From owner-structural-nmr@net.bio.net Fri Nov 01 22:00:00 1996 Path: biosci!INDIANA.EDU!mastone From: mastone@INDIANA.EDU (Martin J. Stone) Newsgroups: bionet.structural-nmr Subject: NMR Postdoctoral Position Available Date: 2 Nov 1996 10:29:13 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 73 Sender: daemon@net.bio.net Distribution: world Message-ID: <199611021829.NAA21168@cayman.ucs.indiana.edu> NNTP-Posting-Host: net.bio.net I would appreciate people posting/distributing this notice and bringing it to the attention of potential applicants. Thanks. ********************************************************************** NMR POSTDOCTORAL POSITION - PROTEIN STRUCTURE/FUNCTION - AVAILABLE NOW ********************************************************************** WHERE? Martin Stone's lab. Department of Chemistry (Program in Biochemistry), Indiana University, Bloomington, Indiana. WHEN? The position is available immediately! RESEARCH TOPIC? The position will involve investigation of the structure-function relationships of chemokines (chemotactic cytokines). Chemokines are small soluble proteins involved in the recruitment of leukocytes in many inflammatory diseases. Inhibition of chemokine activity may be an effective strategy against these diseases. Recently, it has also been discovered that chemokines are exteremely important in controlling the infection of T-cells by HIV. Projects in the lab involve 3D structure and dynamics characterization of chemokines by NMR and also a range of mutagenesis and functional studies. The emphasis is on using structural information to develop novel inhibition strategies. EXPERIENCE REQUIRED Applicants should have experience in multidimensional NMR techniques. Experience in molecular biology (subcloning, mutagenesis) and protein expression and purification would be a major advantage. Experience in any of the following areas is a distinct advantage. Triple-resonance and 3D NMR Pulsed field gradient NMR Structure calculations and modeling FACILITIES AVAILABLE A fully equipped biochemistry/molecular biology lab. A 500 MHz Varian UnityINOVA NMR spectrometer equipped with 3 RF channels, 28 shims, and pulsed field gradients (3-axis gradients are on order). The Stone group gets 50% time on this instrument. An extensive network of Silicon Graphics workstations with Biosym/Molecular Simulations software. FURTHER DETAILS (1) See our world wide web page: http://pooh.chem.indiana.edu/ (2) Contact Martin Stone email: mastone@indiana.edu Tel: (812)855-6779 TO APPLY Please submit CV, cover letter, and names and addresses (including telephone, fax, email) of at least two referees to: Prof. Martin J. Stone, Department of Chemistry, Indiana University, Bloomington, IN 47405-4001. Tel: (812) 855-6779 fax: (812) 855-8300 email: mastone@indiana.edu ======================================= Martin J. Stone, Assistant Professor of Biochemistry, Department of Chemistry, Indiana University, Bloomington, IN 47405. Tel. (812)855-6779 Fax. (812)855-8300 email. mastone@indiana.edu www: http://pooh.chem.indiana.edu/ From owner-structural-nmr@net.bio.net Fri Nov 01 22:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.erols.net!news3.cac.psu.edu!news.cse.psu.edu!news.cc.swarthmore.edu!netnews.upenn.edu![130.91.171.101]!chris From: chris@highresnmr.biophys.upenn.edu (Krzysztof Wroblewski) Newsgroups: bionet.structural-nmr Subject: magnet shielding Date: Fri, 1 Nov 1996 19:16:39 Organization: University of Pennsylvania Lines: 7 Message-ID: NNTP-Posting-Host: nmprc2.biophys.upenn.edu X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] My lab is going to be moved to the new place where all my magnets (300MHz, 360MHz, 500MHz 600MHz) will need to be shielded. There are offices over this place and a big machine shop underneath. Does anybody have any experience with shielding of high field magnets and running the NMR lab in such nice environment? Krzysztof Wroblewski From owner-structural-nmr@net.bio.net Fri Nov 01 22:00:00 1996 Path: biosci!daresbury!not-for-mail From: "Thomas Haselhorst" Newsgroups: bionet.structural-nmr Subject: QUIET-NOESY ??? Date: 2 Nov 1996 12:57:25 -0000 Lines: 22 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <55fgfl$9jn@mserv1.dl.ac.uk> Original-To: str-nmr@dl.ac.uk Dear netters !! We have some problems with spindiffusion in our NOESY experiments and we want to perform a QUIET-NOESY experiment. Has anybody experience with QUIET-NOESY. Would it be possible that someone sends us the pulse sequence ?? We have a Bruker AVANCE DRX spectrometer. Thanks in advance ! Best regards ! ************************************************************************ Thomas Haselhorst e-mail : thomas@johann.chemie.mu-luebeck.de Institute of Chemistry Medical University of Luebeck Tel. x49 451 500 4239 Ratzeburger Allee 160 Fax. x49 451 500 4241 23538 Luebeck, Germany ************************************************************************ From owner-structural-nmr@net.bio.net Sun Nov 03 22:00:00 1996 Path: biosci!PHOENIX.PRINCETON.EDU!ipelczer From: ipelczer@PHOENIX.PRINCETON.EDU (Istvan Pelczer) Newsgroups: bionet.structural-nmr Subject: Re: QUIET-NOESY ??? Date: 4 Nov 1996 14:12:24 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 45 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <55fgfl$9jn@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net Dear Thomas, I would certainly contact Slobodan Macura, who published several pioneering papers on this issue (macura@tesla.mayo.edu) -- and has Bruker. Istvan wwww,wwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwww Istvan Pelczer, Ph.D. Email: ipelczer@princeton.edu Senior NMR Spectroscopist Princeton University Department of Chemistry, Frick Lab., ph# (609) 258 2342 Washington Road fax# (609) 258 6746 Princeton, NJ 08544, USA On 2 Nov 1996, Thomas Haselhorst wrote: > Dear netters !! > > We have some problems with spindiffusion in our NOESY experiments > and we want to perform a QUIET-NOESY experiment. Has anybody experience with > QUIET-NOESY. Would it be possible that someone sends us the pulse sequence ?? > We have a Bruker AVANCE DRX spectrometer. > > Thanks in advance ! > > Best regards ! > > > ************************************************************************ > Thomas Haselhorst e-mail : thomas@johann.chemie.mu-luebeck.de > Institute of Chemistry > Medical University of Luebeck Tel. x49 451 500 4239 > Ratzeburger Allee 160 Fax. x49 451 500 4241 > 23538 Luebeck, Germany > ************************************************************************ > > > > > From owner-structural-nmr@net.bio.net Sun Nov 03 22:00:00 1996 Path: biosci!PHOENIX.PRINCETON.EDU!ipelczer From: ipelczer@PHOENIX.PRINCETON.EDU (Istvan Pelczer) Newsgroups: bionet.structural-nmr Subject: Re: shigemi tubes Date: 4 Nov 1996 13:57:59 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 45 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <3279B3D1.167E@roche.com> NNTP-Posting-Host: net.bio.net Dear Alfred, I learned recently that Shigemi performs all operations for Europe from the US office, too. You can contact them directly at the Email address; 740-7376@mcimail.com. All the best, Istvan wwww,wwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwww Istvan Pelczer, Ph.D. Email: ipelczer@princeton.edu Senior NMR Spectroscopist Princeton University Department of Chemistry, Frick Lab., ph# (609) 258 2342 Washington Road fax# (609) 258 6746 Princeton, NJ 08544, USA On 1 Nov 1996, Alfred Ross wrote: > Dear netters, > > does anybody know the adress of a branch/agency of the shigemi company > located in europe where it might be possible to get a catalogue etc. > > thanks for any hint > > -- > ************************************* > Dr. Alfred Ross > NMR-Spectroscopist > > e-mail: alfred.ross@roche.com ******* > Phone: CH-(0)61-6887029 * * > Fax: CH-(0)61-6887408 * ROCHE * > * * > Mail: F. Hoffmann-LaRoche AG ******* > (A. Ross - PRPS) > Postfach > CH-4070 Basel > ************************************* > > From owner-structural-nmr@net.bio.net Sun Nov 03 22:00:00 1996 Path: biosci!NMRSGI2.NCIFCRF.GOV!rabyrd From: rabyrd@NMRSGI2.NCIFCRF.GOV (R. Andrew Byrd) Newsgroups: bionet.structural-nmr Subject: Magnet Shielding Date: 4 Nov 1996 09:39:20 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 33 Sender: daemon@net.bio.net Distribution: world Message-ID: <199611041731.MAA02672@nmrsgi2.ncifcrf.gov> NNTP-Posting-Host: net.bio.net In response to the recent request of Krzysztof Wroblewski and the response from Bill Hutton, I will just add some simple comments: We have successfully shielded the space above an Oxford 600 magnet without either deleterious effects to the magnet homogeneity or the space and personnel above. True, if there are sensitive areas in all directions from the lab, I concur with Bill, and it may be more advantageous to find another location. However, if one only has to worry about the space in one vertical direction from the magnet(s), then I feel it is reasonable, and not so expensive to shield the space. I do not claim any particular expertise in this area, but I would [and have] refer you to Rolf Tschudin at the NIH. He was the one who introduced this procedure to me and offered some valuable advice. They have experience in shielding a number of magnets there...but again in only one direction. Of course, each site is unique, and the concerns of cost and weight indicated by Bill will clearly reflect his own site(s). One concern of the manufacturers [e.g. Oxford and others] is to offer guarantees...since each site is unique. Nevertheless, I believe that many sites can be made operational with some forethought. Best regards, Andy ------------------ Dr. R. Andrew Byrd Macromolecular NMR/MSL/ABL TEL: 301-846-1407 FAX: 301-846-6195 From owner-structural-nmr@net.bio.net Sun Nov 03 22:00:00 1996 Path: biosci!daresbury!hgmp.mrc.ac.uk!news From: Mike Gradwell Newsgroups: bionet.structural-nmr Subject: Re: shigemi tubes Date: Mon, 04 Nov 1996 08:55:04 +0100 Organization: National Institute for Medical Research Lines: 18 Message-ID: <327DA158.2781E494@nimr.mrc.ac.uk> References: <3279B3D1.167E@roche.com> NNTP-Posting-Host: nmrsn44.nimr.mrc.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01b1 (X11; I; SunOS 4.1.3 sun4m) Campro Scientific are a company based in the Netherlands who distribute Shigemi tubes. They can be contacted as follows: Telephone: (+31)318 52 94 37 Fax: (+31)318 54 21 81 email: campro@pi.net The main contact for enquiries is Willem Priem, for best results fax him describing the info you'll need. I also have some prices if you're interested, Alfred. Email me if necessary. Good Luck -- Mike Gradwell, MRC Biomedical NMR Centre, National Institute for Medical Research, Mill Hill, London NW7 1AA. Tel. +44181 959 3666 ext. 2026 Fax. +44181 906 4477 From owner-structural-nmr@net.bio.net Sun Nov 03 22:00:00 1996 Path: biosci!rutgers!gatech!csulb.edu!newshub.csu.net!www.nntp.primenet.com!nntp.primenet.com!feed1.news.erols.com!howland.erols.net!newsfeed.internetmci.com!newsfeeder.gi.net!tin.monsanto.com!usenet From: Bill Newsgroups: bionet.structural-nmr Subject: Re: magnet shielding Date: Mon, 04 Nov 1996 10:26:14 +0000 Organization: Monsanto Company Lines: 16 Message-ID: <327DC4C6.1994@mindspring.com> References: Reply-To: bill_911@mindspring.com NNTP-Posting-Host: wchutt.monsanto.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.02 (Macintosh; I; 68K) Krzysztof Wroblewski wrote: > > My lab is going to be moved to the new place where all my magnets > (300MHz, 360MHz, 500MHz 600MHz) will need to be shielded. There are offices > over this place and a big machine shop underneath. Does anybody have any > experience with shielding of high field magnets and running the NMR lab > in such nice environment? We were told the mass of steel required to give proper shielding meant attaching the steel to the ceiling/walls/floor was extremely expensive (>$100,000). I suggest you contact Oxford Instruments or an archetectural firm that builds MRI facilities for hospitals. Bill Hutton Monsanto Company From owner-structural-nmr@net.bio.net Mon Nov 04 22:00:00 1996 Path: biosci!daresbury!not-for-mail From: "James Molecule" Newsgroups: bionet.structural-nmr Subject: structure of Arginine side chain in DMSO Date: 5 Nov 1996 09:55:51 -0000 Lines: 3 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <55n2v7$i11@mserv1.dl.ac.uk> Reply-To: maliekal@psipsy.uct.ac.za X-mailer: Pegasus Mail v3.22 Original-To: str-nmr@dl.ac.uk Does anyone know the structure of Arginine side chain in DMSO solution? Is it protonated or not? From owner-structural-nmr@net.bio.net Mon Nov 04 22:00:00 1996 Path: biosci!aol.com!Martek2000 From: Martek2000@aol.com Newsgroups: bionet.structural-nmr Subject: Re. 13C-Glucose Date: 5 Nov 1996 14:14:43 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 3 Sender: daemon@net.bio.net Distribution: world Message-ID: <961105171406_222876822@emout17.mail.aol.com> NNTP-Posting-Host: net.bio.net Martek Biosciences Corp. has cut the cost of its 13C-Glucose by 25%. For information call 800-338-9959 or 410-740-0081. From owner-structural-nmr@net.bio.net Tue Nov 05 22:00:00 1996 Path: biosci!OLIGO.UTMB.EDU!quyx From: quyx@OLIGO.UTMB.EDU (You Xing Qu) Newsgroups: bionet.structural-nmr Subject: saturation transfer Date: 6 Nov 1996 09:25:37 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 38 Sender: daemon@net.bio.net Distribution: world Message-ID: <199611061727.LAA03730@oligo.utmb.edu> NNTP-Posting-Host: net.bio.net Dear netters, I have some questions about saturation transfer. Perhaps they are simple ones, but the answers to them are important to my work and to a beginner like me. Your kind help is very much appreciated. I am working on the hydrogen exchange rate measurements of Poly-DL-Alanine (PDLA) amide protons in 90%H2O/10%D2O at 35 degrees and different pHs ranging from pH 3 to 7, using the saturation transfer method. ( For references on saturation transfer,please find: Dempsey,1986,Biochemistry.25:3904-3911; O'Neil & Sykes,1989.Biochemistry.28,699-707.) People normally use continuous preirridiation to saturate water. We tried using Pulsed Field Gradients (PFG) for water saturation, but we found the obtained hydrogen exchange rates by PFG were systematically smaller than those obtained by continuous preirradiation (Wang et al., 1995,Biochemistry.34:15096-15104) and/or the predicted PDLA NH exchange rates calculated using Bai et al. & Englander's data ( Bai et al., 1993. Proteins.17:75-86; Connelly et al.,1993.Proteins.17:87-92.) . So, here are my questions: 1. Theoretically and experimentally, can PFG be used for saturation transfer measurements? 2. Both in continuous preirradiation and PFG (if it can be used for saturation transfer), how to know water is really SATURATED rather than merely suppressed? Are there any objective criteria to follow? For the preirradiation time, the longer the better to saturate water? In PFG we cannot use such a long irradiation time as in continuous preirridiation. Sincerely, Youxing Youxing Qu Dept. of Human Biological Chemistry and Genetics Univ. of Texas Medical Branch at Galveston Galveston, TX 77555-1052, USA E-mail: quyx@oligo.utmb.edu From owner-structural-nmr@net.bio.net Thu Nov 07 22:00:00 1996 Path: biosci!daresbury!nntp-trd.UNINETT.no!nntp.uio.no!www.nntp.primenet.com!nntp.primenet.com!howland.erols.net!math.ohio-state.edu!jussieu.fr!oleane!plug.news.pipex.net!pipex!hole.news.pipex.net!pipex!bowl.news.pipex.net!pipex!news00.sunet.se!sunic!news99.sunet.se!umdac!news From: "Pär Wästerby" Newsgroups: bionet.cellbiol,bionet.general,bionet.info-theory,bionet.metabolic-reg,bionet.molbio.proteins,bionet.molecules.peptides,bionet.structural-nmr,sci.med.pharmacy Subject: A novel Progesterone Researsh Project Date: 8 Nov 1996 13:40:17 GMT Organization: Fysikalisk kemi Lines: 7 Message-ID: <01bbcd58$e8fc8520$3024ef82@pepsi> NNTP-Posting-Host: pepsi.chem.umu.se X-Newsreader: Microsoft Internet News 4.70.1155 Xref: biosci bionet.cellbiol:5894 bionet.general:23924 bionet.info-theory:4368 bionet.metabolic-reg:871 bionet.molbio.proteins:9221 bionet.molecules.peptides:501 bionet.structural-nmr:1598 sci.med.pharmacy:36113 Dear all, we would really apprecieate if there is someone that could give us information about Progesterone and its metabolites. Since we are going to do chromotographic separation, we are interested in physical data, structure data and acid - base properties (pKa values). It would also be very helpful to find a review aritcle in the area. From owner-structural-nmr@net.bio.net Wed Nov 13 22:00:00 1996 Path: biosci!rutgers!uwm.edu!cs.utexas.edu!news.tamu.edu!news.utdallas.edu!nrchh45.rich.nt.com!bcarh189.bnr.ca!nott!nrcnet0.nrc.ca!BRI.NRC.CA!Feng.Ni From: Feng.Ni@BRI.NRC.CA (Feng Ni) Newsgroups: bionet.structural-nmr Subject: RESEARCH POSITIONS IN PROTEIN STRUCTURE AND DESIGN Date: 14 Nov 1996 21:28:45 GMT Organization: Institut de recherche en biotechnologie, Montréal Lines: 46 Distribution: world Message-ID: <56g2ud$id6@nrcnet0.nrc.ca> NNTP-Posting-Host: indy.bri.nrc.ca RESEARCH POSITIONS IN PROTEIN STRUCTURE AND DESIGN The Biotechnology Research Institute (BRI) has openings for self-motivated researchers to study the structure-and-function relationships of proteins and to design novel proteins or peptides as potential pharmaceutical agents. The research will be focusing on the following areas: (1) proprotein convertases (PC's), calcium-sensing receptors (CaSRs), and CDC42-target complexes. Candi- dates should have a recent Ph.D. (within the last 5 years) in chemistry, bio- chemistry or a related field and have demonstrated abilities in carrying out creative research. Experiences include protein expression, purification and characterization, and the analysis of the structures of proteins by use of NMR or other biophysical techniques. Preferences will be given to candidates with extensive knowledge in the conformational stabilities and folding of proteins and peptides. Experience in the determination of the 3D structures of proteins by NMR is an important asset. Successful applicants will start in the postdoctoral level, or depending on qualifications, can be recruited to more senior positions offerred by the National Research Council of Canada. The Research Council offers competitive salaries and other compensation packages commensurate upon your experience and appointment levels. Researchers have access to state-of-the-art facilities for protein biochemistry. Structural analysis is carried out with two (500 and 800 MHz) NMR spectrometers along with required computing facilities. BRI offers an intellectually stimulating environment for multi-disciplinary research with expertise in molecular biology, protein/peptide chemistry, and structural biology. The Biotechnology Research Institute is a branch of the National Research Council of Canada, interfacing academia and industry. Your challenge will be to succeed in high-impact scientific research while generating applications transferable to pharmaceutical industry. Qualified individuals are invited to submit your resume, along with the names and addresses of three references, to: Feng Ni, Ph.D. Biotechnology Research Institute 6100 Royalmount Avenue Montreal, Quebec, H4P 2R2, Canada phone: (514)-496-6729; fax: (514)-496-5143 e_mail: fengni@bri.nrc.ca The positions will be available after January 1, 1997. Applications will be accepted until the positions are filled. We thank all applicants IN ADVANCE for their interests, however, only those being considered will be contacted. From owner-structural-nmr@net.bio.net Sun Nov 17 22:00:00 1996 Path: biosci!PICASSO.UCSF.EDU!schmitz From: schmitz@PICASSO.UCSF.EDU (Ulrich Schmitz) Newsgroups: bionet.structural-nmr Subject: Postdoc in RNA structure determination Date: 18 Nov 1996 11:38:26 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 41 Sender: daemon@net.bio.net Distribution: world Message-ID: <199611181938.LAA11315@picasso.ucsf.EDU> NNTP-Posting-Host: net.bio.net Postdoctoral Position in Structure Determination of RNA with NMR University of California San Francisco in the lab of Uli Schmitz and Thomas L. James With a starting date as early as January 1997, a post-doctoral position is available in the lab of Tom James and Uli Schmitz in the Department of Pharmaceutical Chemistry at the University of California San Francisco We are currently engaged in the structure determination of double-labeled RNA fragments using multidimensional heteronuclear NMR methods. The NMR facility at UCSF is equipped with two GE 500MHz and one Varian UnityPlus 600MHz spectrometer (PFG equipment). A network of Sparc workstations, SGI Indigos and Macintosh computers is available for data processing and analysis.For structure refinement work we have access to a powerful HP cluster and several supercomputers. Besides a number of UCSF software packages (i.e., AMBER, MARDIGRAS, SPARKY, MIDADPLUS) we also have several commercial modeling and NMR processing software packages available. UCSF offers a rather unique, open and stimulating research environment with breathtaking views. Potential candidates should have a solid background in NMR and possibly some experience with heteronuclear NMR. Interested candidates should contact Uli Schmitz by mail or email. More information about the NMR facility and the ongoing work is available on the world wide web URL: http://picasso.ucsf.edu/~james ><><><><><><><><><><><><><><><><><><> Uli Schmitz Asst. Adjunct Professor UC San Francisco Dept. Pharmaceutical Chemistry San Francisco, CA 94143-0446 Phone (415) 476 8780; Fax (415) 476 0688; home (415) 380 8345; schmitz@picasso.ucsf.edu URL http://picasso.ucsf.edu/~schmitz ><><><><><><><><><><><><><><><><><><> From owner-structural-nmr@net.bio.net Wed Nov 20 22:00:00 1996 Path: biosci!ihnp4.ucsd.edu!gondor!sol.ctr.columbia.edu!spool.mu.edu!uwm.edu!cs.utexas.edu!howland.erols.net!news2.digex.net!news From: numare@cnj.digex.net Newsgroups: bionet.structural-nmr Subject: Re: Fa$t Ca$h Date: Wed, 20 Nov 96 19:23:44 PDT Organization: DIGEX Lines: 236 Message-ID: References: <328e5156.239727079@news.aa.net> NNTP-Posting-Host: dyn000218.plfd.digex.net Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII X-Newsreader: NEWTNews & Chameleon -- TCP/IP for MS Windows from NetManage In article <328e5156.239727079@news.aa.net>, writes: > Path: news2.digex.net!howland.erols.net!newsfeed.internetmci.com!news.us.world.net!ne ws.e-z.net!ixa.net!news.aa.net!usenet > From: theman@theplace.com (Dewey, Cheatem, and Howe) > Newsgroups: bionet.structural-nmr > Subject: Fa$t Ca$h > Date: Sat, 16 Nov 1996 23:44:28 GMT > Organization: Law Firm > Lines: 215 DIE YOU PIG > THIS IS FOR PEOPLE WHO ARE REALLY INTERESTED IN GETTING FASTCASH. IF > YOU ARE > READING THIS TO REPORT ANY OF THE PEOPLE IN THE LIST, YOU ARE LAME, > YOU SUCK, AND YOU HAVE NO LIFE. AND YOU NEED TO MIND YOUR OWN > BUSINESS. THIS DOESN'T > CONCERN YOU PEOPLE, SO FUCK OFF, AND GO AWAY. READ BELOW... > _____________________________________________________________________________ > > Believe it or not, you will get fast easy cash by just sending $5, > yes, just 5 dollars. You will receive $25,000 to $50,500 within 3 to 4 > weeks. Please take a couple minutes to read the rest. You will see > how it works. Act today, and just wait for your money to arrive at > your mailbox. Hello! I've got some awesome news that I think you need > to take two minutes to read if you have ever thought "How could I make > some serious > cash in a hurry???", or been in serious debt, ready to do almost > anything to get the money needed to pay off those bill colectors. So > grab a snack, a warm cup of coffee, or a glass of your favorite > beverage, get > comfortable and listen to this interesting, excting find, that even a > 13 year old can do! Let me start by saying that I FINALLY FOUND IT! > That's right! I found it! And I HATE GET RICH QUICK SCHEMES!! I hate > those schemes like multi-level marketing, mail-order schemes, envelope > stuffing scams, 900 number scams... > the list goes on forever. I have tried every darnn get rich quick > scheme out there over the past 12 years. I somehow got on mailing > lists for people looking to make money (more like 'desperate stupid > people who will try anything for money!). Well, when I was a teenager, > these claims to 'get me rich quick' sounded irresistable! I would > shell out $14.95 here, $29.95 there, $24.95 here, and another $49.95 > there. I had maxed out my new Circuit City Card AND my Visa... I was > desperate for money!! So, I gave them all a chance but failed at every > one of them! Maybe they worked for some people, but not for me. > Eventually, I just tossed that JUNK MAIL in the trash when I got the > mail. I recognized it right away. I can smell a money scam from a mile > away these days, SO I THOUGHT... I thought I could sniff out a scam > easily. WAS I WRONG!!... I LOVE THE INTERNET!!! > > I was scanning through a NEWSGROUP and saw an article stating to GET > CASH FAST!! I thought... "Here on the internet??? Well I'll just have > to see what schemes could possibly be on the internet." The article > described a way to MAIL A ONE DOLLAR BILL TO ONLY FIVE PEOPLE AND MAKE > $50,000 IN CASH WITHIN 4 WEEKS! Well, the more I thought about it, the > more I became more curious. Why? Because of the way it worked AND > BECAUSE IT WOULD ONLY COST ME FIVE DOLLARS (AND FIVE STAMPS), THAT'S > ALL I EVER PAY... EVER!! > > Ok, so the $50,000 in cash was maybe a tough amount to reach, but it > was possible. I knew that I could at least get a return of $1,000 or > so. So I did it!! As per the instructions in the article, I mailed out > ('snail > mail' for you e-mail fanatics) a single dollar bill to each of the > five people on the list that was contained in the article. I included > a small note, with the dollar, that stated "Please Add Me To Your > List." I then removed the first position name of the five names listed > and moved everyone up one position, and I put my name in position five > of the list. This is how the money starts rolling in! I then took this > revised article now with my > name on the list and REPOSTED IT ON AS MANY NEWSGROUPS AND LOCAL > BULLETIN BOARD MESSAGE AREAS THAT I KNEW. I then waited to watch the > money come in... prepared to maybe receive about $1000 to $1500 in > cash or so... But what a welcome surprise when those envelopes kept > coming in!!! I knew what they were as soon as I saw the return > addresses from people all over the > world-Most from the U.S., but some from Canada, even some from > Australia! I tell you, THAT WAS EXCITING!! So how much did I get in > total return? $1000? $5000? Not even!!! I received a total of > $23,343!!! I couldn't believe it!! > > I now have a brand new black Acura Integra to speak for, due to this!! > Now after almost 8 months, I am ready to do it again!!! So maybe it > was possible to get $50,000 in cash, I don't know, but IT COMPLETELY > DEPENDS ON YOU, THE INDIVIDUAL! You must follow through and repost > this article everywhere you can think of! The more postings you > achieve will determine how much cash will arrive in your very own > mailbox!! It's just too easy to pass up!!! > > Let's review the reasons why you should do this: The only cost factors > are for the five stamps, the five envelopes, and the five one dollar > bills that you send out to the listed names by snail mail (US Postal > Service Mail). Then just simply repost the article (WITH YOUR NAME > ADDED) to all the newsgroups and local BBS's you can. Then sit back > and (ironically), enjoy walking (you can run if you like! :-o) down > your driveway to your mailbox and scoop up your rewards!! We all have > five dollars to put into such an easy effortless investment with > SPECTACULAR REALISTIC RETURNS OF $15,000 to $25,000 in about 3-5 > weeks! So HOLD OFF THOSE LOTTERY NUMBERS FOR TODAY, EAT AT HOME > TONIGHT INSTEAD OF TAKEOUT FROM McDONALD'S AND INVEST FIVE DOLLARS IN > THIS AMAZING MONEY SYSTEM NOW!!! YOU CAN'T LOSE!!! So how do you do > it exactly, you ask? I have carefully provided the most detailed, yet > straightforward instructions on how to easily get this underway and > get your cash on its way. SO, ARE YOU READY TO MAKE SOME CASH!?!?!? > HERE WE GO!!! > > *** THE LIST OF NAMES IS AT THE END OF THIS ARTICLE *** > > OK, Read this carefully. Get a prinout of this information, if you > like, so you can easily refer to it as often as needed. > > INSTRUCTIONS: > > 1. Take a sheet of paper and write on it the following: "Please add my > name and address to your list". This creates a service out of this > money making system and thus making it completely legal. You are not > randomly sending a dollar to someone, you are paying one dollar for a > legitimate service. Make sure you include your name, address, and > e-mail address. I assure you that, again, this is completely legal! > For a neat little twist, also write what slot their name was in : "You > were in slot 3", Just to add a little fun! This is all about having > fun and making money at the same time! > > 2. Now fold this sheet of paper around a dollar bill (no checks or > money orders) and put them into an envelope and send it on its way to > the five people listed. The folding of the paper around the bill will > insure its arrival to its recipient. THIS STEP IS IMPORTANT!! > > 3. Now listen carefully, here's where you GET YOUR MONEY COMING TO > YOUR MAILBOX. Look at the list off five people; remove the first name > from position one and move everyone on the list up slot one on the > list. Position 2 will now move to the position 1 slot, position 3 > will now become position 2, and so on. Now put your name, address, > zipcode, and country in position 5, the bottom position on the list. > > 4. Now upload this ubdated file to as many newsgroups and local > bulletin boards' message areas & file section as possible. (Preferably > about 200, if you have the time) The more you post it the more chances > you have someone responding to it. Give a catchy description of the > file so it gets noticed!! Such as: "NEED FAST CASH", HERE IT IS!" or > "$$$ Fa$t Ca$t $$$", etc. And the more uploads, the more money you > will make, and of course, the more money the others on the list will > make too. LET'S ALL TAKE CARE OF EACH OTHER BY BEING HONEST AND BY > OUTTING FORTH 120 PERCENT INTO THIS PROFITABLE & AMAZING SYSTEM!!! > You'll reap the benefits, believe me!!! Set a goal for the > number of totol uploads you'll post, such as 15-20 postings or more! > Always have a goal in mind!! If you can UUE encode the file when > uploading, that will make it easier for the people to receive it and > have it downloaded to their hard drive. That way they get a copy of > the article right on their computer without hassles of viewing and > then saving the article from the File menu. Don't alter the file type, > leave it as an MS-DOS Text file. The best test is to be able to view > this file using Microsoft's Notepad for Windows 3.x or Wordpad for > Windows '95. If the margins look right without making the screen slide > left or right when at the ends of the sentences, you're in business! > > 5. If you need help uploading, simply ask the sysop of the BBS, or > "POST" a message on a newsgroup asking how to post a file, tell them > who your Internet provider is and PEOPLE WILL ALWAYS BE GLAD TO HELP. > I would try to describe how to do it but there are simply too many > internet software packages with slighty different yet relatively > simple ways to post or upload a file. Just ask for help or look in the > help section for 'posting'. I do know that for GNN, you simply select > 'POST' then enter a catchy description under the subject box, choose > 'ATTACH', selecting 'UUE' and NOT 'TXT', then choose 'Browse' to go > look for the file. Find your text file FASTCASH.TXT and click on it > and choose 'OK'. Place a one line statement in the main body section > of the message post screen. Something like "Download this to read how > to get cash arriving in your mailbox with no paybacks!" or something > to that affect. Just make sure it > represents its true feasiblity, NOT something like... "Get one million > dollars flooding in your mailbox in two days!" You'll never get ANY > responses! > > 6. And this is the step I like. JUST SIT BACK AND ENJOY LIFE BECAUSE > CASH IS ON ITS WAY!! Expect to see a little money to trickle in around > 2 weeks, but AT ABOUT WEEK 3 & 4, THE MONEY STORM WILL HIT YOR > MAILBOX!! All you have to do is take it out of the mailbox and try not > to scream too load (outside anyway) when you realize YOU HIT THE BIG > TIME AT LAST!! > > 7. So go PAY OFF YOUR BILLS AND DEBTS and then get that something > special > you always wanted or buy that special person in your life (or the one > you want in your life) a gift they'll never forget. ENJOY LIFE! > > 8. Now when you get low on this money supply, simply re-activate this > file again; Reposting it in the old places where you originally posted > and possibly some new places you now know of. Don't ever lose this > file, always keep a copy at your reach for when you ever need > fastcash. THIS IS AN INCREDIBLE TOOL THAT YOU CAN ALWAYS RE-USE TIME > AND TIME AGAIN WHEN FASTCASH IS NEEDED. Think about it, if used > correctly return for so little an investment? Good luck and give this > plan your all, it will definitely pay off! HAVE FUN WITH IT!!! > > > **************************************************************************** > THE NAMES LIST THE NAMES LIST THE NAMES LIST > **************************************************************************** > * HONESTY IS WHAT MAKES THIS PROGRAM SUCCESSFUL!!! > * > * #1. C. PRUETT > * PO BOX 1047 > * ANOKA MN. 55303 > * USA > * > * #2 SUZIE COOK > * 1820 west sahuaro #110 > * phoenix az. 85029 > * USA > * > * #3 W. PRUETT > * 368 E. SCHOOL ST. #2 > * OWATONNA MN. 55060 > * USA > * > * #4 DONNA GLENN > * 308 3RD AVE EAST > * JEROME, ID 83338 > * USA > * > * #5 Matt Marshall > * 17213 21st AVE SE > * Bothell, WA 98012 > * USA > ****************************************************************************** > > From owner-structural-nmr@net.bio.net Thu Nov 21 22:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.structural-nmr Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 22 Nov 1996 02:00:42 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Distribution: world Message-ID: <199611221000.CAA19788@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-structural-nmr@net.bio.net Sat Nov 23 22:00:00 1996 Path: biosci!agate!howland.erols.net!news-peer.gsl.net!news.gsl.net!news-paris.gsl.net!news.gsl.net!rain.fr!speedy.grolier.fr!usenet From: androide Newsgroups: bionet.structural-nmr Subject: DROIDE-CLUB search? Date: Sun, 24 Nov 1996 13:37:57 +0100 Organization: DROIDE-CLUB Lines: 8 Message-ID: <329841A5.1AFF@club-internet.fr> Reply-To: androide@club-internet.fr NNTP-Posting-Host: ppp-196-241.neuilly.club-internet.fr Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Mailer: Mozilla 3.0 (Win95; I) I search a new matérial (polymeres sous forme de gels durs) to replace actuators in the conception of droide (not robot). Please can you help me to find some informations about this subject. -- Le président de DROÏDE-CLUB M. Alain BARRET 78180 ST. Quentin En Yvelines France androide@club-internet.fr http://web.club-internet.fr/androide From owner-structural-nmr@net.bio.net Sun Nov 24 22:00:00 1996 Path: biosci!PETER.BPC.UNI-FRANKFURT.DE!micha From: micha@PETER.BPC.UNI-FRANKFURT.DE (Michael Marek) Newsgroups: bionet.structural-nmr Subject: NMR position in Frankfurt Date: 25 Nov 1996 13:08:43 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 53 Sender: daemon@net.bio.net Distribution: world Message-ID: <329A17F2.41C6@bpc.uni-frankfurt.de> NNTP-Posting-Host: net.bio.net

The Frankfurt University Centre for Biomolecular NMR is part of a European Union supported Large Scale Facility, under the terms of the Training and Mobility of Researchers Programme, which is operated as a facility for European scientists. Leading scientists of the Centre are Prof. Rüterjans and Prof. Griesinger of the Institute of Biophysical Chemistry and the Institute of Organic Chemistry of the University of Frankfurt.

Within the NMR group of Prof. Rüterjans a vacancy exists for the post of a

Supervising Scientist

This scientist will provide the scientific expertise and will be responsible for training, assisting and advising visiting scientists from European laboratories. A suitable candidate will have the opportunity to start a NMR research project in structural biology of his own or within the context of the research programme of the institute. In addition there will be ample opportunities to collaborate with visiting scientists using the 800, 600 or 500 MHz NMR spectrometers of the NMR Large Scale Facility. Together with a second specialist the scientist will be responsible for the day to day management, operation and reporting of the Large Scale Facility.

The applicant will have a Ph.D. degree, a good theoretical knowledge of advanced NMR techniques, extensive research experience in modern high field NMR spectroscopy as applied to problems in strucural biology, a good working knowledge of modern NMR spectrometers and the associated NMR software packages. Experience with NMR based structure calculations will be an advantage. The applicant should have good interpersonal skills and flexibility and be willing to operate in close cooperation with users of the facility.

The appointment will be for the duration of the project (at least two years) with a salary indication of DM 80.000,- - Dm 95.000,- per year (BAT) depending on experience. The position is available from January 1st, 1997.

Applications, including a full CV with a list of publications and the names, phone numbers and addresses of three possible referees, should be sent wihin three weeks from the date of this issue to:

Prof. Dr.Heinz Rüterjans
Institute of Biophysical Chemistry
J.W. Goethe University Frankfurt
Marie-Curie-Str. 9
D-60439 Frankfurt/Main
Germany
Fax: +49.69.79829632
Phone: +49.69.79829631
email: hruet@peter.bpc.uni-frankfurt.de

From owner-structural-nmr@net.bio.net Mon Nov 25 22:00:00 1996 Path: biosci!agate!howland.erols.net!news.sgi.com!esiee.fr!jussieu.fr!univ-angers.fr!univ-rennes1.fr!news From: Doctorants.seso-mv@univ-rennes1.fr (Jean-Louis Toujas) Newsgroups: bionet.structural-nmr Subject: which PRINTER for a Brueker AC300 ? Date: 26 Nov 1996 14:25:48 GMT Organization: Universite de Rennes 1 Lines: 9 Message-ID: <57eulc$6t6@news.univ-rennes1.fr> NNTP-Posting-Host: pc-rmn.univ-rennes1.fr X-Newsreader: WinVN 0.92.2 Would anybody know if it is possible to install an INK-JET PRINTER (A3 format) on a BRUEKER AC300 running with an ASPECT 3000 computer. Wich one would be the most preferable or at least the most compatible for this purpose? Thank you for your help cordialement, Jean-Louis Toujas From owner-structural-nmr@net.bio.net Mon Nov 25 22:00:00 1996 Path: biosci!rutgers!uwm.edu!cs.utexas.edu!howland.erols.net!surfnet.nl!swidir.switch.ch!news.belwue.de!news-stu1.dfn.de!news-kar1.dfn.de!news.rwth-aachen.de!newsserver.rrzn.uni-hannover.de!tubsibr!news From: i3080419@ws.rz.tu-bs.de (W.Schuetze) Newsgroups: bionet.structural-nmr Subject: 31 P - NMR of Biomembranes Date: 26 Nov 1996 16:34:54 +0100 Organization: Technische Universitaet Braunschweig, Germany Lines: 23 Sender: i3080419@rz1pht1.rz.tu-bs.de Distribution: world Message-ID: NNTP-Posting-Host: rz1pht1.rz.tu-bs.de X-Newsreader: Gnus v5.2.40/Emacs 19.34 Hi, I am looking for basic literature on 31 P-NMR of phospholipid layers/membranes. Is there something that one should read first? Thanks in advance, Wolfgang -- +-----------------------------------------------------------------------+ | Wolfgang Schuetze, Institut fuer Pharmazeutische Technologie | | Technische Universitaet Carolo-Wilhelmina zu Braunschweig | | email: w.schuetze@tu-bs.de Tel. 0531-391-5654 | | www : http://www.tu-bs.de/institute/pharmtech/pht/wo.schuetze.html | +-----------------------------------------------------------------------+ From owner-structural-nmr@net.bio.net Tue Nov 26 22:00:00 1996 Path: biosci!jk.uni-linz.ac.at!Alexej.Jerschow From: Alexej.Jerschow@jk.uni-linz.ac.at (Alexej Jerschow) Newsgroups: bionet.structural-nmr Subject: TPPI, what type of FFT ? Date: 27 Nov 1996 03:31:56 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 38 Sender: daemon@net.bio.net Distribution: world Message-ID: <199611271110.MAA00749@OClinux1.chemie.uni-linz.ac.at> NNTP-Posting-Host: net.bio.net Dear NMR Spectroscopists! I have problems with figuring out how to FFT TPPI-acquired data. I try to do it myself (without the spectrometer software) on simulated data. I have some conceptual difficulty understanding what kind of FFT I should use. My simulated data (1D) is: fid(t)=[exp(-i*t*omega)+exp(i*t*omega)]*exp(-t/T2) where the 1st term in is I+ coherence and the second I- (or the other way round). The fid is now amplitude modulated, the imaginary parts cancel. When I do a complex (ordinary) FFT I get 2 peaks, one at +omega and one at -omega corresponding to the 2 coherences. Using TPPI (increment the pulse phase before evolution by 90 deg. wrt the previous point) I have to phase shift the I- coherence by -90 and the I+ coherence by +90 (or the other way round). In this way the 2 peaks are moved by f(Nyquist)/2 to the left and right, respectively. This I can observe by complex FFT again. The books say that I have to use a *real* FFT to get the 2 coherences folded onto each other in a way that only absorptive signals remain. But when I use a *real* FFT (which is a cosine transform in my interpretation) I get the same pattern, i.e. 2 peaks. (the same happens when I use a sine transform). Which FFT should I use, or where did I make an error ? Thank you Alexej Jerschow PS: I encountered the same problem when trying to simulate the States method: Every other point shifted by 90 deg (the pulse phase), then combine two points as real and imaginary parts. Complex FFT should yield the desired result but it did not in my case. From owner-structural-nmr@net.bio.net Tue Nov 26 22:00:00 1996 Path: biosci!PETER.BPC.UNI-FRANKFURT.DE!micha From: micha@PETER.BPC.UNI-FRANKFURT.DE (Michael Marek) Newsgroups: bionet.structural-nmr Subject: NMR position in Frankfurt Date: 27 Nov 1996 00:40:40 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 44 Sender: daemon@net.bio.net Distribution: world Message-ID: <329C0C0C.41C6@bpc.uni-frankfurt.de> NNTP-Posting-Host: net.bio.net The Frankfurt University Centre for Biomolecular NMR is part of a European Union supported Large Scale Facility, under the terms of the Training and Mobility of Researchers Programme, which is operated as a facility for European scientists. Leading scientists of the Centre are Prof. Ruterjans and Prof. Griesinger of the Institute of Biophysical Chemistry and the Institute of Organic Chemistry of the University of Frankfurt. Within the NMR group of Prof. Ruterjans a vacancy exists for the post of a Supervising Scientist This scientist will provide the scientific expertise and will be responsible for training, assisting and advising visiting scientists from European laboratories. A suitable candidate will have the opportunity to start a NMR research project in structural biology of his own or within the context of the research programme of the institute. In addition there will be ample opportunities to collaborate with visiting scientists using the 800, 600 or 500 MHz NMR spectrometers of the NMR Large Scale Facility. Together with a second specialist the scientist will be responsible for the day to day management, operation and reporting of the Large Scale Facility. The applicant will have a Ph.D. degree, a good theoretical knowledge of advanced NMR techniques, extensive research experience in modern high field NMR spectroscopy as applied to problems in strucural biology, a good working knowledge of modern NMR spectrometers and the associated NMR software packages. Experience with NMR based structure calculations will be an advantage. The applicant should have good interpersonal skills and flexibility and be willing to operate in close cooperation with users of the facility. The appointment will be for the duration of the project (at least two years) with a salary indication of DM 80.000,- - DM 95.000,- per year (BAT) depending on experience. The position is available from January 1st, 1997. Applications, including a full CV with a list of publications and the names, phone numbers and addresses of three possible referees, should be sent wihin three weeks from the date of this issue to: Prof. Dr.Heinz Ruterjans Institute of Biophysical Chemistry J.W. Goethe University Frankfurt Marie-Curie-Str. 9 D-60439 Frankfurt/Main Germany Fax: +49.69.79829632 Phone: +49.69.79829631 email: hruet@peter.bpc.uni-frankfurt.de From owner-structural-nmr@net.bio.net Tue Nov 26 22:00:00 1996 Path: biosci!agate!howland.erols.net!spool.mu.edu!usenet.eel.ufl.edu!warwick!lyra.csx.cam.ac.uk!news.ox.ac.uk!news From: liz Newsgroups: bionet.structural-nmr Subject: NMR Discussion Meeting Date: Wed, 27 Nov 1996 11:13:13 +0100 Organization: Oxford University Lines: 4 Message-ID: <329C1428.31E@bioch.ox.ac.uk> NNTP-Posting-Host: raleigh.bioch.ox.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (Macintosh; I; PPC) A Harden Discussion Meeting entitled "NMR of Macromolecules - The Future" will be held at Oxford University, UK from 13-16 April 1997. Further details are on the web site http://www.bioch.ox.ac.uk/~eliz/Harden/meeting.html From owner-structural-nmr@net.bio.net Tue Nov 26 22:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.erols.net!feed1.news.erols.com!phase2.worldnet.att.net!uunet!in3.uu.net!ott.istar!istar.net!news.nstn.ca!news.dal.ca!chebucto.ns.ca!ai522 From: ai522@chebucto.ns.ca (Ian Young) Newsgroups: bionet.structural-nmr Subject: High End 1H NMR spectra databases Date: 27 Nov 1996 16:41:53 GMT Organization: Chebucto Community Net Lines: 8 Message-ID: <57hr0h$hs5@News.Dal.Ca> NNTP-Posting-Host: chebucto.ns.ca X-Newsreader: TIN [version 1.2 PL2] What databases are available of proton nmr specta at high fields (e.g 300 MHz)? I have found the Merck database http://www.intaccess.com/fdm/pnmr.htm and Advanced Chemistry Development's ACD/HNMR database: http://www.acdlabs.com/products/hnmr/ and am aware of the Beilstein and HODOC databases on STN. Are there any others? -- From owner-structural-nmr@net.bio.net Thu Nov 28 22:00:00 1996 Path: biosci!agate!howland.erols.net!news.mathworks.com!usenet.eel.ufl.edu!warwick!lyra.csx.cam.ac.uk!hgmp.mrc.ac.uk!news From: Mike Gradwell Newsgroups: bionet.structural-nmr,sci.techniques.mag-resonance Subject: Shigemi tubes Date: Fri, 29 Nov 1996 16:23:21 +0100 Organization: National Institute for Medical Research Lines: 20 Message-ID: <329EFFE9.41C67EA6@nimr.mrc.ac.uk> NNTP-Posting-Host: nmrsn44.nimr.mrc.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.01 (X11; I; SunOS 4.1.3 sun4m) Xref: biosci bionet.structural-nmr:1620 sci.techniques.mag-resonance:1863 Dear Netters, I've recently purchased sets of 5mm and 8mm Shigemi tubes for use with Varian spectrometers from a 3rd party European supplier (Campro Scientific). The tubes come with no guidelines for use. When attempting to make up test samples we have a problem - a bubble becomes trapped at the top of the sample under the upper piece of susceptibility matched material. There seems no easy way of avoiding or remedying this problem - you can't squeeze the bubble out on the bottom, as this forces solution out of the top of the tube and tapping and shaking the tube seems to have little efect (at least after about 15 minutes of doing this!!!!) Does anybody have any guidelines for useage? Mike Gradwell. -- Dr. Mike Gradwell, MRC Biomedical NMR Centre, National Institute for Medical Research, Mill Hill, London NW7 1AA. Tel. +44181 959 3666 ext. 2026 Fax. +44181 906 4477 From owner-structural-nmr@net.bio.net Thu Nov 28 22:00:00 1996 Path: biosci!daresbury!lyra.csx.cam.ac.uk!nntpfeed.doc.ic.ac.uk!sunsite.doc.ic.ac.uk!dundee.ac.uk!CHEM1.chem.dundee.ac.uk!fmohdsom From: fmohdsom@its.dundee.ac.uk (FAUZI MOHDSOM) Newsgroups: bionet.structural-nmr Subject: T1rho Date: Fri, 29 Nov 1996 16:10:38 GMT Organization: University of Dundee Lines: 9 Message-ID: NNTP-Posting-Host: chem1.chem.dundee.ac.uk X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] I have found that several papers have interchangeably used the word C-13 T1rho and H-1 T1rho on the same type experiment which is by varying contact time during CP. Several of the recent papers have used the time constant obtained as C-13 T1rho however paper by Schaefer back in 1977 called that time constant as H-1 T1rho. Can anybody help me to clarify this matter? Thank you. From owner-structural-nmr@net.bio.net Fri Nov 29 22:00:00 1996 Path: biosci!agate!howland.erols.net!news.bbnplanet.com!cam-news-hub1.bbnplanet.com!news.mathworks.com!uunet!in1.uu.net!206.114.192.132!news.dmnd.net!opus.anet-stl.com!usenet From: Bill & Kay Johnson Newsgroups: bionet.structural-nmr,sci.techniques.mag-resonance Subject: Re: Shigemi tubes Date: Sat, 30 Nov 1996 08:43:15 -0800 Organization: Western Pacific Network Services Lines: 32 Message-ID: <32A06423.1346@anet-stl.com> References: <329EFFE9.41C67EA6@nimr.mrc.ac.uk> NNTP-Posting-Host: ppp32.anet-stl.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.01 (Win16; U) Xref: biosci bionet.structural-nmr:1622 sci.techniques.mag-resonance:1865 Mike Gradwell wrote: > > Dear Netters, > I've recently purchased sets of 5mm and 8mm Shigemi tubes > for use with Varian spectrometers from a 3rd party European supplier > (Campro Scientific). The tubes come with no guidelines for use. > When attempting to make up test samples we have a problem - a bubble > becomes trapped at the top of the sample under the upper piece of > susceptibility matched material. There seems no easy way of avoiding > or remedying this problem - you can't squeeze the bubble out on > the bottom, as this forces solution out of the top of the tube and > tapping and shaking the tube seems to have little efect (at least after > about 15 minutes of doing this!!!!) > Does anybody have any guidelines for useage? > Mike Gradwell. > -- > Dr. Mike Gradwell, > MRC Biomedical NMR Centre, > National Institute for Medical Research, > Mill Hill, > London NW7 1AA. > Tel. +44181 959 3666 ext. 2026 Fax. +44181 906 4477 Mike- Have you tried sonicating the specimen? I have found this to be effective at times. -- Bill & Kay Johnson --------------------- bkj@anet-stl.com http://webusers.anet-stl.com/~bkj From owner-structural-nmr@net.bio.net Fri Nov 29 22:00:00 1996 Path: biosci!agate!jacek.hip.berkeley.edu!user From: jacek@lcbvax.cchem.berkeley.edu (Jacek Nowakowski) Newsgroups: bionet.structural-nmr,sci.techniques.mag-resonance Subject: Re: Shigemi tubes Date: 30 Nov 1996 21:09:05 GMT Organization: UC Berkeley Lines: 36 Message-ID: References: <329EFFE9.41C67EA6@nimr.mrc.ac.uk> NNTP-Posting-Host: jacek.hip.berkeley.edu X-Newsreader: Yet Another NewsWatcher 2.1.5 Xref: biosci bionet.structural-nmr:1623 sci.techniques.mag-resonance:1866 In article <329EFFE9.41C67EA6@nimr.mrc.ac.uk>, Mike Gradwell wrote: > Dear Netters, > I've recently purchased sets of 5mm and 8mm Shigemi tubes > for use with Varian spectrometers from a 3rd party European supplier > (Campro Scientific). The tubes come with no guidelines for use. > When attempting to make up test samples we have a problem - a bubble > becomes trapped at the top of the sample under the upper piece of > susceptibility matched material. There seems no easy way of avoiding > or remedying this problem - you can't squeeze the bubble out on > the bottom, as this forces solution out of the top of the tube and > tapping and shaking the tube seems to have little efect (at least after > about 15 minutes of doing this!!!!) > Does anybody have any guidelines for useage? > Mike Gradwell. Mike, I have used the Shigemi tubes several times and this is what worked for me: To remove to bubble, push the piston ALL the way to the bottom (of course as you noticed the volume of the sample must be small enough not to overflow out of the tube - I normally use 180-230 uL). This will not get rid of the bubble entirely because some of it will get trapped underneath the piston. Then move the piston up a little bit and tap it down. What you're trying to do is to use liquids turbulence to push the air out. After several trials all of the bubble should be gone. This method worked for me every time - if it still doesn't work for you, try a different tube. Jacek P.S. Sometimes the bubble isn't so bad for the quality of your spectrum. If you can't get rid of it - put it in the magnet anyway and see what the data looks like. From owner-structural-nmr@net.bio.net Sat Nov 30 22:00:00 1996 Path: biosci!PICASSO.UCSF.EDU!mujeeb From: mujeeb@PICASSO.UCSF.EDU (Anwer MUJEEB) Newsgroups: bionet.structural-nmr Subject: Re: Shigemi tubes Date: 30 Nov 1996 16:00:46 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 48 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <32A06423.1346@anet-stl.com> NNTP-Posting-Host: net.bio.net Hi: The best way to deal with the bubble is to give a quick, light but strong tap on the plunger. Just make sure you have only one bubble close to surface i.e. touching the plunger and give a brisk tap. For us, it works all the time, and that is what shigemi people suggested during last ENC. -anwer mujeeb UCSF NMR group San Franscisco. On Sat, 30 Nov 1996, Bill & Kay Johnson wrote: > Mike Gradwell wrote: > > > > Dear Netters, > > I've recently purchased sets of 5mm and 8mm Shigemi tubes > > for use with Varian spectrometers from a 3rd party European supplier > > (Campro Scientific). The tubes come with no guidelines for use. > > When attempting to make up test samples we have a problem - a bubble > > becomes trapped at the top of the sample under the upper piece of > > susceptibility matched material. There seems no easy way of avoiding > > or remedying this problem - you can't squeeze the bubble out on > > the bottom, as this forces solution out of the top of the tube and > > tapping and shaking the tube seems to have little efect (at least after > > about 15 minutes of doing this!!!!) > > Does anybody have any guidelines for useage? > > Mike Gradwell. > > -- > > Dr. Mike Gradwell, > > MRC Biomedical NMR Centre, > > National Institute for Medical Research, > > Mill Hill, > > London NW7 1AA. > > Tel. +44181 959 3666 ext. 2026 Fax. +44181 906 4477 > > Mike- > Have you tried sonicating the specimen? I have found this to be > effective at times. > > -- > Bill & Kay Johnson > --------------------- > bkj@anet-stl.com > http://webusers.anet-stl.com/~bkj > >