From owner-structural-nmr@net.bio.net Sat May 22 15:08:00 1999
Path: biosci!biosci!not-for-mail
From: "Qiang Tu" <suhoiy@hotmail.com>
Newsgroups: bionet.structural-nmr
Subject: NMR of GST fusion protein: advices needed
Date: 22 May 1999 09:08:35 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
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Dear all:

I am an novice of protein analysis and my research plan
is to express, purify and NMR a small protein. The protein
is not stable in E.coli since it's only 5 KD. Now I have
obtained the fusion protein with GST(26KD).

My question is:

Is it easy to purify, remove GST and get my target protein?
How can I determine the conformation of the target protein
	is native and stable since it is expressed in E.coli?
What else must I do before going on to NMR?

Would you please give me some advices and experience? I am
anxious of my experiments and looking foward to your reply.
Thank you very much!

Qiang Tu
tuqiangk@online.sh.cn