From owner-structural-nmr@net.bio.net Thu May 01 23:00:00 1997 Path: biosci!POUND.MED.UTORONTO.CA!vincent From: vincent@POUND.MED.UTORONTO.CA (Sebastien Vincent) Newsgroups: bionet.structural-nmr Subject: Re: help Date: 2 May 1997 04:43:24 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: <199705021143.HAA20612@pines.med.utoronto.ca> NNTP-Posting-Host: net.bio.net > From murield@PASTEUR.FR Thu May 1 17:32:44 1997 > To: str-nmr@net.bio.net > From: murield@PASTEUR.FR (Muriel DELEPIERRE) > Subject: help > We need some help with a virus introduced on our MacIntosh > probably through attached mail. This affect only word 6 and stopped us to > "register under" all the word icone of the corrupted files appears with an > arrow. It seems to be linked with macros functions . How to get rid of > this? is there an antivirus available? Any help will be gratefull Hi, Look at the www pages below which describe Word6 (Macro or Prank or others) viruses and how to get rid of them. A company called Symantec offers a virus protection program called SAM 4.5 which hopefully cures this kind of condition, with frequent updates on the latest viral strains (check out http://www.symantec.com/cgi-bin/menu.cgi). Or you can try out Micr0s0ft's technical support (http://www.microsoft.com/kb/softlib/) listing the files concerning Word6. Good luck, Sebastien http://www.gseis.ucla.edu/etu/howto/macrovirus/macrovirus.html http://saturn.med.nyu.edu/~timc/virus/cleanmac.html http://civil.ce.utexas.edu/docs/lrc/sw/wordmacro/wordmacro.html http://lipsmac.acs.unt.edu/virus/ (::) (::) (::) (::) (::) (::) (::) (::) (::) (::) (::) (::) (::) (::) Sebastien Vincent University of Toronto vincent@redfield.med.utoronto.ca Department of Medical Genetics, Voice: ++(416) 978-0642 1, King's College Circle, Fax: ++(416) 978-6885 Toronto, Ontario, CANADA M5S 1A8 www: http://abragam.med.utoronto.ca/~vincent/ "I need something to fly over my gravity" (M. Stipes) From owner-structural-nmr@net.bio.net Thu May 01 23:00:00 1997 Path: biosci!PRINCETON.EDU!wwang From: wwang@PRINCETON.EDU (Wei Wang) Newsgroups: bionet.structural-nmr Subject: subscribe Date: 2 May 1997 14:35:26 -0700 Organization: Princeton University Lines: 3 Sender: daemon@net.bio.net Distribution: world Message-ID: <336A50D2.2781@princeton.edu> NNTP-Posting-Host: net.bio.net subscribe From owner-structural-nmr@net.bio.net Thu May 01 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!europa.clark.net!newsfeed2!rill.news.pipex.net!pipex!oleane!jussieu.fr!univ-angers.fr!ciril.fr!u-strasbg.fr!news From: simonson@schlitz.u-strasbg.fr (Thomas Simonson) Newsgroups: bionet.structural-nmr Subject: Protein Engineering Symposium Date: 2 May 1997 13:35:30 GMT Organization: CRC - Universite Louis Pasteur - Strasbourg France Lines: 54 Message-ID: <5kcqj2$b1@news.u-strasbg.fr> NNTP-Posting-Host: schlitz.u-strasbg.fr Dear Colleagues, We are pleased to announce a one-day symposium in Strasbourg, May 30th, 1997, on "Protein Engineering: from Experiment to Theory and Back". There are no registration formalities or fee. The program follows. For more information send e-mail to koehl@bali.u-strasbg.fr. We look forward to seeing you. Yours sincerely, Patrice Koehl & Tom Simonson Program: 9:45--10:00 Introduction 10:00--11:00 A. Sali (Rockefeller University) Comparative protein structure modelling for genome projects. 11:00--12:00 H. Hellinga (Duke University) Rational protein design: theory, experiments and applications. Break 14:00--15:00 D. Moras (IGBMC, Strasbourg) Protein--ligand interactions in nuclear receptors of steroid hormones. 15:00--16:00 R. Glockshuber (ETH, Zurich) Random circular permutation of proteins: a new approach to study protein folding. Break 16:30--17:30 M. Karplus (Harvard and Strasbourg Universities) Molecular recognition: free energy simulations and combinatorial ligand design. ----------------------------------------------------------- Thomas Simonson Laboratoire de Biologie Structurale, C.N.R.S., Strasbourg e-mail: simonson@zinfandel.u-strasbg.fr From owner-structural-nmr@net.bio.net Thu May 01 23:00:00 1997 Newsgroups: bionet.structural-nmr Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!ais.net!uunet!in1.uu.net!192.35.48.11!hearst.acc.Virginia.EDU!murdoch!faraday.clas.Virginia.EDU!jj8a From: jj8a@faraday.clas.Virginia.EDU ([31mThe Brewer [37m) Subject: Attention Depakers.. X-Nntp-Posting-Host: faraday.clas.virginia.edu Message-ID: Sender: usenet@murdoch.acc.Virginia.EDU Organization: University of Virginia X-Newsreader: TIN [version 1.2 PL1] Date: Fri, 2 May 1997 12:26:03 GMT Lines: 15 I have modified the Depaking program originally written by Edward Sternin from Myer Bloom's Lab to read in and write out Felix ACSII files. I'll be glad to provide the Felix Macro and the menu files to any one who is interested. The Felix Macro actually calls the fortran program and hence you'll have to work out the permission and copyright agreements with Dr. Bloom. As far as I know it is freely available to interested users. -- Jaison Jacob Chemistry Department Univ. of Virginia From owner-structural-nmr@net.bio.net Thu May 01 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!news.maxwell.syr.edu!news-feed.inet.tele.dk!uninett.no!news.uni-c.dk!news.uni-c.dk!ssv2.dina.kvl.dk!not-for-mail From: Jesper Broendum Nielsen Newsgroups: bionet.structural-nmr,bionet.molec-model,sci.med.laboratory,bionet.biophysics,sci.physics Subject: Announcement: NMR Database at http://newton.foodsci.kvl.dk Date: Thu, 01 May 1997 09:45:36 +0200 Organization: Royal Veterinary & Agricultural U., Copenhagen, Denmark Lines: 27 Message-ID: <33684A20.41C6@newton.foodsci.kvl.dk> NNTP-Posting-Host: newton.foodsci.kvl.dk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (X11; I; IRIX 5.2 IP22) CC: jesper Xref: biosci bionet.structural-nmr:1910 bionet.molec-model:1518 sci.med.laboratory:3823 bionet.biophysics:2915 sci.physics:210752 Dear colleges We have put together a database of publications (incl. abstracts) in low resolution NMR. The database is separated in two groups of applications: - Food Applications - Related Applications Visit: http://newton.foodsci.kvl.dk best regards -- ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Jesper Broendum, M.Sc. Image Analysis Food Technology and SFK-Technology A/S Vet. and Agri. Uni. Rolighedsvej 30 Vandtaarnsvej 77 1958 Frederiksberg C 2860 Soeborg, DK Ph: (+45) 3528 3205 (+45) 3167 1527 Fax: (+45) 3528 3245 (+45) 3167 9227 E-mail: jesper@newton.foodsci.kvl.dk WWW: newton.foodsci.kvl.dk/jesper ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ From owner-structural-nmr@net.bio.net Thu May 01 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news-feed.inet.tele.dk!uninett.no!news.uni-c.dk!news.uni-c.dk!ssv2.dina.kvl.dk!not-for-mail From: Jesper Broendum Nielsen Newsgroups: bionet.structural-nmr Subject: NMR database Date: Fri, 02 May 1997 12:57:51 +0200 Organization: Royal Veterinary & Agricultural U., Copenhagen, Denmark Lines: 27 Message-ID: <3369C8AF.41C6@newton.foodsci.kvl.dk> NNTP-Posting-Host: newton.foodsci.kvl.dk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (X11; I; IRIX 5.2 IP22) Dear Colleges, We have put together a database of publications (incl. abstracts) in low resolution NMR. The database is separated in two groups of applications: - Food Applications - Related Applications Visit: http://newton.foodsci.kvl.dk best regards -- ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Jesper Broendum, M.Sc. Image Analysis Food Technology and SFK-Technology A/S Vet. and Agri. Uni. Rolighedsvej 30 Vandtaarnsvej 77 1958 Frederiksberg C 2860 Soeborg, DK Ph: (+45) 3528 3205 (+45) 3167 1527 Fax: (+45) 3528 3245 (+45) 3167 9227 E-mail: jesper@newton.foodsci.kvl.dk WWW: newton.foodsci.kvl.dk/jesper ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ From owner-structural-nmr@net.bio.net Thu May 01 23:00:00 1997 Path: biosci!NMR1.BCH.MSU.EDU!gao From: gao@NMR1.BCH.MSU.EDU (Jinhai Gao) Newsgroups: bionet.structural-nmr Subject: nmrview help Date: 1 May 1997 18:08:20 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: <33693EFE.2781@nmr1.bch.msu.edu> NNTP-Posting-Host: net.bio.net Dear Netters, I am trying to plot the overlayed colour HSQC spectra from NMRVIEW. But I failed to do this. Does anybody know if NMRVIEW can do this? or any other methods/software can do this? In addition, I also tro to analyze 3D NMR data and NOE assignment using NMRVIEW, but i donot know what NMRVIEW can do about this? Is it better than others (Felix950, Xeasy, or Pronto)? I am not sure! Actually I cannot know more from the NMRVIEW manual about these things and I am afraid it is not easy to figure them out myself without any help or instruction. I sent several mails to both NMRVIEW email list (nmrview-request@) and the author, but I never get any response. Any help or suggestions will be appreciated very much! Jinhai From owner-structural-nmr@net.bio.net Sun May 04 23:00:00 1997 Path: biosci!IRIS1.SB.FSU.EDU!logan From: logan@IRIS1.SB.FSU.EDU (Tim Logan) Newsgroups: bionet.structural-nmr Subject: Postdoctoral Position(s) Available Date: 5 May 1997 13:40:55 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 53 Sender: daemon@net.bio.net Distribution: world Message-ID: <9705052041.AA14481@iris1.sb.fsu.edu> NNTP-Posting-Host: net.bio.net Colleagues: I am currently seeking qualified candidates for two postdoctoral positions in my lab at the Institute of Molecular Biophysics at Florida State University, and at the National High Magnetic Field Laboratory. One fellow will participate in ongoing research directed towards determining the three-dimensional solution structures of proteins using multi-dimensional heteronuclear NMR spectroscopy. There are several projects at different stages of completion ranging from data collection to optimizing expression and designing purification schemes. Individuals with experience in various aspects of protein NMR spectroscopy, including molecular biology, protein chemistry, data collection and analysis, and structure calculation, will be considered. The other fellow will participate in research combining spectroscopy (NMR, CD, and fluorescence) with mutagenesis to investigate the role of the unfolded state in protein folding and stability. Candidates with experience in protein chemistry and protein folding are encouraged to apply. The Institute is home to the Program in Structural Biology at FSU and houses state-of-othe-art laboratory facilities for bacterial, mammalian, and insect cell culture, molecular biology, protein purification, and computation / structure determination. The Magnet Lab is an NSF-supported National Lab dedicated to research and development of magnetic phenomena at very high fields. NMR experiments are performed at the NHMFL using 500 and 720 MHz NMR spectrometers equipped for triple- and quadruple-resonance experiments with PFG and shaped-pulse capabilities. We are currently installing a 400 MHz triple-resonance spectrometer purchased for the development of high temperature super-conducting probes. Furthermore, a 900 MHz NMR spectrometer will be completed in 1998 and devoted to high resolution NMR studies of biomolecules. The NHMFL also contains excellent EMR and ICR facilities and fosters strong interactions between the various magnetic resonance disciplines through the Center for Interdisciplinary Magnetic Resonance. I would appreciate your help in identifying outstanding candidates for the position I have available. I can be contacted at the address listed below, by phone at (904) 644-8979, or by e-mail at logan@sb.fsu.edu. Thank you for your help in this matter. Best Regards, Tim Logan _______________________________ Timothy M. Logan Institute of Molecular Biophysics Florida State University Tallahassee, FL. 32306-3015 Phone: (904) 644-8979 Fax: (904) 561-1406 From owner-structural-nmr@net.bio.net Tue May 06 23:00:00 1997 Path: biosci!NMRSGI2.NCIFCRF.GOV!rabyrd From: rabyrd@NMRSGI2.NCIFCRF.GOV (R. Andrew Byrd) Newsgroups: bionet.structural-nmr Subject: Job Opportunity Date: 7 May 1997 14:26:54 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 42 Sender: daemon@net.bio.net Distribution: world Message-ID: <199705072113.RAA04251@nmrsgi2.ncifcrf.gov> NNTP-Posting-Host: net.bio.net RESEARCH TECHNICIAN The Macromolecular NMR Section, Macromolecular Structure Laboratory has an immediate opportunity for a creative, energetic individual to assist with the expression and purification of recombinant proteins for structural studies. Duties include purification of recombinant proteins, biosynthetic incorporation of stable NMR-active isotopes into proteins and nucleic acids, optimization of expression parameters, and construction of recombinant plasmid expression vectors. Experience with all of the following desirable: protein purification using FPLC and HPLC, SDS-PAGE and western blotting, growth and maintenance of E. coli, transformation of E. coli with plasmid DNA, purification of plasmid DNA. BS/MS degree in a chem., biochem., or biol. sci. w/ 3 yrs of lab exp. required. The individual will have the opportunity to work with the Protein Engineering Group, headed by Dr. David Waugh, in the development of new protein systems for structural and biophysical investigations. General organizationa nd management of the biochemistry component of the NMR lab is expected. Salary commensurate w/ experience. Excellent fringe benefits including medical/dental insurance, 100% paid educational assistance & matched savings plan. Please forward your resume to: ABL-Basic Research Program/RAB NCI-FCRDC P.O. Box B, Building 428 Frederick, MD 21702 EOE M/F/D/V -------------------------------------------------------------- Interested persons may also contact me directly via email for further information; however, an official application must be submitted via our personnel department. ------------------ Dr. R. Andrew Byrd Macromolecular NMR/MSL/ABL TEL: 301-846-1407 FAX: 301-846-6195 From owner-structural-nmr@net.bio.net Tue May 06 23:00:00 1997 Newsgroups: bionet.structural-nmr Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!newsfeed.nacamar.de!rill.news.pipex.net!pipex!server1.netnews.ja.net!warwick!bradford.ac.uk!leeds.ac.uk!news From: garyt@bmb.leeds.ac.uk (Gary Sheldon Thompson) Subject: subscribing to nmrview mailing list Message-ID: <3370D3CA.446B@bmb.leeds.ac.uk> NNTP-Posting-Host: bmbsgi14.leeds.ac.uk X-Mailer: Mozilla 3.01 (X11; I; IRIX 5.3 IP22) Content-Type: text/plain; charset=us-ascii Organization: Biochemistry and Molecular Biology, University of Leeds MIME-Version: 1.0 Date: Wed, 7 May 1997 20:09:53 +0100 (BST) Lines: 101 Content-Transfer-Encoding: 7bit Hi sorry to cause confusion on how to use the nmrview mailing list, I also got the e-mail address wrong [I am new to this game so please be kind to me!!!] Here are the corrections: To subscribe you send an e-mail message to majordomo@leeds.ac.uk with the single line "subscribe nmrview " (don't add the quotes ;-) Once you have subscribed and recieved a reply telling you that tou have been accepted onto the list any e-mail message you send to nmrview@leeds.ac.uk will be sent to all the members of the list and you will recieve a copy of any message sent to the list. NB I will clear up any problems due the previous erroneous e-mail address you shouldn't need to take any further action.... To get information on the list send an e-mail message to majordomo@leeds.ac.uk with the single line "info nmrview" (don't add the quotes ;-) which will return this message: <<<<< Message >>>>> THIS MAILING LIST This is the nmrview mailing list, a forum for the discussion of the nmrview nmr data processing and display software by BruceJohnson and Richard A. Blevins of Merck Research Laboratories. Nmrview is a non commercial package. SUBSCRIBING TO THE NMRVIEW LIST To subscribe to this list send an e-mail message with NO subject but the following body subscribe nmrview to majordomo@leeds.ac.uk UNSUBSCRIBING FROM THE NMRVIEW LIST Another way to unsubscribe from this list is to send an e-mail message with NO subject but the following body unsubscribe nmrview to majordomo@leeds.ac.uk WHAT IS NMRVIEW? NMRView is a program for the visualization and analysis of processed NMR data. It is intended to be able to do everything from the processeddata to the input for structure generation programs. The current version (3.0b3) now contains features that allow for processing of data, but these features are very new and should be considered experimental. Executable versions are currently available for SGI, SUN and IBM RS/6000 workstations, and Power Macintosh's running the MachTen operating system. Nmrview consists of an extendable suite of Tcl/Tk scripts along with a custom version of wish the TK windowing shell whcih includes an nmr display widget. HOW TO GET NMRVIEW There is a web site for nmrview where you can retrieve the binary distribution. The site is at: http://www.ges.com/nmrview/ THE MAINTAINER OF THIS LIST this list is maintained by (Dr) Gary S. Thompson at the Department of Biochemistry, University of Leeds. If you have problems e-mail owner-nmrview@leeds.ac.uk. Please note that this list is new and experimental, therefore please bear with any teething problems. <<<>>>> sorry for the confusion gary -- ********************************************************************* Dr. Gary S. Thompson Address: Wellcome Postdoctoral Fellow Biochemistry and Molecular Biology, Sheena Radford's Laboratory Woodhouse Lane, University of Leeds, Studying the structure and Leeds, LS2 9JT. folding of proteins by nmr. England. e-mail: garyt@bmb.leeds.ac.uk. tel: 0113 233 3134 fax: 0113 233 3167 ********************************************************************* From owner-structural-nmr@net.bio.net Tue May 06 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!news.maxwell.syr.edu!news-was.dfn.de!news-fra1.dfn.de!news-ge.switch.ch!news-zh.switch.ch!rzunews.unizh.ch!toukie From: toukie@zui.unizh.ch (Dr. S. Shapiro) Newsgroups: bionet.structural-nmr Subject: Fab or Ig X-ray xtal structure Date: 7 May 1997 14:21:10 GMT Organization: University of Zurich, Switzerland Lines: 16 Message-ID: <5kq34m$cfo@rzunews.unizh.ch> NNTP-Posting-Host: rzurs10.unizh.ch X-Newsreader: TIN [version 1.2 PL2] Dear Colleagues; I am seeking information on the X-ray (or neutron) diffraction struc- ture of the binding site of antibodies that specifically bind phenolic-type compounds (such as _but not restricted to_ 2,4-dinitrophenol). If you can provide me with any useful references, or possibly even a PDB file, kindly contact me _directly_ at toukie@zui.unizh.ch Thanks in advance to all responders. Sincerely, S. Shapiro toukie@zui.unizh.ch From owner-structural-nmr@net.bio.net Tue May 06 23:00:00 1997 Newsgroups: bionet.structural-nmr Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!howland.erols.net!cs.utexas.edu!news.eng.convex.com!newsgate.duke.edu!news-relay.ncren.net!hearst.acc.Virginia.EDU!murdoch!faraday.clas.Virginia.EDU!jj8a From: jj8a@faraday.clas.Virginia.EDU ([31mThe Brewer [37m) Subject: Re: Depakers.. X-Nntp-Posting-Host: faraday.clas.virginia.edu Message-ID: Sender: usenet@murdoch.acc.Virginia.EDU Organization: University of Virginia X-Newsreader: TIN [version 1.2 PL1] References: <33705BCF.446B@bmb.leeds.ac.uk> Date: Wed, 7 May 1997 13:16:29 GMT Lines: 27 : what is the Dpaking program please??? For a spin 1 nuclei, in a crystalline sample, an axiallys symmetric quadrupolar interaction gives rise to a NMR spectrum, which is a doublet with its splitting proportional to 3*Cos^2(theta) - 1, where theta is the angle between the symmetry axis of the electric field gradient and the external magnetic fieeld. (Note that the same kind of splitting holds good for dipolar interaction between 2 spin 1/2 nuclei, in which case theta is the angle between the vector joining the 2 nuclei and the external field). In the case of a crystalline powder, which has large number of randomly oriented domains, the superposition of the doublets arising from each of these orientation produces a characteristic lineshape dominated by a pair of peaks arising from the most probable value of theta = pi/2. This is the famous Pake doublet. Deconvoluting the oriented spectrum from this powder pattern is "Depaking". Hope this helps. I would recommend reading JMR, v55, 274-282 (1983) and Edward Sternin's Thesis for a comprhensive treatment of the numerical algorithm. Cheers Jaison From owner-structural-nmr@net.bio.net Tue May 06 23:00:00 1997 Newsgroups: bionet.structural-nmr Path: biosci!daresbury!uninett.no!news-feed.inet.tele.dk!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!howland.erols.net!rill.news.pipex.net!pipex!server1.netnews.ja.net!warwick!bradford.ac.uk!leeds.ac.uk!news From: garyt@bmb.leeds.ac.uk (Gary Sheldon Thompson) Subject: Re: nmrview help Message-ID: <337062C0.52BF@bmb.leeds.ac.uk> NNTP-Posting-Host: bmbsgi14.leeds.ac.uk X-Mailer: Mozilla 3.01 (X11; I; IRIX 5.3 IP22) Content-Type: text/plain; charset=us-ascii Organization: Biochemistry and Molecular Biology, University of Leeds MIME-Version: 1.0 Date: Wed, 7 May 1997 12:07:31 +0100 (BST) References: <33693EFE.2781@nmr1.bch.msu.edu> Lines: 49 Content-Transfer-Encoding: 7bit Jinhai Gao wrote: > > Dear Netters, > > I am trying to plot the overlayed colour HSQC spectra from NMRVIEW. > But I failed to do this. Does anybody know if NMRVIEW can do this? or > any other methods/software can do this? It can but the human interface is currently poor and you will most probably have to hack around with some tcl/tk to do it > In addition, I also tro to analyze 3D NMR data and NOE assignment > using NMRVIEW, but i donot know what NMRVIEW can do about this? Is it > better than others (Felix950, Xeasy, or Pronto)? I am not sure! Actually > I cannot know more from the NMRVIEW manual about these things and I am > afraid it is not easy to figure them out myself without any help or > instruction. I sent several mails to both NMRVIEW email list > (nmrview-request@) and the author, but I never get any response. > Any help or suggestions will be appreciated very much! Again this can be done in nmrview, however, there is only currently direct supprt for the assignment of hncbca spectra, so you would again have to hack some tcl/tk to use this feature. > Jinhai I am sorry to see that you had problems with the nmrview mailing list the correct address is nmrview@leeds.ac.uk (I run the list), if you have problems with reaching the list via this address please contact me at garyt@bmb.leeds.ac.uk -- ********************************************************************* Dr. Gary S. Thompson Address: Wellcome Postdoctoral Fellow Biochemistry and Molecular Biology, Sheena Radford's Laboratory Woodhouse Lane, University of Leeds, Studying the structure and Leeds, LS2 9JT. folding of proteins by nmr. England. e-mail: garyt@bmb.leeds.ac.uk. tel: 0113 233 3134 fax: 0113 233 3167 ********************************************************************* From owner-structural-nmr@net.bio.net Tue May 06 23:00:00 1997 Newsgroups: bionet.structural-nmr Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!howland.erols.net!rill.news.pipex.net!pipex!server1.netnews.ja.net!warwick!bradford.ac.uk!leeds.ac.uk!news From: garyt@bmb.leeds.ac.uk (Gary Sheldon Thompson) Subject: nmrview mailing list Message-ID: <33705EA3.3F54@bmb.leeds.ac.uk> NNTP-Posting-Host: bmbsgi14.leeds.ac.uk X-Mailer: Mozilla 3.01 (X11; I; IRIX 5.3 IP22) Content-Type: text/plain; charset=us-ascii Organization: Biochemistry and Molecular Biology, University of Leeds MIME-Version: 1.0 Date: Wed, 7 May 1997 11:49:56 +0100 (BST) Lines: 48 Content-Transfer-Encoding: 7bit This is just to advertise that the nmrview maiuling list has the following address nmrview@leeds.ac.uk if there are any problems please contact garyt@bmb.leeds.ac.uk gary nb the nmrview web site is http://www.ges.com/nmrview/ nb 2 ;-) NMRView is a comprehensive program for the visualization and analysis of processed NMR spectra. The program includes molecular structure analysis and visualization features so that spectral analysis and structure generation can be a tightly linked process. NMRView includes extensive vector manipulation features that can be used for processing data sets, however, the program is primarily oriented towards the analysis of data sets processed with other programs. One of the most useful features of NMRView is the incorporation of Tcl and the Tk Toolkit. This allows users to extensively customize and automate the program. The previous version of the program is described in detail in the Journal of Biomolecular NMR (1994, 4:603-614). -- ********************************************************************* Dr. Gary S. Thompson Address: Wellcome Postdoctoral Fellow Biochemistry and Molecular Biology, Sheena Radford's Laboratory Woodhouse Lane, University of Leeds, Studying the structure and Leeds, LS2 9JT. folding of proteins by nmr. England. e-mail: garyt@bmb.leeds.ac.uk. tel: 0113 233 3134 fax: 0113 233 3167 ********************************************************************* From owner-structural-nmr@net.bio.net Tue May 06 23:00:00 1997 Newsgroups: bionet.structural-nmr Path: biosci!daresbury!lyra.csx.cam.ac.uk!warwick!bradford.ac.uk!leeds.ac.uk!news From: garyt@bmb.leeds.ac.uk (Gary Sheldon Thompson) Subject: Re: Depakers.. Message-ID: <33705BCF.446B@bmb.leeds.ac.uk> NNTP-Posting-Host: bmbsgi14.leeds.ac.uk X-Mailer: Mozilla 3.01 (X11; I; IRIX 5.3 IP22) Content-Type: text/plain; charset=us-ascii Organization: Biochemistry and Molecular Biology, University of Leeds MIME-Version: 1.0 Date: Wed, 7 May 1997 11:37:52 +0100 (BST) References: Lines: 34 Content-Transfer-Encoding: 7bit [31mThe Brewer [37m wrote: > > I have modified the Depaking program originally written by > Edward Sternin from Myer Bloom's Lab to read in and write out > Felix ACSII files. I'll be glad to provide the Felix Macro and > the menu files to any one who is interested. The Felix Macro > actually calls the fortran program and hence you'll have to > work out the permission and copyright agreements with Dr. Bloom. > As far as I know it is freely available to interested users. > > -- > Jaison Jacob > Chemistry Department > Univ. of Virginia what is the Dpaking program please??? gary -- ********************************************************************* Dr. Gary S. Thompson Address: Wellcome Postdoctoral Fellow Biochemistry and Molecular Biology, Sheena Radford's Laboratory Woodhouse Lane, University of Leeds, Studying the structure and Leeds, LS2 9JT. folding of proteins by nmr. England. e-mail: garyt@bmb.leeds.ac.uk. tel: 0113 233 3134 fax: 0113 233 3167 ********************************************************************* From owner-structural-nmr@net.bio.net Tue May 06 23:00:00 1997 Path: biosci!cnrs-orleans.fr!sodano From: sodano@cnrs-orleans.fr (Sodano Patrick) Newsgroups: bionet.structural-nmr Subject: (none) Date: 7 May 1997 00:58:56 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: <199705070758.JAA22231@admin.cnrs-orleans.fr> NNTP-Posting-Host: net.bio.net subscribe ************************************************************************* Dr Sodano Patrick Centre de Biophysique Mol=E9culaire Rue Charles Sadron 45071 Orl=E9ans C=E9dex 02 France ************************************************************************ From owner-structural-nmr@net.bio.net Thu May 08 23:00:00 1997 Path: biosci!PHOENIX.PRINCETON.EDU!ipelczer From: ipelczer@PHOENIX.PRINCETON.EDU (Istvan Pelczer) Newsgroups: bionet.structural-nmr Subject: Re: Mailing List for nmrpipe, nmrcapp, nmrpipp? Date: 9 May 1997 06:27:14 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 55 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <5kurnk$276$1@snunews.snu.ac.kr> NNTP-Posting-Host: net.bio.net Dear Hyun, For NMRPipe contact Frank Delaglio (receive password for ftp) at: Frank Delaglio for PIPP, etc., you can contact: Dan Garrett However, for data visualization and analysis I can strongly recommend NMRView, which you can learn more about at: http://www.ges.com/nmrview or/and contacting: Bruce A. Johnson If you are looking for a mailing list/discussion group, there is none for NMRPipe to my best knowledge. For getting on the NMRView mailing list send a message to majordomo@leeds.ac.uk with nothing in the subject field, and the message should only contain: subscribe nmrview . All the best, Istvan On 9 May 1997 newera@plaza.snu.ac.kr wrote: > Hi, > > What address is for nmrpipe, nmrcapp, nmrpipp mailing list? > > Oh, happy day! > > Hyun > > -- > Lee, Ji Hyun newera@plaza.snu.ac.kr > Laboratory of Physical Pharmacy(Prof. Lee, Bong Jin) > College of Pharmacy > Seoul National University > Shinlim-Dong, Kwanak-Gu > Seoul 151-742, Korea. > > Tel : 82-2-880-7869 > Fax : 82-2-872-3632 > > > From owner-structural-nmr@net.bio.net Thu May 08 23:00:00 1997 Path: biosci!MANGO.UOREGON.EDU!strain From: strain@MANGO.UOREGON.EDU (Michael Strain) Newsgroups: bionet.structural-nmr Subject: Re: Light (or at least electro-magnetic ) relief Date: 9 May 1997 13:54:42 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 134 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <3373625A.41C6@rockvax.rockefeller.edu> NNTP-Posting-Host: net.bio.net On Fri, 9 May 1997, David Cowburn wrote: > In order to dispel the rumor that all NMR people are totally without a > trace of humor, I should like to invite the structural-nmr community to > participate in a little competition. Please change the 'joke > (!@#!@%!@#)' stolen from some crystallographers, listed below, to > include one (or more )structural NMRists (probably best to avoid any > individual). Please keep it bionet.clean, and I promise to immortalize, > in some way not yet thought out, the author of the funniest (if any). > That's a dumb joke. I offer a compilation of my original puns as an alternative. --Mike ----------------------------------------------------------------------- Michael Strain strain@mango.uoregon.edu Institute of Molecular Biology desk/voice-mail: 541-346-4605 and Department of Chemistry lab: 541-346-4036 University of Oregon FAX: 541-346-5891 Eugene, OR 97403 ----------------------------------------------------------------------- ************************************************************* Q: What do dipoles say in passing? A: Have you got a moment? Q: What do dipoles say on departing? A: Debye! Q: What happens when electrons lose their energy? A: They get Bohr'ed. Q: Why do spins prefer to relax? A: Work makes them tensor. Q: How do spins communicate? A: Universal sign language. Q: How do spin students relax? A: The teacher lets them out for precess. Q: What happens when spectroscopists are idle? A: They turn from nutating nuclear spins to notating unclear puns. Q: What is the difference between 13C and 12C? A: One is magnetically resonant, the other is reticent. Q: Why are magnets so attractive? A: Have you have tried carbon-14 dating? Q: What is the Fourier transform of Bruker data? A: A varyin' baseline. Q: How are spin echos so useful? A: Many Hahn's make light work. Q: Who writes novels with a Western spin? A: Louie Larmor. Q: Why can't lawyers do NMR? A: Bar magnets have poor homogeneity. Q: Why do Republicans have trouble with NMR? A: They get plenty of polarization but little coherence. Q: Why are supercons such empty bores? A: They've no irony core. Q: Why is liquid helium so cool? A: Life ought to be a gas. Q: Why did the quantum mechanic live in the lab? A: Didn't want to commute. Q: Why do hair-dressers abhor electromagnetic fields? A: The divergence of the curl. Q: What is the result when you cross a road with a chicken? A: |road||kill|sin(theta) Q: What happens to old spectroscopists? A: They never really retire, they just change fields. Q: Why are spectroscopists never satisfied with their magnets? A: The gauss is greater on the other side of the fence. Q: Why does Old McDonald regularly switch fields? A: You get better nitrogen performance in the rotating farm. Q: Why did the quadrature detective lose his job? A: Couldn't distinguish real from imaginary. Q: What do you call old pulse sequences? A: ANACHRONYMS. Q: What was the decay constant for Julius Caesar? A: T2, Brute. Q: Why would a spectroscopist put decouple in dwell? A: One expects that Jack and Jill would remove the water. Q: Why is a NOESY like a milk shake? A: You have to use the right mixing time to get a good one. Q: Are peaks inverted in the Southern Hemisphere? A: If they are, no one is phased by them. Q: Why use a magnet for solid state NMR? A; Most facilities don't have a powder room. Q: Why don't you have to shim for solid state NMR? A: That's like asking why you don't have to flush a composting toilet. Q: Why do interferograms always evolve from the left? A: A sin is never right. (moralist) A: They don't evolve: they were created at time=0. (creationist) A: They're a dialectic between conflicting reference frames. (Marxist) Q: Why did the spectroscopist throw the clock out the window? A: To see the time-domain transform. Q: Why couldn't Prof. Fourier get a mortgage? A: No one would cosine the note. Q: Why do they say in "RMN" in French? A: I don't know, but they also get their salads in the wrong order. Q: Why do NMR spectroscopists avoid cloned sheep? A: Very baa'd cancellation in difference spectra. From owner-structural-nmr@net.bio.net Thu May 08 23:00:00 1997 Newsgroups: bionet.structural-nmr Path: biosci!daresbury!uninett.no!sn.no!nntp.uio.no!newsfeeds.sol.net!europa.clark.net!feed1.news.erols.com!news-peer.sprintlink.net!Sprint!news-pull.sprintlink.net!news.sprintlink.net!news-fw-6.sprintlink.net!rockyd!notes From: David Cowburn Subject: Light (or at least electro-magnetic ) relief X-Nntp-Posting-Host: mr0.rockefeller.edu Content-Type: text/plain; charset=us-ascii Message-ID: <3373625A.41C6@rockvax.rockefeller.edu> Sender: notes@rockyd.rockefeller.edu (News Administrator) Content-Transfer-Encoding: 7bit Organization: Rockefeller University Mime-Version: 1.0 Date: Fri, 9 May 1997 17:43:54 GMT X-Mailer: Mozilla 3.0Gold (X11; I; IRIX 5.3 IP22) Lines: 41 In order to dispel the rumor that all NMR people are totally without a trace of humor, I should like to invite the structural-nmr community to participate in a little competition. Please change the 'joke (!@#!@%!@#)' stolen from some crystallographers, listed below, to include one (or more )structural NMRists (probably best to avoid any individual). Please keep it bionet.clean, and I promise to immortalize, in some way not yet thought out, the author of the funniest (if any). " There is a molecular biologist, a crystallographer and a computer modeller in a Taxi, going for a big conference. All of a sudden, a terrorist with a big gun, jumps inside the taxi, and says: 'I will kill you all, but maybe if you do something valuable for the society and interesting I will not! So, what are you doing in your life ?' The Modeller goes: 'Well, I recently modelled an inhibitor in the active si...'BOOOOOOOM He is dead .... The Molecular Biologist goes on: 'I recently cloned a novel ras homologe protein, which seems to activate the pathway of MAP kinase and in the sequence level it has very interesting triplet repeats...' And the crystallographer interrupts: ' Excuse me, but do you mind KILLING ME NOW ?' " -- __________________________________________________________________________ David Cowburn, Box 163, The Rockefeller University, 1230 York Avenue, NY, NY 10021-6399, USA. Tel 212.327.8270. Fax 212.327.7566 http://mriris.rockefeller.edu/ Reply-To:cowburn@rockvax.rockefeller.edu From owner-structural-nmr@net.bio.net Thu May 08 23:00:00 1997 Path: biosci!BIBA.MED.TUFTS.EDU!sudha From: sudha@BIBA.MED.TUFTS.EDU ("Sudha Veeraraghavan") Newsgroups: bionet.structural-nmr Subject: Re: phosphate buffer pH 5.0? Date: 9 May 1997 08:43:17 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 22 Sender: daemon@net.bio.net Distribution: world Message-ID: <9705091135.ZM15171@biba.med.tufts.edu> NNTP-Posting-Host: net.bio.net Dear Ji Hyun Lee, We routinely use phosphate + acetate(d4) for pH near 5. --Sudha -- _______________________________________________________________________________ ******************************************************************************* !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! Sudha Veeraraghavan, Ph.D. Phone: (617) 636-6873 Department of Biochemistry MV605 Fax : (617) 636-6409 Tufts Univ. Sch. Medicine 136 Harrison Ave. E-mail: sudha@biba.med.tufts.edu Boston, MA 02111 sveerara@opal.tufts.edu !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! ******************************************************************************* _______________________________________________________________________________ From owner-structural-nmr@net.bio.net Thu May 08 23:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!csulb.edu!hammer.uoregon.edu!su-news-hub1.bbnplanet.com!news.bbnplanet.com!xfer.kren.nm.kr!snunews.snu.ac.kr!plaza.snu.ac.kr!newera From: newera@plaza.snu.ac.kr () Newsgroups: bionet.structural-nmr Subject: Mailing List for nmrpipe, nmrcapp, nmrpipp? Date: 9 May 1997 09:45:24 GMT Organization: Seoul National University, Republic of Korea Lines: 19 Message-ID: <5kurnk$276$1@snunews.snu.ac.kr> NNTP-Posting-Host: plaza.snu.ac.kr X-Newsreader: TIN [version 1.2 PL2] Hi, What address is for nmrpipe, nmrcapp, nmrpipp mailing list? Oh, happy day! Hyun -- Lee, Ji Hyun newera@plaza.snu.ac.kr Laboratory of Physical Pharmacy(Prof. Lee, Bong Jin) College of Pharmacy Seoul National University Shinlim-Dong, Kwanak-Gu Seoul 151-742, Korea. Tel : 82-2-880-7869 Fax : 82-2-872-3632 From owner-structural-nmr@net.bio.net Thu May 08 23:00:00 1997 Path: biosci!aa.wl.com!REILY From: REILY@aa.wl.com (Mike Reily 313-996-7128) Newsgroups: bionet.structural-nmr Subject: RE: phosphate buffer pH 5.0? Date: 9 May 1997 04:51:43 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 21 Sender: daemon@net.bio.net Distribution: world Message-ID: <7701520709051997.A52975.SRVR05.11B549F33B00*@MHS> NNTP-Posting-Host: net.bio.net >I wonder if it is ok to use 50mM potassium phosphate buffer _pH 5.0_ for 5mM protein sample( because it dissolves best at pH around 5 ). >To my knowlege, phosphate is suitable for pH range 6~8. My colleague says >"Although buffering capacity of phosphate buffer at pH 5.0 is small, phosphate is preferred since there is no nmr signal in case of phosphate unlike other buffer systems.". >Could you tell me your opinion? You might try deuterated acetate, which has a pKa of ~4.5. It is a viable buffer in that range. -Mike Reily Parke-Davis Pharmaceutical Research Division Warner-Lambert Company 2800 Plymouth Road Ann Arbor, Michigan 48105 313-996-7128 phone 313-998-2716 fax reily@aa.wl.com From owner-structural-nmr@net.bio.net Thu May 08 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!europa.clark.net!cpk-news-hub1.bbnplanet.com!su-news-hub1.bbnplanet.com!news.bbnplanet.com!xfer.kren.nm.kr!snunews.snu.ac.kr!plaza.snu.ac.kr!newera From: newera@plaza.snu.ac.kr () Newsgroups: bionet.structural-nmr Subject: phosphate buffer pH 5.0? Date: 9 May 1997 03:00:35 GMT Organization: Seoul National University, Republic of Korea Lines: 24 Message-ID: <5ku40j$12jm$1@snunews.snu.ac.kr> NNTP-Posting-Host: plaza.snu.ac.kr X-Newsreader: TIN [version 1.2 PL2] Hi, I wonder if it is ok to use 50mM potassium phosphate buffer _pH 5.0_ for 5mM protein sample( because it dissolves best at pH around 5 ). To my knowlege, phosphate is suitable for pH range 6~8. My colleague says "Although buffering capacity of phosphate buffer at pH 5.0 is small, phosphate is preferred since there is no nmr signal in case of phosphate unlike other buffer systems.". Could you tell me your opinion? Thanks. Lee, Ji Hyun -- email : newera@plaza.snu.ac.kr address : Lee, Ji Hyun Laboratory of Physical Pharmacy(Prof. Lee, Bong Jin) Seoul National University College of Pharmacy Shinlim-Dong, Kwanak-Gu Seoul 151-742, Korea. From owner-structural-nmr@net.bio.net Sun May 11 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!news.maxwell.syr.edu!news-was.dfn.de!news-fra1.dfn.de!news-ge.switch.ch!news-zh.switch.ch!ubaclu.unibas.ch!ubaclu.unibas.ch!nntp Newsgroups: bionet.structural-nmr Subject: Re: Light (or at least electro-magnetic ) relief Message-ID: <1997May12.101721.47498@yogi.urz.unibas.ch> From: Andrei Alexandrescu Date: 12 May 97 10:17:21 MET References: <3373625A.41C6@rockvax.rockefeller.edu> Organization: Biozentrum, U-Basel Nntp-Posting-Host: secsy.bioz.unibas.ch X-Mailer: Mozilla 1.1N (Macintosh; I; PPC) MIME-Version: 1.0 X-URL: news:3373625A.41C6@rockvax.rockefeller.edu Content-Transfer-Encoding: 7bit Content-Type: text/plain; charset=us-ascii Lines: 11 >In order to dispel the rumor that all NMR people are totally without a >trace of humor, HOHAHA HAHAHAHOHAHAHAHOHOHOHAHAHA ! Andrei From owner-structural-nmr@net.bio.net Sun May 11 23:00:00 1997 Path: biosci!OTTER.BIOCHEM.UBC.CA!mcintosh From: mcintosh@OTTER.BIOCHEM.UBC.CA (Lawrence McIntosh) Newsgroups: bionet.structural-nmr Subject: Varian conversion Date: 12 May 1997 12:47:56 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 34 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net We have recently started playing with in-line digital oversampling (dsp='i') on a Varian UNITY spectrometer. To our surprise, the fid data is now stored in floating point form, rather than integer form (which is the normal case with no in-line sampling or apparently the case with hardware oversampling, dsp='r'). The upshot is that we can no longer convert the Varian data to NMRpipe or Felix format using standard conversion programs. Has any UNITY user experienced this problem and found/written an conversion routine? Thanks! ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Lawrence McIntosh 2146 Health Sciences Mall Departments of Biochemistry and Chemistry University of British Columbia Vancouver, BC, Canada V6T 1Z3 mcintosh@otter.biochem.ubc.ca ph: (604) 822-3341 fax: (604) 822-5227 http://www.biochem.ubc.ca/ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ From owner-structural-nmr@net.bio.net Sun May 11 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!swrinde!howland.erols.net!feed1.news.erols.com!newsfeed.nacamar.de!azure.xara.net!xara.net!netcom.net.uk!nntpfeed.doc.ic.ac.uk!sunsite.doc.ic.ac.uk!yama.mcc.ac.uk!news.york.ac.uk!not-for-mail From: David Scott Newsgroups: bionet.structural-nmr Subject: 19F NMR conc Date: Mon, 12 May 1997 10:40:19 -0700 Organization: University of York. York. Y01 5DD Lines: 22 Sender: djs17@york.ac.uk Message-ID: <33775603.5E94@york.ac.uk> NNTP-Posting-Host: biolpc227.york.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Win16; I) Hia I have just purified a protein labelled with 6-fluorotryptophan (6-fltrp), does anyone out there know the extinction coefficient of 6-fltrp in a pepetide so I can work the protein concentration??? Please help! dave. xxx -- ******************************* * Dr. David Scott * * Dept Biology * * University of York * * YORK * * UK * * * * phone +44 1904 432867 * * fax +44 1904 432860 * ******************************* From owner-structural-nmr@net.bio.net Mon May 12 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!news.scripps.edu!obelix!weber From: weber@scripps.edu (Christoph Weber) Newsgroups: bionet.structural-nmr Subject: Re: phosphate buffer pH 5.0? Date: 13 May 1997 18:51:18 GMT Organization: The Scripps Research Institute Lines: 15 Distribution: world Message-ID: <5lad76$p96$1@hermes.scripps.edu> References: <5ku40j$12jm$1@snunews.snu.ac.kr> NNTP-Posting-Host: obelix.scripps.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Why using a "buffer" when potentially none is needed? If your protein does not absolutely require a buffer for stability, function or such, you can simply use water (+ azide to prevent microbial growth). At NMR concentrations, a protein buffers itself quite well, certainly much better than phosphate could at pH 5.0. My 2cts, Christoph -- | Dr. Christoph Weber Sen. Research Associate | Dept.of Molecular Biology, MB9 619-784-9869 (phone) | The Scripps Research Institute 619-784-9985 (FAX) | La Jolla CA 92037-1027 weber@scripps.edu | http://www.scripps.edu/~chazin/people/cw.html From owner-structural-nmr@net.bio.net Mon May 12 23:00:00 1997 Newsgroups: bionet.molec-model,bionet.structural-nmr,bionet.xtallography Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!news.maxwell.syr.edu!azure.xara.net!xara.net!netcom.net.uk!nntpfeed.doc.ic.ac.uk!sunsite.doc.ic.ac.uk!lyra.csx.cam.ac.uk!hgmp.mrc.ac.uk!ebi.ac.uk!news From: David Starks-Browning Subject: Parallel Programming Course at EMBL-Heidelberg Message-ID: Sender: starksb@sol19.ebi.ac.uk Date: Mon, 12 May 1997 21:07:21 GMT Lines: 58 Organization: EBI - European Bioinformatics Institute X-Newsreader: Gnus v5.3/Emacs 19.34 Xref: biosci bionet.molec-model:1546 bionet.structural-nmr:1944 bionet.xtallography:3428 [please post and distribute] The Supercomputing Resource for Molecular Biology (SRMB), located at the European Molecular Biology Laboratory in Heidelberg, is offering training courses in Parallel Programming and High Performance Computing to European researchers in molecular biology. The next course will be held 8 - 14 June 1997. The course is an intensive and comprehensive introduction to parallel programming and high performance computing for European molecular biologists. Topics include: Heterogeneous distributed computing with PVM Message passing programming with MPI Data parallel programming with Fortran90/HPF Methodologies for designing parallel algorithms Performance modeling and scalability analysis Optimization for modern microprocessors Shared memory programming and parallelizing compilers Case studies from parallel applications in molecular biology There is considerable emphasis on hands-on experience through practical sessions. In particular, participants will write and execute their own parallel programs in PVM, MPI and Fortran90/HPF. The course is open to European researchers at the advanced post-graduate level who have research interests in molecular biology, including sequence analysis, image processing, structure refinement, protein design and molecular dynamics. Participants from EU countries will be supported by an EU HCM/ALSF grant. Residents of other EMBL member countries will be supported by funds from EMBL. Participants receive travel, accommodation and a subsistence allowance during their visit, as well as access to the computational facilities of the Laboratory. If you wish to attend you should fill out an online application form AS SOON AS POSSIBLE. Further information, with links to a detailed agenda and application forms, can be found at: ========================================================================= David Starks-Browning | starksb@ebi.ac.uk Technology Transfer Node Manager ! European Bioinformatics Institute ! Wellcome Trust Genome Campus ! tel: +44(0)1223-49 46 16 Hinxton, Cambridge CB10 1SD, UK ! fax: +44(0)1223-49 44 68 ========================================================================= From owner-structural-nmr@net.bio.net Tue May 13 23:00:00 1997 Path: biosci!ALCHEMIST.CHEM.MQ.EDU.AU!PKARUSO From: PKARUSO@ALCHEMIST.CHEM.MQ.EDU.AU ("peter karuso") Newsgroups: bionet.structural-nmr Subject: Postdoc/PhD positions Date: 13 May 1997 20:02:08 -0700 Organization: School of Chemistry, Macquarie Uni Lines: 49 Sender: daemon@net.bio.net Distribution: world Message-ID: <20B4B267523@alchemist.chem.mq.edu.au> Reply-To: pkaruso@alchemist.chem.mq.edu.au NNTP-Posting-Host: net.bio.net The new Chemistry building is coming along and should be opened in October. Our new 600 and a DMX upgrade of our 400 will arrive at about the same time. So then we will have 2 x 600 (one at another University; UNSW) and 1 x 400 MHz NMR spectrometers and ready to GO!! However, we need students and post-docs. There is the possibity for funding for PhD (4 scholarships, ~ $15,000/annum + tuition fees (OPRS; ~$15,000) for 3 - 4 years) and post-docs (4 fellowships, ~$45,000/annum for 2 years) at Macquarie University. These are open to any nationality but competition is tough. In addition, I would be happy to sponsor anyone who would like to apply for a fellowship from their own country, again, I could write up a suitable project. I have two projects. One on the the NMR structure elucidation of Ru metal complexes bound (groove binders and intercalators) to DNA (oligos) and the other on the NMR structure elucidation of mimics of FK506 bound to FKBP. I can write a substantial amount of the application if anyone is interested. The post-doc applications are due middle of June and the application forms and guidelines can be obtained over the internet: http://www.ro.mq.edu.au/MURF/murfguid.htm PhD scholarship applications (OPRS and MUPRA) are not due till October 31st so the forms are not yet available but if anyone is interested then please let me know (e-mail) so that a project can be planned and applications forwarded at the earliest time. Also please visit our web site http://www.chem.mq.edu.au/academics/karuso.htm Other areas of mutual interest may also be suitable projects for the above opportunities. Peter ********************************************************************** Dr. Peter Karuso _--_|\ Senior Lecturer Email: peter.karuso@mq.edu.au / CHEM \ School of Chemistry Voice: +61 2 9850-8290 (Office) \_.--._* Macquarie University +61 2 9850-8305 (Lab) * v NSW 2109 Australia FAX: +61-2-9850-8313 * * http://www.chem.mq.edu.au/academics/karuso.htm . http://www.chem.mq.edu.au/~dmoran/grphome.html * ********************************************************************** From owner-structural-nmr@net.bio.net Tue May 13 23:00:00 1997 Path: biosci!PHOENIX.PRINCETON.EDU!ipelczer From: ipelczer@PHOENIX.PRINCETON.EDU (Istvan Pelczer) Newsgroups: bionet.structural-nmr Subject: RNA/DNA nmr Date: 14 May 1997 05:45:12 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 27 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net To all, who could be interested, We have a review published recently which might be of your interest: P. N. Borer, L. Pappalardo, D. J. Kerwood, and I. Pelczer; "NMR-based structure determination for unlabeled RNA and DNA" in: Advances in Biophysical Chemistry, Vol. 6., 1997 (Ed.: C. A. Bush) JAI Press, Inc., Greenwich, CT/USA and London/UK, pp 173-216 Take a look. This is not a (paid) commercial... :) Istvan wwww,wwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwww Istvan Pelczer, Ph.D. Email: ipelczer@princeton.edu Senior NMR Spectroscopist Princeton University Department of Chemistry, Frick Lab., ph# (609) 258 2342 Washington Road fax# (609) 258 6746 Princeton, NJ 08544, USA From owner-structural-nmr@net.bio.net Tue May 13 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!europa.clark.net!worldnet.att.net!news.u.washington.edu!protein.chem.washington.edu!tomasz From: John Tomaszewski Newsgroups: bionet.structural-nmr,bionet.molbio.proteins Subject: Universal 13C-Asp labelling of Protein Date: Tue, 13 May 1997 18:05:13 -0700 Organization: University of Washington Lines: 31 Message-ID: References: <01bc5ff6$94bc6760$7533f8ce@scottm> NNTP-Posting-Host: protein.chem.washington.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII In-Reply-To: <01bc5ff6$94bc6760$7533f8ce@scottm> Xref: biosci bionet.structural-nmr:1946 bionet.molbio.proteins:10733 We have overexpressed a small (15KDa) protein and a couple of mutants in both LB and minimal media (for 15N labelling) in our lab but now we have a need to express it (and one mutant) universally 13C-Asp labelled. What I'm trying to hear are peoples experiences with some of the options along these lines. It seems to me that it might be possible with either LB or minimal media seeing as how ~25% 13C enrichment should be plenty for 13C NMR. Some of my questions are: 1) Can we simply spike LB with 13C-Asp and achieve the desired level of enrichment or will the 13C-Asp be broken down and the 13C scattered around too fast? 2) Would minimal media with all AAs (and only 13C-Asp) supplied be a better way to go? 3) What is the metabolic fate of the 13C in 13C-Asp? 4) Would be worth our effort to transfer the plasmid to the appropriate auxotroph and go from there? Any help, suggestions, pointers to papers, etc. would be greatly appreciated. Thank you. Sincerely, John T. ******************************************************************** ** ** ** ** * ** DEPARTMENT OF CHEMISTRY ** ** ** ** ** * ** tomasz@protein.chem.washington.edu ** ** ** ** ** * ** (206) 616-2993 ** ** ***** ***** JOHN TOMASZEWSKI ** ******************************************************************** From owner-structural-nmr@net.bio.net Wed May 14 23:00:00 1997 Path: biosci!MDH.BIM.ANL.GOV!zfeng From: zfeng@MDH.BIM.ANL.GOV (Zhou Feng) Newsgroups: bionet.structural-nmr Subject: (none) Date: 15 May 1997 08:12:03 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <199705151512.KAA06853@pyp.bim.anl.gov> NNTP-Posting-Host: net.bio.net UNsubscribde feng@lisboa.ks.uiuc.edu From owner-structural-nmr@net.bio.net Wed May 14 23:00:00 1997 Path: biosci!MDH.BIM.ANL.GOV!zfeng From: zfeng@MDH.BIM.ANL.GOV (Zhou Feng) Newsgroups: bionet.structural-nmr Subject: (none) Date: 15 May 1997 08:12:20 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <199705151512.KAA06857@pyp.bim.anl.gov> NNTP-Posting-Host: net.bio.net unsubscribe zfeng@enzyme.bim.anl.gov From owner-structural-nmr@net.bio.net Wed May 14 23:00:00 1997 Path: biosci!MDH.BIM.ANL.GOV!zfeng From: zfeng@MDH.BIM.ANL.GOV (Zhou Feng) Newsgroups: bionet.structural-nmr Subject: (none) Date: 15 May 1997 08:12:02 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <199705151512.KAA06855@pyp.bim.anl.gov> NNTP-Posting-Host: net.bio.net unsubscribe feng@anl.gov From owner-structural-nmr@net.bio.net Thu May 15 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!europa.clark.net!newsfeed.internetmci.com!news1.best.com!nntp2.ba.best.com!slp.vip.best.com!user From: slp@stevenscreek.com (Steve Patt) Newsgroups: bionet.structural-nmr Subject: PR: HP Plotter Emulator for the Mac - New Release Date: 16 May 1997 16:45:11 GMT Organization: Stevens Creek Software/The Athlete's Bookstore Lines: 48 Message-ID: NNTP-Posting-Host: slp.vip.best.com PLOTVIEW 4.0 RELEASED STEVENS CREEK SOFTWARE UPDATES ITS HP PLOTTER EMULATOR FOR THE MACINTOSH CUPERTINO, Calif.--May 16, 1997--Stevens Creek Software announced today that it has begun shipping the long-awaited update to its first commercial product, PlotView (tm) for the Macintosh. Since its introduction in 1988, PlotView has been the only Hewlett-Packard plotter emulator on the market, not only translating files of Hewlett-Packard Graphics Language (HP-GL) commands into PICT images, but also accepting HP-GL input direct via the serial port of the Macintosh. Users of PlotView include users of a wide variety of scientific instruments, digital oscilloscopes, and computer software on other platforms which produces HP-GL output. PlotView 4.0 is a 32-bit clean version that runs on all PowerMacs and is compatible with the latest System software. It also features: o Greater flexibility and reliability when connecting to scientific instruments or other computers via the serial port, giving users control over handshaking options and port selection. o Increased control over the HP-GL interpretation, giving users more choices in line thickness and a wider range of font sizes. o Significantly higher resolution printed output than earlier versions. Version 4.0 of PlotView is shipping now. Full information about the software, sample "plots" illustrating a wide range of applications, as well as a complete online HTML manual, can be found at: . A functional trial copy of the software (with limitations on printing and saving) can also be downloaded from the site. Users of older versions of the software can order upgrades at the Web site, or by email (sales@stevenscreek.com), phone (1-800-823-4279) or fax (1-408-725-0424). ############################################ PlotView is a registered trademark of Stevens Creek Software. All other trademarks are property of their respective owners. Electronic screen shots and review copies of PlotView are available upon request. Inquiries regarding distribution of PlotView are welcome; reseller prices are available. For more information contact: Steven Patt President, Stevens Creek Software Phone: (408-725-0424) E-mail: plotview@stevenscreek.com From owner-structural-nmr@net.bio.net Fri May 16 23:00:00 1997 Path: biosci!OTTER.BIOCHEM.UBC.CA!mcintosh From: mcintosh@OTTER.BIOCHEM.UBC.CA (Lawrence McIntosh) Newsgroups: bionet.structural-nmr Subject: Varian Conversion Date: 16 May 1997 20:19:27 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 38 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Thanks to those who replied to my question regarding conversion of Varian data recorded with in-line digital oversampling (dsp='i'). In contrast to no oversampling or hardware oversampling (dsp='r'), which yield interger data, the software oversampling produces floating point data. In the case of nmrPipe, the var2pipe command with -dsp takes care of the floating point data. In the case of Felix, the Varian userlib has a modified version of the program varian2felix that handles both integer and floating point data. Finally, for gradient data recorded in sensitivity-enhanced mode, David Horita in Andy Byrd's lab kindly provided a modified version of Lewis Kay's grad_sort program. Cheers, ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Lawrence McIntosh 2146 Health Sciences Mall Departments of Biochemistry and Chemistry University of British Columbia Vancouver, BC, Canada V6T 1Z3 mcintosh@otter.biochem.ubc.ca ph: (604) 822-3341 fax: (604) 822-5227 http://www.biochem.ubc.ca/ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ From owner-structural-nmr@net.bio.net Fri May 16 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!howland.erols.net!newsfeed.internetmci.com!in3.uu.net!196.25.1.18!news1.saix.net!usenet From: "M.Shackleford" Newsgroups: bionet.structural-nmr Subject: Panis (eyes) Date: Sat, 17 May 1997 19:45:02 -0700 Organization: The South African Internet Exchange. Lines: 32 Message-ID: <337E6D2E.5BC4@cis.co.za> NNTP-Posting-Host: net-53-099.cis.co.za Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Win95; I; 16bit) ADVICE NEEDED ON PANIS My dog Lucy, a 9 year old alsation, has an eye infliction known as PANIS. She was as good as blind last year when I had her operated on. For two weeks after the operation,she had blood blisters on the eye balls.When this cleared up I noticed that the smoke was still there. The black skin started growing back almost immediatly and a year later I am back to square one. Since she was diagnosed of it two years ago, I have been using 'PRED FORTE' eyedrops about three times a day. Whether this is actualy helping to retard the growth, I can't say, I haven't stopped the drops long enough to find out. I don't want to put her through the trauma of another operation unnesseceraly and putting her to sleep is definitly NOT even a last resort. I would appreciate ANY constructive comment,thought,alternative medication, tip,anything at all,that I could discuss with my Vet. Anybody with any advice,reguardless of how trivia you may consider it, PLEASE let me know, it may lead to the answers I so desperately need. Leave your reply on this newsgroup or drop me an email at shcklfrd@cis.co.za Thank you Mike From owner-structural-nmr@net.bio.net Fri May 16 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!swrinde!rill.news.pipex.net!pipex!newsfeed.nacamar.de!blackbush.xlink.net!news-ge.switch.ch!serra.unipi.it!sirio.cineca.it!carbon.foodsci.unibo.it!mauro From: "Mauro A. Cremonini" Newsgroups: bionet.structural-nmr Subject: Aspect 3000 parallel port slow?? Date: Sun, 18 May 1997 13:06:46 +0200 Organization: Cineca Lines: 30 Message-ID: Reply-To: "Mauro A. Cremonini" NNTP-Posting-Host: carbon.foodsci.unibo.it Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Hi! I am writing a program for tranferring data from the parallel port of the Bruker aspect 3000 computer to the parallel port of a PC. I know that at least 2 program exist for doing it, but I decided to do it myself as a challenge :-). I wrote a pascal program for the aspect and a machine code program for the pc, but with my great surprize the maximum tranfer rate I got was 1.6 Kbyte / sec, differently from what is reported in the literature (by 2 groups: the one that wrote INTERLINK and the one that wrote ZZNET). In fact it is said that rates as high as 33Kbytes/sec can be easily achieved. The problem seems to be the Aspect computer that, as far as I can see, does not send data through the parallel port with a rate faster that 1.6 Kbyte/sec. Has anybody experience of such a problem? Can anybody give me a hint for making the sistem faster? Thank you very much for your help. Mauro ################# PLEASE NOTE CHANGE OF ADDRESS ############# ============================================ Mauro Andrea Cremonini, PhD Food Science and Technology Laboratory University of Bologna Via Ravennate 1020 - Cesena - Italy FAX:+39.547.382348 e-mail: mauro@carbon.foodsci.unibo.it 73 de IK4QIX ============================================ From owner-structural-nmr@net.bio.net Sun May 18 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!europa.clark.net!newsxfer3.itd.umich.edu!newsxfer.itd.umich.edu!news.mtu.edu!msunews!netnews.upenn.edu![130.91.171.101]!chris From: chris@highresnmr.biophys.upenn.edu (Krzysztof Wroblewski) Newsgroups: bionet.structural-nmr Subject: Re: Aspect 3000 parallel port slow?? Date: Mon, 19 May 1997 17:31:21 GMT Organization: University of Pennsylvania Lines: 26 Message-ID: References: NNTP-Posting-Host: nmprc2.biophys.upenn.edu X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] In article "Mauro A. Cremonini" writes: >From: "Mauro A. Cremonini" >Subject: Aspect 3000 parallel port slow?? >Date: Sun, 18 May 1997 13:06:46 +0200 >Hi! I am writing a program for tranferring data from the parallel port of >the Bruker aspect 3000 computer to the parallel port of a PC. I know that >at least 2 program exist for doing it, but I decided to do it myself as a >challenge :-). >I wrote a pascal program for the aspect and a machine code >program for the pc, but with my great surprize the maximum tranfer rate I >got was 1.6 Kbyte / sec, differently from what is reported in the >literature (by 2 groups: the one that wrote INTERLINK and the one that >wrote ZZNET). >In fact it is said that rates as high as 33Kbytes/sec can be >easily achieved. The problem seems to be the Aspect computer that, as far >as I can see, does not send data through the parallel port with a rate >faster that 1.6 Kbyte/sec. Has anybody experience of such a problem? Can >anybody give me a hint for making the sistem faster? >Thank you very much for your help. I am afraid that the bottleneck is the Pascal program. Try to write Aspect 3000 part in the Assembler. Krzysztof Wroblewski From owner-structural-nmr@net.bio.net Mon May 19 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!howland.erols.net!surfnet.nl!ruu.nl!not-for-mail From: "Bas R. Leeflang" Newsgroups: bionet.structural-nmr Subject: Position available at Utrecht University Date: Tue, 20 May 1997 12:17:56 +0200 Organization: BOC Lines: 56 Message-ID: <33817A54.4243B223@boc.chem.ruu.nl> NNTP-Posting-Host: bocpc1.chem.ruu.nl Mime-Version: 1.0 Content-Type: multipart/mixed; boundary="------------410C62752D6C60A92AE6DB84" X-Mailer: Mozilla 3.0 (X11; I; Linux 2.0.27 i586) This is a multi-part message in MIME format. --------------410C62752D6C60A92AE6DB84 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit -- Bio-Organic Chemistry group Dr. Bas R. Leeflang Bijvoet Center for Biomolecuar Research Tel. : int+31.30.253.3498 Utrecht University Fax : int+31.30.254.0980 Padualaan 8, NL-3584 CH Utrecht Email: b.r.leeflang@chem.ruu.nl The Netherlands http://www.boc.chem.ruu.nl --------------410C62752D6C60A92AE6DB84 Content-Type: text/plain; charset=us-ascii; name="NCAM.vac" Content-Transfer-Encoding: 7bit Content-Disposition: inline; filename="NCAM.vac" Vacancies at the Bijvoet Center for BioMolecular Research, dept. of Bio-Organic Chemistry The research program of the carbohydrate section of the Department of Bio-Organic Chemistry, Bijvoet Center for Biomolecular Research at Utrecht University, The Netherlands (Prof. Dr. J.F.G. Vliegenthart, Prof. Dr. J.P Kamerling), is focused on primary/spatial structure analysis of glycoproteins, glycan receptors, glycoprotein glycans and polysaccharides, biosynthesis of oligosaccharide sequences, and organic synthesis of oligosaccharides. The Board of the department likes to announce the following vacancie, which is immediately available: A PhD student position (4 years) for a topic on the structural and functional involvement of oligomannose-type glycoprotein N-glycans in the interaction between neural recognition molecules. In this research the candidate will study the three-dimensional structure of complexes of the oligomannose-type glycans with the NCAM 1 Ig4 and of the isolated Ig4, using advanced NMR techniques and theoretical approaches. The project may include isotope labeling of the proteins. At the end of the term a thesis leading to a doctorate at Utrecht University has to be prepared. Only nationals of a European country are invited to reflect. Experience with either handling proteins, NMR spectroscopy or conformational analysis is required. If you are interested and have the right academic qualification for starting in a PhD student position in the Netherlands, please send your letter and a CV to: Prof. Dr. J.F.G. Vliegenthart, Bijvoet Center, Utrecht University, P.O. box 80.075, NL-3508 TB Utrecht The Netherlands --------------410C62752D6C60A92AE6DB84-- From owner-structural-nmr@net.bio.net Mon May 19 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!europa.clark.net!news-peer.sprintlink.net!news.sprintlink.net!Sprint!newsxfer3.itd.umich.edu!newsxfer.itd.umich.edu!news.mtu.edu!msunews!netnews.upenn.edu![130.91.171.101]!chris From: chris@highresnmr.biophys.upenn.edu (Krzysztof Wroblewski) Newsgroups: bionet.structural-nmr Subject: Re: Upset Neighbors Date: Tue, 20 May 1997 19:37:19 GMT Organization: University of Pennsylvania Lines: 13 Distribution: world Message-ID: References: <2.2.32.19970520131908.00675160@mailhost.tcs.tulane.edu> NNTP-Posting-Host: nmprc2.biophys.upenn.edu X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] In article <2.2.32.19970520131908.00675160@mailhost.tcs.tulane.edu> lyttle@MAILHOST.TCS.TULANE.EDU (Tom Lyttle) writes: >From: lyttle@MAILHOST.TCS.TULANE.EDU (Tom Lyttle) >Subject: Upset Neighbors >Date: 20 May 1997 06:17:35 -0700 >Hi Folks, > You were recommended to me by someone on the Magnetic Resonance Lab >Managers net as some who could possibly help provide an answer to my problem >listed below. Thanks for any advise. Tom Contact Magnex Scientific (510)229-8000 Krzysztof Wroblewski From owner-structural-nmr@net.bio.net Mon May 19 23:00:00 1997 Path: biosci!cnrs-orleans.fr!sodano From: sodano@cnrs-orleans.fr (Sodano Patrick) Newsgroups: bionet.structural-nmr Subject: (none) Date: 20 May 1997 12:19:53 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: <199705201919.VAA08673@admin.cnrs-orleans.fr> NNTP-Posting-Host: net.bio.net unsubscribe ************************************************************************* Dr Sodano Patrick Centre de Biophysique Mol=E9culaire Rue Charles Sadron 45071 Orl=E9ans C=E9dex 02 France ************************************************************************ From owner-structural-nmr@net.bio.net Mon May 19 23:00:00 1997 Path: biosci!UIC.EDU!shantha From: shantha@UIC.EDU (Shantha Samarasinghe) Newsgroups: bionet.structural-nmr Subject: Re: Upset Neighbors Date: 20 May 1997 09:24:57 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 83 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <2.2.32.19970520131908.00675160@mailhost.tcs.tulane.edu> NNTP-Posting-Host: net.bio.net You can install the shield on the ceiling underneath the magnet without moving it. Since the ceiling can be accessed from the floor below you this should not be a problem. Only make sure when you move magnetic objects in the lower floor, that you are doing it slowly without risking quenching the magnet above. ------------------------------------------------------------ Shantha D. Samarasinghe 3001 S. King Drive #514 Chicago, IL 60616 e-mail: shantha@uic.edu ------------------------------------------------------------ On 20 May 1997, Tom Lyttle wrote: > Hi Folks, > You were recommended to me by someone on the Magnetic Resonance Lab > Managers net as some who could possibly help provide an answer to my problem > listed below. Thanks for any advise. Tom > > >Date: Mon, 19 May 1997 14:55:28 -0500 > >To: ammrl@bloch.cchem.berkeley.edu > >From: Tom Lyttle > >Subject: Upset Neighbors > >Cc: Bill, Debby > >Bcc: rthart@mailhost.tcs.tulane.edu > > > >Dear AMMRL Experts! > > Our 500 MHz NMR is located on the sixth floor of our building. The > fifth floor contains Biomedical Engineers who, admittedly, do not understand > much about the difference between RF electromagnetic fields (EMF) and static > magnetic fields. They have given the lab directly below the magnet to a new > researcher (coming in at the assistant prof level) who will be doing a lot > of cell cultures. The axial stray field in that room (from our magnet) > ranges from 6 gauss at the ceiling to 1 gauss at the floor and an > >"ambient" field of 4 gauss. These measurements were made by our OEHS person > using a "Annis Model 25 Magnetometer". > > > > My problem is that the new person is insisting that she has checked > with her colleagues in her field and that that kind of a stray field will > mess up her experiments. They want me to fix the problem by moving the > magnet (not economically feasible) or by placing anywhere from 1 to 3 4'X8' > 1/2 inch thick steel plates under the magnet. I told them that could be very > expensive with powering down the magnet and re-energizing with new cryogens, > shimming, etc. (At first they wanted a Faraday cage - they do not seem to > understand that will not help.) The obvious answer is to move her lab, but > they are not yet ready to accommodate that remedy. > > > > Do you know where I can get any expert advise to "quench" their > concerns? Do you know of anyone who has had problems with cell cultures in > static magnetic fields? Any help will be greatly appreciated. Thanks. > > > > > >Tom Lyttle > >Director, Coordinated Instrumentation Facility > >Tulane University > >604 Lindy Boggs > >New Orleans, LA 70118-5698 > > > >PH: (504) 865-5142 > >FAX:(504) 865-6768 > >E-MAIL: lyttle@mailhost.tcs.tulane.edu > > > >http://www.tulane.edu/~cif > > > > > > > > > Tom Lyttle > Director, Coordinated Instrumentation Facility > Tulane University > 604 Lindy Boggs > New Orleans, LA 70118-5698 > > PH: (504) 865-5142 > FAX:(504) 865-6768 > E-MAIL: lyttle@mailhost.tcs.tulane.edu > > http://www.tulane.edu/~cif > > From owner-structural-nmr@net.bio.net Mon May 19 23:00:00 1997 Path: biosci!MAILHOST.TCS.TULANE.EDU!lyttle From: lyttle@MAILHOST.TCS.TULANE.EDU (Tom Lyttle) Newsgroups: bionet.structural-nmr Subject: Upset Neighbors Date: 20 May 1997 06:17:35 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 66 Sender: daemon@net.bio.net Distribution: world Message-ID: <2.2.32.19970520131908.00675160@mailhost.tcs.tulane.edu> NNTP-Posting-Host: net.bio.net Hi Folks, You were recommended to me by someone on the Magnetic Resonance Lab Managers net as some who could possibly help provide an answer to my problem listed below. Thanks for any advise. Tom >Date: Mon, 19 May 1997 14:55:28 -0500 >To: ammrl@bloch.cchem.berkeley.edu >From: Tom Lyttle >Subject: Upset Neighbors >Cc: Bill, Debby >Bcc: rthart@mailhost.tcs.tulane.edu > >Dear AMMRL Experts! > Our 500 MHz NMR is located on the sixth floor of our building. The fifth floor contains Biomedical Engineers who, admittedly, do not understand much about the difference between RF electromagnetic fields (EMF) and static magnetic fields. They have given the lab directly below the magnet to a new researcher (coming in at the assistant prof level) who will be doing a lot of cell cultures. The axial stray field in that room (from our magnet) ranges from 6 gauss at the ceiling to 1 gauss at the floor and an >"ambient" field of 4 gauss. These measurements were made by our OEHS person using a "Annis Model 25 Magnetometer". > > My problem is that the new person is insisting that she has checked with her colleagues in her field and that that kind of a stray field will mess up her experiments. They want me to fix the problem by moving the magnet (not economically feasible) or by placing anywhere from 1 to 3 4'X8' 1/2 inch thick steel plates under the magnet. I told them that could be very expensive with powering down the magnet and re-energizing with new cryogens, shimming, etc. (At first they wanted a Faraday cage - they do not seem to understand that will not help.) The obvious answer is to move her lab, but they are not yet ready to accommodate that remedy. > > Do you know where I can get any expert advise to "quench" their concerns? Do you know of anyone who has had problems with cell cultures in static magnetic fields? Any help will be greatly appreciated. Thanks. > > >Tom Lyttle >Director, Coordinated Instrumentation Facility >Tulane University >604 Lindy Boggs >New Orleans, LA 70118-5698 > >PH: (504) 865-5142 >FAX:(504) 865-6768 >E-MAIL: lyttle@mailhost.tcs.tulane.edu > >http://www.tulane.edu/~cif > > > Tom Lyttle Director, Coordinated Instrumentation Facility Tulane University 604 Lindy Boggs New Orleans, LA 70118-5698 PH: (504) 865-5142 FAX:(504) 865-6768 E-MAIL: lyttle@mailhost.tcs.tulane.edu http://www.tulane.edu/~cif From owner-structural-nmr@net.bio.net Wed May 21 23:00:00 1997 Path: biosci!daresbury!uninett.no!Norway.EU.net!EU.net!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!feed1.news.erols.com!disgorge.news.demon.net!demon!dispatch.news.demon.net!demon!uknet!uknet!uknet!newsfeed.ed.ac.uk!news From: Alice Soteriou Newsgroups: bionet.structural-nmr Subject: PhD Opportunities 1997 Date: Wed, 21 May 1997 16:42:47 +0100 Organization: Edinburgh University Message-ID: <338317F7.3BB8@chem.ed.ac.uk> NNTP-Posting-Host: wadham.chem.ed.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0Gold (X11; I; SunOS 5.4 sun4m) Lines: 40 Postgraduate Opportunities 1997 -------------------------------- A PhD place is available in the Edinburgh Biomolecular NMR Unit starting on October 1 1997. The project will be concerned with examples of viral proteins that are pirated copies of human proteins. The viral versions serve to defend the virus against attack by the human complement and immune systems. They have potential value as anti-inflammatories and in xenotransplantion. The project will entail detailed structural and functional studies of viral mimics and will utilise the following techniques: recombinant protein expression, protein purification, protein characterisation (mass spectrometry etc.), data collection using our 1995 Varian INOVA 600 MHz spectrometer, spectral assignment and structure calculation on a suite of Silicon Graphics workstations. Sequence database searching, computer assisted sequence alignment and molecular modelling will also be used. Training in all of these skills will be provided. Deadline: ---------- Contact as soon as possible Contact Details: ---------------- Dr Paul Barlow University of Edinburgh Department of Chemistry Joseph Black Building West Mains Road Edinburgh EH9 3JJ Tel: +44 (0) 131 650 4727 Fax: +44 (0) 131 650 4743 email: barlow@chem.ed.ac.uk http://www.chem.ed.ac.uk/bionmr/bionmr.html From owner-structural-nmr@net.bio.net Wed May 21 23:00:00 1997 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.structural-nmr Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 22 May 1997 02:00:14 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Distribution: world Message-ID: <199705220900.CAA08582@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-structural-nmr@net.bio.net Thu May 22 23:00:00 1997 Newsgroups: bionet.structural-nmr Path: biosci!daresbury!uninett.no!sn.no!nntp.uio.no!newsfeeds.sol.net!europa.clark.net!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!howland.erols.net!rill.news.pipex.net!pipex!server1.netnews.ja.net!server2.netnews.ja.net!bradford.ac.uk!leeds.ac.uk!news From: genjrpa@leeds.ac.uk (John Arnold) Subject: Re: Upset Neighbors Message-ID: <1997May23.120405.21701@leeds.ac.uk> NNTP-Posting-Host: chmibm53.leeds.ac.uk X-Mailer: Mozilla 1.2N (Windows; I; 16bit) Content-Type: text/plain; charset=us-ascii Organization: University of Leeds MIME-Version: 1.0 Date: Fri, 23 May 1997 13:04:05 +0100 (BST) References: <2.2.32.19970520131908.00675160@mailhost.tcs.tulane.edu> Lines: 13 Content-Transfer-Encoding: 7bit Are the cell cultures being grown in incubators? If so, they may have a glass window or two but otherwise consist of a housing which is ferromagnetic, or whatever the correct term is . Then a measurement of the stray field within an incubator could show that there is no problem. I don't know how practical such a measurement would be to perform with the proper equipment, so perhaps just a compass could be used instead. This could confirm the absence of stray field within any unit, if you can see the compass needle through a window! John Arnold Dept. of Biology Univ. of Leeds, UK From owner-structural-nmr@net.bio.net Thu May 22 23:00:00 1997 Path: biosci!beri.co.jp!hiroakih From: hiroakih@beri.co.jp (=?ISO-2022-JP?B?GyRCVyJMQCEhPSgwbBsoQg==?=) Newsgroups: bionet.structural-nmr Subject: Complete lists for M9 broth (for NMR) ? Date: 23 May 1997 02:39:27 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 22 Sender: daemon@net.bio.net Distribution: world Message-ID: <199705230938.SAA24349@ns.beri.co.jp> NNTP-Posting-Host: net.bio.net Dear Colleagues; I have a special interest about some "spices" for M9 minimal medium, in order to maximize yield of labeled protein. Now I'm using the simplest one (Ca2+, Mg2+ and thiamin). Then, I suppose there may be a lot of variation of some additives, i.e. nucleoside & nucleotide, biotin, Mn2+ and Fe3+, sometime Zn2+, and so on. But which one is important and which is not ? Because of a financial reason, I like to double the yield of the labeled protein from M9 medium. If you anyone know "the best recommended" M9 recipe, please let me know. Also, I like to know which one ingredient is most effective. Thank you for your attention. ---------------------------------- Hidekazu HIROAKI, research scientist, NMRG, Dept of Str. Biology, BERI (Biomol Eng Research. Inst) OSAKA ---------------------------------- From owner-structural-nmr@net.bio.net Thu May 22 23:00:00 1997 Newsgroups: bionet.structural-nmr Path: biosci!rutgers.rutgers.edu!newsin.iconnet.net!news.inc.net!uwm.edu!news.he.net!newsfeed.direct.ca!europa.clark.net!news-peer.sprintlink.net!news.sprintlink.net!Sprint!howland.erols.net!rill.news.pipex.net!pipex!server1.netnews.ja.net!server2.netnews.ja.net!bradford.ac.uk!leeds.ac.uk!news From: genjrpa@leeds.ac.uk (John Arnold) Subject: Re: Upset Neighbors Message-ID: <1997May23.121406.24507@leeds.ac.uk> NNTP-Posting-Host: chmibm53.leeds.ac.uk X-Mailer: Mozilla 1.2N (Windows; I; 16bit) Content-Type: text/plain; charset=us-ascii Organization: University of Leeds MIME-Version: 1.0 Date: Fri, 23 May 1997 13:14:06 +0100 (BST) References: <2.2.32.19970520131908.00675160@mailhost.tcs.tulane.edu> <1997May23.120405.21701@leeds.ac.uk> Lines: 9 Content-Transfer-Encoding: 7bit Er, to add to my previous posting, I forgot that this will only be valid if the incubator itself doesn't have a motor or anything which will generate a magnetic field, static or otherwise, but then the cells would presumably notice anyway (or not). John Arnold Dept of Biology Univ of Leeds, UK From owner-structural-nmr@net.bio.net Thu May 22 23:00:00 1997 Path: biosci!S151H17.REGA.KULEUVEN.AC.BE!eveline From: eveline@S151H17.REGA.KULEUVEN.AC.BE (Eveline Lescrinier) Newsgroups: bionet.structural-nmr Subject: Biosym/NMRrefine: noesy back-calculation Date: 23 May 1997 08:11:20 -0700 Organization: REGA-Institute, KULeuven Lines: 18 Sender: daemon@net.bio.net Distribution: world Message-ID: <3385B304.41C6@s151h17.rega.kuleuven.ac.be> NNTP-Posting-Host: net.bio.net I'm doing a PhD in Leuven on nmr of nucleotides. I tried to do NOE back-calculation using the Biosym NMRrefine module, which offers relaxation matrix calculations (IRMA). I have the impression that at in theoretical spectra with longer mixing times (ex: 200ms) the indirect noesy crosspeaks are to weak compared to the experimental data. Is there any person on the net who has a suggestion what I could have done wrong?? -- Eveline Lescrinier Laboratory of Medicinal Chemistry Rega Institute of Medical Research Katholieke Universiteit Leuven Minderbroedersstraat 10, B-3000 Leuven, Belgium E-mail: eveline@s151h17.rega.kuleuven.ac.be -- From owner-structural-nmr@net.bio.net Tue May 27 23:00:00 1997 Path: biosci!mmm.com!bcerickson From: bcerickson@mmm.com Newsgroups: bionet.structural-nmr Subject: Advertisements Date: 28 May 1997 13:51:59 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I thought we got a note a couple of months ago that this newsgroup would soon be "moderated"--i.e. only one person would have to read all the junk-email and would weed it out before posting legitimate messages to the group. Does anyone know what happened to that? From owner-structural-nmr@net.bio.net Tue May 27 23:00:00 1997 Path: biosci!ukc.ac.uk!M.J.Howard From: M.J.Howard@ukc.ac.uk ("M.J.Howard") Newsgroups: bionet.structural-nmr Subject: Advertisments Date: 28 May 1997 02:54:57 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 36 Sender: daemon@net.bio.net Distribution: world Message-ID: Reply-To: "M.J.Howard" NNTP-Posting-Host: net.bio.net Dear NMR-ites, I don't know if anyone else has the same feelings as me but I sure am getting fed up of all the advertisments for web pages and stupid 'get rich quick' schemes that are hitting our beloved news group. Is there anyone who enjoys wading through all this rubbish, especially if they have been away from the lab for a day or two? Is there anything that can be done about it, or do we have to suffer in silence? Regards Mark Howard *********************************************************** Dr. Mark J. Howard NMR Spectroscopist University of Kent Canterbury Kent U.K. CT2 1JY Tel No: (01227) 764000 x7117/8 - Spectrometer Building x7012 - Office ************************************************************ From owner-structural-nmr@net.bio.net Tue May 27 23:00:00 1997 Path: biosci!CHEM.UNR.EDU!lcary From: lcary@CHEM.UNR.EDU (lew cary) Newsgroups: bionet.structural-nmr Subject: Re: Advertisments Date: 28 May 1997 11:24:31 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 5 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net There is a movement to legislate against junk email. A web search ought to find a contact to voice your opinion to. Newsweek, I think had a url listed. I am cautiously in favor of fineing spammers. Lew From owner-structural-nmr@net.bio.net Wed May 28 23:00:00 1997 Path: biosci!cnrs-orleans.fr!sodano From: sodano@cnrs-orleans.fr (Sodano Patrick) Newsgroups: bionet.structural-nmr Subject: (none) Date: 29 May 1997 00:21:26 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: <199705290720.JAA02100@admin.cnrs-orleans.fr> NNTP-Posting-Host: net.bio.net subscribe ************************************************************************* Dr Sodano Patrick Centre de Biophysique Mol=E9culaire Rue Charles Sadron 45071 Orl=E9ans C=E9dex 02 France ************************************************************************ From owner-structural-nmr@net.bio.net Wed May 28 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!europa.clark.net!news-peer.sprintlink.net!news.sprintlink.net!Sprint!howland.erols.net!torn!kone!news.ccs.queensu.ca!not-for-mail From: 4jch3@qlink.queensu.ca (Hesketh J Christian) Newsgroups: bionet.structural-nmr Subject: Ion Channel Web Page Date: 28 May 1997 13:43:09 GMT Organization: Queen's University, Kingston Lines: 20 Message-ID: <5mhcpd$72l@knot.queensu.ca> NNTP-Posting-Host: qlink.queensu.ca X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] The ion channel webpage has had a major update. Ion channel toxins, biophysical software, online ion channel articles have been added to the site. Direct molecular visualization is now offered allowing 3D rendered ion channel toxins to be viewed and rotated. In addition to these new features, the ion channel reseacher page, ion channel forum, ion channel reference list, Kv sequence analysis are also available. The URL for this site is: http://qlink.queensu.ca/~4jch3 Sincerely, --------------------------------------------------------------------------- J. Christian Hesketh Queen's University - Kingston, Canada Phone (613) 531-8048 e-mail: 4jch3@qlink.queensu.ca Web Page (Research in Ion Channels): http://qlink.queensu.ca/~4jch3 -Current research in ion channels with a biophysical perspective From owner-structural-nmr@net.bio.net Thu May 29 23:00:00 1997 Path: biosci!daresbury!not-for-mail From: Marc-Andre.Delsuc@cbs.univ-montp1.fr (M.A. Delsuc) Newsgroups: bionet.structural-nmr Subject: Professor position at University Paris 6 Date: 30 May 1997 18:19:13 +0100 Lines: 74 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <5mn26h$ger@mserv1.dl.ac.uk> X-Sender: mad@tome.cbs.univ-montp1.fr Original-To: str-nmr@dl.ac.uk Hi all, On behalf of Professor A. Marquet, from the University Pierre et Marie Curie= , I am sending to you this information about a position in this university. Please do not contact me for more information about this position, but rather contact directly A.Marquet. Best regards _________________________________________________________________________ Marc-Andre' Delsuc Centre de Biochimie Structurale Marc-Andre.Delsuc@cbs.univ-montp1.fr Faculte de Pharmacie tel : (33) (0) 467 04 34 36 15 av, Charles Flahault fax : (33) (0) 467 52 96 23 34060 Montpellier cedex www : http://www.cbs.univ-montp1.fr FRANCE -----text of the announcement--------------- Le D=E9partement de Chimie Organique de l'Universit=E9 Pierre et Marie Curie (Paris 6) recherche des candidats, sp=E9cialistes de RMN, =E0 un poste de Professeur. Une comp=E9tence dans l'=E9tude des (macro)mol=E9cules biologiques,= structure et interactions, est souhait=E9e. Les candidats devraient =EAtre int=E9ress=E9s par l'enseignement =E0 tous le= s niveaux et assurer =E0 c=F4t=E9 des cours sp=E9cialis=E9s de RMN des cours= de chimie, organique ou g=E9n=E9rale au niveau 1er ou 2=E8me cycle. Contacter : Prof. A. Marquet Laboratoire de Chimie Organique Biologique Universit=E9 Paris 6 Tour 44-45 - 3=E8me =E9tage - Bo=EEte Courrier n=B0 182 4, place Jussieu 75252 PARIS CEDEX 05 T=E9l. : 01 44 27 55 35 =46ax : 01 44 27 71 50 e.mail : marquet@ccr.jussieu.fr The Organic Chemistry Department at the Pierre & Marie Curie University (Paris 6) is seeking for candidates specializing in NMR, for a full professorial position. An expertise in biological macromolecule structure and interactions would be an advantage. Candidates should have a strong interest in both undergraduate and graduate teaching in NMR and either organic or general chemistry. The successful candidate would be expected to be able to teach in French. Please send a curriculum vitae and a description of research interests to : Prof. A. Marquet Laboratoire de Chimie Organique Biologique Universite Paris 6 Tour 44-45 - 3eme etage - Boite Courrier n=B0 182 4, place Jussieu 75252 PARIS CEDEX 05 phone. : (33) 1 44 27 55 35 =46ax : (33) 1 44 27 71 50 e.mail : marquet@ccr.jussieu.fr From owner-structural-nmr@net.bio.net Thu May 29 23:00:00 1997 Path: biosci!HEMEBASE.BIO.UCI.EDU!raman From: raman@HEMEBASE.BIO.UCI.EDU (CS Raman) Newsgroups: bionet.structural-nmr Subject: Newsgroup Moderation Date: 29 May 1997 18:52:03 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 17 Sender: daemon@net.bio.net Distribution: world Message-ID: <199705300153.BAA04320@hemebase.bio.uci.edu> NNTP-Posting-Host: net.bio.net I would like to inform everyone that we are in the process of being fully moderated and it should take effect within the next week. I appreciate the patience of the readership in this regard. Cheers -raman ___________________________________________________________________ C.S.Raman Tel: (714) 824-4322 University of California Fax: (714) 824-8540 Dept. MB & B email: raman@indigo1.bio.uci.edu 3205 Bio Sci II Irvine, CA 92697-3900 ------------------------------------------------------------------- In a time of drastic change it is the learners who inherit the future. The learned usually find themselves equipped to live in a world that no longer exists. --Eric Hoffer ___________________________________________________________________ From owner-structural-nmr@net.bio.net Thu May 29 23:00:00 1997 Path: biosci!daresbury!is.bbsrc.ac.uk!news.ox.ac.uk!lyra.csx.cam.ac.uk!hgmp.mrc.ac.uk!news From: Robin Munro Newsgroups: bionet.structural-nmr Subject: Announce: DRAGON Version 4.17.6 is now available Date: Fri, 30 May 1997 12:12:44 +0100 Organization: NIMR Lines: 28 Message-ID: <338EB62C.2781@nimr.mrc.ac.uk> NNTP-Posting-Host: glycine.nimr.mrc.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01 (X11; I; IRIX 6.2 IP22) ---- ATTENTION ALL PROTEIN MODELLERS ---- DRAGON Version 4.17.6 is now available in SGI executable format at the following site:- http://www.nimr.mrc.ac.uk/~mathbio/a-aszodi/dragon.html DRAGON is a protein modelling tool using Distance Geometry. It was developed at the Division of Mathematical Biology of the National Institute for Medical Research in London between 1993 and 1996. DRAGON attempts to predict the tertiary structure of a small soluble protein, given its sequence, the secondary structure and possibly a set of interresidue distance restraints. If the structures of some of the sequences in the multiple alignment is known, then you can attempt comparative modelling. DRAGON communicates with you through a simple command-line interface which is used to specify parameter values and input filenames. Further information and references can be found at:- http://www.nimr.mrc.ac.uk/~mathbio/a-aszodi/dragon.html#moinfo http://www.nimr.mrc.ac.uk/~mathbio/r-munro/dragon.html Enjoy! Andras Aszodi (PS. This message was posted by Robin Munro on behalf of A.A.)