From owner-str-nmr@hgmp.mrc.ac.uk Mon Jan 10 15:01:41 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 0C0ED17A69; Mon, 10 Jan 2000 15:01:39 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 9741C17A62; Mon, 10 Jan 2000 15:01:38 +0000 (GMT) To: nobody@net.bio.net X-Really-To: str-nmr@net.bio.net Newsgroups: bionet.structural-nmr From: g.a.morris@man.ac.uk (Gareth Morris) Subject: Postdoc position available, Manchester UK Date: 10 Jan 2000 12:26:21 -0000 Organization: MRC Human Genome Mapping Project Resource Centre Message-ID: Sender: owner-str-nmr@hgmp.mrc.ac.uk Precedence: bulk If you know of any qualified candidates, I would be most grateful if you would draw this to their attention. Please excuse the cross-posting. Gareth Morris
Postdoctoral research associateship in NMR
A two year EPSRC postdoc position is available for work on high resolution diffusion-ordered spectroscopy (DOSY) with Prof GA Morris in the Department of Chemistry, University of Manchester. Further details may be found at http://www.ch.man.ac.uk/phys/NMR/PDRA677_99.htm. ________________________________________________________________________________ Prof. G.A. Morris g.a.morris@man.ac.uk Dept. of Chemistry, University of Manchester, Oxford Road, Manchester M13 9PL, UK (0) 161 275 4665 ________________________________________________________________________________ --- From owner-str-nmr@hgmp.mrc.ac.uk Thu Jan 20 01:43:38 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id BEEE417A6E; Thu, 20 Jan 2000 01:43:36 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id EBAD517A60; Thu, 20 Jan 2000 01:43:34 +0000 (GMT) To: nobody@net.bio.net X-Really-To: str-nmr@net.bio.net Newsgroups: bionet.structural-nmr From: rama@protein.chem.psu.edu (Ramakrishna V.) Subject: N15 Leu Specific labeling Date: 19 Jan 2000 23:59:18 -0000 Organization: MRC Human Genome Mapping Project Resource Centre Message-ID: <200001192359.SAA00499@protein.chem.psu.edu> Sender: owner-str-nmr@hgmp.mrc.ac.uk Precedence: bulk dear netters, i would like to know the effect of using BL21/DE3 E.coli strain for specifically labeling Leucine residues. I am interested in knowing to what other amino acids will the label be scambled. any information in this regard will be greatly appreciated. thanking you in advance ram PS: i have come across a paper in which N15 Leu labeling was performed using E.Coli JM107 strain. in this case the label was scrambled to VAL and ILE and to a lesser extent ALA. does the scambling of label depend on the strain used? From owner-str-nmr@hgmp.mrc.ac.uk Mon Jan 24 04:01:26 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 65F2C17B3B; Mon, 24 Jan 2000 04:01:25 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 3772C17A5A; Mon, 24 Jan 2000 04:01:24 +0000 (GMT) To: nobody@net.bio.net X-Really-To: str-nmr@net.bio.net Newsgroups: bionet.structural-nmr From: cns@cns.csb.yale.edu Subject: CNSsolve general release 1.0 Date: 23 Jan 2000 20:42:25 -0500 Organization: Yale University Message-ID: Sender: owner-str-nmr@hgmp.mrc.ac.uk Precedence: bulk ================================================================== Announcing version 1.0 (general release) of the software: Crystallography & NMR System (CNSsolve) (copyright 1997-2000, Yale University) ================================================================== Information about the software and instructions for downloading the most recent version are available at: ************************************************** ************ http://cns.csb.yale.edu ************* ************************************************** The software is available for download, free-of-charge, by all academic (non-profit) users. ------------------------------------------------------------------ Installation instructions and documentation can be found in: $CNS_SOLVE/doc/html/cns_solve.html once you have downloaded and installed the software. ------------------------------------------------------------------ Please cite the following reference for CNSsolve in publications: Brunger, A.T., Adams, P.D., Clore, G.M., Delano, W.L., Gros, P., Grosse-Kunstleve, R.W., Jiang, J.-S., Kuszewski, J., Nilges, M., Pannu, N.S., Read, R.J., Rice, L.M., Simonson, T., Warren, G.L. Crystallography & NMR system: A new software system for macromolecular structure determination, Acta Cryst. (1998). D54, 905-921 Please cite additional original papers when using specific methods. ------------------------------------------------------------------ Do not distribute CNSsolve to third parties without approval. By downloading the software you agree to the License in the FTP directory. ------------------------------------------------------------------ Attached release notes for CNSsolve version 1.0: ======================================================================= = = = Crystallography & NMR System = = = = A.T.Brunger, P.D.Adams, G.M.Clore, W.L.Delano, P.Gros, = = R.W.Grosse-Kunstleve, J.-S.Jiang, J.Kuszewski, M.Nilges, = = N.S.Pannu, R.J.Read, L.M.Rice, T.Simonson, G.L.Warren = = = = Copyright (c) 1997-2000 Yale University = = = ======================================================================= Program: CNSsolve Version: 1.0 Patch level: 0 Status: general release Changes for version 1.0 ----------------------- Program: - increased field length for reporting cell dimensions read from map and mask files - more informative message when program cannot obtain enough memory - removed use of C preprocessor - faster automatic build of source directory Makefile HTML Interface: - numeric entries can be set to blank to allow cut and paste - error checking for blank numeric entries HTML Documentation: - added entries to FAQ - minor changes to fix typos in tutorials Task files: - fixed major bugs in: xtal_twin/*.inp (incorrect number of reflections selected in some spacegroups) - changes to: general/neighbours.inp (option to write out only residues close to the molecule of interest) xtal_phase/mad_phase.inp (write out f'/f'' values for each scattering type if multiple atom types are used) xtal_refine/bindividual.inp (improved restraint weight estimation) optimize_rweight.inp ( " ) refine.inp ( " ) water_pick.inp (better handling of picking with strict NCS) xtal_twin/bindividual_twin.inp (improved restraint weight estimation) water_pick_twin.inp (better handling of picking with strict NCS) - new files: general/mtf_to_psf.inp (convert MTF file to PSF file) general/molecule_extent.inp (calculate extent of a molecule in orthogonal Angstroms) Libraries: - none Utilities: - fixed bug in mtz_to_cns (writing 2 headers when using CCP4 version 3.5) - fixed bug in to_cns which rejected scalepack reflections when the intensity was equal to -1 ============================================================================