and be sent in duplicate to the Director, Human Resources, The University of Melbourne, Parkville, Victoria, 3052 Australia; fax (61-3) 9344 4694. Applications will not be acknowledged but you will be advised of the outcome. The University of Melbourne is an equal opportunity employer and has a smoke-free workplace policy. THE AUSTRALIAN UNI APPOINTS SAT 6 JULY 1996 LINE AD WITH LOGO From owner-structural-nmr@net.bio.net Sun Jul 07 23:00:00 1996 Path: biosci!rutgers!uwm.edu!vixen.cso.uiuc.edu!news.uoregon.edu!arclight.uoregon.edu!news.bc.net!unixg.ubc.ca!laue.biochem.ubc.ca!ywchen From: ywchen@laue.biochem.ubc.ca (Y. W. Chen) Newsgroups: bionet.structural-nmr Subject: membrane protein NMR review? Date: 8 Jul 1996 16:14:53 GMT Organization: University of British Columbia, Vancouver, B.C., Canada Lines: 14 Distribution: world Message-ID: <4rrc5t$f9i@nntp.ucs.ubc.ca> NNTP-Posting-Host: laue.biochem.ubc.ca Dear all, Could someone point me to a comprehensive review on the use of NMR to study membrane proteins? Thank you. -- =================================================================== Yu Wai CHEN Department of Biochemistry ................................. & Molecular Biology Faculty of Medicine tel: (604)-822 5007 2146 Health Sciences Mall fax: (604)-822 5227 University of British Columbia email: ywchen@laue.biochem.ubc.ca Vancouver BC V6T 1Z3 www: http://laue.biochem.ubc.ca:8080/wai.html CANADA =================================================================== From owner-structural-nmr@net.bio.net Sun Jul 07 23:00:00 1996 Path: biosci!CHEM.ROCHESTER.EDU!Krugh From: Krugh@CHEM.ROCHESTER.EDU (Thomas R. Krugh) Newsgroups: bionet.structural-nmr Subject: Impact of gas cylinders on NMR spectra? Date: 8 Jul 1996 05:36:06 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 41 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net The department is considering moving our 500 MHz NMR to a new location in which we would house three NMRs in the same room. The other two instruments would GE QE-300 NMRs that are used primarily by the organic chemists for routine proton and carbon-13 spectra. At present the 500 and the 300s are separate rooms. I am aware there are a number of sites where all NMRs are in one room, and I appreciate some of the advantages of the co-location of NMRs in terms of efficiency of staff support. I would appreciate comments from those who have both separate rooms and those who have common rooms of the advantages and disadvantages of a shared environment, especially in terms of any impact of using the 500 for the types of long-term experiments needed for structural studies of nucleic acids and proteins. Will the steady stream of graduate students using the 300's create any problems? How much isolated space around the 500 solenoid is necessary to minimize impact on structural studies? Is noise (fan noise and people noise) a factor? How is this handled? We also have to consider the potential impact on 2D spectra from movement of gas cylinders in the laboratory one floor above. The floor of the laboratory above is approximately 3 meters from the center of the solenoid. I recall that shortly after we set up our 500 (nine years ago) we found that movement of a gas cylinder in the lab above the present location resulted in a measurable effect on NMR spectra. Fortunately, this lab rarely moves cylinders in the area above the solenoid. Do others have experience with the impact of the movement of gas cylinders (both high pressure gas cylinders and liquid nitrogen cylinders)? I plan to measure the effect and request your comments on the type of experiments that may be used to demonstrate the magnitude of the effect. Thanks, Tom Krugh _________________________________________________________________________ Thomas R. Krugh Krugh@chem.rochester.edu Department of Chemistry Phone: (716) 275-4224 University of Rochester FAX: (716) 473-6889 Rochester, NY 14627 _________________________________________________________________________ From owner-structural-nmr@net.bio.net Sun Jul 07 23:00:00 1996 Path: biosci!UCHSC.EDU!Michael.Overduin From: Michael.Overduin@UCHSC.EDU (Michael Overduin) Newsgroups: bionet.structural-nmr Subject: NMR Manager position Date: 8 Jul 1996 11:10:49 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 38 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Announcing a Job Opportunity: NMR MANAGER at the University of Colorado Health Sciences Center The University of Colorado Cancer Center and the Department of Pharmacology invite applications for a research track position at the assistant professor level. The successful individual will manage a newly established NMR facility which is part of an institutional Macromolecular Structure Program. Responsibilities include maintaining 600 and 500 MHz Varian INOVA spectrometers (4-channel, with triple resonance PFG probes), instructing new users, and the development of a research program related to biomolecular function. A 2500 square foot space is being renovated to house four spectrometers, computer, wet-lab and office space. The NMR Manager will play a major role in deciding user policies and in outfitting/upgrading the facility (significant funds are available from a recently awarded Howard Hughes Medical Institute grant). The core of the NMR community at the University includes myself and Brad Bendiak. There are many opportunities for collaborative research with investigators who already include NMR as a component of their research. A X-ray crystallography facility is being built nearby, and an established EM facility is being moved across the hall from the NMR facility. A mass spectroscopy facility is available, and the Cancer Center operates 16 Core facilities including NMR, fermentation, DNA and protein sequencing. We intend to fill this position by late 1996 or early 1997. Applicants must have a Ph.D. degree, background in NMR theory, experience in spectrometer function, and potential to develop extramural funding. Please submit curriculum vitae, names of three references, and a statement of research interests to: Chair, NMR Search Committee Campus Box C236 University of Colorado Health Sciences Center 4200 East Ninth Avenue, Denver, CO 80262 From owner-structural-nmr@net.bio.net Mon Jul 08 23:00:00 1996 Path: biosci!ns1.faseb.org!lamarck.sura.net!ra.nrl.navy.mil!news.math.psu.edu!news.cse.psu.edu!uwm.edu!spool.mu.edu!usenet.eel.ufl.edu!tank.news.pipex.net!pipex!oleane!jussieu.fr!Newsmaster From: Michel SEIGNEURET Newsgroups: bionet.structural-nmr Subject: J coupling measurements Date: 9 Jul 1996 06:57:51 GMT Organization: Universites Paris VI/Paris VII - France Lines: 27 Message-ID: <4rsvtf$foo@vishnu.jussieu.fr> NNTP-Posting-Host: mood.biophy.jussieu.fr Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (X11; I; IRIX 5.3 IP20) X-URL: news:bionet.structural-nmr Dear Collegues, We are studying membrane peptides in detergent micelles. Due to relatively large linewidths, J coupling values cannot directly be measured on COSY spectra and we routinely use a FELIX routine which involves gaussian fitting the F2 projections of both the upper and lower antiphase doublets. Typically, for an helical peptide, the measured NH-alphaH splitting values are around 6-7 Hz and the FELIX routine yields correct J coupling values around 5 Hz. We have now a peptide which forms a larger detergent complex and for which this procedure does not work. The measured NH-alphaH splitting values are 9-11 Hz and the FELIX routine gives values of about 7-9 Hz which are clearly incorrect since the peptide is helical (based on NH-NH NOESY's). Are there more accurate methods that allow one to recover J coupling values for such extreme cases ? Thank you in advance. Michel M. Seigneuret Lab. de Biophysique Cellulaire et RMN Universite Paris 7 France e-mail: michel@biophy.jussieu.fr From owner-structural-nmr@net.bio.net Mon Jul 08 23:00:00 1996 Path: biosci!st-andrews.ac.uk!ss4 From: ss4@st-andrews.ac.uk (Sina Sareth) Newsgroups: bionet.structural-nmr Subject: NMR mailing list Date: 9 Jul 1996 06:51:26 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I would like to join the NMR mailing list Thank you Sina From owner-structural-nmr@net.bio.net Wed Jul 10 23:00:00 1996 Path: biosci!rutgers!sgigate.sgi.com!news-res.gsl.net!news.gsl.net!news.uoregon.edu!news.rediris.es!power.ci.uv.es!usenet From: aranae@uv.es (E. Arana) Newsgroups: bionet.structural-nmr Subject: Help:low vs high MR field Date: Thu, 11 Jul 1996 05:01:37 GMT Organization: Universitat de Valencia, Spain Lines: 32 Message-ID: <4s21su$fb0@power.ci.uv.es> NNTP-Posting-Host: aport1.ci.uv.es X-Newsreader: Forte Free Agent 1.0.82 Hello Everyone reading this: As the Chief of the MR Section on the Department of Radiology, I am greatly interesting in MRI equipment, and the differences between high versus low field. It should be a pleasure if you could send me your opinions regarding some topics: 1. Is there any randomized clinical trial about the diagnostic accuracy of these different field strengths? 2. Does any one know of any real benefit of using high field versus low field MR equipments? 3. Does the decision of use high versus low field have any impact on patient management? 4. Is there any cost-effectiveness or cost-efficacy study on this topic? 5. Is there any limitation on the use on contrast media regarding MR field strength? 6. Is there any clinically relevant limitation of low-medium field magnets? 7. Are there any important security features regarding high field and gradients strength? Thank you very much in advance bonmati@san.gva.es Hospital Dr. Peset Valencia. Spain. From owner-structural-nmr@net.bio.net Wed Jul 10 23:00:00 1996 Path: biosci!PHOENIX.PRINCETON.EDU!ipelczer From: ipelczer@PHOENIX.PRINCETON.EDU (Istvan Pelczer) Newsgroups: bionet.structural-nmr Subject: buffer Date: 11 Jul 1996 08:30:24 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 21 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear Netters, I have various suggestions how to make a ubiquitin sample for test measurements. One of them is about to use potassium phosphate buffer (25-50 mM). Would any of you have experience with such a setup? Any other comments are welcome, too. Thanks, Istvan wwww,wwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwww Istvan Pelczer, Ph.D. Email: ipelczer@princeton.edu Senior NMR Spectroscopist Princeton University Department of Chemistry ph# (609) 258 2342 Frick Laboratory fax# (609) 258 6746 Washington Road Princeton, NJ 08544 From owner-structural-nmr@net.bio.net Thu Jul 11 23:00:00 1996 Path: biosci!rutgers!uwm.edu!cs.utexas.edu!howland.reston.ans.net!newsfeed.internetmci.com!news.ycc.yale.edu!yale!news.wesleyan.edu!voltaire.chem.wesleyan.edu!user From: dsprous@rose.chem.wesleyan.edu (Dennis Sprous) Newsgroups: bionet.structural-nmr Subject: FIRST ELECTRONIC MOLECULAR GRAPHICS AND MODELLING SOCIETY CONFERENCE Date: Fri, 12 Jul 1996 09:02:37 -0500 Organization: Wesleyan University Middletown CT 06459 Lines: 316 Message-ID: NNTP-Posting-Host: voltaire.chem.wesleyan.edu X-Newsreader: Value-Added NewsWatcher 2.12d0+ FIRST ELECTRONIC MOLECULAR GRAPHICS AND MODELLING SOCIETY CONFERENCE ******************************************************************** The First Electronic Molecular Graphics and Modelling Society Conference (MGMS EC-1) will be held on the Internet (the Net) and World Wide Web (the Web) from Oct 7-18, 1996. The conference is sponsored by Elsevier and the Molecular Graphics and Modelling Society and will be co-organized by Graham Richards, Art Olson, Rod Hubbard and Barry Hardy. MGMS EC-1 will be a fully international event open to all members of the scientific community and will cover a broad range of disciplines related to molecular modelling, graphics and simulation methods and applications. Conference subject areas are: Protein Structure; Membranes and Membrane Proteins; Bioinformatics; Computational Nanotechnology; Protein Folding; Modelling of In Vivo Activity; Knowledge-based Library Design; Surface Science; Host-guest interactions; Carbohydrates; Enzyme Mechanisms; Conformational Analysis; Nucleic Acids; Quantum Chemistry; Car-Parrinello Methods and Applications; Structure-based Design; Visualization; and Perspectives. Each subject area has a section convener who will screen abstracts sent by authors for suitability. Authors can opt to submit a non-permanent presentation like a normal conference poster or a presentation which will be refereed and considered for publication in the Journal of Molecular Graphics. Referees will be appointed by the section conveners. Presentations must be prepared in Hypertext Markup Language (HTML), graphics (GIF,JPEG) and other web-compatible formats (VRML,Java,PDF) so that participants can view the papers via the World Wide Web. Aid and consultation will be provided to participants in the weeks prior to the event to help them with their presentation (Email:mgmsorg@bellatrix.pcl.ox.ac.uk). Further details will be given in the authors' guide accessible via the URL: http://bellatrix.pcl.ox.ac.uk/mgms/ During the conference discussions will take place via the Internet in real-time using a virtual conference centre based on a MOO (multiple-user domain, object oriented) and via Internet-accessible electronic mailing lists. Trial sessions for those not familiar with MOOs will be held before the conference. Before the conference, a timetable for MOO discussion sessions of each section will be posted. Since these realtime discussions are an integral part of the conference, authors will be expected to attend one for their subject; the right is reserved not to referee submissions by authors who do not attend one of these sessions. The Conference will feature a Virtual Trade Center where commercial vendors, software and hardware developers, consultants, and contractors will be able to display their goods and services and provide software demonstrations in return for exhibition fees to support conference activities. Any potential exhibitors should contact Barry Hardy at barry@bellatrix.pcl.ox.ac.uk ******************* DEADLINES AND DATES 1) DO NOW - The MGMS mailing list Conference-related news and announcements will be posted regularly to the MGMS mailing list (http://bellatrix.pcl.ox.ac.uk/hypermail/mgms/). If you wish to subscribe to the MGMS list send the following one line message to majordomo@bellatrix.pcl.ox.ac.uk: subscribe mgms@bellatrix.pcl.ox.ac.uk your_email@address your_name 2) DO NOW - Registration The deadline for registration is Sept 15th 1996. Early registration is strongly encouraged to aid the efficient operation of the conference including the establishment of timely access to the conference. If you intend to participate in MGMS EC-1 please use the registration form accessible via http://bellatrix.pcl.ox.ac.uk/mgms/ which is available for electronic registration. The registration will be used to construct a registrant database for the conference which will generate the conference mailing list and handle assignment of userids and passwords. In addition it is necessary to pay for registration via ordinary means: The conference fee will be 35 pounds sterling (50 US dollars) with a special rate for students of 20 pounds sterling (30 US dollars). A copy of a suitable student identification or a letter from the supervisor will be required to be charged at the lower rate. Registration payments can be accepted by credit card, direct bank transfer, cheque or bank draft. Regardless of method of payment we ask you to supply accompanying correspondence with your complete name, address, fax and phone number, and email address which should be mailed directly to: Dr. Barry Hardy, Physical and Theoretical Chemistry Laboratory, University of Oxford, South Parks Road, Oxford, OX1 3QZ, UK Payment Instructions: a) Credit Card We can currently accept Visa, Mastercard, Switch or JCB. We cannot yet accept American Express as our application with them has still to be processed. Please send your credit card type, number and expiration date via regular mail to: Dr. Barry Hardy, Physical and Theoretical Chemistry Laboratory, University of Oxford, South Parks Road, Oxford, OX1 3QZ, UK b) Bank Transfer Your payment can be made in sterling by direct bank transfer into the folowing account: Account Number: 20-6518 50772712 Account Name: Greenlea Communications Bank: Barclays Bank, Oxford City Centre Branch, P.O. Box 333, Oxford, OX1 3HS, UK --Please ensure your full name is listed with the transfer details. --You are responsible for any bank charges associated with the transfer from your bank. --Please notify us directly of your transfer payment. 3) Cheque/Bank Draft Cheques or bank orders (in pounds or dollars) should be made out to Greenlea Communications and mailed to: Dr. Barry Hardy, Physical and Theoretical Chemistry Laboratory, University of Oxford, South Parks Road, Oxford, OX1 3QZ, UK Acknowledgement of Registration and Payment: When you first register at the conference site you should receive an acknowledgement email with a password and userid. After receipt of your registration fee you will receive a further acknowledgement and your userid and password will be validated to allow access to the conference site (when it opens). (Note: Registration payments received during the period July 23- August 14 will not be immediately processed and acknowledged. They will be processed as soon after August 14 as possible.) Academic registrants from economically-disadvantaged countries can write to Barry Hardy requesting an exemption to the registration fee. Exemptions will be made on a discretionary basis taking into account the reasons given for the request and will be dependent on suitable funds being available. We will consider economically disadvantaged countries to include those of Eastern Europe, Africa, Central and South America, Indian sub-continent, etc. 3) DEADLINE for receipt of ABSTRACT. The deadline for receipt of presentation abstracts is August 1. Email your abstract directly to the appropriate section convener listed below. Fuller details of the scope of each section will be given in the authors' guide accessible via http://bellatrix.pcl.ox.ac.uk/mgms/ Your abstract should be no longer than 300 words. And remember to state which category of presentation (non-permanent poster or refereed paper) you wish. If you are unsure as to which section your abstract is suitable for, please email a possible section convener or Barry Hardy (barry@bellatrix.pcl.ox.ac.uk). Section conveners do have the discretion to reject abstracts, ask for revisions to an abstract or to send the abstract to the convener of a more suitable section. 4) DEADLINE for receipt of PRESENTATION The deadline for receipt of papers and posters is Sept 15th. You must deposit your text and graphics files at the conference ftp site for presentation at the conference. Ftp instructions will be provided at the conference site. Hard copies of final refereed papers following the format of the Journal of Molecular Graphics should be sent by Dec 1 to: Graham Richards, Journal of Molecular Graphics, Physical & Theoretical Chemistry Lab, Sth Parks Rd, University of Oxford, Oxford, OX1 3QZ, UK. Journal guidelines are posted at http://bellatrix.pcl.ox.ac.uk/mgms/ Although there is no limit to the graphics authors choose to display in their conference presentation, the editor reserves the right to limit the number of free colour figures in each printed journal article. 5) Refereeing Period The refereeing period will commence upon completion of the conference. If you have a presentation at MGMS EC-1 you may be requested to contribute a refereeing evaluation on another conference presentation. Referee reports will be due November 15. ******************* SECTIONS AND SECTION CONVENERS (Please email section conveners with questions related to your particular section and presentation. Details on the sections are accessible via http://bellatrix.pcl.ox.ac.uk/mgms/) Any general emails (such as registration queries, maillist queries, HTML queries, password queries, timetable queries, general technical advice on browsers and graphics, MOO queries, etc.) should be sent to: mgmsorg@bellatrix.pcl.ox.ac.uk 1. Protein Structure Rod Hubbard University of York, UK Email:rod@yorvic.york.ac.uk 2. Nucleic Acids David Beveridge and Dennis Sprous Wesleyan University, USA Email:bever@rose.chem.wesleyan.edu,dsprous@rose.chem.wesleyan.edu 3. Membranes and Membrane Proteins Alan Robinson University of Oxford, UK Email:alan@bellatrix.pcl.ox.ac.uk and Terry Stouch Bristol-Myers Squibb, USA Email:stouch@dino.bms.com 4. Bioinformatics Steve Gardner Oxford Molecular, UK Email:sgardner@oxmol.co.uk 5. Computational Nanotechnology Al Globus NASA-Ames, USA Email:globus@nas.nasa.gov 6. Protein Folding Jeffrey Skolnick Scripps Institute, USA Email:skolnick@scripps.edu 7. Modelling of In Vivo Activity Edward Hodgkin Wyeth-Ayerst Research, USA Email:hodgkie@war.wyeth.com 8. Knowledge-based Library Design Mike Hann GlaxoWellcome, UK Email:mmh1203@ggr.co.uk 9. Surface Science Donald Brenner North Carolina State, USA Email:dwb@ripley.mte.ncsu.edu 10. Host-Guest interactions Jon Essex Southampton University, UK Email:J.W.Essex@soton.ac.uk 11. Carbohydrates and Protein-Carbohydrate Interactions Anne Imberty CNRS, France Email:imberty@nantes.inra.fr 12. Enzyme Mechanisms Guy Grant University College Dublin, Ireland Email:ggrant@macollamh.ucd.ie 13. Stochastic Methods for Conformational Sampling Robert Topper The Cooper Union, USA Email:topper@cooper.edu 14. Quantum Chemistry Tim Clark University of Erlangen, Germany Email:clark@organik.uni-erlangen.de 15. Structure-based Design David Winkler CSIRO, Australia Email:D.Winkler@chem.csiro.au 16. Car-Parrinello Methods and Applications Michele Parrinello Max-Planck Institute, Germany Email:prr@prr.mpi-stuttgart.mpg.de 17. Visualization Art Olson Scripps Institute, USA Email:olson@scripps.edu 18. Perspectives in Molecular Modelling Graham Richards University of Oxford, UK Email:gr@vax.ox.ac.uk From owner-structural-nmr@net.bio.net Thu Jul 11 23:00:00 1996 Path: biosci!agate!spool.mu.edu!uwm.edu!lll-winken.llnl.gov!nntp.coast.net!news00.sunet.se!sunic!news99.sunet.se!news.uni-c.dk!news From: Mogens Kjaer Newsgroups: bionet.structural-nmr Subject: Re: J coupling measurements Date: Wed, 10 Jul 1996 10:07:20 +0200 Organization: Carlsberg Laboratory Lines: 33 Message-ID: <31E364B8.ABD@unidhp.uni-c.dk> References: <4rsvtf$foo@vishnu.jussieu.fr> NNTP-Posting-Host: lgb275.ppp.uni-c.dk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0b5aGold (X11; I; IRIX 5.3 IP22) To: Michel SEIGNEURET Michel SEIGNEURET wrote: > > Are there more accurate methods that allow one to recover J coupling values for > such extreme cases ? > > Thank you in advance. > > Michel > > M. Seigneuret > Lab. de Biophysique Cellulaire et RMN > Universite Paris 7 > France > e-mail: michel@biophy.jussieu.fr Hi Michel, You could try the algorithm used in Pronto. You'll need both a COSY and a NOESY spectrum to do this, see the manual http://unidhp.uni-c.dk/~carlmk/ch12.html You can download a copy by following the URL in the signature. Mogens BTW your returnaddress is wrong. -- Mogens Kjaer, Carlsberg Laboratory, Dept. of Chemistry Gamle Carlsberg Vej 10, DK-2500 Valby, Denmark Phone: +45 33 27 53 25, Fax: +45 33 27 47 08 Email: carlmk@unidhp.uni-c.dk Homepage: http://unidhp.uni-c.dk/~carlmk From owner-structural-nmr@net.bio.net Thu Jul 11 23:00:00 1996 Path: biosci!rutgers!uwm.edu!lll-winken.llnl.gov!nntp.coast.net!news00.sunet.se!sunic!news99.sunet.se!news.uni-c.dk!news From: Mogens Kjaer Newsgroups: bionet.structural-nmr Subject: Re: J coupling measurements Date: Wed, 10 Jul 1996 10:06:14 +0200 Organization: Carlsberg Laboratory Lines: 30 Message-ID: <31E36476.FF6@unidhp.uni-c.dk> References: <4rsvtf$foo@vishnu.jussieu.fr> NNTP-Posting-Host: lgb275.ppp.uni-c.dk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0b5aGold (X11; I; IRIX 5.3 IP22) To: Michel SEIGNEURET Michel SEIGNEURET wrote: > > Are there more accurate methods that allow one to recover J coupling values for > such extreme cases ? > > Thank you in advance. > > Michel > > M. Seigneuret > Lab. de Biophysique Cellulaire et RMN > Universite Paris 7 > France > e-mail: michel@biophy.jussieu.fr Hi Michel, You could try the algorithm used in Pronto. You'll need both a COSY and a NOESY spectrum to do this, see the manual http://unidhp.uni-c.dk/~carlmk/ch12.html You can download a copy by following the URL in the signature. Mogens -- Mogens Kjaer, Carlsberg Laboratory, Dept. of Chemistry Gamle Carlsberg Vej 10, DK-2500 Valby, Denmark Phone: +45 33 27 53 25, Fax: +45 33 27 47 08 Email: carlmk@unidhp.uni-c.dk Homepage: http://unidhp.uni-c.dk/~carlmk From owner-structural-nmr@net.bio.net Sat Jul 13 23:00:00 1996 Path: biosci!ihnp4.ucsd.edu!swrinde!news-res.gsl.net!news.gsl.net!hunter.premier.net!insync!uunet!inXS.uu.net!news.shout.net!news From: Gary Turner Newsgroups: bionet.structural-nmr Subject: Used MR Equipment Newsletter/site Date: Sun, 14 Jul 1996 17:09:26 -0700 Organization: Spectral Data Services, Inc. Lines: 34 Message-ID: <31E98C36.358B@sdsnmr.com> NNTP-Posting-Host: sgtdial16.shout.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Win16; I) The Larmor Letter is a free on-line newsletter for announcements of used NMR, MRI, and EPR equipment for sale, wanted, or for trade. This newsletter is distributed freely to all concerned, and there is no charge to list your announcement. There are no restrictions as to whom may submit announcements. Types of equipment can be spectrometers, probes, consoles, amplifiers, "spare parts", or any other equipment used in MR. To submit an announcement for MR equipment available or wanted, send a brief description of the equipment to sdsnmr@sdsnmr.com. Include the name of a contact person, and their email address and phone/fax number. Please note that The Larmor Letter will neither buy, sell, or trade equipment, or make recommendations. The Larmor Letter will not assume any liability for non-performance of any equipment listed herein. The Larmor Letter will not act as an agent in any transaction. Anyone wishing to receive this free newsletter should send a request to sdsnmr@sdsnmr.com. This newsletter can also be viewed at http://www.sdsnmr.com The website also has links to other pre-owned equipment sites. ==================================================================== Gary Turner Spectral Data Services, Inc. Contract NMR Data Acquisition phone : (217)352-7084 fax : (217)352-9748 email : sdsnmr@sdsnmr.com http://www.sdsnmr.com From owner-structural-nmr@net.bio.net Sun Jul 14 23:00:00 1996 Path: biosci!rutgers!uwm.edu!news-res.gsl.net!news.gsl.net!hunter.premier.net!netnews.worldnet.att.net!uunet!inXS.uu.net!news.tufts.edu!usenet From: Christoph Steinbeck Newsgroups: bionet.structural-nmr Subject: Re: Here is my story Date: Mon, 15 Jul 1996 18:30:48 -0400 Organization: Dep. of Chemistry, Tufts University, Medford Lines: 25 Message-ID: <31EAC698.41C6@microvirus.chem.tufts.edu> References: <4se1f4$iqo@netnews.upenn.edu> NNTP-Posting-Host: microvirus.chem.tufts.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0b5aGold (X11; I; IRIX 5.3 IP22) Bill Hamilton wrote: > > Zamboni here. had good weekend. got new pet. new pet is frog. Zamboni like frogs. frogs are green. green > is second favorite color. purple favorite color. frogs are slimy. slimy is good. poop is slimy. like poop. name > frog stanley. introduce stanley to horatio. horatio is my cock. hello horatio. hello stanley. they are my friends. > they talk to me. they talk telepathically. introduce stanley to henrietta. henrietta my hand. henrietta and > horatio love each other. they always horny. they like stanley. Stuff deleted... ---------------------------------------------------------------------------- > mom. that crazy chick. smell like fish. taste like chicken. > my respect to Kibo. may he watch over Zamboni and keep him safe. > ---------------------------------------------------------------------------- Did I miss something? -- Dr. Christoph Steinbeck mailto:stein@microvirus.chem.tufts.edu http://www.tufts.edu/~csteinbe/ Dep. of Chemistry, Tufts University, 62 Talbot Ave., Medford, Massachusetts 02155 Phone: (617) 627-3881 FAX: (617) 627-3443 *** Get my PGP public key via http://www.tufts.edu/~csteinbe/#PGP *** From owner-structural-nmr@net.bio.net Sun Jul 14 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: jody McGill Newsgroups: bionet.structural-nmr Subject: Principles of Protein Structure Using the Internet (fwd) Date: 15 Jul 1996 14:10:14 +0100 Lines: 23 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4sdfvm$1f7@mserv1.dl.ac.uk> X-Sender: jody@iona.cryst.bbk.ac.uk Original-To: To:;@mail.cryst.bbk.ac.uk; Dear All The Crystallography Department of Birkbeck College is running a part-time, London University course on the PRINCIPLES OF PROTEIN STRUCTURE on the Internet. 1. Introduction to Internet Resources 2. Protein Structure 3. Dissertation: structure, function and dynamics The course covers one academic year of three terms, from September 30, 1996 to 4 July 1997. Costs are: 250 pounds sterling for EU students and 550 pounds sterling for other students. For details of course contents, administration and registration URL http://www.cryst.bbk.ac.uk/PPS2/index.html Contact: Jody McGill, Crystallography Department, Birkbeck College London, WC1E 7HX. UK Tel. +44 (0)171 631 6800 Fax. +44 (0)171 631 6803 e.mail: j.mcgill@mail.cryst.bbk.ac.uk From owner-structural-nmr@net.bio.net Sun Jul 14 23:00:00 1996 Path: biosci!NARCISSUS.CHEM.SUNYSB.EDU!smita From: smita@NARCISSUS.CHEM.SUNYSB.EDU (smita mohanty) Newsgroups: bionet.structural-nmr Subject: Shigami NMR tubes Date: 15 Jul 1996 12:24:02 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: <199607151938.PAA01363@narcissus.chem.sunysb.edu> NNTP-Posting-Host: net.bio.net I would appreciate it very much if anyone who has bought Shigami nmr tubes for high field Varian instruments, can let me know the address and phone number of the supplier. Thank you very much. Smita Mohanty, Ph.D Chemistry Department State University of New York at Stony Brook Stony Brook, Ny-11794-3400 E.mail: smita@narcissus.chem.suny From owner-structural-nmr@net.bio.net Mon Jul 15 23:00:00 1996 Path: biosci!NMRSGI2.NCIFCRF.GOV!rabyrd From: rabyrd@NMRSGI2.NCIFCRF.GOV (R. Andrew Byrd) Newsgroups: bionet.structural-nmr Subject: XVIIth ICMRBS Date: 16 Jul 1996 13:10:55 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 48 Sender: daemon@net.bio.net Distribution: world Message-ID: <199607162004.QAA03233@nmrsgi2.ncifcrf.gov> NNTP-Posting-Host: net.bio.net PROGRAM UPDATE!! XVIIth International Confernce on Magnetic Resonance in Biological Systems August 18-23, 1996 Keystone, Colorado We are pleased to announce that the final program for the conference, including authors and titles, has been posted on the web page. http://nmrsgi1.ncifcrf.gov/icmrbsxvii/ You will find both the lecture program and a listing of the poster sessions. Each segment of the program is available in several formats to assist participants in searching and locating items of interest. The listings on the web will serve as the only pre-conference program which is available. Please use this listing as your means of planning your attendance. I would also like to indicate that many aspects of the web page have been updated, including information concerning conference sponsors, exhibitors and hospitality suites. We will continue to add new information as appropriate. Those who have already registered may wish to check the page to be sure that they have all the appropriate information. Although we can no longer accept abstracts, we are still accepting registrations. Please consult the web page for information. On behalf of the Organizing Committee, I look forward to welcoming all the particpants to an exciting meeting in Keystone. Andy Byrd XVIIth ICMRBS ------------------ Dr. R. Andrew Byrd Macromolecular NMR/MSL/ABL TEL: 301-846-1407 FAX: 301-846-6195 From owner-structural-nmr@net.bio.net Mon Jul 15 23:00:00 1996 Path: biosci!HELIX.NIH.GOV!huang From: huang@HELIX.NIH.GOV (Xialing Huang) Newsgroups: bionet.structural-nmr Subject: re: Problem with solvent TFE Date: 16 Jul 1996 05:44:35 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 21 Sender: daemon@net.bio.net Distribution: world Message-ID: <199607161244.IAA24127@helix.nih.gov> NNTP-Posting-Host: net.bio.net Dietmar Kaiser wrote: > After using DMSO, methanol and water as NMR solvents I just started > to do NMR of a peptide in TFE-d2. > My problem is that it is almost impossible to shim the sample. > The lines are very broad, up to tens of Hertz for the OH signal. > I know that TFE is said to produce broader lines but this seems to > much to me. > I can change the shim settings by several hundred units > ( Bruker BSMS) and almost nothing happens to the lock signal. the lineshape > test is ok and gives a linewidth according to the specification. > Has anybody out there made similar experiences with TFE as a solvent? Hi, Dear D. Kaiser I have similar experiences with TFE as a solvent. Please try to use mixture of TFE and H20 (v/v 90:10 or 90:5). I hope it helps. Good luck. Xiaolin huang From owner-structural-nmr@net.bio.net Mon Jul 15 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: dietmar@akjung5.orgchemie.chemie.uni-tuebingen.de (Dietmar Kaiser) Newsgroups: bionet.structural-nmr Subject: Problem with solvent TFE Date: 16 Jul 1996 05:16:17 +0100 Lines: 19 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4sf52h$78r@mserv1.dl.ac.uk> Original-To: str-nmr@dl.ac.uk Hi all, After using DMSO, methanol and water as NMR solvents I just started to do NMR of a peptide in TFE-d2. My problem is that it is almost impossible to shim the sample. The lines are very broad, up to tens of Hertz for the OH signal. I know that TFE is said to produce broader lines but this seems to much to me. I can change the shim settings by several hundred units ( Bruker BSMS) and almost nothing happens to the lock signal. the lineshape test is ok and gives a linewidth according to the specification. Has anybody out there made similar experiences with TFE as a solvent? desperately, Dietmar Kaiser From owner-structural-nmr@net.bio.net Mon Jul 15 23:00:00 1996 Path: biosci!RIKENNMR.RIKEN.GO.JP!uzawa From: uzawa@RIKENNMR.RIKEN.GO.JP Newsgroups: bionet.structural-nmr Subject: Re:Shigemi NMR tubes Date: 15 Jul 1996 18:43:07 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 22 Sender: daemon@net.bio.net Distribution: world Message-ID: <9607160143.AA00555@rikennmr.riken.go.jp> NNTP-Posting-Host: net.bio.net Dear Dr.Smita Mohanty, >I would appreciate it very much if anyone who has bought >Shigami nmr tubes for high field Varian instruments, can >let me know the address and phone number of the supplier. Shigemi's address and phone number is here, TEL:(412)444-3011, FAX:(412)444-3020 SHIGEMI,INC. SUITE 21, 4790 ROUTE 8,ALLISON PARK, PA 15101 U S A ------------------------------------------------------------ Jun UZAWA, Ph.D The Institute of Physical and Chmical Research(RIKEN) Hirosaw 2-1, Wako, Saitama 351-01 JAPAN TEL 81-48-467-9361 FAX 81-48-462-4627 e-mail uzawa@rikennmr.riken.go.jp ----------------------------------------------------------- From owner-structural-nmr@net.bio.net Wed Jul 17 23:00:00 1996 Path: biosci!bcm.tmc.edu!watson!amcgough From: amcgough@bcm.tmc.edu (Amy McGough) Newsgroups: bionet.structural-nmr Subject: Symposium on Computational Biology Date: 18 Jul 1996 22:33:56 GMT Organization: Baylor College of Medicine, Houston, Tx Lines: 71 Message-ID: <4sme4k$mlk@gazette.bcm.tmc.edu> NNTP-Posting-Host: bcm.tmc.edu NNTP-Posting-User: amcgough X-Newsreader: TIN [version 1.2 PL2] The abstract deadline for the Keck Center/ASBMB Fall Symposium has been moved to July 25. For those who are very interested in submitting but who may require a few additional days, please contact Marc Archambault (march@bioc.rice.edu) to inquire about an extension. CALL FOR ABSTRACTS *** SYMPOSIUM ANNOUNCEMENT "Computational Biology: Methods in Biomolecular Imaging" an American Society for Biochemistry and Molecular Biology Fall Symposium, Co-Sponsored by the W. M. Keck Center for Computational Biology, Houston, TX DATE & LOCATION: 25-28 October 1996, Whistler, British Columbia, Canada WEB SITES: http://www.faseb.org/meetings/asbmb/asbmbhp.htm AND http://ncmi.bioch.bcm.tmc.edu/~amy/ASBMB.html Organized by Kathleen S. Matthews, Rice University; Wah Chiu, Baylor College of Medicine; B. Montgomery Pettitt, University of Houston; and George N. Phillips, Jr., Rice University Keynote Address, Michael Rossmann, Purdue University Experimental Structural Methods, Keith Moffat, University of Chicago Computational Structural Methods, George Phillips, Jr., Rice University Experimental Imaging Methods, Wah Chiu, Baylor College of Medicine Computational Imaging Methods, Gerard Bricogne, Medical Research Council, Cambridge, UK Graphics & Visualization, Bridget Carragher, University of Illinois, Urbana Other Invited Speakers include: Michael Pique, Scripps Research Institute Axel T. Brunger, Yale University Kenneth H. Downing, UC, Berkeley Helen Hansma, UC, Santa Barbara Wayne A. Hendrickson, Columbia University Richard Tapia, Rice University L. Ridgway Scott, University of Houston Marin van Heel, Imperial College, London Edgar Weckert, Universitaet Karlsruhe Kurt Wuthrich, ETH Zurich Abstract Deadline: July 25, 1996 (direct inquirys about extensions to march@bioc.rice.edu) Room Reservations: September 25, 1996 Advance Registration: October 11, 1996 For more information contact: ASBMB 9650 Rockville Pike Bethesda, MD 20814-3998 FAX: (301) 530-7014 Telephone: (301) 530-7010 Email: gswindle@osmc.faseb.org or: Marc L. Archambault, Executive Director W. M. Keck Center for Computational Biology Rice University MS 141 6100 S. Main Street Houston, TX 77005 FAX: +1 (713) 527-4659 or +1 (713) 285-5454 Telephone: (713) 527-4752 E-mail: march@rice.edu From owner-structural-nmr@net.bio.net Thu Jul 18 23:00:00 1996 Path: biosci!rutgers!uwm.edu!lll-winken.llnl.gov!nntp.coast.net!fu-berlin.de!main.Germany.EU.net!Germany.EU.net!wizard.pn.com!brighton.openmarket.com!decwrl!news.hooked.net!usenet From: Eddy Jakobovits Newsgroups: bionet.structural-nmr Subject: Re: XVIIth ICMRBS Date: Fri, 19 Jul 1996 11:49:40 -0700 Organization: SciWeb Inc. Lines: 71 Message-ID: <31EFD8C4.35AB@sciweb.com> References: <199607162004.QAA03233@nmrsgi2.ncifcrf.gov> NNTP-Posting-Host: sole-21.ppp.hooked.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0b5aGold (Win95; I) To: "R. Andrew Byrd" R. Andrew Byrd wrote: > > PROGRAM UPDATE!! > > XVIIth International Confernce on Magnetic > Resonance in Biological Systems > > August 18-23, 1996 > Keystone, Colorado > > We are pleased to announce that the final program for the > conference, including authors and titles, has been posted > on the web page. > > http://nmrsgi1.ncifcrf.gov/icmrbsxvii/ > > You will find both the lecture program and a listing of > the poster sessions. Each segment of the program is > available in several formats to assist participants in > searching and locating items of interest. > > The listings on the web will serve as the only pre-conference > program which is available. Please use this listing as > your means of planning your attendance. > > I would also like to indicate that many aspects of the > web page have been updated, including information concerning > conference sponsors, exhibitors and hospitality suites. > We will continue to add new information as appropriate. > Those who have already registered may wish to check the page > to be sure that they have all the appropriate information. > > Although we can no longer accept abstracts, we are still > accepting registrations. Please consult the web page for > information. > > On behalf of the Organizing Committee, I look forward to > welcoming all the particpants to an exciting meeting in > Keystone. > > Andy Byrd > XVIIth ICMRBS > ------------------ > Dr. R. Andrew Byrd > Macromolecular NMR/MSL/ABL > TEL: 301-846-1407 > FAX: 301-846-6195 Just a note to let you know that SciWeb, a new web site at: http://sciweb.com will list meeting announcements on the site to the Life Science community - announcements go up for free. We also list post doc and technician openings for free and faculty positions for $50/posting. We keep the ad up for two months unless requested otherwise. If you are interested, just write to: eddyjake@sciweb.com and send the text of the post and the contact information. We will get it up within a day for you. SciWeb is a central repository of information and communication services to the life science industry. SciWeb offers free access to useful databases, employment listings, web directories, public discussion groups and data services aiding the life sciences, the pharmaceutical, medical diagnostics and the biotechnology industries. SciWeb provides services for the design of web sites for interested groups and offers low cost computer hosting facilities for secure web sites. For more info, reach us at eddyjake@sciweb.com. Dr. Edward B. Jakobovits, Ph.D. From owner-structural-nmr@net.bio.net Thu Jul 18 23:00:00 1996 Path: biosci!daresbury!is.bbsrc.ac.uk!news.ox.ac.uk!news From: "Simon M. Brocklehurst" Newsgroups: bionet.structural-nmr Subject: NAOMI Software Date: Fri, 19 Jul 1996 12:20:54 +0100 Organization: University of Oxford Lines: 44 Message-ID: <31EF6F96.59E2B600@bioch.ox.ac.uk> NNTP-Posting-Host: nmrv.ocms.ox.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.01 (X11; I; SunOS 4.1.3_U1 sun4m) NAOMI - Message to users/prospective users _____________________________________________________________________________ Due to the large number of requests for NAOMI licenses in the last 2 months, there has been a delay in supplying license keys. This backlog, however, is now cleared. Because of the heavy influx of mail/e-mail, however, I think a few people's requests may have slipped through the net. If you contacted me more than 1 week ago, and have not yet received a reply I'd be grateful if you'd re-send your message. ----------------------------------------------------------------------------- NAOMI - for studying 3-D structures of proteins. NAOMI is available free of charge for academic users. NAOMI Version 2.4c is available as of now from the NAOMI Web site at: http://www.ocms.ox.ac.uk/~smb/Software/N_details/naomi.html or via anonymous ftp ftp://nmrz.ocms.ox.ac.uk/pub/smb/naomi i.e. at nmrz.ocms.ox.ac.uk in directory pub/smb/naomi/ ----------------------------------------------------------------------------- More information at the commercial Web site at: http://www.psynix.co.uk/products/naomi/index.html _____________________________________________________________________________ | | ,_ o Simon M. Brocklehurst, | / //\, Oxford Centre for Molecular Sciences, Department of Biochemistry, | \>> | University of Oxford, Oxford, UK. | \\, E-mail: smb@bioch.ox.ac.uk | WWW: http://www.ocms.ox.ac.uk/~smb/ |____________________________________________________________________________ From owner-structural-nmr@net.bio.net Thu Jul 18 23:00:00 1996 Path: biosci!UCHSC.EDU!Michael.Overduin From: Michael.Overduin@UCHSC.EDU (Michael Overduin) Newsgroups: bionet.structural-nmr Subject: address request Date: 19 Jul 1996 09:36:20 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Could someone e-mail the address of Mike Cummings? Thanks, _________________________________________________________________________ Michael Overduin Assistant Professor (303) 270-8774 (office) Department of Pharmacology, Campus Box C236 (303) 270-7097 (FAX) University of Colorado Health Sciences Center Michael.Overduin@UCHSC.edu 4200 East Ninth Avenue, Denver, CO 80262 http://www.uchsc.edu/sm/pharmaco/faculty/Overduin/MOhome.htm From owner-structural-nmr@net.bio.net Thu Jul 18 23:00:00 1996 Path: biosci!daresbury!nntp-trd.UNINETT.no!newsfeed.sunet.se!news00.sunet.se!sunic!nntp.coast.net!lll-winken.llnl.gov!uwm.edu!usenet From: Hanqing Wu Newsgroups: bionet.structural-nmr Subject: Online EPR Spectrum Simulation through CGIEMAIL Date: Fri, 19 Jul 1996 16:48:27 -0700 Organization: Home Lines: 11 Message-ID: <31F01ECB.2A18@alpha1.csd.uwm.edu> Reply-To: hanqing@alpha1.csd.uwm.edu NNTP-Posting-Host: 129.89.8.201 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0b4Gold (Win95; I; 16bit) CC: hanqing@alpha1.csd.uwm.edu I have created a homepage of "Online EPR Spectrum Simulation through CGIEMAIL" at "http://www.uwm.edu/~hanqing/watoc/oleprsm.htm". Please look the page and give me suggestion. Two electronic posters on EPR simulation presented at WATOC96 can be seen at "http://www.ch.ic.ac.uk/watoc/abstracts/". Thanks! Hanqing Wu hanqing@alpha1.csd.uwm.edu From owner-structural-nmr@net.bio.net Mon Jul 22 23:00:00 1996 Path: biosci!sbphrd.com!Lesley_K_Maclachlan From: Lesley_K_Maclachlan@sbphrd.com (Lesley K Maclachlan) Newsgroups: bionet.structural-nmr Subject: CASE studentship available Date: 23 Jul 1996 08:16:02 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 20 Sender: daemon@net.bio.net Distribution: world Message-ID: <9607231814.AA1664@pho903.sbphrd.com> NNTP-Posting-Host: net.bio.net Applications are invited for a CASE studentship with Dr Jonathon Waltho, Department of Molecular Biology and Biotechnology, University of Sheffield, and SmithKline Beecham Pharmaceuticals on NMR studies of RNA fragments and RNA-peptide complexes, using isotopic labelling methodologies. The studentship commences on the 1st October 1996. Further information about this project and the CASE award may be obtained either from Dr Jon Waltho (telephone: +44-144-2824224; e-mail: j.waltho@sheffield.ac.uk), or from Dr Julia Hubbard (SB) (telephone: +44-1438-78-2007; e-mail: Julia_Hubbard-1@sbphrd.com) or from Dr Lesley MacLachlan (SB) at the address below. ------------------------------------------------------------------------------------------------- Dr L. K. MacLachlan SmithKline Beecham Pharmaceuticals Tel.: +44-1438-78-2007 The Frythe, Welwyn Fax: +44-1438-78-2570 Hertfordshire AL6 9AR E-Mail: Lesley_K_Maclachlan@sbphrd.com United Kingdom The opinions expressed in this communication are my own, and do not necessarily reflect those of my employer. ------------------------------------------------------------------------------------------------- From owner-structural-nmr@net.bio.net Mon Jul 22 23:00:00 1996 Path: biosci!sbphrd.com!Julia_Hubbard-1 From: Julia_Hubbard-1@sbphrd.com (Julia Hubbard-1) Newsgroups: bionet.structural-nmr Subject: subscribe Date: 23 Jul 1996 07:10:34 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: <9607231708.AA0513@pho903.sbphrd.com> NNTP-Posting-Host: net.bio.net subscribe From owner-structural-nmr@net.bio.net Mon Jul 22 23:00:00 1996 Path: biosci!sbphrd.com!Lesley_K_Maclachlan From: Lesley_K_Maclachlan@sbphrd.com (Lesley_K_Maclachlan) Newsgroups: bionet.structural-nmr Subject: CASE studentship available Date: 23 Jul 1996 07:26:45 -0700 Organization: SmithKline Beecham Lines: 20 Sender: daemon@net.bio.net Distribution: world Message-ID: <31F551CF.99E@sbphrd.com> NNTP-Posting-Host: net.bio.net Applications are invited for a CASE studentship with Dr Jonathon Waltho, Department of Molecular Biology and Biotechnology, University of Sheffield, and SmithKline Beecham Pharmaceuticals on NMR studies of RNA fragments and RNA-peptide complexes, using isotopic labelling methodologies. The studentship commences on the 1st October 1996. Further information about this project and the CASE award may be obtained either from Dr Jon Waltho (telephone: +44-144-2824224; e-mail: j.waltho@sheffield.ac.uk), or from Dr Julia Hubbard (SB) (telephone: +44-1438-78-2007; e-mail: Julia_Hubbard-1@sbphrd.com) or from Dr Lesley MacLachlan (SB) at the address below. -------------------------------------------------------------------------Dr L. K. MacLachlan SmithKline Beecham Pharmaceuticals Tel.: +44-1438-78-2007 The Frythe, Welwyn Fax: +44-1438-78-2570 Hertfordshire AL6 9AR E-Mail: Lesley_K_Maclachlan@sbphrd.com United Kingdom The opinions expressed in this communication are my own, and do not necessarily reflect those of my employer. ------------------------------------------------------------------------- From owner-structural-nmr@net.bio.net Mon Jul 22 23:00:00 1996 Path: biosci!agate!newsgate.duke.edu!news-server.ncren.net!news_server.cs.unc.edu!newz.oit.unc.edu!usenet From: "mhs.unc.edu" Newsgroups: bionet.structural-nmr Subject: postdoctoral position(s) Date: 23 Jul 1996 15:59:51 GMT Organization: The University of North Carolina at Chapel Hill Lines: 137 Message-ID: <4t2stn$4i@newz.oit.unc.edu> NNTP-Posting-Host: mac231h530b.med.unc.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.12(Macintosh; I; PPC) To: campbesl@med.unc.edu X-URL: news:bionet.structural-nmr 1-2 Postdoctoral Positions in Biomolecular NMR Starting Date: 11/96-3/97 The general research area involves structure/function studies of onco-proteins and tumor suppressor proteins involved in regulation of cellular growth control. Several projects are devoted to studies of regulatory proteins involved in cell signaling pathways mediated by the low molecular weight guanine nucleotide binding proteins: Ras, Rac and Rho. The projects are being conducted in close collaboration with two forefront molecular and cell biology laboratories with established research programs in these areas. Applicants interested in cancer biology with background in heteronuclear multidimensional NMR and NMR structure determination methodologies are encouraged to apply. We are the primary users of a macromolecular NMR facility that houses a 4-channel 500 MHz Bruker spectrometer equipped with a gradient accessory. A second high field state-of-the-art spectrometer will be purchased this year and the expanded facility will be housed in a newly renovated building in close proximity to the laboratory. In addition to a fully equipped biochemistry/molecular biology lab, a network of Silicon graphics workstations with Biosym, XPLOR, XEASY and other NMR and molecular graphics display software are available. Interested parties should submit a CV, cover letter and at least two letters of recommendation to : Dr. Sharon Campbell Department of Biochemistry and Biophysics 530 FLOB U. of North Carolina at Chapel Hill Chapel Hill, NC 27599-7260 Tel: 919-966-7139 Fax: 919-966-2852 email: campbesl@med.unc.edu Applicants attending the XVIIth ICMRBS conference should contact Sharon Campbell prior to August 13, 1996. Relevant Publications, 1992-present: 1. 15N-Correlated Three Dimensional Relayed NOESY Experiments in Uniformly 15N-labeled Proteins, L. Mueller, S. Campbell-Burk, P. Domaille, J. Magn. Reson. (1992) 96, 408-415. 2. Four Dimensional Triple Resonance NMR Methods for the Assignment of Backbone Nuclei in Proteins, E.D. Laue, W. Boucher, P.J. Domaille and S. Campbell-Burk, J. Am. Chem. Soc. (1992) 114, 2262-2264. 3. Improved 4D NMR Experiments for the Assignment of Backbone Nuclei in 13C/15N Labeled Proteins, W. Boucher, E.D. Laue, S. Campbell-Burk and P. J. Domaille, J. Biomolecular NMR (1992) 2, 631-637. 4. Sequential Assignment of the Backbone Nuclei (1H, 15N, 13C) of H-Ras.GDP Using a Novel 4D NMR Strategy, S.L. Campbell-Burk, P.J. Domaille, M.A. Starovasnik, E.D. Laue and W. Boucher, J. Biomolecular NMR (1992) 2, 639-646. 5. Identification of residues critical for Ras (17N) growth inhibitory phenotype and for Ras interaction with guanine nucleotide exchange factors, L.A. Quilliam, K.Kato, K.M., Rabun, M.M. Hisaka, S. Huff, S. Campbell-Burk, and C.J. Der. Mol. Cell. Biol. (1993) 14, 1113-1121. 6. Analysis of Ras Structure by NMR, S. Campbell-Burk & T. Van Aken, Regulatory GTP Binding Proteins (1994) 213-234, Handbook of Pharmacology, Springer-Verlag. 7. Secondary Structure and Signal Assignments of HIV-1 Protease Complexed to a Novel, Structure Based Inhibitor, T. Yamazaki, L.K. Nicholson, D.A. Torchia, S.J. Stahl, J.D. Kaufman, P.T. Wingfield, P.J. Domaille, S. Campbell-Burk, P. Y-S. Lam, Eur. J. Biochem. (1994), 219, 707-712. 8. The Solution Structure and Dynamics of Ras p21.GDP Determined by Heteronuclear Three and Four Dimensional NMR Spectroscopy, P.J. Kraulis, P.J. Domaille, S.L. Campbell-Burk, T.A. Van Aken, E.D. Laue, Biochemistry (1994) 33, 3515-3531. 9. Refolding and Purification of H-Ras p21 from Inclusion Bodies, R. DeLoskey, T. Van Aken and S. Campbell-Burk, Archives of Biochemistry and Biophysics (1994) 311, 72-78. 10. Biomolecular Applications of Heteronuclear NMR, S.L. Campbell-Burk & S. Zhong, Current Topics in Biotechnology (1994) 5, 346-354. 11. Mutation of Phenylalanine 156, a Residue Conserved in All Members of the Ras Superfamily, Causes Structural Rearrangement of Ha-Ras and Induces Cellular Transformation. L.A. Quilliam, S. Zhong, K.M. Rabun, J. Carpenter, C.J. Der and S.L. Campbell-Burk (1995) PNAS 92, 1272-1276. 12. Expression and Purification of Bacterially-Expressed Ras Protein, S.L. Campbell-Burk and J. Carpenter, in Small GTPases and Their Regulators:Part A: Ras Family, Methods in Enzymology, (1995) 255, 3-13. 13. Two Distinct Ras Domains Mediate Interaction with Ras. T.R. Brtva, J.K. Drugan, S. Ghosh, R.S. Terrell, S. Campbell, R.M. Bell and C.J. Der, Journal of Biological Chemistry, (1995) 270, 9809-9812. 14. Inhibition of Ras Function by Peptides Containing a Consensus Ras Binding Sequence from Raf-1 and NF1, Geoffrey J. Clark, Jonelle K. Drugan, Regina S. Terrell, Cynthia Bradham, Channing J. Der, Robert M. Bell and Sharon Campbell, PNAS (1996) 93, 1577-1581. 15. Involvement of the Switch 2 Domain of Ras in its Interaction with Guanine Nucleotide Exchange Factors. L.A. Quilliam, M.M. Hisaka, S. Zhong, A. Lowry, R. Mosteller, J. Han, J. Drugan, D. Broek, S. Campbell and C.J. Der. Journal of Biological Chemistry, (1996) 271, 11076-1182. 16. Two Distinct Ras-Binding Sites of Raf are Essential for Ras-Mediated Transformation. J. K. Drugan, R. Kosravi-Far, M. White, Y-W. Hwang, C.J. Der and S.L. Campbell. Journal of Biological Chemistry, (1996) 271, 233-267. 17. The NMR Solution Structure of the Raf-1 Cysteine Rich Domain. H. Mott, J. Carpenter, S. Ghosh, B. Bell and Sharon Campbell, PNAS, (in press). 18. Redox Regulation of Cell Signalling. H.M. Lander, A.J. Milbank, J.M. Tauras, D.P. Hajjar, B.L. Hempstead, G.D. Schwartz, R.T. Kraemer, U.A. Mirza, B.T. Chait, S. Campbell and L.A. Quilliam. Nature, (1996) 381, 380-381. 19. A Molecular Redox Switch on p21ras: Structural Basis for the Nitric Oxide-p21ras Interaction. H.M. Lander, A.J. Milbank, J.M. Tauras, D.P. Hajjar, B.L. Hempstead, G.D. Schwartz, R.T. Kraemer, U.A. Mirza, B.T. Chait, S. Campbell and L.A. Quilliam. (submitted). 20. Ras:Effector Mediated Signaling Pathways. R. Khosravi-Far, S. Campbell and C.J. Der. Oncogene {invited review, in press}. 21. Dbl Family Proteins. I.P. Whitehead, S. Campbell, K.L. Rossman and C.J. Der. BBA Reviews in Cancer {invited review, in press}. From owner-structural-nmr@net.bio.net Mon Jul 22 23:00:00 1996 Path: biosci!agate!newsgate.duke.edu!news-server.ncren.net!news_server.cs.unc.edu!newz.oit.unc.edu!usenet From: "mhs.unc.edu" Newsgroups: bionet.structural-nmr Subject: postdoctoral position(s) Date: 23 Jul 1996 16:27:45 GMT Organization: The University of North Carolina at Chapel Hill Lines: 141 Message-ID: <4t2ui1$4i@newz.oit.unc.edu> NNTP-Posting-Host: mac231h530b.med.unc.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: quoted-printable X-Mailer: Mozilla 1.12(Macintosh; I; PPC) X-URL: news:bionet.structural-nmr Postdoctoral Positions in Biomolecular NMR Starting Date: 11/96-3/97 The general research area involves structure/function studies of onco-proteins and tumor suppressor proteins involved in regulation of cellular growth control. Several projects are devoted to studies of regulatory proteins involved in cell signaling pathways mediated by the low molecular weight guanine nucleotide binding proteins: Ras, Rac and Rho. The projects are being conducted in close collaboration with two forefront molecular and cell biology laboratories with established research programs in these areas. Applicants interested in cancer biology with background in heteronuclear multidimensional NMR and NMR structure d=1Betermination methodologies are encouraged to apply. We are the primary users of a macromolecular NMR facility that houses a 4-channel 500 MHz Bruker spectrometer equipped with a gradient accessory. A second high field state-of-the-art spectrometer will be purchased this year and the expanded facility will be housed in a newly renovated building in close proximity to the laboratory. In addition to a fully equipped biochemistry/molecular biology lab, a network of Silicon graphics workstations with Biosym, XPLOR, XEASY and other NMR and molecular g=1Braphics display software are available. Interested parties should submit a=1B CV, cover letter and at least two letters of recommendation to : Dr. Sharon Campbell Department of Biochemistry and Biophysics 530 FLOB U. of North Carolina at Chapel Hill Chapel Hill, NC 27599-7260 Tel: 919-966-7139 Fax: 919-966-2852 email: campbesl@med.unc.edu Applicants attending the XVIIth ICMRBS conference should contact Sharon Campbell prior to August 13, 1996. Relevant Publications, 1992-present: 1. 15N-Correlated Three Dimensional Relayed NOESY Experiments in Uniformly 15N-labeled Proteins, L. Mueller, S. Campbell-Burk, P. Domaille, J. Magn. Reson. (1992) 96, 408-415. 2. Four Dimensional Triple Resonance NMR Methods for the Assignment of Backbone Nuclei in Proteins, E.D. Laue, W. Boucher, P.J. Domaille and S. Campbell-Burk, J. Am. Chem. Soc. (1992) 114, 2262-2264. 3. Improved 4D NMR Experiments for the Assignment of Backbone Nuclei in 13C/15N Labeled Proteins, W. Boucher, E.D. Laue, S. Campbell-Burk and P. J. Domaille, J. Biomolecular NMR (1992) 2, 631-637. 4. Sequential Assignment of the Backbone Nuclei (1H, 15N, 13C) of H-Ras.GDP Using a Novel 4D NMR Strategy, S.L. Campbell-Burk, P.J. Domaille, M.A. Starovasnik, E.D. Laue and W. Boucher, J. Biomolecular NMR (1992) 2, 639-646. 5. Identification of residues critical for Ras (17N) growth inhibitory phenotype and for Ras interaction with guanine nucleotide exchange factors, L.A. Quilliam, K.Kato, K.M., Rabun, M.M. Hisaka, S. Huff, S. Campbell-Burk, and C.J. Der. Mol. Cell. Biol. (1993) 14, 1113-1121. 6. Analysis of Ras Structure by NMR, S. Campbell-Burk & T. Van Aken, Regulatory GTP Binding Proteins (1994) 213-234, Handbook of Pharmacology, Springer-Verlag. 7. Secondary Structure and Signal Assignments of HIV-1 Protease Complexed to a Novel, Structure Based Inhibitor, T. Yamazaki, L.K. Nicholson, D.A. Torchia, S.J. Stahl, J.D. Kaufman, P.T. Wingfield, P.J. Domaille, S. Campbell-Burk, P. Y-S. Lam, Eur. J. Biochem. (1994), 219, 707-712. 8. The Solution Structure and Dynamics of Ras p21.GDP Determined by Heteronuclear Three and Four Dimensional NMR Spectroscopy, P.J. Kraulis, P.J. Domaille, S.L. Campbell-Burk, T.A. Van Aken, E.D. Laue, Biochemistry (1994) 33, 3515-3531. 9. Refolding and Purification of H-Ras p21 from Inclusion Bodies, R. DeLoskey, T. Van Aken and S. Campbell-Burk, Archives of Biochemistry and Biophysics (1994) 311, 72-78. 10. Biomolecular Applications of Heteronuclear NMR, S.L. Campbell-Burk & S. Zhong, Current Topics in Biotechnology (1994) 5, 346-354. 11. Mutation of Phenylalanine 156, a Residue Conserved in All Members of the Ras Superfamily, Causes Structural Rearrangement of Ha-Ras and Induces Cellular Transformation. L.A. Quilliam, S. Zhong, K.M. Rabun, J. Carpenter, C.J. Der and S.L. Campbell-Burk (1995) PNAS 92, 1272-1276. 12. Expression and Purification of Bacterially-Expressed Ras Protein, S.L. Campbell-Burk and J. Carpenter, in Small GTPases and Their Regulators:Part A: Ras Family, Methods in Enzymology, (1995) 255, 3-13. 13. Two Distinct Ras Domains Mediate Interaction with Ras. T.R. Brtva, J.K. Drugan, S. Ghosh, R.S. Terrell, S. Campbell, R.M. Bell and C.J. Der, Journal of Biological Chemistry, (1995) 270, 9809-9812. 14. Inhibition of Ras Function by Peptides Containing a Consensus Ras Binding Sequence from Raf-1 and NF1, Geoffrey J. Clark, Jonelle K. Drugan, Regina S. Terrell, Cynthia Bradham, Channing J. Der, Robert M. Bell and Sharon Campbell, PNAS (1996) 93, 1577-1581. 15. Involvement of the Switch 2 Domain of Ras in its Interaction with Guanine Nucleotide Exchange Factors. L.A. Quilliam, M.M. Hisaka, S. Zhong, A. Lowry, R. Mosteller, J. Han, J. Drugan, D. Broek, S. Campbell and C.J. Der. Journal of Biological Chemistry, (1996) 271, 11076-1182. 16. Two Distinct Ras-Binding Sites of Raf are Essential for Ras-Mediated Transformation. J. K. Drugan, R. Kosravi-Far, M. White, Y-W. Hwang, C.J. Der and S.L. Campbell. Journal of Biological Chemistry, (1996) 271, 233-267. 17. The NMR Solution Structure of the Raf-1 Cysteine Rich Domain. H. Mott, J. Carpenter, S. Ghosh, B. Bell and Sharon Campbell, PNAS, (in press). 18. Redox Regulation of Cell Signalling. H.M. Lander, A.J. Milbank, J.M. Tauras, D.P. Hajjar, B.L. Hempstead, G.D. Schwartz, R.T. Kraemer, U.A. Mirza, B.T. Chait, S. Campbell and L.A. Quilliam. Nature, (1996) 381, 380-381. 19. A Molecular Redox Switch on p21ras: Structural Basis for the Nitric Oxide-p21ras Interaction. H.M. Lander, A.J. Milbank, J.M. Tauras, D.P. Hajjar, B.L. Hempstead, G.D. Schwartz, R.T. Kraemer, U.A. Mirza, B.T. Chait, S. Campbell and L.A. Quilliam. (submitted). 20. Ras:Effector Mediated Signaling Pathways. R. Khosravi-Far, S. Campbell and C.J. Der. Oncogene {invited review, in press}. 21. Dbl Family Proteins. I.P. Whitehead, S. Campbell, K.L. Rossman and C.J. Der. BBA Reviews in Cancer {invited review, in press}. From owner-structural-nmr@net.bio.net Mon Jul 22 23:00:00 1996 Path: biosci!agate!spool.mu.edu!howland.reston.ans.net!EU.net!usenet2.news.uk.psi.net!uknet!usenet1.news.uk.psi.net!uknet!uknet!bhamcs!news.ox.ac.uk!nmrakd.ocms!kristy From: kristy@bioch.ox.ac.uk (Kristy Downing) Newsgroups: bionet.structural-nmr Subject: CS based structure refinement Date: 23 Jul 1996 17:45:20 GMT Organization: Oxford University Lines: 13 Message-ID: <4t333g$nke@news.ox.ac.uk> NNTP-Posting-Host: nmrakd.ocms.ox.ac.uk Keywords: chemical shift, structure refinement, X-PLOR Does anyone have a routine for chemical shift based refinement of structures in X-PLOR that they would be willing to share? Many thanks, Kristy Downing -- Dr. A. Kristina Downing e-mail: kristy@bioch.ox.ac.uk University of Oxford Oxford Centre for Molecular Sciences fax. +44 (0) 1865-275253 New Chemistry Laboratory tel. +44 (0) 1865-275956 South Parks Road, Oxford OX1 3QT ENGLAND From owner-structural-nmr@net.bio.net Mon Jul 22 23:00:00 1996 Path: biosci!agate!news.ucdavis.edu!info.ucla.edu!newshub.sdsu.edu!newsfeeder.sdsu.edu!in-news.erinet.com!imci5!imci4!newsfeed.internetmci.com!news.ycc.yale.edu!news From: Greg Warren Newsgroups: bionet.structural-nmr,bionet.software.x-plor Subject: Re: CS based structure refinement Date: Tue, 23 Jul 1996 16:58:08 -0400 Organization: Yale University Lines: 45 Message-ID: <31F53CE0.41C6@laplace.csb.yale.edu> References: <4t333g$nke@news.ox.ac.uk> NNTP-Posting-Host: hamilton.csb.yale.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0b5a (X11; I; IRIX 5.3 IP22) Xref: biosci bionet.structural-nmr:1395 bionet.software.x-plor:634 Kristy Downing wrote: > > Does anyone have a routine for chemical shift based refinement of > structures in X-PLOR that they would be willing to share? > > Many thanks, > > Kristy Downing > > -- > Dr. A. Kristina Downing e-mail: kristy@bioch.ox.ac.uk > University of Oxford > Oxford Centre for Molecular Sciences fax. +44 (0) 1865-275253 > New Chemistry Laboratory tel. +44 (0) 1865-275956 > South Parks Road, Oxford OX1 3QT ENGLAND Kristy, Chemical shift refinement (C13 or proton) will be in the developmental version of XPLOR thanks for Jon Kuszewski. This version will be available to licensed users in two or three weeks via ftp. For more information check the web site at http://xplor.csb.yale.edu/xplor-info/xplor-info.html We will be sending an announcement to both the structural-nmr and software.x-plor newsgroups when this version of XPLOR becomes accessible. Greg Warren -- =========================================================== Gregory L. Warren, PhD Department of Molecular Biophysics and Biochemistry Yale University 266 Whitney Avenue New Haven, CT 06520-8114 USA (203) 432-5066 (203) 432-3923 - fax gwarren@laplace.csb.yale.edu Home address: 91 Bishop St. Apt 11 New Haven, CT 06511 =========================================================== From owner-structural-nmr@net.bio.net Mon Jul 22 23:00:00 1996 Path: biosci!chula.ac.th!gc685059 From: gc685059@chula.ac.th (Nopporn Wichailak) Newsgroups: bionet.structural-nmr Subject: (none) Date: 23 Jul 1996 11:59:21 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net unsubscribe From owner-structural-nmr@net.bio.net Tue Jul 23 23:00:00 1996 Path: biosci!LAPLACE.CSB.YALE.EDU!gwarren From: gwarren@LAPLACE.CSB.YALE.EDU (Greg Warren) Newsgroups: bionet.structural-nmr Subject: Re: CS based structure refinement Date: 24 Jul 1996 10:49:54 -0700 Organization: Yale University Lines: 47 Sender: daemon@net.bio.net Distribution: world Message-ID: <31F6624D.446B@laplace.csb.yale.edu> References: NNTP-Posting-Host: net.bio.net Kegl, At the present time there is no functional port to Linux, either supported or unsupported. Greg Kegl Tamas wrote: > > On Tue, 23 Jul 1996, Greg Warren wrote: > > > Chemical shift refinement (C13 or proton) will be in the developmental > > version of XPLOR thanks for Jon Kuszewski. This version will be > > available to licensed users in two or three weeks via ftp. For more > > information check the web site at > > > > http://xplor.csb.yale.edu/xplor-info/xplor-info.html > > Is it possible to compile X-PLOR on PC/Linux systems? As I saw the help-page > here are plattform-dependent parts in the code as well. > > Thanks, > Tamas > > ############################################################################## > Tamas Kegl | ** ** > Ph.D. student | * * * > University of Veszprem | * * <-- Linux Inside!!! > Department of Organic Chemistry | * * > ---------------------------------- | * * HuLUG > E-mail: monty@python.mars.vein.hu | * ^^^^^ > ############################################################################## -- =========================================================== Gregory L. Warren, PhD Department of Molecular Biophysics and Biochemistry Yale University 266 Whitney Avenue New Haven, CT 06520-8114 USA (203) 432-5066 (203) 432-3923 - fax gwarren@laplace.csb.yale.edu Home address: 91 Bishop St. Apt 11 New Haven, CT 06511 =========================================================== From owner-structural-nmr@net.bio.net Tue Jul 23 23:00:00 1996 Path: biosci!PYTHON.MARS.VEIN.HU!monty From: monty@PYTHON.MARS.VEIN.HU (Kegl Tamas) Newsgroups: bionet.structural-nmr Subject: Re: CS based structure refinement Date: 24 Jul 1996 02:46:12 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 23 Sender: daemon@net.bio.net Distribution: world Message-ID: Reply-To: monty@python.mars.vein.hu NNTP-Posting-Host: net.bio.net On Tue, 23 Jul 1996, Greg Warren wrote: > Chemical shift refinement (C13 or proton) will be in the developmental > version of XPLOR thanks for Jon Kuszewski. This version will be > available to licensed users in two or three weeks via ftp. For more > information check the web site at > > http://xplor.csb.yale.edu/xplor-info/xplor-info.html Is it possible to compile X-PLOR on PC/Linux systems? As I saw the help-page here are plattform-dependent parts in the code as well. Thanks, Tamas ############################################################################## Tamas Kegl | ** ** Ph.D. student | * * * University of Veszprem | * * <-- Linux Inside!!! Department of Organic Chemistry | * * ---------------------------------- | * * HuLUG E-mail: monty@python.mars.vein.hu | * ^^^^^ ############################################################################## From owner-structural-nmr@net.bio.net Wed Jul 24 23:00:00 1996 Path: biosci!bloom-beacon.mit.edu!spool.mu.edu!uwm.edu!vixen.cso.uiuc.edu!newsfeed.internetmci.com!in2.uu.net!news2.digex.net!usenet From: numare@cnj.digex.net Newsgroups: bionet.structural-nmr Subject: Re: News Release Date: Thu, 25 Jul 96 13:13:53 PDT Organization: Express Access Online Communications, USA Lines: 16 Message-ID: References: <960725113551_584552812@emout13.mail.aol.com> NNTP-Posting-Host: cjc08014.slip.digex.net Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII X-Newsreader: NEWTNews & Chameleon -- TCP/IP for MS Windows from NetManage > The objective of the M R Resources sales group is to listen to and understand > the needs of its customers, and recommend the appropriate products and > services which will address those needs in a cost effective manner. Lawrence, > who is an experienced professional in NMR sales, brings to M R Resources a > keen ability to develop long term relationships with his customers. > Relationship building is a critical piece of M R Resources’ strategy. It > allows its salespeople to quickly and thoroughly understand their customer’s > situations and immediately respond with the proper solution. > BS From owner-structural-nmr@net.bio.net Wed Jul 24 23:00:00 1996 Path: biosci!MRR.COM!jon From: jon@MRR.COM (Jon Webb) Newsgroups: bionet.structural-nmr Subject: change of address Date: 25 Jul 1996 13:14:40 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 26 Sender: daemon@net.bio.net Distribution: world Message-ID: <2.2.32.19960725201141.006f84c4@205.198.86.145> NNTP-Posting-Host: net.bio.net Please forward messages from this newsgroup to my new email address which is: jon@mrr.com Best regards, Jon ____________________________________________________________________________ ________ Jon Webb M R Resources, Inc Remanufactured NMR & MRI Systems, Parts and Services 158 R. Main Street P.O. Box 880 Gardner, MA 01440 Voice: 508-632-7000 Fax: 508-630-2509 Email: jon@mrr.com Web Site: http://www.mrr.com ____________________________________________________________________________ ________ From owner-structural-nmr@net.bio.net Wed Jul 24 23:00:00 1996 Path: biosci!aol.com!Resourcemr From: Resourcemr@aol.com Newsgroups: bionet.structural-nmr Subject: News Release Date: 25 Jul 1996 08:36:30 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 31 Sender: daemon@net.bio.net Distribution: world Message-ID: <960725113551_584552812@emout13.mail.aol.com> NNTP-Posting-Host: net.bio.net FOR IMMEDIATE RELEASE M R Resources, the leading supplier of remanufactured NMR systems, services, and accessories is pleased to announce the addition of Lawrence Bennett to its sales and marketing group. Lawrence joins M R Resources as product line specialist, and will focus his activities on sales of NMR field services, accessories and parts for most major models of NMR spectrometers. Specifically, this product line includes field repair and maintenance of NMR systems, service contracts, cryogen filling services, probes, sample changers, disk drives, spectrometer upgrades, and repair parts. The objective of the M R Resources sales group is to listen to and understand the needs of its customers, and recommend the appropriate products and services which will address those needs in a cost effective manner. Lawrence, who is an experienced professional in NMR sales, brings to M R Resources a keen ability to develop long term relationships with his customers. Relationship building is a critical piece of M R Resources’ strategy. It allows its salespeople to quickly and thoroughly understand their customer’s situations and immediately respond with the proper solution. Bennett, the former sales manager for Doty Scientific, was given a hearty welcome at the M R Resources facility this past Monday. Bennett says "I am looking forward to working with my NMR colleagues to provide them with the highest quality products and services". More about M R Resources at its web site located at: http://www.mrr.com -30- From owner-structural-nmr@net.bio.net Thu Jul 25 23:00:00 1996 Path: biosci!rutgers!uwm.edu!news.cse.psu.edu!news.cc.swarthmore.edu!netnews.upenn.edu!dsinc!newsfeed.pitt.edu!godot.cc.duq.edu!newsgate.duke.edu!news-server.ncren.net!concert!newz.oit.unc.edu!usenet From: "mhs.unc.edu" Newsgroups: bionet.structural-nmr Subject: postdoctoral position Date: 26 Jul 1996 21:08:41 GMT Organization: The University of North Carolina at Chapel Hill Lines: 170 Message-ID: <4tbc4p$sp2@newz.oit.unc.edu> NNTP-Posting-Host: mac231h530b.med.unc.edu Mime-Version: 1.0 Content-Type: multipart/mixed; boundary="-------------------------------323181489111785" X-Mailer: Mozilla 1.12(Macintosh; I; PPC) X-URL: news:bionet.structural-nmr This is a multi-part message in MIME format. ---------------------------------323181489111785 Content-Transfer-Encoding: 7bit Content-Type: text/plain; charset=us-ascii 1-2 Postdoctoral Positions in Biomolecular NMR Starting Date: 11/96-3/97 The general research area involves structure/function studies of onco-proteins and tumor suppressor proteins involved in regulation of cellular growth control. Several projects are devoted to studies of regulatory proteins involved in cell signaling pathways mediated by the low molecular weight guanine nucleotide binding proteins: Ras, Rac and Rho. The projects are being conducted in close collaboration with two forefront molecular and cell biology laboratories with established research programs in these areas. Applicants interested in cancer biology with background in heteronuclear multidimensional NMR and NMR structure determination methodologies are encouraged to apply. We are the primary users of a macromolecular NMR facility that houses a 4-channel 500 MHz Bruker spectrometer equipped with a gradient accessory. A second high field state-of-the-art spectrometer will be purchased this year and the expanded facility will be housed in a newly renovated building in close proximity to the laboratory. In addition to a fully equipped biochemistry/molecular biology lab, a network of Silicon graphics workstations with Biosym, XPLOR, XEASY and other NMR and molecular graphics display software available. Interested parties should submit a CV, cover letter and at least two letters of recommendation to : Dr. Sharon Campbell Department of Biochemistry and Biophysics 530 FLOB U. of North Carolina at Chapel Hill Chapel Hill, NC 27599-7260 Tel: 919-966-7139 Fax: 919-966-2852 email: campbesl@med.unc.edu Applicants attending the XVIIth ICMRBS conference should contact Sharon Campbell prior to August 13, 1996. Relevant Publications, 1992-present: 1. 15N-Correlated Three Dimensional Relayed NOESY Experiments in Uniformly 15N-labeled Proteins, L. Mueller, S. Campbell-Burk, P. Domaille, J. Magn. Reson. (1992) 96, 408-415. 2. Four Dimensional Triple Resonance NMR Methods for the Assignment of Backbone Nuclei in Proteins, E.D. Laue, W. Boucher, P.J. Domaille and S. Campbell-Burk, J. Am. Chem. Soc. (1992) 114, 2262-2264. 3. Improved 4D NMR Experiments for the Assignment of Backbone Nuclei in 13C/15N Labeled Proteins, W. Boucher, E.D. Laue, S. Campbell-Burk and P. J. Domaille, J. Biomolecular NMR (1992) 2, 631-637. 4. Sequential Assignment of the Backbone Nuclei (1H, 15N, 13C) of H-Ras.GDP Using a Novel 4D NMR Strategy, S.L. Campbell-Burk, P.J. Domaille, M.A. Starovasnik, E.D. Laue and W. Boucher, J. Biomolecular NMR (1992) 2, 639-646. 5. Identification of residues critical for Ras (17N) growth inhibitory phenotype and for Ras interaction with guanine nucleotide exchange factors, L.A. Quilliam, K.Kato, K.M., Rabun, M.M. Hisaka, S. Huff, S. Campbell-Burk, and C.J. Der. Mol. Cell. Biol. (1993) 14, 1113-1121. 6. Analysis of Ras Structure by NMR, S. Campbell-Burk & T. Van Aken, Regulatory GTP Binding Proteins (1994) 213-234, Handbook of Pharmacology, Springer-Verlag. 7. Secondary Structure and Signal Assignments of HIV-1 Protease Complexed to a Novel, Structure Based Inhibitor, T. Yamazaki, L.K. Nicholson, D.A. Torchia, S.J. Stahl, J.D. Kaufman, P.T. Wingfield, P.J. Domaille, S. Campbell-Burk, P. Y-S. Lam, Eur. J. Biochem. (1994), 219, 707-712. 8. The Solution Structure and Dynamics of Ras p21.GDP Determined by Heteronuclear Three and Four Dimensional NMR Spectroscopy, P.J. Kraulis, P.J. Domaille, S.L. Campbell-Burk, T.A. Van Aken, E.D. Laue, Biochemistry (1994) 33, 3515-3531. 9. Refolding and Purification of H-Ras p21 from Inclusion Bodies, R. DeLoskey, T. Van Aken and S. Campbell-Burk, Archives of Biochemistry and Biophysics (1994) 311, 72-78. 10. Biomolecular Applications of Heteronuclear NMR, S.L. Campbell-Burk & S. Zhong, Current Topics in Biotechnology (1994) 5, 346-354. 11. Mutation of Phenylalanine 156, a Residue Conserved in All Members of the Ras Superfamily, Causes Structural Rearrangement of Ha-Ras and Induces Cellular Transformation. L.A. Quilliam, S. Zhong, K.M. Rabun, J. Carpenter, C.J. Der and S.L. Campbell-Burk (1995) PNAS 92, 1272-1276. 12. Expression and Purification of Bacterially-Expressed Ras Protein, S.L. Campbell-Burk and J. Carpenter, in Small GTPases and Their Regulators:Part A: Ras Family, Methods in Enzymology, (1995) 255, 3-13. 13. Two Distinct Ras Domains Mediate Interaction with Ras. T.R. Brtva, J.K. Drugan, S. Ghosh, R.S. Terrell, S. Campbell, R.M. Bell and C.J. Der, Journal of Biological Chemistry, (1995) 270, 9809-9812. 14. Inhibition of Ras Function by Peptides Containing a Consensus Ras Binding Sequence from Raf-1 and NF1, Geoffrey J. Clark, Jonelle K. Drugan, Regina S. Terrell, Cynthia Bradham, Channing J. Der, Robert M. Bell and Sharon Campbell, PNAS (1996) 93, 1577-1581. 15. Involvement of the Switch 2 Domain of Ras in its Interaction with Guanine Nucleotide Exchange Factors. L.A. Quilliam, M.M. Hisaka, S. Zhong, A. Lowry, R. Mosteller, J. Han, J. Drugan, D. Broek, S. Campbell and C.J. Der. Journal of Biological Chemistry, (1996) 271, 11076-1182. 16. Two Distinct Ras-Binding Sites of Raf are Essential for Ras-Mediated Transformation. J. K. Drugan, R. Kosravi-Far, M. White, Y-W. Hwang, C.J. Der and S.L. Campbell. Journal of Biological Chemistry, (1996) 271, 233-267. 17. The NMR Solution Structure of the Raf-1 Cysteine Rich Domain. H. Mott, J. Carpenter, S. Ghosh, B. Bell and Sharon Campbell, PNAS, (in press). 18. Redox Regulation of Cell Signalling. H.M. Lander, A.J. Milbank, J.M. Tauras, D.P. Hajjar, B.L. Hempstead, G.D. Schwartz, R.T. Kraemer, U.A. Mirza, B.T. Chait, S. Campbell and L.A. Quilliam. Nature, (1996) 381, 380-381. 19. A Molecular Redox Switch on p21ras: Structural Basis for the Nitric Oxide-p21ras Interaction. H.M. Lander, A.J. Milbank, J.M. Tauras, D.P. Hajjar, B.L. Hempstead, G.D. Schwartz, R.T. Kraemer, U.A. Mirza, B.T. Chait, S. Campbell and L.A. Quilliam. (submitted). 20. Ras:Effector Mediated Signaling Pathways. R. Khosravi-Far, S. Campbell and C.J. Der. Oncogene {invited review, in press}. 21. Dbl Family Proteins. I.P. Whitehead, S. Campbell, K.L. Rossman and C.J. Der. BBA Reviews in Cancer {invited review, in press}. ---------------------------------323181489111785 Content-Transfer-Encoding: 7bit Content-Type: text/plain; charset=iso-8859-1 ---------------------------------------------------------------------------- Newsgroup: bionet.structural-nmr * FIRST ELECTRONIC MOLECULAR GRAPHICS AND MODELLING SOCIETY CONFERENCE - Dennis Sprous (316) * Used MR Equipment Newsletter/site - Gary Turner (34) * Re:Shigemi NMR tubes - uzawa@RIKENNMR.RIKEN.GO.JP (22) * Problem with solvent TFE - Dietmar Kaiser (19) * re: Problem with solvent TFE - Xialing Huang (21) * NAOMI Software - "Simon M. Brocklehurst" (44) * address request - Michael Overduin (11) * Online EPR Spectrum Simulation through CGIEMAIL - Hanqing Wu (11) * CS based structure refinement - Kristy Downing (13) o Greg Warren (45) * (none) - Nopporn Wichailak (2) * Re: CS based structure refinement - Kegl Tamas (23) o Greg Warren (47) * News Release - Resourcemr@aol.com (31) o numare@cnj.digex.net (16) * change of address - Jon Webb (26) ---------------------------------------------------------------------------- ---------------------------------323181489111785-- From owner-structural-nmr@net.bio.net Fri Jul 26 23:00:00 1996 Path: biosci!aecom.yu.edu!girvin From: girvin@aecom.yu.edu (Mark Girvin) Newsgroups: bionet.structural-nmr Subject: NMR Staff Position Open Date: 27 Jul 1996 09:56:33 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 38 Sender: daemon@net.bio.net Distribution: world Message-ID: <199607271642.MAA14915@medusa.bioc.aecom.yu.edu> NNTP-Posting-Host: net.bio.net NMR STAFF POSITION NMR staff position to oversee new 600 and 300 MHz Bruker spectrometers and SGI UNIX workstations, implement pulse sequences, and collaborate with and assist users. The new Structural NMR Resource at Albert Einstein College of Medicine (AECOM) occupies a space constructed to house the new Bruker DRX-600 4-channel and DRX-300 2-channel systems, along with substantial computational resources (hardware & software). The new Resource complements the existing 500, 400, and 200 MHz instruments which are used for physiological NMR. AECOM provides an interactive, collaborative, friendly, and stimulating environment. The Biochemistry Department is particularly strong in mechanistic and structural biochemistry. Perhaps surprisingly, the Albert Einstein College of Medicine campus is located in a "pleasant residential neighborhood" of the North Bronx. Many faculty and staff live in the surrounding neighborhoods. Others live in Manhattan, or in Westchester County, which is an easy 10-30 minute commute from AECOM, depending on the location. For the staff position, experience in multidimensional NMR is essential. UNIX software and NMR hardware experience is desirable. The preferred starting date is mid-September to early-October. Please send Curriculum Vitae along with the names, addresses, phone numbers of three references to: Mark Girvin, Biochemistry Department, Albert Einstein College of Medicine, Jack and Pearl Resnick Campus, 1300 Morris Park Ave., Bronx, NY 10461 (girvin@aecom.yu.edu). AECOM is an Equal Opportunity Employer. ______________________________________________________________ Mark Girvin Biochemistry Department Albert Einstein College of Medicine girvin@aecom.yu.edu 1300 Morris Park Ave. Tel:(718) 430-2025/2021 Bronx, NY 10461 FAX:(718) 430-8565 ______________________________________________________________ From owner-structural-nmr@net.bio.net Fri Jul 26 23:00:00 1996 Path: biosci!biosci!not-for-mail From: Iosif Vaisman Newsgroups: bionet.biophysics,bionet.molbio.proteins,bionet.molec-model,bionet.molbio.methds-reagnts,bionet.software,bionet.structural-nmr,bionet.xtallography Subject: Computational Molecular Biology Workshop, October 15-19, 1996 Date: 26 Jul 1996 19:20:59 -0700 Organization: The University of North Carolina at Chapel Hill Lines: 51 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Xref: biosci bionet.biophysics:2176 bionet.molbio.proteins:8396 bionet.molec-model:1079 bionet.molbio.methds-reagnts:47355 bionet.software:16182 bionet.structural-nmr:1403 bionet.xtallography:2764 CAROLINA WORKSHOPS University of North Carolina at Chapel Hill Computational Molecular Biology October 15 - 19, 1996 This course is designed for scientists with limited prior experience in computational molecular biology. The topics to be covered include: biomolecular informatics and databases, protein and nucleic acid sequence analysis and alignment, 3D protein structure analysis and prediction, molecular modeling and dynamic simulations of proteins and nucleic acids, and structure-based drug design. The workshop will consist of the in-depth theoretical lectures and intensive hands-on laboratory sessions. CAROLINA WORKSHOPS are intensive hands-on courses designed to teach cutting edge methods in molecular biology and biotechnology. Four or five courses on different topics in molecular biology and/or biotechnology are offered each year. The courses are designed for novice students as well as for individuals with prior experience. All students benefit from in-depth interaction with instructors. To apply, send a curriculum vitae and a brief letter describing your research interests and their relevance to the Workshop. Applicants should contact the program office as soon as possible. Please indicate your complete mailing address and telephone/fax number. Application Deadline-September 7, 1996. Tuition - $ 1,200.00. Participation is limited to 15 people, please apply early. COURSE DIRECTOR: Alexander Tropsha, Ph.D., University of North Carolina at Chapel Hill INSTRUCTORS: Frank K. Brown (Glaxo-Wellcome) Alexander Tropsha (UNC-Chapel Hill) Wayne Litaker (UNC-Chapel Hill) Iosif I. Vaisman (UNC-Chapel Hill) David C. Richardson (Duke University) For further information or to apply, contact: Dr. Wayne Litaker, Facility Director University of North Carolina at Chapel Hill Program in Molecular Biology & Biotechnology 442 Taylor Hall CB 7100 Chapel Hill, North Carolina 27599-7100 TELEPHONE (919) 966-1730, FAX (919) 966-6821 E-MAIL litaker@med.unc.edu From owner-structural-nmr@net.bio.net Sat Jul 27 23:00:00 1996 Path: biosci!agate!spool.mu.edu!uwm.edu!cs.utexas.edu!swrinde!news-res.gsl.net!news.gsl.net!hunter.premier.net!news.cais.net!newshub.sdsu.edu!newsfeeder.sdsu.edu!newspump.sol.net!news.mindspring.com!usenet From: kindgus@bham.mindspring.com (Matthew Parker) Newsgroups: bionet.structural-nmr,bionet.molbio.proteins,bionet.microbiology,bionet.molbio.methds-reagnts Subject: 13C source for protein NMR Date: Sun, 28 Jul 1996 17:10:53 GMT Organization: MindSpring Enterprises Lines: 19 Message-ID: <4tgau4$2kru@mule0.mindspring.com> Reply-To: kindgus@bham.mindspring.com NNTP-Posting-Host: user-168-121-201-176.dialup.mindspring.com X-Newsreader: Forte Free Agent 1.0.82 Xref: biosci bionet.structural-nmr:1406 bionet.molbio.proteins:8399 bionet.microbiology:6706 bionet.molbio.methds-reagnts:47376 Hi! I am going to be growing up some 15N/13C labelled protein for an NMR analysis. I'm going to grow the bugs in M9 media supplemented with labelled NH4Cl and a labelled carbon source. From what I've read, labelled glucose is used, but it is very expensive. I was thinking of trying labelled acetate instead (I assume that it's much cheaper than glucose). I talked about this with one of my colleagues, and he said that there might be a problem with most of the label ending up in carbon dioxide. I was wondering if anybody has any thoughts on whether this would work; if I lose a lot of label as CO2, is that offset by the (presumed) savings in cost? By the way, I'm using E. coli strain BL21(DE3). Any tips on growing conditions, etc. would be welcome. Thanks! Matthew Parker From owner-structural-nmr@net.bio.net Sat Jul 27 23:00:00 1996 Path: biosci!agate!howland.reston.ans.net!vixen.cso.uiuc.edu!prairienet.org!glturner From: glturner@prairienet.org (Gary L. Turner) Newsgroups: bionet.structural-nmr Subject: Re: News Release Date: 28 Jul 1996 14:19:26 GMT Organization: University of Illinois at Urbana Lines: 20 Message-ID: <4tfste$g9r@vixen.cso.uiuc.edu> NNTP-Posting-Host: firefly.prairienet.org >> The objective of the M R Resources sales group is to listen to and understand >> the needs of its customers, and recommend the appropriate products and >> services which will address those needs in a cost effective manner. Lawrence, >> who is an experienced professional in NMR sales, brings to M R Resources a >> keen ability to develop long term relationships with his customers. >> Relationship building is a critical piece of M R Resources strategy. It >> allows its salespeople to quickly and thoroughly understand their customers >> situations and immediately respond with the proper solution. >> > > >BS Larry, Does BS = Brilliantly Spoken? Gary -- From owner-structural-nmr@net.bio.net Sun Jul 28 23:00:00 1996 Path: biosci!agate!news.ucdavis.edu!info.ucla.edu!newsfeed.internetmci.com!torn!resunix.ri.sickkids.on.ca!news From: Randall Willis Newsgroups: bionet.structural-nmr,bionet.molbio.proteins,bionet.microbiology,bionet.molbio.methds-reagnts Subject: Re: 13C source for protein NMR Date: 29 Jul 1996 13:26:20 GMT Organization: The Hospital for Sick Children Lines: 40 Message-ID: <4tie5s$43a@resunix.ri.sickkids.on.ca> References: <4tgau4$2kru@mule0.mindspring.com> NNTP-Posting-Host: resunix.ri.sickkids.on.ca Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) To: kindgus@bham.mindspring.com X-URL: news:4tgau4$2kru@mule0.mindspring.com Xref: biosci bionet.structural-nmr:1408 bionet.molbio.proteins:8402 bionet.microbiology:6711 bionet.molbio.methds-reagnts:47405 Matthew, Our lab routinely performs 15N/13C growths for NMR samples and we have worked with 13C-acetate in the past. While the acetate is about half the price of glucose, we found that our final protein yields were also reduced by ~50%, so it was something of an even trade off. Depending upon how well your protein expresses in minimal media, this may be okay for you as you may be able to get good samples from the lower yield or you may not see a difference with the expression in acetate. Typically, our yields were reduced for two reasons; the bacteria don't express as well on acetate and the overall OD's tend to be lower. I would recommend that you perform a number of growths with both carbon sources and take it from there. When we perform a growth, I usually perform a fresh transformation of my plasmid into the BL21(DE3) and then grow a colony in LB for a few hours to overnight. This is then used as an inoculum for the M9 growth which I induce anywhere from OD600=0.4 to 1.2 depending upon the construct and the culture. If you go with the acetate, you may want to perform an intermediate growth of M9-glucose before going into M9-acetate as we have found that the bugs behave better if you give them only one challenge per step (ie rich media to minimal...good carbon to poorer). If you need any more advice, drop a line. Good luck. Randall C Willis, Researcher Publisher (lab of Julie Forman-Kay) Aliquotes Press Biochemistry Research "ALIQUOTES:A Journal of Molecular & Hospital for Sick Children Biochemical Humour" 3522-555 University Ave. 58 Balfour Ave. Toronto, ON Toronto, ON M5G 1X8 CANADA M4C 1T6 CANADA 416-813-5933 (ph) 416-691-2921 (ph) 416-813-5022 (fax) willis@gandalf.psf.sickkids.on.ca rogerb@microsoft.com From owner-structural-nmr@net.bio.net Sun Jul 28 23:00:00 1996 Path: biosci!ncbi.ncbi.nlm.nih.gov!francis From: francis@ncbi.ncbi.nlm.nih.gov Newsgroups: bionet.structural-nmr Subject: Entrez version 5.0 Date: 29 Jul 1996 12:55:06 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 32 Sender: daemon@net.bio.net Distribution: world Message-ID: <199607291955.PAA21449@borduas.nlm.nih.gov> NNTP-Posting-Host: net.bio.net NCBI Announces Entrez 5.0 with Cn3D 3D Structure Viewer ======================================================= Entrez now provides a means to visualize molecular structure data. The viewer, Cn3D, is part of Network Entrez client programs and can also be used as a helper application for the Web version of Entrez. A full description of the Cn3D program may be found at the NCBI's Structure Group home page: http://www.ncbi.nlm.nih.gov/Structure Entrez provides an integrated software environment for retrieving and browsing nucleotide and protein sequences, the molecular genetics subset of MEDLINE, and NEW genomes and structures divisions. For more information about Entrez see: http://www3.ncbi.nlm.nih.gov/Entrez or send e-mail to: info@ncbi.nlm.nih.gov --------------------------------------------------------- National Center for Biotechnology Information National Library of Medicine, National Institutes of Health Bethesda, Maryland From owner-structural-nmr@net.bio.net Sun Jul 28 23:00:00 1996 Path: biosci!agate!newsxfer2.itd.umich.edu!newsfeed.internetmci.com!news.ac.net!news.cais.net!news2.digex.net!usenet From: numare@cnj.digex.net Newsgroups: bionet.structural-nmr Subject: Re: News Release Date: Mon, 29 Jul 96 09:28:02 PDT Organization: Express Access Online Communications, USA Lines: 21 Message-ID: References: <4tfste$g9r@vixen.cso.uiuc.edu> NNTP-Posting-Host: cjc08014.slip.digex.net Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII X-Newsreader: NEWTNews & Chameleon -- TCP/IP for MS Windows from NetManage > > > >BS > > Larry, > > Does BS = Brilliantly Spoken? > > Gary > -- > Gary, Not quite. Folks that are concerned with image rather than substance very often trip and are engulfed in their own muck. Lawrence From owner-structural-nmr@net.bio.net Mon Jul 29 23:00:00 1996 Path: biosci!ihnp4.ucsd.edu!scripps.edu!obelix!weber From: weber@obelix.scripps (Christoph Weber) Newsgroups: bionet.structural-nmr,bionet.molbio.proteins,bionet.microbiology,bionet.molbio.methds-reagnts Subject: Re: 13C source for protein NMR Date: 30 Jul 1996 20:42:00 GMT Organization: The Scripps Research Institute Lines: 23 Message-ID: <4tls2o$o4k@riscsm.scripps.edu> References: <4tgau4$2kru@mule0.mindspring.com> <4tie5s$43a@resunix.ri.sickkids.on.ca> NNTP-Posting-Host: obelix.scripps.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Keywords: NMR, 13C-glucose, 13C-acetate, protein Xref: biosci bionet.structural-nmr:1410 bionet.molbio.proteins:8413 bionet.microbiology:6721 bionet.molbio.methds-reagnts:47445 As an aside for those who think today's 13C-glucose prices are high: Back when we first started with the uniform labelling business, the glucose prices were way higher, by a factor of 4-5. I've been involved in a project where we set aside $40,000 just for 13C-glucose, and I have heard of projects that required substantially more money. Those were no low-yield expression systems either... Nevertheless, it still pays to improve the sample production process, no matter what the prices are. In general, I agree with the previous poster's observations about protein expression on acetate. There was a paper in Biochemistry in '90 or '91 with some good detail. The protein was carbonic anhydrase, if I'm not mistaken. Happy labelling, Christoph | Christoph Weber Sen. Research Associate | Dept.of Molecular Biology, MB2 619-554-7283 or -8754 (phone) | The Scripps Research Institute 619-554-3757 (FAX) | La Jolla CA 92037-1027 weber@scripps.edu | http://www.scripps.edu/~chazin/people/cw.html From owner-structural-nmr@net.bio.net Tue Jul 30 23:00:00 1996 Path: biosci!sandoz.com!Nanguneri.Nirmala From: Nanguneri.Nirmala@sandoz.com Newsgroups: bionet.structural-nmr Subject: Temporary Position in protein nmr spectroscopy Date: 31 Jul 1996 10:38:14 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 39 Sender: daemon@net.bio.net Distribution: world Message-ID: <0034700002125030000002*@MHS> NNTP-Posting-Host: net.bio.net We are looking to fill a TEMPORARY position in the Structural Biology Group at Sandoz Pharmaceuticals Corporation. This position involves the following: Implementation of automated assignment strategies for assignment of the NMR spectra of proteins. The position involves recording multidimensional NMR spectra and implementing automated assignment strategies to make assignments. The skills required would be the following: M.S. or Ph. D in a related field, familiarity with NMR spectroscopy of proteins, knowledge of an advanced programming language. This position is of a temporary nature - this means the job could exist this week and be gone the next. This position is NOT a post-doctoral fellowship. For consideration, please mail or fax resume to: Kelly Scientific Resources, 242 Old New Brunswick Road, Piscataway, NJ 08854. Attn. Lynn Titus Fax: 908-981-1377. Telephone enquiries are not encouraged. You may respond by e-mail if you wish but any interviews or further consideration will be arranged through Kelly Scientific Resources. N. R. Nirmala, Structural Biology Group, Sandoz Research Institute, Sandoz Pharmaceuticals Corporation, 59 Route 10, E. Hanover, NJ 07936. From owner-structural-nmr@net.bio.net Tue Jul 30 23:00:00 1996 Path: biosci!agate!howland.reston.ans.net!nntp.coast.net!news.sgi.com!news.msfc.nasa.gov!newsfeed.internetmci.com!info.ucla.edu!news.bc.net!torn!resunix.ri.sickkids.on.ca!news From: Randall Willis Newsgroups: bionet.structural-nmr,bionet.molbio.proteins,bionet.microbiology,bionet.molbio.methds-reagnts Subject: Re: 13C source for protein NMR Date: 31 Jul 1996 14:18:26 GMT Organization: The Hospital for Sick Children Lines: 21 Message-ID: <4tnpvi$f14@resunix.ri.sickkids.on.ca> References: <4tgau4$2kru@mule0.mindspring.com> <4tie5s$43a@resunix.ri.sickkids.on.ca> <4tls2o$o4k@riscsm.scripps.edu> NNTP-Posting-Host: resunix.ri.sickkids.on.ca Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) X-URL: news:4tls2o$o4k@riscsm.scripps.edu Xref: biosci bionet.structural-nmr:1413 bionet.molbio.proteins:8421 bionet.microbiology:6731 bionet.molbio.methds-reagnts:47472 A couple of references concerning the use of 13C-acetate are: Venters et al (1991) "Uniform 13C Isotope Labeling of Proteins with Sodium Acetate for NMR Studies: Application to Human Carbonic Anhydrase II" Biochemistry 30, 4491-4494. and, Venters et al (1995) "High-level 2H/13C/15N labeling of proteins for NMR studies" Journal of Biomolecular NMR 5, 339-344. Hope that these help. Randall C Willis, Researcher Biochemistry Research 416-813-5933 (ph) Hosp for Sick Children 416-813-5022 (fax) 3522-555 University Ave. Toronto, ON willis@gandalf.psf.sickkids.on.ca M5G 1X8 CANADA From owner-structural-nmr@net.bio.net Tue Jul 30 23:00:00 1996 Path: biosci!LAPLACE.CSB.YALE.EDU!gwarren From: gwarren@LAPLACE.CSB.YALE.EDU (Greg Warren) Newsgroups: bionet.structural-nmr Subject: proline parameters Date: 31 Jul 1996 06:19:29 -0700 Organization: Yale University Lines: 23 Sender: daemon@net.bio.net Distribution: world Message-ID: <31FF5D6B.2781@laplace.csb.yale.edu> NNTP-Posting-Host: net.bio.net As an addendum to John Kuszewski's response to the proline parameter problem, the current parallhdg.pro at the XPLOR ftp site contains the modifications John talked about. This file is accessible to the public at ftp://xplor.csb.yale.edu/pub/toppar/ -- =========================================================== Gregory L. Warren, PhD Department of Molecular Biophysics and Biochemistry Yale University 266 Whitney Avenue New Haven, CT 06520-8114 USA (203) 432-5066 (203) 432-3923 - fax gwarren@laplace.csb.yale.edu Home address: 91 Bishop St. Apt 11 New Haven, CT 06511 =========================================================== From owner-structural-nmr@net.bio.net Wed Jul 31 23:00:00 1996 Newsgroups: bionet.structural-nmr Path: biosci!agate!newsgate.duke.edu!news-server.ncren.net!hearst.acc.Virginia.EDU!murdoch!faraday.clas.Virginia.EDU!jj8a From: jj8a@faraday.clas.Virginia.EDU ([31mThe Brewer [37m) Subject: Unsymmetrical amide NMR question X-Nntp-Posting-Host: faraday.clas.virginia.edu Message-ID: Sender: usenet@murdoch.acc.Virginia.EDU Organization: University of Virginia X-Newsreader: TIN [version 1.2 PL1] Date: Thu, 1 Aug 1996 03:25:18 GMT Lines: 7 I am desparately in need of a literature reference which states that N-methyl amides (-CONHCH3) show restricted rotation on the NMR timescale (You see two sets of peaks). I have references which describe disubstituted amides. Help... -- From owner-structural-nmr@net.bio.net Wed Jul 31 23:00:00 1996 Path: biosci!PHOENIX.PRINCETON.EDU!ipelczer From: ipelczer@PHOENIX.PRINCETON.EDU (Istvan Pelczer) Newsgroups: bionet.structural-nmr Subject: Re: Unsymmetrical amide NMR question Date: 1 Aug 1996 09:34:25 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 28 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net I recall a good book on DNMR from Sandstrom back in time (hope I remember well his name...); you might find something there. Good luck, Istvan wwww,wwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwww Istvan Pelczer, Ph.D. Email: ipelczer@princeton.edu Senior NMR Spectroscopist Princeton University Department of Chemistry ph# (609) 258 2342 Frick Laboratory fax# (609) 258 6746 Washington Road Princeton, NJ 08544 On Thu, 1 Aug 1996 jj8a@faraday.clas.Virginia.EDU wrote: > I am desparately in need of a literature reference > which states that N-methyl amides (-CONHCH3) show restricted rotation on > the NMR timescale (You see two sets of peaks). I have > references which describe disubstituted amides. > Help... > -- > > > From owner-structural-nmr@net.bio.net Wed Jul 31 23:00:00 1996 Path: biosci!BIOC01.UTHSCSA.EDU!raman From: raman@BIOC01.UTHSCSA.EDU (C.S.RAMAN) Newsgroups: bionet.structural-nmr Subject: Faculty Position in Structural NMR - ANNOUNCEMENT Date: 1 Aug 1996 12:27:44 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 53 Sender: daemon@net.bio.net Distribution: world Message-ID: <9608011926.AA03448@bioc01.uthscsa.edu> NNTP-Posting-Host: net.bio.net Dear STR-NMR readers: The DEPARTMENT OF BIOCHEMISTRY at THE UNIVERSITY OF THEXAS HEALTH SCIENCE CENTER AT SAN ANTONIO invites applications for a twelve-month, state-funded, tenure-track faculty position at the assistant or associate professor level. Candidates with research expertise in any area of contemporary biocchemistry will be considered. Special consideration will be given to candidates with research interest in the following area: MACROMOLECULAR STRUCTURE - high resolution structure (X-ray crystallography, NMR) and the dynamics of macromolecules. Interested applicants should send a curriculum vitae, a brief research prospectus and the names of thre references to: Faculty Search Committee Department of Biochemistry The University of Texas Health Science Center at San Antonio 7703 Floyd Curl Drive San Antonio, TX 78284-7760 For information about the Department of Biochemistry, see: http://bioc02.uthscsa.edu/biochem.html The deadline for applicationss is OCTOBER 1, 1996. An Affirmative Action/Equal Opportunity Employer. Cheers -raman -- _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/ _/ _/ _/ C.S.RAMAN _/ _/ Department of Biochemistry _/ _/ University of Texas Health Science Center _/ _/ 7703 Floyd Curl Drive _/ _/ San Antonio, TX 78284-7760 _/ _/ USA _/ _/ _/ _/ Tel: (210) 614-0839 _/ _/ Fax: (210) 567-6595 _/ _/ E-mail: raman@bioc01.uthscsa.edu _/ _/ _/ _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/ _/ _/ _/ "The real problem in speech is not precise language. _/ _/ The problem is clear language." --Richard Feynman _/ _/ _/ _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/ From owner-structural-nmr@net.bio.net Wed Jul 31 23:00:00 1996 Path: biosci!CARIBE.CHEM.UKY.EDU!cammers From: cammers@CARIBE.CHEM.UKY.EDU (Arthur Cammers) Newsgroups: bionet.structural-nmr Subject: Re: Unsymmetrical amide NMR question Date: 1 Aug 1996 06:21:43 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 23 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net On Thu, 1 Aug 1996, [31mThe Brewer [37m wrote: > I am desparately in need of a literature reference > which states that N-methyl amides (-CONHCH3) show restricted rotation on > the NMR timescale (You see two sets of peaks). I have > references which describe disubstituted amides. You probably won't find these because the trans form of N-methylamides dominates the equilibrium. The amount of cis N-methylamide will always be below the nmr threshold. O || N---C | | Me R cis Me O | || N---C | R trans Arthur Cammers-Goodwin