From owner-structural-nmr@net.bio.net Mon Sep 01 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Istvan Pelczer Newsgroups: bionet.structural-nmr Subject: exch Date: 2 Sep 1997 10:02:31 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 17 Sender: daemon@net.bio.net Approved: raman@hemebase.bio.uci.edu Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear Netters, Could you advise me on some easily available software package which could be used to calculate exchange reates in a multisite system based on 2D EXSY data? Thanks in advance, Istvan wwww,wwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwww Istvan Pelczer, Ph.D. Email: ipelczer@princeton.edu Senior NMR Spectroscopist Princeton University Department of Chemistry, Frick Lab., ph# (609) 258 2342 Washington Road fax# (609) 258 6746 Princeton, NJ 08544, USA From owner-structural-nmr@net.bio.net Thu Sep 04 23:00:00 1997 Path: biosci!biosci!not-for-mail From: "Lowman, Douglas W" Newsgroups: bionet.structural-nmr Subject: Job Opening at Eastman Chemical Company Date: 5 Sep 1997 13:18:17 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 76 Sender: daemon@net.bio.net Approved: raman@hemebase.bio.uci.edu Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net --Boundary (ID WnRlyFxskdSCFT1mw7qD+Q) Content-type: text/plain; charset="us-ascii" Content-transfer-encoding: 7bit I am sending this job posting to two mail lists. Please accept our apologies for any inconvenience due to multiple copies that you might receive. Please send all correspondence to the address listed below, not to me. We request that all resumes be sent by US mail (i.e., snail mail) to the address given below. We prefer not to receive resumes by FAX or by e-mail. Please provide the Job Code reference number given at the end of this notice in your cover letter and on the envelope of your correspondence. Posting date: September 5, 1997. PhD NMR Spectroscopist Technological Innovation Group Spectrometry Applications Research Lab The Research Laboratories of Eastman Chemical Company has an opening for a Ph.D. level NMR spectroscopist with a proven performance record in the interpretation of 1D and nD solution-state NMR spectra of synthetic, industrial polymers, cellulosic materials, and organic molecules. The focus of this laboratory is the interpretation of NMR spectra to effectively and efficiently solve structural analysis problems. Working knowledge of state-of-the-art solution-state high-field NMR techniques, including 1D and nD homo- and hetero-nuclear NMR as well as hardware and software troubleshooting skills are imperative. The successful candidate must be interested in working in a problem-solving environment, exhibit good communications skills, and be a self-starter with a desire to work both as a contributing member of a team and as an individual contributor. Eastman Chemical Company, which employs approximately 350 PhD scientists and engineers, is one of the largest chemical and fiber manufacturing facilities in the U. S. The company's central research laboratories are at the Kingsport location and provide a variety of resources for the technical community. Kingsport is located in the Tri-Cities area of northeast Tennessee in the foothills of the Smoky Mountains. The area is rated among the top 25 most livable metropolitan areas and offers affordable housing, low taxes, and excellent schools, including a nearby state university. The pleasant four-season climate permits a variety of outdoor recreational opportunities, such as boating and fishing in the numerous lakes and streams or skiing and hiking in the nearby mountains. Interested candidates should send resume to: EASTMAN CHEMICAL COMPANY Employment Department P. O. Box 1975 Kingsport, TN USA 37662-5215 Please reference Job Code: E9-98-KPT-TI-SM-1. An Equal Opportunity Employer ====================================================== Douglas W. Lowman, Research Associate Physical and Analytical Chemistry Research Division Technological Innovation Group, Eastman Chemical Company P. O. Box 1972, Building 95, 200 South Wilcox Drive Kingsport, TN 37662-5150 USA e-mail: dwlowman@eastman.com Telephone: (423) 229-4728 FAX: (423) 229-4558 http://www.eastman.com ======================================================= From owner-structural-nmr@net.bio.net Tue Sep 09 23:00:00 1997 Path: biosci!biosci!not-for-mail From: felix97 Newsgroups: bionet.structural-nmr Subject: FELIX 97.0 Workshop Series Date: 10 Sep 1997 10:31:43 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 33 Sender: daemon@net.bio.net Approved: raman@hemebase.bio.uci.edu Distribution: world Message-ID: <3.0.16.19970910092243.405f481c@146.202.6.217> NNTP-Posting-Host: net.bio.net Molecular Simulations, Inc. and Silicon Graphics present FELIX 97.0 Workshop Series Felix 97.0 THE NEW GENERATION OF NMR SOFTWARE New and Improved - Easy to use - More streamlined This workshop series will introduce you to the new features in FELIX 97.0, and give you hands-on experience for analyzing your own data. To register for one of the workshops, visit the special promotions area of our Website (www.msi.com) ,send an email to felix97@msi.com, or cal (619) 546-5568. September 15, San Diego, CA September 17, Mountain View, CA September 19, Schaumburg, Il September 22, Research Triangle Park, NC September 24, Philadelphia, PA September 26, Boston, MA October 6, Cambridge, UK October 8, Munich, Germany October 10, Paris, France FELIX 97.0 Workshop Series Phone: (619) 546-5568 Fax: (619) 597-9777 Email: felix97@msi.com Web: http://www.msi.com From owner-structural-nmr@net.bio.net Tue Sep 09 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Steve Pascal Newsgroups: bionet.structural-nmr Subject: NMR postdoctoral position Date: 9 Sep 1997 18:55:22 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 32 Sender: daemon@net.bio.net Approved: raman@hemebase.bio.uci.edu Distribution: world Message-ID: <9709092030.AA05587@oxbow.biophysics.rochester.edu> NNTP-Posting-Host: net.bio.net A postdoctoral position will be available in my laboratory beginning this fall/winter, to study the structure and dynamics of protein complexes involved in transcriptional regulation. Within the last two years, the Department of Biochemistry and Biophysics at the University of Rochester has hired two NMR spectroscopists, an X-ray crystallographer and installed a fully equipped Varian Unity+ 600 MHz spectrometer as part of a structural biology initiative. Applicants with experience in multi-nuclear NMR spectroscopy and/or protein expression/purification should send a CV along with telephone numbers and email addresses of three people familiar with their work to the address below. Email responses are encouraged. Thank You =========================================== Steve Pascal Assistant Professor Box 712 Department of Biochemistry & Biophysics University of Rochester Medical Center 601 Elmwood Ave Rochester, NY 14642 716-273-4832 (voice) 716-275-6007 (fax) =========================================== From owner-structural-nmr@net.bio.net Thu Sep 11 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Alfred Ross Newsgroups: bionet.structural-nmr Subject: Relaxation agens Date: 12 Sep 1997 13:20:16 -0700 Organization: Roche Basel AG Lines: 28 Sender: daemon@net.bio.net Approved: raman@hemebase.bio.uci.edu Distribution: world Message-ID: <34190F0B.41C6@roche.com> NNTP-Posting-Host: net.bio.net Dear NMR-netters, does anybody know a relaxation agens that can be dissolved in high enough concentration in CDCl3 to shorten the T1 relaxation of 13C down to about 1s? Thanks in advance Alfred -- ************************************* Dr. Alfred Ross NMR-Spectroscopist e-mail: alfred.ross@roche.com ******* Phone: CH-(0)61-6887029 * * Fax: CH-(0)61-6887408 * ROCHE * * * Mail: F. Hoffmann-LaRoche AG ******* (A. Ross - PRPI-S) Postfach CH-4070 Basel ************************************* From owner-structural-nmr@net.bio.net Thu Sep 11 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Laura G Barrientos Newsgroups: bionet.structural-nmr Subject: 3D TOCSY-TOCSY Date: 12 Sep 1997 13:19:58 -0700 Organization: Chemistry, Carnegie Mellon, Pittsburgh, PA Lines: 8 Sender: daemon@net.bio.net Approved: raman@hemebase.bio.uci.edu Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Hello, Can someone help me getting either a reference for a pulse sequence with phase cycling and/or the Bruker macro for a 3D TOCSY-TOCSY experiment? Thanks in advance, Laura. From owner-structural-nmr@net.bio.net Thu Sep 11 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Frank Soennichsen Newsgroups: bionet.structural-nmr Subject: Post doctoral positions Date: 12 Sep 1997 13:20:40 -0700 Organization: Case Western Reserve University Lines: 45 Sender: daemon@net.bio.net Approved: raman@hemebase.bio.uci.edu Distribution: world Message-ID: <341071B0.1075@herring.phol.cwru.edu> Reply-To: frank@herring.phol.cwru.edu NNTP-Posting-Host: net.bio.net Post-Doctoral Fellowships in Biological NMR Spectroscopy At Case Western Reserve University, F. S=F6nnichsen Applications are invited for post-doctoral positions available immediately in our laboratory. The positions are tenable for up to three years, with one position having the potential to lead to a permanent full-time facility manager position. Successful candidates will be responsible for the characterization of structural, dynamic and functional properties of proteins. Methods will primarily include multi-dimensional NMR spectroscopy, as well as other biophysical techniques. Candidates should have a strong background in NMR spectroscopy, and have experience with solution structure determination, computational techniques, or peptide synthesis and molecular biology.=20 Candidates with solid-state NMR background will also be considered. Our laboratory is part of the Cleveland Center for Structural Biology. We have unlimited access to the new NMR-facilities of the Center, consisting of four and three-channel Varian Inova 600, 500 and 300 spectrometers fully equipped for biomolecular studies. The center is located within a 10 minute walk away from our laboratory, which is located in the School of Medicine at Case Western in the recently renovated Department of Physiology and Biophysics. The lab currently comprises 4 post-docs and graduate students, is computationally well equipped with several SGI Indigo2 workstations as well as Macs and PCs, and all relevant academic or commercial software.=20 Proteins currently under investigations are antifreeze proteins from various fish species. Particular research focus is their protein structure, dynamics and stability, as well as the characterization of their interaction with ice. Further areas include structure and function studies of carboxylase domains, and pheromone/odorant binding proteins. For more information visit our home page at URL: http://pout.phol.cwru.edu/~frank/home.html, or=20 http://ccsb.base.org Applicants should mail or e-mail a curriculum vitae, and the names, addresses, and telephone numbers of three referees to: Dr. Frank D. S=F6nnichsen Department of Physiology and Biophysics Case Western Reserve University 10900 Euclid Avenue Cleveland, OH 44106-4970 e-mail : frank@herring.phol.cwru.edu From owner-structural-nmr@net.bio.net Mon Sep 15 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Istvan Pelczer Newsgroups: bionet.structural-nmr Subject: NMR Position (fwd) Date: 16 Sep 1997 16:32:41 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 42 Sender: daemon@net.bio.net Approved: raman@hemebase.bio.uci.edu Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net This message is in MIME format. The first part should be readable text, while the remaining parts are likely unreadable without MIME-aware tools. Send mail to mime@docserver.cac.washington.edu for more info. --IMA.Boundary.550200478 Content-Type: TEXT/PLAIN; CHARSET=US-ASCII Content-ID: Content-Description: cc:Mail note part Dear Netters, Please, find below a message about a job opening, which I transfer according to the request of my colleague. Best regards, Istvan wwww,wwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwww Istvan Pelczer, Ph.D. Email: ipelczer@princeton.edu Senior NMR Spectroscopist Princeton University Department of Chemistry, Frick Lab., ph# (609) 258 2342 Washington Road fax# (609) 258 6746 Princeton, NJ 08544, USA __________________________________________________ POSITION AVAILABLE - NMR SPECTROSCOPY There is an opening for a NMR spectroscopist at the Lonza, Inc. R&D center in Clinton, NJ. The candidate should have a Ph.D. in chemistry with a strong background in NMR spectroscopy. This person will be responsible for a molecular characterization group that includes NMR, IR, GC-MS, and electrospray-LC/MS. The person will also supervise two chemists. Lonza Inc. is a leading manufacturer of biocides, surfactants, and other specialty chemicals. Lonza is the Chemicals Division of the Alusuisse-Lonza Group (Switzerland) that employs over 5,000 people in 20 countries worldwide. Please contact Dr.Molly Crowther, Lonza Inc., (908)-730-1593 --IMA.Boundary.550200478-- From owner-structural-nmr@net.bio.net Mon Sep 15 23:00:00 1997 Path: biosci!biosci!not-for-mail From: "Chuck Sanders" Newsgroups: bionet.structural-nmr Subject: expression at room temp? Date: 16 Sep 1997 16:32:05 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Approved: raman@hemebase.bio.uci.edu Distribution: world Message-ID: <9709161711.ZM6471@lyotrope.PHOL.CWRU.Edu> NNTP-Posting-Host: net.bio.net Hi Folks, We are trying to grow and induce E. Coli at room temperature using both Luria and minimal media. Getting the cells to an OD600 of 1.0 is no problem. However, the question is how long to let the cells go after inducing and whether to do anything non-standard at this stage (like re-adding IPTG every N hours to keep induction going). Does anyone have any comments on this? Also, has anyone compared the yield of proteins from RT cultures to the yield at 30 or 37 degrees? Thanks! Chuck Sanders, CWRU From owner-structural-nmr@net.bio.net Thu Sep 18 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Rasmus Storjohann Newsgroups: bionet.structural-nmr Subject: Distance restraints from ring-current shift Date: 19 Sep 1997 14:54:13 -0700 Organization: Simon Fraser University Lines: 26 Sender: daemon@net.bio.net Approved: raman@hemebase.bio.uci.edu Distribution: world Message-ID: References: <34227B6B.67B0@nmr1.ioc.ac.ru> NNTP-Posting-Host: net.bio.net I observe a significant ring-current shift both for the alpha and beta protons of Ser61 originating from Tyr63. When I do structure calculations using X-plor the serine is nowhere near the tyrosine ring since X-plor puts this region in an alpha-helical conformation. Somehow I want to include the information of the ring-current shift, but I don't know what would be the best way of doing that. I've tried the obvious thing which is to include distance restraints from the Ser HG to atoms in the Tyr ring (I chose CZ and CD*). Is this the best thing to do, or does anybody have something better to suggest. Pointers to relevant publications would also be appreciated. Thanks Rasmus __ Rasmus Storjohann / /\ Institute of Molecular Biology / / \ and Biochemistry / / /\ \ Simon Fraser University / / /\ \ \ Burnaby, British Columbia V5A 1S6 / /_/__\ \ \ e-mail: rstorjoh@sfu.ca /________\ \ \ Phone: (604) 291-5657 / 415-0575 \___________\/ FAX: (604) 291-3765 From owner-structural-nmr@net.bio.net Thu Sep 18 23:00:00 1997 Path: biosci!biosci!not-for-mail From: "Zatonskiy George V." Newsgroups: bionet.structural-nmr Subject: heteronuclear 3J(ch) coupling constant Date: 19 Sep 1997 13:44:35 -0700 Organization: Institute of Organic Chemistry Russian Academy of Science Lines: 19 Sender: daemon@net.bio.net Approved: raman@hemebase.bio.uci.edu Distribution: world Message-ID: <34227B6B.67B0@nmr1.ioc.ac.ru> NNTP-Posting-Host: net.bio.net Dear Thomas, try SERF (M.Eberstadt, G.Gemmecker, D.F. Mierke, H.Kessler, Angew.Chem.Int.Ed.Engl. 1995, 34,1671-1695). It seems to be you would like. This experiment discribed in NMR "cookbook" - S.Braun, H.-O.Kalinowski, S.Berger, "100 and More Basic NMR Experiments", p.212-214. If you have Bruker AMX or later spectrometer, you can get pulseprogram from Bruker ftp server (ftp.bruker.de) -- George V.Zatonskiy NMR laboratory N.D. Zelinsky Institute of Organic Chemistry, Leninsky pr., 47 Moscow 117913 Russia e-mail: zatonsk@nmr1.ioc.ac.ru tel.: (7-095) 135-9094 fax : (7-095) 135-5328 From owner-structural-nmr@net.bio.net Fri Sep 19 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Istvan Pelczer Newsgroups: bionet.structural-nmr Subject: Re: Distance restraints from ring-current shift Date: 20 Sep 1997 09:56:13 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 51 Sender: daemon@net.bio.net Approved: raman@hemebase.bio.uci.edu Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear Rasmus, I have no comment on the X-plor protocol itself, however, if you would search for papers of David Case (Scripps) and Mike Williamson (Sheffield) -- and also Brian Sykes -- , they have presented calculations on chemical shifts in proteins, including ring-current effects to my best recollection. Their results could give you some input how to approach the effects you observe. Hope it helps a bit, Cheers, Istvan wwww,wwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwww Istvan Pelczer, Ph.D. Email: ipelczer@princeton.edu Senior NMR Spectroscopist Princeton University Department of Chemistry, Frick Lab., ph# (609) 258 2342 Washington Road fax# (609) 258 6746 Princeton, NJ 08544, USA On Fri, 19 Sep 1997, Rasmus Storjohann wrote: > > I observe a significant ring-current shift both for the alpha and beta > protons of Ser61 originating from Tyr63. When I do structure calculations > using X-plor the serine is nowhere near the tyrosine ring since X-plor > puts this region in an alpha-helical conformation. Somehow I want to > include the information of the ring-current shift, but I don't know what > would be the best way of doing that. I've tried the obvious thing which > is to include distance restraints from the Ser HG to atoms in the Tyr > ring (I chose CZ and CD*). Is this the best thing to do, or does anybody > have something better to suggest. Pointers to relevant publications would > also be appreciated. > > Thanks > Rasmus > > __ Rasmus Storjohann > / /\ Institute of Molecular Biology > / / \ and Biochemistry > / / /\ \ Simon Fraser University > / / /\ \ \ Burnaby, British Columbia V5A 1S6 > / /_/__\ \ \ e-mail: rstorjoh@sfu.ca > /________\ \ \ Phone: (604) 291-5657 / 415-0575 > \___________\/ FAX: (604) 291-3765 > > > > From owner-structural-nmr@net.bio.net Mon Sep 22 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Yu Wai Chen Newsgroups: bionet.structural-nmr Subject: question about EPR Date: 23 Sep 1997 09:00:01 -0700 Organization: MRC Centre for Protein Engineering Lines: 15 Sender: daemon@net.bio.net Approved: raman@hemebase.bio.uci.edu Distribution: world Message-ID: <608p21$prt@net.bio.net> NNTP-Posting-Host: net.bio.net Dear all, I am interested in obtaining structural information about a protein which forms aggregates in physiological conditions. Do you think I can use site-directed spin labelling and then EPR to get side-chain mobility data? Can EPR be run on aggregated samples? What other techniques would you suggest? Thanks. Any advice would be appreciated. -- =================================================================== Yu Wai CHEN, Ph.D. .................. email: ywc@mrc-lmb.cam.ac.uk Centre for Protein Engineering, tel: 44-(1223) 402148 MRC Centre, Cambridge CB2 2QH, U.K. fax: 44-(1223) 402140 WWW homepage: http://www.mrc-cpe.cam.ac.uk/people/wai.html From owner-structural-nmr@net.bio.net Mon Sep 22 23:00:00 1997 Path: biosci!biosci!not-for-mail From: lukasz@wlheye.jsei.ucla.edu (Lukasz Salwinski) Newsgroups: bionet.structural-nmr Subject: Re: question about EPR Date: 23 Sep 1997 12:59:46 -0700 Organization: Jules Stein Eye Institute, UCLA Lines: 21 Sender: daemon@net.bio.net Approved: raman@hemebase.bio.uci.edu Distribution: world Message-ID: <6095o9$g4g@uni.library.ucla.edu> References: <608p21$prt@net.bio.net> Reply-To: lukasz@jsei.ucla.edu NNTP-Posting-Host: net.bio.net >I am interested in obtaining structural information about a protein >which forms aggregates in physiological conditions. Do you think I can >use site-directed spin labelling and then EPR to get side-chain mobility >data? unless you end up with some non specific aggregate (aka precipitate ;o) it should be ok. The only drawback is one gets mobility/exposure data for the aggregate, not monomer. >Can EPR be run on aggregated samples? no problem. there's no molecular weight limit. >What other techniques would you suggest? fluorescence (tryptophane) labeling and blue shift/polarisation anisotropy ? -- lukasz salwinski From owner-structural-nmr@net.bio.net Tue Sep 23 23:00:00 1997 Path: biosci!biosci!not-for-mail From: David Gorenstein Newsgroups: bionet.structural-nmr Subject: announcement of NMR position Date: 24 Sep 1997 13:55:20 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 68 Sender: daemon@net.bio.net Approved: raman@hemebase.bio.uci.edu Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Please post and distribute: Nuclear Magnetic Resonance Spectroscopy Biophysical Faculty Position The University of Texas Medical Branch at Galveston, Texas In continuing development of Structural Biology at the University of Texas Medical Branch, we are pleased to announce a tenure-track faculty opening at the Assistant Professor level. We seek candidates with strong backgrounds in NMR spectroscopy who make extensive use of NMR to solve important biological problems. The successful candidate is to establish an independent research program that may involve determination of macromolecularstructures and/or innovative application of NMR spectroscopy in the study of biological macromolecules. The NMR facility at UTMB consists of new 400, 600 and 750 MHz Varian spectrometers housed in a newly renovated building. Candidates must have outstanding potential in research with a record and stature in their field of expertise. They are expected to attract funding from national agencies and to fully participate in teaching and service with the Department of Human Biological Chemistry and Genetics. Affiliations with The Sealy Center for Structural Biology, other UTMB Centers and relevant Departments are possible. Structural Biology currently consists of 10 biophysical faculty members in areas of NMR spectroscopy, x-ray crystallography, computational biology, and biophysical chemistry with facilities directed by Drs. David Gorenstein, Werner Braun, Robert O. Fox and James Lee, respectively. Structural Biology received financial support from UTMB and endowed support from the Sealy Center for Structural Biology. Facilities consist of a newly renovated building andadditional laboratories, along with excellent computational, NMR, crystallographic, and biophysical equipment. Applications should include a complete curriculum vitae, a description of research interests and names and addresses of at least three references. Review of applications will begin October 15, 1997 and continue until the position is filled. Applications should be addressed to: Dr. James C. Lee Structural Biology Search Committee Department of Human Biological Chemistry and Genetics The Sealy Center for Structural Biology University of Texas Medical Branch Galveston, Texas 77555-1055 UTMB is an equal opportunity M/F/V/D affirmative action employer. UTMB hires onlyindividuals authorized to work in the United States. Thank you, ----------------------------------------------------------- David Gorenstein Director, Sealy Center for Structural Biology Charles Marc Pomerat Distinguished Professor of Biological Sciences, Dept. of Human Biological Chemistry and Genetics Dept. of Physiology and Biophysics University of Texas Medical Branch Galveston, TX 77555-1157 Office: (409) 747 6801 Main Office: (409) 747 6800 Voice Mail: (409) 747 6801 Fax: (409) 747 6850 E-MAIL ADDRESS: david@nmr.utmb.edu http://www.nmr.utmb.edu/ From owner-structural-nmr@net.bio.net Fri Sep 26 23:00:00 1997 Path: biosci!biosci!not-for-mail From: "Barbara Wright" Newsgroups: bionet.structural-nmr Subject: Data Conference Date: 27 Sep 1997 11:34:58 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 537 Sender: daemon@net.bio.net Approved: raman@hemebase.bio.uci.edu Distribution: world Message-ID: <8525651C.00573F5D.00@smtpmta.nas.edu> NNTP-Posting-Host: net.bio.net Dear colleague: This is a reminder for readers of your Web site about the upcoming Conference on Scientific and Technical Data Exchange and Integration. If you have not yet posted an announcement about it there, please do so at your earliest convenience. You can use the following message: +++++++++++++++++++++++ The Conference on Scientific and Technical Data Exchange and Integration will be held December 15-17, 1997 at the Natcher Conference Center in Bethesda, MD just outside Washington, D.C. The conference is being organized by the National Research Council's U.S. National Committee for CODATA and is being cosponsored by nine federal science agencies and several firms. Information about the program, registration, and local arrangements may be obtained by calling (202) 334-2124, sending an e-mail inquiry to , or by visiting the Web site for the conference at . ++++++++++++++++++++++++ We also are providing below the full text of the revised conference announcement for your information. Please share it with others in your organization who may be interested. Thank you. Paul F. Uhlir Director, U.S. National Committee for CODATA National Research Council Washington, DC +++++++++++++++++++++++++++++++++++++++++++++ CONFERENCE ANNOUNCEMENT (Revised September 1997) The Conference on Scientific and Technical Data Exchange and Integration Sponsored by U.S . National Committee for CODATA National Research Council December 15-17, 1997 Natcher Conference Center National Institutes of Health Bethesda, MD Purpose of the Conference The exchange of scientific and technical (S&T) data among different computing environments and across diverse scientific and engineering disciplines presents major problems that hinder full exploitation of computer-based modeling, the Internet, modern scientific databases, and new computer technology. The U.S. National Committee for CODATA is sponsoring the first major interdisciplinary conference on this subject on December 15-17, 1997, in Bethesda, MD. The conference has three main objectives: - To identify areas, with special emphasis on interdisciplinary needs, in which data exchange and integration are important; - To highlight major S&T data exchange and integration efforts already underway or in planning; and - To foster serious and significant cooperation in these kinds of activities among scientific and engineering disciplines, and governmental and non-governmental organizations. Conference Sponsors Defense Technical Information Center Department of Energy Environmental Protection Agency National Aeronautics and Space Administration National Institutes of Health National Institute of Standards and Technology National Oceanic and Atmospheric Administration National Science Foundation United States Geological Survey Hughes STX Corporation TRW [Additional government and corporate sponsors welcome] Preliminary Program Monday, December 15, 1997 8:40 a.m. Welcome Goetz Oertel, U.S. National Committee for CODATA 8:45 Conference Introduction William Wulf, National Academy of Engineering Plenary Session 1: The Importance of Scientific Data Sharing 9:00 Sharing Scientific Data--A Key to Progress in Research and Development Rita Colwell, University of Maryland Biotechnology Institute 9:30 Getting More from Our Research Investment--Cross-discipline Research and Data Sharing Neal Lane, National Science Foundation 10:00 Data Exchange and Integration--Fundamental Issues John Rumble, National Institute of Standards and Technology 10:30 Coffee 10:50 An Industrial Perspective: Why Industry Shares Scientific and Technical Data, and How Robert Kiggans, PDES, Inc. 11:20 The Need for Data Exchange in Global Change Research Robert Corell, National Science Foundation 11:50 Lunch 1:00 p.m. Contributed Papers, Posters, and Demonstrations (available for viewing until noon on Wednesday) Plenary Session 2: Tearing Down the Walls--The Art and Science of Data Exchange and Integration 3:00 Data Exchange and Integration Approaches Gio Wiederhold, Stanford University 3:30 Information Modeling Yuhwei Yang, Product Data Integration Technology 4:00 Resolving Conceptual Disagreements Frank Olken, Lawrence Berkeley Laboratory 4:30 The Sociology of Data Exchange--Reaching Consensus on Data Exchange Tools G. Bruce Wiersma, University of Maine at Orono 5:00 Making Data Easy to Share Ben Shneiderman, University of Maryland 5:30 Reception 7:30 Adjourn Tuesday, December 16 Plenary Session 3: Challenges to Cooperation--Why Data Exchange Must Succeed 9:00 a.m. Sharing Scientific and Technical Data--Maximizing the Potential of the National Information Infrastructure Senior Administration Official 9:45 Long-term Ecological and Environmental Data--The Challenge of Keeping and Remembering Susan Stafford, Oregon State University 10:10 Space Observation Data: Looking in and Looking out Jim Green, National Aeronautics and Space Administration 10:35 Coffee 11:00 Human Health and Global Climate Change Paul Epstein, Harvard University 11:25 Geographic Information: What Everybody Needs, and Why David Mark, University of Buffalo 11:50 Molecular and Cellular Bioinformatics: From Molecules to Biological Functionality David Lipman, National Center for Biotechnology Information 12:15 p.m. Lunch 1:15 Break-Out Discussion Group Sessions (topics to be finalized later) 2:45 Coffee 4:15 Conclusion of Break-Out Sessions Plenary Session 3: Challenges to Cooperation (continued) 4:30 Integrating Social Science and Natural Science Data Roberta Miller, Consortium for International Earth Science Information Network 5:00 Legal Challenges to Data Exchange and Integration Paul Uhlir, National Research Council 5:30 Adjourn Wednesday, December 17 Plenary Session 4: How to Cooperate--Examples of Successful Cross-Discipline Data Exchange and Integration 8:45 a.m. Geographic Information Systems John Moeller, U.S. Geological Survey and Federal Geographic Data Committee 9:05 ISO Standard for the Exchange of Product Data Howard Bloom, National Institute of Standards and Technology 9:25 World Data Centers Ferris Webster, University of Delaware 9:45 The Earth Observing System Gregory Hunolt, National Aeronautics and Space Administration 10:05 Coffee Closing Plenary Session 10:30 Ideas from the Break-Out Sessions Julian Humphries, University of Kansas 10:50 Next Steps for Working Scientists Robert Robbins, Fred Hutchinson Cancer Research Center 11:20 Next Steps for the Federal Research Community Senior Official, Office of Science and Technology Policy 11:45 Final Remarks Goetz Oertel, U.S. National Committee for CODATA Noon Adjourn Contributed Papers and Technical Demonstrations The conference will consist of four types of sessions: plenary invited lectures; contributed papers (which will be presented as posters); technical demonstrations and exhibits; and small break-out discussion groups. Case studies are particularly encouraged. Contributed papers and demonstrations are being provided on the following topics: Discipline-specific data exchange activities and requirements Interdisciplinary data exchange activities and requirements Federally supported data exchange programs Definitions of scientific and technical metadata issues The computer science of data exchange and integration The impact of the Internet and the World Wide Web on S&T data exchange and integration Future needs for data exchange and integration for scientific and technical data The contributed papers and technical demonstrations will play a major role in the conference by identifying existing activities and approaches that will provide direction and insight for further activities. All contributed papers will be considered for publication in the Conference proceedings, which will be published on the Internet soon after the Conference. The abstracts for all accepted contributed papers and technical demonstrations and exhibits will be put on our Web site in October at . For further information about the conference, please contact: Paul F. Uhlir Director, U.S. National Committee for CODATA National Research Council 2101 Constitution Avenue, NW Washington, DC 20418 (202) 334-2421 (tel.) (202) 334-2422 (fax) codataco@nas.edu For questions about the conference program, please contact: John Rumble Conference Program Chair National Institute of Standards and Technology Building 820, Room 113 Gaithersburg, MD 20899 (301) 975-2200 (tel.) john.rumble@nist.gov Break-out Discussion Group Sessions The purpose of the small group discussions, which will be held on the afternoon of the second day, is to address focused topics within the broad conference themes. All conference participants are invited to participate in a discussion group of their choice. Each group will have a designated chair and rapporteur, who will lead and record the discussion. The results will be used by the U.S. National Committee for CODATA and the other conference sponsors for planning follow-on activities. The discussion groups are expected to examine data exchange and integration issues in the following discipline and issue areas: - Biodiversity - Biophysics - Bioinformatics - Engineering knowledge systems - Industrial data - Space sciences - Earth observations - Geographic information - Social sciences - Intellectual property rights - International cooperation - Computer science - Long-term archiving Further details about these discussion groups will be made available in October. Additional Background By data exchange is meant several things: the transfer of large amounts of data from one set of software to other software; extracting small amounts of data from one or more data sources for specific use; and the creation of a linked or integrated data system with multiple data sources. Other possibilities exist. Data exchange has two major components: the stream of bits and bytes that actually represent the data items and fields, and the contextual meaning of individual data items and fields. S&T disciplines and applications have begun addressing data exchange issues, but progress has been slow and difficult for a variety of reasons. Scientists are often not accustomed to formal standards. Discipline experts, even though they may be quite knowledgeable in computation and database management, frequently lack expertise in information modeling and exchange standards. Metadata are not well defined, complicating the application of data across diverse scientific areas. As a result, interdisciplinary data exchange has been difficult to promote and rarely implemented. Consider for a moment geographic information. Many applications need such information: to locate physically the sources of samples, to describe the range of a phenomenon, or to specify the location of an event, among others. Today many geographic information systems serve diverse communities of users, and several efforts to develop standards for exchanging data among these systems have been proposed. Yet progress to develop such standards in other areas has been slow. Other types of scientific data, such as biological nomenclature, chemical and engineering material identification and temporal data, suffer the same problem. Many uses for these data exist outside the scientific disciplines that generate them, yet accepted methods for exchanging these data remain elusive. In Finding the Forest in the Trees, The Challenge of Combining Diverse Environmental Data, the U.S. National Committee for CODATA clearly documented case studies in which data interfacing, defined in that report as the coordination, combination or integration of data for the purpose of modeling, correlation, pattern analysis, hypotheses testing, and field investigation at various scales, was necessary to achieve full value of research investment. Data interfacing is founded upon the standards and protocols agreed to by different scientific disciplines to exchange data. Particular emphasis must be put on the role of metadata in this data exchange. About CODATA The Committee on Data for Science and Technology (CODATA) is an interdisciplinary committee organized under the International Council of Scientific Unions (ICSU). CODATA is concerned with all types of quantitative data resulting from experimental measurements or observations in the physical, biological, geological, and astronomical sciences. Particular emphasis is given to data management problems common to different scientific disciplines and to data used outside the field in which they were generated. The general objectives are the improvement of the quality and accessibility of data, as well as the methods by which data are acquired, managed, and analyzed; the facilitation of international cooperation among those collecting, organizing, and using data; and the promotion of an increased awareness in the scientific and technical community of the importance of these activities. The U.S. National Committee for CODATA is organized by the National Research Council to administer activities within the United States related to CODATA. The Committee is funded by several federal agencies. Over the past decade, the Committee has completed several studies that have identified and analyzed issues related to maximizing the availability and usability of scientific and technical data. This national conference builds upon those studies and is intended to spur further progress and cooperation in data exchange and integration. Local Information Location The conference will be held at the Natcher Conference Center (Building 45) on the NIH Campus, 45 Center Drive (off of Wisconsin Avenue/Rockville Pike), Bethesda, Maryland. 301-496-9966. There is a cafeteria at the Natcher Center which is open for breakfast and lunch. The Natcher Center is accessible for the physically challenged. Hotel Accommodations A block of rooms has been made available for conference attendees at the Hyatt Regency Hotel, 1 Bethesda Metro Center, Bethesda, MD, at the rates of $110.71 for single occupancy, $135.71 for double occupancy, plus 12% tax. Attendees are responsible for their own expenses while attending the conference and for making their own hotel and other reservations. If you wish to take advantage of the reduced rate at the Hyatt for conference attendees, call the hotel at 301-657-1234 or 800-233-1234 and tell the clerk you are attending the Scientific and Technical Data Exchange and Integration meeting. Hotel reservations must be made before November 13, 1997 in order to assure the special room rate. The Hyatt Regency is located next to the Bethesda Metro (subway) station, which can be used from National Airport and to the Natcher Center (see Metro directions below). The hotel is at the intersection of Wisconsin Avenue and Old Georgetown Road, 2.5 miles inside the Capital Beltway (I-95/I-495). Check-in time at the Hyatt is 3:00 pm, and luggage storage is available for guests arriving prior to check in. Check-out time is 12:00 noon. Local Transportation and Parking The Natcher Center and NIH have extremely limited parking, and parking spaces are not guaranteed. It is highly recommended for attendees to use the Metro or car pool. Cars parked in 3-hour spaces in front of the Natcher Center will be ticketed after 3 hours. Handicapped-tagged cars can be accommodated with day of event notice by request directly to the conference center business office. Driving Directions Interstate 495 Westbound: Take exit 33B (south, Connecticut Avenue). At 2nd traffic light, turn right onto Jones Bridge Road and proceed 2 more traffic lights to the intersection of Rockville Pike. Travel through the intersection onto Center Drive, make 3rd left and follow signs to parking lot 41B. Interstate 495 Eastbound: Take exit 34B (south, Bethesda/Wisconsin Avenue). Proceed 2 miles south on Rockville Pike. At 5th traffic light, turn right onto Center Drive, make 3rd left and follow signs to parking lot 41B. Wisconsin Avenue, from the District of Columbia: Proceed north from the District to 9000 Rockville Pike (Wisconsin Avenue). Turn left onto Center Drive (1st traffic light after Ramada Inn). Make 3rd left and follow signs to parking lot 41B. Metro >From the Hyatt Regency Hotel or downtown DC, take the Metrorail Red Line (in the direction of Shady Grove) to the Medical Center station, which is located on the NIH campus. The hotel is located only one metro stop from the conference site. Exit the Metro station via the escalator. At the top of the escalator (street level), turn left and follow the path (with signs) to the Natcher Center. You will see the building from the station. The path is ramped to accommodate the physically challenged. Organizing Committee Goetz Oertel (Chair), Association of Universities for Research in Astronomy Gerald Barton, National Oceanic and Atmospheric Administration Barbara Bauldock, Department of Energy James Beach, National Science Foundation W. Murray Black, George Mason University Rita Colwell, University of Maryland Biotechnology Institute Sara Graves, University of Alabama at Huntsville Stephen Koslow, National Institutes of Health Micah Krichevsky, Bionomics International David Lide, Jr., Consultant Kurt Molholm, Defense Technical Information Center Hedy Rossmeissl, United States Geological Survey John Rumble, National Institute of Standards and Technology James Thieman, National Aeronautics and Space Administration Paul Uhlir, Director, U.S. National Committee for CODATA, National Research Council Program Committee John Rumble (Chair), National Institute of Standards and Technology Martin Hardwick, STEP Tools, Inc. Julian Humphries, University of Kansas Paul Kanciruk, Oak Ridge National Laboratory David Mark, National Center for Geographic Information and Analysis Crystal Newton, Materials Sciences Corporation Robert Robbins, Fred Hutchinson Cancer Research Center Gio Wiederhold, Stanford University Conference Registration Space is limited and advance registration is required. To register, please complete the form (below), detach, and mail, enclosing a non-refundable registration fee. Registration fee: Before November 15, 1997 $150.00 After November 15, 1997 $200.00 Students $ 30.00 Please send only one registration per form. For other participants, reproduce the form prior to completing it. Only checks, money orders, or purchase orders can be accepted. We regret that we cannot take reservations by email and that we cannot accept credit cards. If you have special dietary or physical needs, please notify us in writing when registering. Need more information? Call: (202) 334-2421, or Email: CODATACO@NAS.EDU (cut here) Registration for Conference on Scientific and Technical Data Exchange and Integration (One form per registrant only--please print or type) Full Name: Title: Affiliation: Mailing Address Street: City: State: Zip code: Country: Telephone: Electronic mail address: Please indicate below if you have any dietary or physical restrictions, or have other comments: Please make check payable to: U.S. National Committee for CODATA. Mail to: U.S. National Committee for CODATA National Research Council, Room 315 2101 Constitution Avenue, NW Washington, DC 20418 U.S.A. From owner-structural-nmr@net.bio.net Fri Sep 26 23:00:00 1997 Path: biosci!biosci!not-for-mail From: JLEE@uga.cc.uga.edu Newsgroups: bionet.structural-nmr Subject: Please post this announcement Date: 27 Sep 1997 11:32:43 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 33 Sender: daemon@net.bio.net Approved: raman@hemebase.bio.uci.edu Distribution: world Message-ID: <970925.161435.EDT.JLEE@UGA.CC.UGA.EDU> NNTP-Posting-Host: net.bio.net Assistant Professor Structural Biology/NMR University of Georgia As part of a major structural biology initiative, the Departments of Chemistry and Biochemistry & Molecular Biology at the University of Georgia invite applications for a joint tenure-track faculty position at the ASSISTANT PROFESSOR level starting July 1, 1998. Preference will be given to candidates with experience and research interests in the area of solution structure and dynamics of biological macromolecules by NMR. Resources are available to attract candidates who are poised to contribute immediately and significantly to structural biology applications of NMR spectroscopy. These resources include acquisition of a 600 MHz NMR spectrometer as part of our structural biology initiative, and an 800 MHz spectrometer which is supported by the Georgia Research Alliance as a regional facility. Applicants should possess a Ph.D. in chemistry, biochemistry or closely related field, the potential to establish a vigorous independent research program, and the ability to contribute to teaching at both undergraduate and graduate levels. Postdoctoral experience is desirable. Applicants should send a curriculum vitae, a statement of teaching and research plans and arrange to have three letters of reference sent to: Professors Donald M. Kurtz, Jr. and James H. Prestegard, Co-chairs, NMR Search Committee, Department of Chemistry, University of Georgia, Athens, Georgia 30602-2556. Deadline for receipt of applications is November 1, 1997. The University of Georgia is an AA/EEO institution. We encourage applications from minorities and women. John Lee Department of Biochemistry University of Georgia Athens GA 30602-7229 USA Phone 706-542-1764 FAX 706-542-1738 From owner-structural-nmr@net.bio.net Fri Sep 26 23:00:00 1997 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.structural-nmr Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 27 Sep 1997 11:40:47 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 232 Sender: daemon@net.bio.net Approved: raman@hemebase.bio.uci.edu Distribution: world Message-ID: <199709220900.CAA28555@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-structural-nmr@net.bio.net Fri Sep 26 23:00:00 1997 Path: biosci!biosci!not-for-mail From: "Andre' Pampel" Newsgroups: bionet.structural-nmr Subject: Tryptophane-d12 available Date: 27 Sep 1997 11:37:07 -0700 Organization: University of Leipzig Lines: 14 Sender: daemon@net.bio.net Approved: raman@hemebase.bio.uci.edu Distribution: world Message-ID: <60jjoj$1mb@net.bio.net> NNTP-Posting-Host: net.bio.net Is anybody interested to get 2H totally labelled tryptophane? There are 9g left in our lab, which we would like to sell. Cheers Andre' ----------------------------------------------------------------- Andre' Pampel University of Leipzig Faculty of Physics and Geosciences Linnestrasse 5 04103 Leipzig Tel/Fax.: ++49-341-9732478/ ++49-341-9732479 email: anpa@rz.uni-leipzig.de http://www.uni-leipzig.de ----------------------------------------------------------------- From owner-structural-nmr@net.bio.net Fri Sep 26 23:00:00 1997 Path: biosci!biosci!not-for-mail From: "Zatonskiy George V." Newsgroups: bionet.structural-nmr Subject: nmrview Date: 27 Sep 1997 11:32:06 -0700 Organization: Institute of Organic Chemistry Russian Academy of Science Lines: 15 Sender: daemon@net.bio.net Approved: raman@hemebase.bio.uci.edu Distribution: world Message-ID: <342BA655.2287@nmr1.ioc.ac.ru> NNTP-Posting-Host: net.bio.net Dear netters, does anybody know about nmrview www site? The link to www.ges.com/nmrview/ is aged. Many thanks, George -- George V.Zatonskiy NMR laboratory N.D. Zelinsky Institute of Organic Chemistry, Leninsky pr., 47 Moscow 117913 Russia e-mail: zatonsk@nmr1.ioc.ac.ru tel.: (7-095) 135-9094 fax : (7-095) 135-5328 From owner-structural-nmr@net.bio.net Fri Sep 26 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Ouwen Zhang Newsgroups: bionet.structural-nmr Subject: expression system for peptides Date: 27 Sep 1997 11:31:25 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 22 Sender: daemon@net.bio.net Approved: raman@hemebase.bio.uci.edu Distribution: world Message-ID: <199709262159.OAA12843@oberon.scripps.edu> NNTP-Posting-Host: net.bio.net Hi netters, Novagen now supplies an expression system for recombinat peptides based on work from C.Walsh (JACS,116, 4599-4607, 1994). I also heard that there is another strategy in Peter Kim's lab to obtain recombinat peptides as well. I could not find the reference. Could anybody tell me about that ? Thanks Ouwen ***************************************** Ouwen Zhang Department of Molecular Biology, MB-2 The Scripps Research Institute 10550 N. Torrey Pines Road La Jolla, CA 92037 e-mail: ozhang@scripps.edu office phone (619) 784-9656 or 784-2800 NMR lab phone (619) 784-7455 fax (619) 784-9822 ***************************************** From owner-structural-nmr@net.bio.net Sat Sep 27 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Istvan Pelczer Newsgroups: bionet.structural-nmr Subject: Re: nmrview Date: 28 Sep 1997 13:14:18 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Approved: raman@hemebase.bio.uci.edu Distribution: world Message-ID: Reply-To: Istvan Pelczer NNTP-Posting-Host: net.bio.net Dear Netters, The view website is down for temporary time, but anyone can access the latest version at: ftp.ges.com in /pub/client/nmrview/3.0 via anonymous ftp. Should either of those interested experience a failure with the transfer, I shall be happy to send a copy over by email. Cheers, Istvan wwww,wwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwww Istvan Pelczer, Ph.D. Email: ipelczer@princeton.edu Senior NMR Spectroscopist Princeton University Department of Chemistry, Frick Lab., ph# (609) 258 2342 Washington Road fax# (609) 258 6746 Princeton, NJ 08544, USA On Fri, 26 Sep 1997, Zatonskiy George V. wrote: > Dear netters, > does anybody know about nmrview www site? > The link to www.ges.com/nmrview/ is aged. > Many thanks, > George > -- > George V.Zatonskiy > NMR laboratory > N.D. Zelinsky Institute of Organic Chemistry, > Leninsky pr., 47 > Moscow 117913 > Russia > e-mail: zatonsk@nmr1.ioc.ac.ru > tel.: (7-095) 135-9094 > fax : (7-095) 135-5328 > From owner-structural-nmr@net.bio.net Sun Sep 28 23:00:00 1997 Path: biosci!biosci!not-for-mail From: eshuang@biochem.wustl.edu (Enoch S. Huang) Newsgroups: bionet.structural-nmr Subject: NMR and X-ray homologues Date: 28 Sep 1997 20:02:26 -0700 Organization: Washington University School of Medicine Lines: 12 Sender: daemon@net.bio.net Approved: raman@hemebase.bio.uci.edu Distribution: world Message-ID: <60n5o2$f7g@net.bio.net> NNTP-Posting-Host: net.bio.net I am interested in finding pairs of structures in which one was solved by NMR and the other by X-ray crystallography -- but pairs of homologues (the more distant, the better) NOT the same protein. Can anyone suggest some pairs for me? Thanks! -- Enoch -- Enoch S. Huang, Ph.D. Washington University School of Medicine eshuang@biochem.wustl.edu From owner-structural-nmr@net.bio.net Sun Sep 28 23:00:00 1997 Path: biosci!biosci!not-for-mail From: David Cowburn Newsgroups: bionet.structural-nmr Subject: NMR SPECTROSCOPIST POSITION AT ROCKEFELLER UNIVERSITY Date: 29 Sep 1997 15:50:41 -0700 Organization: Rockefeller University Lines: 80 Sender: daemon@net.bio.net Approved: raman@hemebase.bio.uci.edu Distribution: world Message-ID: <34302F5F.531931A0@mr0.rockefeller.edu> NNTP-Posting-Host: net.bio.net --------------C6EDA59CE47CD31A54010792 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit The following ad will appear shortly in C&E News & similar. We would appreciate your bringing it to the attention of candidates. NMR Spectroscopist Skilled spectroscopist needed to support programs in Structural Biology, Chemistry, & Biochemistry at The Rockefeller University, using 600, 500, & 400 MHz spectrometers, and ancillary spectroscopy equipment. Candidates, preferably with a Ph.D. should be skilled in modern multidimensional NMR, familiar with maintenance and support of spectrometers, willing to involve themselves in a variety of stimulating and challenging technical research problems, and interested in the education of graduate and postdoctoral fellows in the use of NMR. There is a competitive salary, and the outstanding, stimulating environment of the research programs at Rockefeller (http://www.rockefeller.edu). Please apply to Dr. David Cowburn, fax 1 212 327 7566. Electronic mail applications will be welcome to dcadmin@mriris.rockefeller.edu. There is no discrimination against any employee applicant on the basis of race, color, religion, sex, national origin, or disability. --------------C6EDA59CE47CD31A54010792 Content-Type: text/html; charset=us-ascii Content-Transfer-Encoding: 7bit   
The following ad will appear shortly in C&E News & similar.  We would
appreciate your bringing it to the attention of candidates.
 
NMR Spectroscopist

Skilled spectroscopist needed to support programs in Structural Biology,
Chemistry, & Biochemistry at The Rockefeller University, using 600, 500, & 400
MHz spectrometers, and ancillary spectroscopy equipment.  Candidates, preferably with a Ph.D.
should be skilled in modern multidimensional NMR, familiar with maintenance and support
of spectrometers,  willing to involve themselves in a variety of stimulating
and challenging technical research problems, and interested in the education of graduate
and postdoctoral fellows in the use of NMR.  There is a competitive salary, and
the outstanding, stimulating environment of the research programs at
Rockefeller (http://www.rockefeller.edu).

Please apply to Dr. David Cowburn, fax 1 212 327 7566.  Electronic mail applications will be
welcome to dcadmin@mriris.rockefeller.edu.

There is no discrimination against any employee applicant on the basis of race,
color, religion, sex, national origin, or disability.
  --------------C6EDA59CE47CD31A54010792--